JPWO2020081568A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2020081568A5 JPWO2020081568A5 JP2021545287A JP2021545287A JPWO2020081568A5 JP WO2020081568 A5 JPWO2020081568 A5 JP WO2020081568A5 JP 2021545287 A JP2021545287 A JP 2021545287A JP 2021545287 A JP2021545287 A JP 2021545287A JP WO2020081568 A5 JPWO2020081568 A5 JP WO2020081568A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- virus
- deaminase
- mutated
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 88
- 102000004169 proteins and genes Human genes 0.000 claims description 74
- 239000002773 nucleotide Substances 0.000 claims description 46
- 125000003729 nucleotide group Chemical group 0.000 claims description 46
- 241000700605 Viruses Species 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 29
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims description 16
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 16
- 230000035772 mutation Effects 0.000 claims description 16
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 claims description 8
- 102000055025 Adenosine deaminases Human genes 0.000 claims description 8
- 102100026846 Cytidine deaminase Human genes 0.000 claims description 8
- 108010031325 Cytidine deaminase Proteins 0.000 claims description 8
- 102000002488 Nucleoplasmin Human genes 0.000 claims description 8
- 239000003112 inhibitor Substances 0.000 claims description 8
- 108060005597 nucleoplasmin Proteins 0.000 claims description 8
- 229940035893 uracil Drugs 0.000 claims description 8
- 241000702421 Dependoparvovirus Species 0.000 claims description 7
- 241000972680 Adeno-associated virus - 6 Species 0.000 claims description 4
- 241001164825 Adeno-associated virus - 8 Species 0.000 claims description 4
- 102000003425 Tyrosinase Human genes 0.000 claims description 2
- 108060008724 Tyrosinase Proteins 0.000 claims description 2
- 201000011296 tyrosinemia Diseases 0.000 claims description 2
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 8
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 8
- 102200097286 rs199472825 Human genes 0.000 description 4
- 108091033409 CRISPR Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024107552A JP2024121000A (ja) | 2018-10-15 | 2024-07-03 | Ｎｍｅ２Ｃａｓ９－デアミナーゼ融合タンパク質によるブログラム可能なＤＮＡ塩基編集 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862745666P | 2018-10-15 | 2018-10-15 | |
| US62/745,666 | 2018-10-15 | ||
| PCT/US2019/056341 WO2020081568A1 (en) | 2018-10-15 | 2019-10-15 | Programmable dna base editing by nme2cas9-deaminase fusion proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024107552A Division JP2024121000A (ja) | 2018-10-15 | 2024-07-03 | Ｎｍｅ２Ｃａｓ９－デアミナーゼ融合タンパク質によるブログラム可能なＤＮＡ塩基編集 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2022508716A JP2022508716A (ja) | 2022-01-19 |
| JPWO2020081568A5 true JPWO2020081568A5 (https=) | 2022-09-20 |
| JP2022508716A5 JP2022508716A5 (https=) | 2022-09-20 |
| JP7588856B2 JP7588856B2 (ja) | 2024-11-25 |
Family
ID=68621349
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021545287A Active JP7588856B2 (ja) | 2018-10-15 | 2019-10-15 | Ｎｍｅ２Ｃａｓ９－デアミナーゼ融合タンパク質によるブログラム可能なＤＮＡ塩基編集 |
| JP2024107552A Pending JP2024121000A (ja) | 2018-10-15 | 2024-07-03 | Ｎｍｅ２Ｃａｓ９－デアミナーゼ融合タンパク質によるブログラム可能なＤＮＡ塩基編集 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024107552A Pending JP2024121000A (ja) | 2018-10-15 | 2024-07-03 | Ｎｍｅ２Ｃａｓ９－デアミナーゼ融合タンパク質によるブログラム可能なＤＮＡ塩基編集 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US12577546B2 (https=) |
| EP (1) | EP3867367A1 (https=) |
| JP (2) | JP7588856B2 (https=) |
| KR (1) | KR20210077732A (https=) |
| CN (1) | CN113166743B (https=) |
| BR (1) | BR112021007123A2 (https=) |
| CA (1) | CA3116555A1 (https=) |
| CO (1) | CO2021006336A2 (https=) |
| EA (1) | EA202191033A1 (https=) |
| IL (2) | IL282267B2 (https=) |
| MX (1) | MX2021004301A (https=) |
| MY (1) | MY209774A (https=) |
| PH (1) | PH12021550854A1 (https=) |
| SG (1) | SG11202103722TA (https=) |
| WO (1) | WO2020081568A1 (https=) |
| ZA (1) | ZA202103277B (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| JP7657726B2 (ja) | 2019-03-19 | 2025-04-07 | ザ ブロード インスティテュート，インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| GB202010348D0 (en) | 2020-07-06 | 2020-08-19 | Univ Wageningen | Base editing tools |
| CA3204997A1 (en) | 2020-12-11 | 2022-06-16 | Intellia Therapeutics, Inc. | Compositions and methods for reducing mhc class ii in a cell |
| CR20230305A (es) | 2020-12-11 | 2023-11-10 | Intellia Therapeutics Inc | Polinucleótidos, composiciones y métodos para la edición del genoma que implican desaminación |
| AU2021409732A1 (en) | 2020-12-23 | 2023-07-20 | Intellia Therapeutics, Inc. | Compositions and methods for reducing hla-a in a cell |
| TW202242101A (zh) | 2020-12-23 | 2022-11-01 | 美商英特利亞醫療公司 | 用於細胞中基因修飾ciita之組合物及方法 |
| EP4426821A1 (en) * | 2021-11-02 | 2024-09-11 | University of Massachusetts | Nme2cas9 inlaid domain fusion proteins |
| EP4426822A2 (en) | 2021-11-03 | 2024-09-11 | Intellia Therapeutics, Inc. | Polynucleotides, compositions, and methods for genome editing |
| AU2023248451A1 (en) * | 2022-04-04 | 2024-10-17 | President And Fellows Of Harvard College | Cas9 variants having non-canonical pam specificities and uses thereof |
| WO2023227669A2 (en) * | 2022-05-26 | 2023-11-30 | UCB Biopharma SRL | Novel nucleic acid-editing proteins |
| EP4540389A2 (en) | 2022-06-16 | 2025-04-23 | Intellia Therapeutics, Inc. | Compositions and methods for reducing mhc class i in a cell |
| CN119384498A (zh) | 2022-06-16 | 2025-01-28 | 因特利亚治疗公司 | 用于对细胞进行遗传修饰的方法和组合物 |
| EP4540388A2 (en) | 2022-06-16 | 2025-04-23 | Intellia Therapeutics, Inc. | Compositions and methods for genomic editing |
| CN115206456B (zh) * | 2022-07-13 | 2023-04-25 | 黑龙江大学 | 基于属性编辑流的分子生成方法 |
| CN115851775B (zh) * | 2022-10-18 | 2023-08-04 | 哈尔滨工业大学 | 一种Cas9蛋白抑制剂及其应用 |
| CN115716880A (zh) * | 2022-12-07 | 2023-02-28 | 云舟生物科技(广州)股份有限公司 | 一种核定位荧光蛋白及其应用 |
| WO2024138189A2 (en) | 2022-12-22 | 2024-06-27 | Intellia Therapeutics, Inc. | Methods for analyzing nucleic acid cargos of lipid nucleic acid assemblies |
| WO2024186890A1 (en) | 2023-03-06 | 2024-09-12 | Intellia Therapeutics, Inc. | Compositions and methods for hepatitis b virus (hbv) genome editing |
| US20240417707A1 (en) * | 2023-05-11 | 2024-12-19 | University Of Massachusetts | Compositions and methods for improved genome editing with nme2cas9 and nme2-smucas9 variants |
| AU2024324870A1 (en) | 2023-08-14 | 2026-02-12 | Intellia Therapeutics, Inc. | Compositions and methods for genetically modifying transforming growth factor beta receptor type 2 (tgfβr2) |
| TW202521564A (zh) | 2023-08-14 | 2025-06-01 | 美商英特利亞醫療公司 | 用於基於細胞之療法的ｃｄ７０ ｃａｒ－ｔ組合物及方法 |
| TW202515994A (zh) | 2023-08-14 | 2025-04-16 | 美商英特利亞醫療公司 | 用於對ｃｄ７０進行基因修飾之組合物及方法 |
| WO2025137301A1 (en) | 2023-12-20 | 2025-06-26 | Intellia Therapeutics, Inc. | Methods for rapid engineering of cells |
| WO2025255308A1 (en) | 2024-06-07 | 2025-12-11 | Intellia Therapeutics, Inc. | Cd8 co-receptor chimeric polypeptides in tcr cell therapy |
| WO2026014914A1 (ko) * | 2024-07-10 | 2026-01-15 | 서울대학교산학협력단 | Adar 효소 또는 이의 변이체를 이용한 단일 염기 교정기 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| MX374090B (es) * | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| JP7109784B2 (ja) * | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したＣａｓ９蛋白質 |
| KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| WO2018152197A1 (en) * | 2017-02-15 | 2018-08-23 | Massachusetts Institute Of Technology | Dna writers, molecular recorders and uses thereof |
| KR102772726B1 (ko) | 2017-02-15 | 2025-02-25 | 키진 엔.브이. | 식물 세포에서 표적 유전적 변형 방법 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
-
2019
- 2019-10-15 BR BR112021007123-7A patent/BR112021007123A2/pt unknown
- 2019-10-15 IL IL282267A patent/IL282267B2/en unknown
- 2019-10-15 CN CN201980077403.7A patent/CN113166743B/zh active Active
- 2019-10-15 KR KR1020217014669A patent/KR20210077732A/ko active Pending
- 2019-10-15 MY MYPI2021002031A patent/MY209774A/en unknown
- 2019-10-15 US US17/285,440 patent/US12577546B2/en active Active
- 2019-10-15 WO PCT/US2019/056341 patent/WO2020081568A1/en not_active Ceased
- 2019-10-15 PH PH1/2021/550854A patent/PH12021550854A1/en unknown
- 2019-10-15 CA CA3116555A patent/CA3116555A1/en active Pending
- 2019-10-15 SG SG11202103722TA patent/SG11202103722TA/en unknown
- 2019-10-15 JP JP2021545287A patent/JP7588856B2/ja active Active
- 2019-10-15 EP EP19806344.8A patent/EP3867367A1/en active Pending
- 2019-10-15 MX MX2021004301A patent/MX2021004301A/es unknown
- 2019-10-15 EA EA202191033A patent/EA202191033A1/ru unknown
-
2021
- 2021-05-14 ZA ZA2021/03277A patent/ZA202103277B/en unknown
- 2021-05-14 CO CONC2021/0006336A patent/CO2021006336A2/es unknown
-
2024
- 2024-07-03 JP JP2024107552A patent/JP2024121000A/ja active Pending
-
2025
- 2025-09-26 IL IL323589A patent/IL323589A/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPWO2020081568A5 (https=) | ||
| JP2022508716A5 (https=) | ||
| Ogi et al. | Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB | |
| Andres et al. | cDNA cloning of component A of Rab geranylgeranyl transferase and demonstration of its role as a Rab escort protein | |
| Poison et al. | RNA editing of hepatitis delta virus antigenome by dsRNA-adenosine deaminase | |
| Mathews et al. | Adenovirus virus-associated RNA and translation control | |
| Lamm et al. | Non-snRNP protein splicing factors | |
| CA3011484A1 (en) | Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process | |
| Tyndall et al. | The Escherichia coli prr region encodes a functional type IC DNA restriction system closely integrated with an anticodon nutlease gene | |
| WO2016004010A1 (en) | Regulated gene expression from viral vectors | |
| Vestergaard et al. | A novel rudivirus, ARV1, of the hyperthermophilic archaeal genus Acidianus | |
| JP2019500043A5 (https=) | ||
| JP2010514418A (ja) | 腫瘍退縮アデノウイルスの作出およびその使用 | |
| Vestal | The guanylate-binding proteins (GBPs): proinflammatory cytokine-induced members of the dynamin superfamily with unique GTPase activity | |
| US20220162648A1 (en) | Compositions and methods for improved gene editing | |
| Shors et al. | Complementation of Deletion of the Vaccinia Virus E3L Gene by theEscherichia coliRNase III Gene | |
| Liu et al. | Mutational switching of a yeast tRNA synthetase into a mammalian-like synthetase cytokine | |
| JP7672006B2 (ja) | 制御性ｔ細胞を改変するための組成物および方法 | |
| AU685179B2 (en) | (Plasmodium falciparum) ribonucleotide reductase, encoding DNA, and inhibitors | |
| AU2776792A (en) | Methods and compositions for identifying inhibitors of papilloma virus replication | |
| Li et al. | Improvement of C‐to‐U RNA editing using an artificial MS2‐APOBEC system | |
| WO2024245139A1 (en) | Gene editing systems and uses thereof | |
| Jayachandran et al. | The mosquito ribonucleotide reductase R2 gene: ultraviolet light induces expression of a novel R2 variant with an internal amino acid deletion | |
| EP1147218B1 (en) | Selection procedure using prodrug/enzyme system | |
| AU2003229543A1 (en) | Plant thymidine kinases and their use |