JPWO2022192504A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2022192504A5 JPWO2022192504A5 JP2023554343A JP2023554343A JPWO2022192504A5 JP WO2022192504 A5 JPWO2022192504 A5 JP WO2022192504A5 JP 2023554343 A JP2023554343 A JP 2023554343A JP 2023554343 A JP2023554343 A JP 2023554343A JP WO2022192504 A5 JPWO2022192504 A5 JP WO2022192504A5
- Authority
- JP
- Japan
- Prior art keywords
- chromatography
- column
- pool
- polish
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004587 chromatography analysis Methods 0.000 claims 33
- 238000000034 method Methods 0.000 claims 22
- 238000010977 unit operation Methods 0.000 claims 18
- 238000001042 affinity chromatography Methods 0.000 claims 11
- 230000002779 inactivation Effects 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 8
- 230000003612 virological effect Effects 0.000 claims 8
- 238000006386 neutralization reaction Methods 0.000 claims 5
- 239000007853 buffer solution Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 3
- 230000003139 buffering effect Effects 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- 239000007983 Tris buffer Substances 0.000 claims 2
- 238000011067 equilibration Methods 0.000 claims 2
- 238000011068 loading method Methods 0.000 claims 2
- 230000003472 neutralizing effect Effects 0.000 claims 2
- 230000008929 regeneration Effects 0.000 claims 2
- 238000011069 regeneration method Methods 0.000 claims 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical group CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 1
- 101710120037 Toxin CcdB Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000006172 buffering agent Substances 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 238000005112 continuous flow technique Methods 0.000 claims 1
- 238000011118 depth filtration Methods 0.000 claims 1
- 238000011026 diafiltration Methods 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000012433 multimodal chromatography Methods 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 235000017281 sodium acetate Nutrition 0.000 claims 1
- 239000001632 sodium acetate Substances 0.000 claims 1
- 230000003068 static effect Effects 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
- 238000011100 viral filtration Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163159217P | 2021-03-10 | 2021-03-10 | |
| US63/159,217 | 2021-03-10 | ||
| PCT/US2022/019697 WO2022192504A1 (en) | 2021-03-10 | 2022-03-10 | Methods for purification of recombinant proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2024509877A JP2024509877A (ja) | 2024-03-05 |
| JP2024509877A5 JP2024509877A5 (https=) | 2025-03-10 |
| JPWO2022192504A5 true JPWO2022192504A5 (https=) | 2025-03-10 |
Family
ID=80952165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023554343A Pending JP2024509877A (ja) | 2021-03-10 | 2022-03-10 | 組換えタンパク質の精製方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20240051990A1 (https=) |
| EP (1) | EP4305048A1 (https=) |
| JP (1) | JP2024509877A (https=) |
| KR (1) | KR20230154908A (https=) |
| CN (1) | CN117043173A (https=) |
| AU (1) | AU2022234768A1 (https=) |
| CA (1) | CA3209216A1 (https=) |
| IL (1) | IL304571A (https=) |
| WO (1) | WO2022192504A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2024235093A1 (en) * | 2023-03-14 | 2025-09-04 | Amgen Inc. | Anion exchange chromatography processes using a primary amine ligand |
| US20240426793A1 (en) * | 2023-06-20 | 2024-12-26 | Amgen Inc. | Methods for chromatography and chromatography medium reuse |
| WO2025262319A1 (en) * | 2024-06-21 | 2025-12-26 | Boehringer Ingelheim International Gmbh | Modifying blue color formation in bioprocesses |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ302070B6 (cs) | 1998-04-21 | 2010-09-29 | Micromet Ag | Jednoretezcový multifunkcní polypeptid, polynukleotid, vektor obsahující tento polynukleotid, bunka transfekovaná tímto polynukleotidem, prostredek obsahující tento polypeptid, polynukleotid nebo vektor a jejich použití a zpusob identifikace aktiváto |
| DE602004030811D1 (de) | 2003-10-16 | 2011-02-10 | Micromet Ag | Multispezifische deimmunisierte cd3-bindende moleküle |
| RS53008B2 (sr) | 2007-04-03 | 2022-12-30 | Amgen Res Munich Gmbh | Interspecijski specifičan cd3-epsilon vezujući domen |
| EP2820047B1 (en) | 2012-03-01 | 2018-04-25 | Amgen Research (Munich) GmbH | Long life polypeptide binding molecules |
| EP2656892A1 (en) * | 2012-04-23 | 2013-10-30 | Merck Patent GmbH | Chromatography method |
| SG10201709131UA (en) * | 2013-03-08 | 2017-12-28 | Genzyme Corp | Continuous purification of therapeutic proteins |
| US20140302037A1 (en) | 2013-03-15 | 2014-10-09 | Amgen Inc. | BISPECIFIC-Fc MOLECULES |
| AU2014236769B2 (en) | 2013-03-15 | 2018-09-27 | Amgen Inc. | Heterodimeric bispecific antibodies |
| US20140308285A1 (en) | 2013-03-15 | 2014-10-16 | Amgen Inc. | Heterodimeric bispecific antibodies |
| WO2014140358A1 (en) | 2013-03-15 | 2014-09-18 | Amgen Research (Munich) Gmbh | Single chain binding molecules comprising n-terminal abp |
| EP3733710A1 (en) | 2013-09-25 | 2020-11-04 | Amgen, Inc | Hetrodimeric v-c-fc-v-c antibody |
| EA039859B1 (ru) | 2015-07-31 | 2022-03-21 | Эмджен Рисерч (Мюник) Гмбх | Биспецифические конструкты антител, связывающие egfrviii и cd3 |
-
2022
- 2022-03-10 JP JP2023554343A patent/JP2024509877A/ja active Pending
- 2022-03-10 CN CN202280019599.6A patent/CN117043173A/zh active Pending
- 2022-03-10 US US18/268,772 patent/US20240051990A1/en active Pending
- 2022-03-10 KR KR1020237033301A patent/KR20230154908A/ko active Pending
- 2022-03-10 AU AU2022234768A patent/AU2022234768A1/en active Pending
- 2022-03-10 CA CA3209216A patent/CA3209216A1/en active Pending
- 2022-03-10 EP EP22713202.4A patent/EP4305048A1/en active Pending
- 2022-03-10 WO PCT/US2022/019697 patent/WO2022192504A1/en not_active Ceased
-
2023
- 2023-07-19 IL IL304571A patent/IL304571A/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1571208B1 (en) | Novel factor IX purification methods | |
| KR101257330B1 (ko) | 펩타이드 정제 방법 | |
| AU2003232704A1 (en) | Method, use and kit for separating albumin from contaminants in a liquid | |
| RU2002117383A (ru) | Способ очистки фармакологически активных белков с помощью катионообменной хроматографии | |
| JPH0586097A (ja) | 上清ｉｖ、特にｉｖ−４またはコーンフラクシヨンｖまたはそれと類似の上清または画分からヒトアルブミンを単離する方法 | |
| US6001974A (en) | Method of separating albumin from serum by ion-exchange chromatography with membrane adsorbers | |
| Westra et al. | Immobilised metal-ion affinity chromatography purification of histidine-tagged recombinant proteins: a wash step with a low concentration of EDTA | |
| JP2002520338A5 (https=) | ||
| JPWO2022192504A5 (https=) | ||
| KR20190076046A (ko) | 단백질 용액의 탈염 공정 | |
| JP2573467B2 (ja) | 第ｉｘ因子タンパク質を含有する治療用途に適した薬剤的組成物 | |
| Fortis et al. | A new approach for the removal of protein impurities from purified biologicals using combinatorial solid‐phase ligand libraries | |
| CN1137292A (zh) | 借助于膜色谱进行的维生素k依赖性蛋白的纯化 | |
| CA2548165A1 (en) | Process for purifying interferon beta | |
| Mikulski et al. | Large scale purification of human fibroblast interferon | |
| JP7457023B2 (ja) | インスリンアナログの精製のためのクロマトグラフィープロセス | |
| EP4408561B1 (en) | Purification method and uses thereof | |
| Samaraweera et al. | Separation of insect hemolymph proteins by cascade‐mode multiaffinity chromatography | |
| SU551339A1 (ru) | Способ очистки ферментных препаратов | |
| JPH11335395A (ja) | 精製ヒトアクチビン及びその製造方法 | |
| JP2945181B2 (ja) | 免疫グロブリンｍの精製方法 | |
| KR20240135017A (ko) | 생물학적 제제를 정제하는 프로세스 중 오염물질의 선택적 제거를 위한 방법 | |
| Zhao et al. | Displacement chromatography with ion-exchangers | |
| RU2025474C1 (ru) | Способ получения s-сульфоната проинсулина | |
| JP2022538355A (ja) | Ｃ１－ｉｎｈを精製する方法 |