JPWO2022175655A5 - - Google Patents

Info

Publication number

JPWO2022175655A5

JPWO2022175655A5 JP2023549570A JP2023549570A JPWO2022175655A5 JP WO2022175655 A5 JPWO2022175655 A5 JP WO2022175655A5 JP 2023549570 A JP2023549570 A JP 2023549570A JP 2023549570 A JP2023549570 A JP 2023549570A JP WO2022175655 A5 JPWO2022175655 A5 JP WO2022175655A5

Authority

JP

Japan

Prior art keywords

target

oligonucleotide

nucleic acid

analyte

stranded

Prior art date

2025-02-07

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

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• 108091034117 Oligonucleotide Proteins 0.000 claims 24
• 239000000523 sample Substances 0.000 claims 21
• 239000012491 analyte Substances 0.000 claims 14
• 238000000034 method Methods 0.000 claims 14
• 108020004707 nucleic acids Proteins 0.000 claims 13
• 102000039446 nucleic acids Human genes 0.000 claims 13
• 150000007523 nucleic acids Chemical class 0.000 claims 13
• 239000011541 reaction mixture Substances 0.000 claims 10
• 230000000903 blocking effect Effects 0.000 claims 9
• 239000000047 product Substances 0.000 claims 9
• 102000004190 Enzymes Human genes 0.000 claims 7
• 108090000790 Enzymes Proteins 0.000 claims 7
• 230000003321 amplification Effects 0.000 claims 7
• 230000000295 complement effect Effects 0.000 claims 7
• 238000003199 nucleic acid amplification method Methods 0.000 claims 7
• 108091033319 polynucleotide Proteins 0.000 claims 6
• 102000040430 polynucleotide Human genes 0.000 claims 6
• 239000002157 polynucleotide Substances 0.000 claims 6
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 5
• 239000013067 intermediate product Substances 0.000 claims 5
• 102000003960 Ligases Human genes 0.000 claims 4
• 108090000364 Ligases Proteins 0.000 claims 4
• 238000001514 detection method Methods 0.000 claims 4
• 238000000137 annealing Methods 0.000 claims 3
• 239000012472 biological sample Substances 0.000 claims 3
• 230000029087 digestion Effects 0.000 claims 3
• 108060002716 Exonuclease Proteins 0.000 claims 2
• 229910019142 PO4 Inorganic materials 0.000 claims 2
• 102000013165 exonuclease Human genes 0.000 claims 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 2
• 239000010452 phosphate Substances 0.000 claims 2
• -1 pyrophosphate ions Chemical class 0.000 claims 2
• 235000011178 triphosphate Nutrition 0.000 claims 2
• 239000001226 triphosphate Substances 0.000 claims 2
• 102100034343 Integrase Human genes 0.000 claims 1
• 102000035195 Peptidases Human genes 0.000 claims 1
• 108091005804 Peptidases Proteins 0.000 claims 1
• 102000009609 Pyrophosphatases Human genes 0.000 claims 1
• 108010009413 Pyrophosphatases Proteins 0.000 claims 1
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
• 239000005547 deoxyribonucleotide Substances 0.000 claims 1
• 235000011180 diphosphates Nutrition 0.000 claims 1
• 239000000203 mixture Substances 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 239000003068 molecular probe Substances 0.000 claims 1
• 230000037452 priming Effects 0.000 claims 1
• 235000019833 protease Nutrition 0.000 claims 1
• 230000001841 pyrophosphorolytic effect Effects 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 1