JPWO2020002598A5 - - Google Patents
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- JPWO2020002598A5 JPWO2020002598A5 JP2020567587A JP2020567587A JPWO2020002598A5 JP WO2020002598 A5 JPWO2020002598 A5 JP WO2020002598A5 JP 2020567587 A JP2020567587 A JP 2020567587A JP 2020567587 A JP2020567587 A JP 2020567587A JP WO2020002598 A5 JPWO2020002598 A5 JP WO2020002598A5
- Authority
- JP
- Japan
- Prior art keywords
- reaction vessel
- bioreactor
- dna
- magnetic particles
- appendix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006243 chemical reaction Methods 0.000 claims description 86
- 239000006249 magnetic particle Substances 0.000 claims description 60
- 230000005291 magnetic effect Effects 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 239000000872 buffer Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 238000000338 in vitro Methods 0.000 claims description 7
- 238000010584 magnetic trap Methods 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 230000033001 locomotion Effects 0.000 claims description 5
- 238000013518 transcription Methods 0.000 claims description 5
- 230000035897 transcription Effects 0.000 claims description 5
- 235000011178 triphosphate Nutrition 0.000 claims description 5
- 239000001226 triphosphate Substances 0.000 claims description 5
- 230000001419 dependent effect Effects 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 3
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000002342 ribonucleoside Substances 0.000 claims description 3
- 230000006820 DNA synthesis Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000002907 paramagnetic material Substances 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 description 48
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 26
- 239000011324 bead Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 101150077194 CAP1 gene Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 101100438378 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) fac-1 gene Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102000007327 Protamines Human genes 0.000 description 1
- 108010007568 Protamines Proteins 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- -1 modified ribonucleoside triphosphate Chemical class 0.000 description 1
- 239000002362 mulch Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940048914 protamine Drugs 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EPPCT/EP2018/067504 | 2018-06-28 | ||
| EP2018067504 | 2018-06-28 | ||
| PCT/EP2019/067323 WO2020002598A1 (en) | 2018-06-28 | 2019-06-28 | Bioreactor for rna in vitro transcription |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2021528051A JP2021528051A (ja) | 2021-10-21 |
| JP2021528051A5 JP2021528051A5 (https=) | 2022-01-21 |
| JPWO2020002598A5 true JPWO2020002598A5 (https=) | 2022-01-21 |
| JP7352581B2 JP7352581B2 (ja) | 2023-09-28 |
Family
ID=62837899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020567587A Active JP7352581B2 (ja) | 2018-06-28 | 2019-06-28 | Ｒｎａインビトロ転写用バイオリアクター |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US12371646B2 (https=) |
| EP (2) | EP4273266A3 (https=) |
| JP (1) | JP7352581B2 (https=) |
| KR (1) | KR20210027368A (https=) |
| CN (2) | CN112334579B (https=) |
| AU (1) | AU2019293714C1 (https=) |
| BR (1) | BR112020025601A8 (https=) |
| CA (1) | CA3102135A1 (https=) |
| IL (1) | IL279759B2 (https=) |
| MX (1) | MX2020013148A (https=) |
| RU (1) | RU2765877C1 (https=) |
| SG (1) | SG11202011097YA (https=) |
| WO (1) | WO2020002598A1 (https=) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012019630A1 (en) | 2010-08-13 | 2012-02-16 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein |
| WO2013120499A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen |
| RU2658490C2 (ru) | 2012-03-27 | 2018-06-21 | Кьюрвак Аг | Искусственные молекулы нуклеиновых кислот для улучшенной экспрессии белков или пептидов |
| AU2014310935B2 (en) | 2013-08-21 | 2019-11-21 | CureVac SE | Combination vaccine |
| BR112016001192A2 (pt) | 2013-08-21 | 2017-08-29 | Curevac Ag | Vacina contra a raiva |
| BR112016003361A2 (pt) | 2013-08-21 | 2017-11-21 | Curevac Ag | vacina do vírus sincicial respiratório (rsv) |
| ES2806575T3 (es) | 2013-11-01 | 2021-02-18 | Curevac Ag | ARN modificado con propiedades inmunoestimuladoras disminuidas |
| WO2015135558A1 (en) | 2014-03-12 | 2015-09-17 | Curevac Gmbh | Combination of vaccination and ox40 agonists |
| DE202015010000U1 (de) | 2014-12-12 | 2023-07-03 | CureVac SE | Artifizielle Nukleinsäuremoleküle für eine verbesserte Proteinexpression |
| DK3294885T3 (da) | 2015-05-08 | 2020-08-10 | Curevac Real Estate Gmbh | Fremgangsmåde til at fremstille rna |
| PL4108769T3 (pl) | 2015-05-29 | 2024-02-05 | CureVac Manufacturing GmbH | Sposób wytwarzania i oczyszczania rna obejmujący co najmniej jeden etap filtracji o przepływie stycznym |
| US11413346B2 (en) | 2015-11-09 | 2022-08-16 | Curevac Ag | Rotavirus vaccines |
| EP3701963A1 (en) | 2015-12-22 | 2020-09-02 | CureVac AG | Method for producing rna molecule compositions |
| US11723967B2 (en) | 2016-02-17 | 2023-08-15 | CureVac SE | Zika virus vaccine |
| EP3423595A1 (en) | 2016-03-03 | 2019-01-09 | CureVac AG | Rna analysis by total hydrolysis |
| EP3452101A2 (en) | 2016-05-04 | 2019-03-13 | CureVac AG | Rna encoding a therapeutic protein |
| EP4631970A3 (en) | 2016-05-04 | 2026-01-07 | CureVac SE | Nucleic acid molecules and uses thereof |
| WO2018115525A1 (en) | 2016-12-23 | 2018-06-28 | Curevac Ag | Lassa virus vaccine |
| EP3558356A2 (en) | 2016-12-23 | 2019-10-30 | CureVac AG | Mers coronavirus vaccine |
| AU2018240515B2 (en) | 2017-03-24 | 2024-07-25 | CureVac SE | Nucleic acids encoding CRISPR-associated proteins and uses thereof |
| WO2018211038A1 (en) | 2017-05-17 | 2018-11-22 | Curevac Ag | Method for determining at least one quality parameter of an rna sample |
| US11602557B2 (en) | 2017-08-22 | 2023-03-14 | Cure Vac SE | Bunyavirales vaccine |
| EP3707271A1 (en) | 2017-11-08 | 2020-09-16 | CureVac AG | Rna sequence adaptation |
| EP3723796A1 (en) | 2017-12-13 | 2020-10-21 | CureVac AG | Flavivirus vaccine |
| EP3728634A1 (en) | 2017-12-21 | 2020-10-28 | CureVac AG | Linear double stranded dna coupled to a single support or a tag and methods for producing said linear double stranded dna |
| CA3091558A1 (en) | 2018-04-17 | 2019-10-24 | Curevac Ag | Novel rsv rna molecules and compositions for vaccination |
| BR112020025601A8 (pt) | 2018-06-28 | 2022-07-05 | Tesla Automation GmbH | Biorreator para a transcrição in vitro de rna |
| US11421226B2 (en) * | 2018-10-17 | 2022-08-23 | Ramot At Tel-Aviv University Ltd. | Programmable artificial cell |
| TW202039534A (zh) | 2018-12-14 | 2020-11-01 | 美商美國禮來大藥廠 | KRAS變體mRNA分子 |
| US12492425B2 (en) | 2018-12-21 | 2025-12-09 | CureVac SE | Methods for RNA analysis |
| KR20220121246A (ko) | 2019-12-20 | 2022-08-31 | 큐어백 아게 | 핵산 전달용 신규한 지질 나노입자 |
| MX2022009460A (es) | 2020-02-04 | 2022-12-16 | Curevac Ag | Vacuna contra el coronavirus. |
| US12194089B2 (en) | 2020-02-04 | 2025-01-14 | CureVac SE | Coronavirus vaccine |
| US11241493B2 (en) | 2020-02-04 | 2022-02-08 | Curevac Ag | Coronavirus vaccine |
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| JP7789698B2 (ja) * | 2020-05-12 | 2025-12-22 | ハラランボス・マカソリス | Ｒｎａを用いた治療薬の合成方法及び合成モジュール式装置 |
| GB2597436B (en) * | 2020-05-12 | 2025-03-26 | Makatsoris Charalampos | A method and apparatus for RNA synthesis |
| MX2022015132A (es) | 2020-05-29 | 2023-03-08 | CureVac SE | Vacunas combinadas a base de acidos nucleicos. |
| CA3170743A1 (en) | 2020-08-31 | 2022-03-03 | Susanne RAUCH | Multivalent nucleic acid based coronavirus vaccines |
| US20240246056A1 (en) | 2020-09-01 | 2024-07-25 | CureVac RNA Printer GmbH | Manufacturing device for a pharmaceutical product |
| JP2023546175A (ja) | 2020-10-14 | 2023-11-01 | ジョージ・メイソン・リサーチ・ファウンデーション・インコーポレイテッド | 脂質ナノ粒子製造の方法及びそれに由来する組成物 |
| EP4232569B1 (en) * | 2020-10-23 | 2026-03-11 | LineaRX, Inc. | Compositions and methods for rna synthesis |
| JP7641487B2 (ja) | 2020-11-27 | 2025-03-07 | キュアバック マニュファクチャリング ゲーエムベーハー | キャピラリーポリメラーゼ連鎖反応によってｄｎａ産物を調製するためのデバイス |
| WO2022137133A1 (en) | 2020-12-22 | 2022-06-30 | Curevac Ag | Rna vaccine against sars-cov-2 variants |
| US20240156946A1 (en) | 2020-12-22 | 2024-05-16 | CureVac SE | Rna vaccine against sars-cov-2 variants |
| WO2022178357A1 (en) * | 2021-02-22 | 2022-08-25 | The Board Of Regents Of The University Of Texas System | Magnetic description magnetic shear bioreactor apparatus and methods |
| US20240181037A1 (en) | 2021-03-26 | 2024-06-06 | Glaxosmithkline Biologicals Sa | Immunogenic compositions |
| WO2022261050A1 (en) * | 2021-06-07 | 2022-12-15 | The University Of Massachusetts | Apparatus and method for continuous production of rna |
| EP4508440A1 (en) | 2022-04-15 | 2025-02-19 | Quantoom Biosciences S.A. | System and method for the production of rna |
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| CN115106356B (zh) * | 2022-07-06 | 2023-06-06 | 眉山市城投中恒能环保科技有限公司 | 一种用于餐厨垃圾回收的处理方法及处理系统 |
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-
2019
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- 2019-06-28 EP EP23175456.5A patent/EP4273266A3/en not_active Withdrawn
- 2019-06-28 RU RU2020141236A patent/RU2765877C1/ru active
- 2019-06-28 CA CA3102135A patent/CA3102135A1/en active Pending
- 2019-06-28 JP JP2020567587A patent/JP7352581B2/ja active Active
- 2019-06-28 EP EP19733798.3A patent/EP3814495B1/en active Active
- 2019-06-28 MX MX2020013148A patent/MX2020013148A/es unknown
- 2019-06-28 WO PCT/EP2019/067323 patent/WO2020002598A1/en not_active Ceased
- 2019-06-28 IL IL279759A patent/IL279759B2/en unknown
- 2019-06-28 SG SG11202011097YA patent/SG11202011097YA/en unknown
- 2019-06-28 CN CN201980043390.1A patent/CN112334579B/zh active Active
- 2019-06-28 KR KR1020217001173A patent/KR20210027368A/ko not_active Abandoned
- 2019-06-28 AU AU2019293714A patent/AU2019293714C1/en active Active
- 2019-06-28 US US17/254,853 patent/US12371646B2/en active Active
- 2019-06-28 CN CN202511910012.2A patent/CN121628760A/zh active Pending
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2025
- 2025-06-20 US US19/244,186 patent/US20250313788A1/en active Pending
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