JPWO2019133677A5 - - Google Patents
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- Publication number
- JPWO2019133677A5 JPWO2019133677A5 JP2020536656A JP2020536656A JPWO2019133677A5 JP WO2019133677 A5 JPWO2019133677 A5 JP WO2019133677A5 JP 2020536656 A JP2020536656 A JP 2020536656A JP 2020536656 A JP2020536656 A JP 2020536656A JP WO2019133677 A5 JPWO2019133677 A5 JP WO2019133677A5
- Authority
- JP
- Japan
- Prior art keywords
- buffer
- affinity resin
- trishcl
- elution
- hcl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000872 buffer Substances 0.000 claims description 122
- 238000000034 method Methods 0.000 claims description 120
- 239000011347 resin Substances 0.000 claims description 99
- 229920005989 resin Polymers 0.000 claims description 99
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 96
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 80
- 238000005406 washing Methods 0.000 claims description 59
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 44
- 239000011780 sodium chloride Substances 0.000 claims description 40
- 239000012149 elution buffer Substances 0.000 claims description 29
- 150000003839 salts Chemical class 0.000 claims description 29
- 239000007853 buffer solution Substances 0.000 claims description 28
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 24
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 24
- 238000010828 elution Methods 0.000 claims description 24
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 20
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 20
- 229920000053 polysorbate 80 Polymers 0.000 claims description 20
- 229940068968 polysorbate 80 Drugs 0.000 claims description 20
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 19
- 229960003589 arginine hydrochloride Drugs 0.000 claims description 19
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 18
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 17
- 239000001632 sodium acetate Substances 0.000 claims description 17
- 235000017281 sodium acetate Nutrition 0.000 claims description 17
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 230000002378 acidificating effect Effects 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 239000004094 surface-active agent Substances 0.000 claims description 9
- 229960003080 taurine Drugs 0.000 claims description 9
- 241001164825 Adeno-associated virus - 8 Species 0.000 claims description 8
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 claims description 8
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims description 8
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 6
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 6
- QZNNVYOVQUKYSC-JEDNCBNOSA-N (2s)-2-amino-3-(1h-imidazol-5-yl)propanoic acid;hydron;chloride Chemical compound Cl.OC(=O)[C@@H](N)CC1=CN=CN1 QZNNVYOVQUKYSC-JEDNCBNOSA-N 0.000 claims description 5
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 5
- 150000001450 anions Chemical class 0.000 claims description 5
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 5
- 229960003646 lysine Drugs 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Inorganic materials [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 4
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 4
- 229930195725 Mannitol Natural products 0.000 claims description 4
- 229920004890 Triton X-100 Polymers 0.000 claims description 4
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 claims description 4
- 239000012504 chromatography matrix Substances 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
- 235000010355 mannitol Nutrition 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- -1 polysolvate 80 Chemical compound 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000000600 sorbitol Substances 0.000 claims description 4
- 235000010356 sorbitol Nutrition 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 3
- IRHWMYKYLWNHTL-UHFFFAOYSA-M sodium 2-(N-morpholino)ethanesulfonate Chemical compound [Na+].[O-]S(=O)(=O)CCN1CCOCC1 IRHWMYKYLWNHTL-UHFFFAOYSA-M 0.000 claims description 3
- STCOOQWBFONSKY-UHFFFAOYSA-N tributyl phosphate Chemical compound CCCCOP(=O)(OCCCC)OCCCC STCOOQWBFONSKY-UHFFFAOYSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 claims description 2
- IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 241000702421 Dependoparvovirus Species 0.000 claims description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- DZTHIGRZJZPRDV-UHFFFAOYSA-N N-acetyltryptophan Chemical compound C1=CC=C2C(CC(NC(=O)C)C(O)=O)=CNC2=C1 DZTHIGRZJZPRDV-UHFFFAOYSA-N 0.000 claims description 2
- 239000008118 PEG 6000 Substances 0.000 claims description 2
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
- 229940093476 ethylene glycol Drugs 0.000 claims description 2
- 229960001855 mannitol Drugs 0.000 claims description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 239000008213 purified water Substances 0.000 claims description 2
- 159000000000 sodium salts Chemical class 0.000 claims description 2
- 229960002920 sorbitol Drugs 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000000811 xylitol Substances 0.000 claims description 2
- 235000010447 xylitol Nutrition 0.000 claims description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 2
- 229960002675 xylitol Drugs 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 2
- RYVMUASDIZQXAA-UHFFFAOYSA-N pyranoside Natural products O1C2(OCC(C)C(OC3C(C(O)C(O)C(CO)O3)O)C2)C(C)C(C2(CCC3C4(C)CC5O)C)C1CC2C3CC=C4CC5OC(C(C1O)O)OC(CO)C1OC(C1OC2C(C(OC3C(C(O)C(O)C(CO)O3)O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OCC(O)C(O)C1O RYVMUASDIZQXAA-UHFFFAOYSA-N 0.000 claims 1
- 238000002965 ELISA Methods 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 239000001166 ammonium sulphate Substances 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 229960002449 glycine Drugs 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 238000003118 sandwich ELISA Methods 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023072157A JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762611709P | 2017-12-29 | 2017-12-29 | |
| US62/611,709 | 2017-12-29 | ||
| PCT/US2018/067627 WO2019133677A1 (en) | 2017-12-29 | 2018-12-27 | Adeno-associated virus purification methods |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023072157A Division JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2021508484A JP2021508484A (ja) | 2021-03-11 |
| JPWO2019133677A5 true JPWO2019133677A5 (https=) | 2022-02-08 |
| JP2021508484A5 JP2021508484A5 (https=) | 2022-02-08 |
| JP7299896B2 JP7299896B2 (ja) | 2023-06-28 |
Family
ID=67064154
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020536656A Active JP7299896B2 (ja) | 2017-12-29 | 2018-12-27 | アデノ随伴ウイルス精製方法 |
| JP2023072157A Active JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023072157A Active JP7531654B2 (ja) | 2017-12-29 | 2023-04-26 | アデノ随伴ウイルス精製方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US11981934B2 (https=) |
| EP (1) | EP3731863A4 (https=) |
| JP (2) | JP7299896B2 (https=) |
| KR (1) | KR20200106045A (https=) |
| CN (1) | CN111655285B (https=) |
| AU (1) | AU2018395254B2 (https=) |
| BR (1) | BR112020013197A8 (https=) |
| CA (1) | CA3084827A1 (https=) |
| IL (1) | IL275533B2 (https=) |
| MX (1) | MX2020006884A (https=) |
| SG (1) | SG11202006232SA (https=) |
| WO (1) | WO2019133677A1 (https=) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019299964A1 (en) * | 2018-07-11 | 2021-01-21 | Takeda Pharmaceutical Company Limited | AAV compositions |
| EP3990031A4 (en) * | 2019-06-28 | 2023-08-09 | Takeda Pharmaceutical Company Limited | Adeno-associated virus purification methods |
| EP3868886A1 (en) * | 2020-02-21 | 2021-08-25 | Bia Separations D.O.O. | A method for separation or depletion of empty aav capsids from full aav capsids |
| US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
| JP2023539163A (ja) * | 2020-08-18 | 2023-09-13 | ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． | 宿主細胞ｄｎａの金属アフィニティー抽出 |
| JP2023548816A (ja) * | 2020-11-03 | 2023-11-21 | ファイザー・インク | 陰イオン交換クロマトグラフィーによるａａｖベクターの精製方法 |
| US20230416693A1 (en) * | 2020-11-24 | 2023-12-28 | Sartorius Bia Separations D.O.O. | Improved washing methods for affinity chromatography |
| JP2024503234A (ja) * | 2020-12-23 | 2024-01-25 | ファイザー・インク | 親和性クロマトグラフィーによるａａｖベクターの精製方法 |
| EP4281552A4 (en) * | 2021-01-21 | 2025-03-12 | AskBio Inc. | Method for purifying recombinant viral particles |
| WO2022222869A1 (zh) * | 2021-04-19 | 2022-10-27 | 信念医药（香港）有限公司 | 重组腺相关病毒及其应用 |
| CN114591921B (zh) * | 2022-03-31 | 2024-08-02 | 上海勉亦生物科技有限公司 | 腺相关病毒的纯化方法和洗脱液 |
| GB202110014D0 (en) * | 2021-07-12 | 2021-08-25 | Cytiva Bioprocess R & D Ab | A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof |
| CN113552349B (zh) * | 2021-07-30 | 2022-07-19 | 上海勉亦生物科技有限公司 | Aav蛋白外壳的检测方法 |
| US20230183659A1 (en) * | 2021-10-29 | 2023-06-15 | Homology Medicines, Inc. | Methods and compositions for the purification of adeno-associated virus |
| CN114350622A (zh) * | 2022-01-17 | 2022-04-15 | 苏州博腾生物制药有限公司 | 一种病毒纯化高效保护试剂及其应用 |
| EP4490179A1 (en) | 2022-03-07 | 2025-01-15 | Takeda Pharmaceutical Company Limited | Affinity chromatographic production of clinical human igg products |
| GB202206123D0 (en) * | 2022-04-27 | 2022-06-08 | Cytiva Bioprocess R & D Ab | A pre-screening method and a method for separating adeno-associated virus capsids |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
| WO2025027058A1 (en) * | 2023-08-01 | 2025-02-06 | Merck Patent Gmbh | Polishing for aav particles using anion exchange chromatography with improved elution system |
| WO2025104341A1 (en) * | 2023-11-16 | 2025-05-22 | Sartorius Bia Separations D.O.O. | Enhanced chromatographic separation of components in a mixture by employing decreasing gradient of complexing agent |
| WO2025207456A1 (en) * | 2024-03-25 | 2025-10-02 | Frontera Therapeutics, Inc. | Methods for purification of adeno associated virus particles by anion exchange chromatography |
| WO2026077794A1 (en) * | 2024-10-07 | 2026-04-16 | Cytiva Us Llc | Plasmid purification methods |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4543363A (en) | 1983-06-15 | 1985-09-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Ion exchanger having hydroxyl groups bonded directly to backbone skeleton |
| SE9902133D0 (sv) | 1999-06-06 | 1999-06-06 | Amersham Pharm Biotech Ab | A method for surface modification, a novel support matrix and the use of the matrix |
| GB9925432D0 (en) * | 1999-10-27 | 1999-12-29 | Univ London | Virus preparation |
| US6593123B1 (en) | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| HUE028341T2 (en) | 2009-06-16 | 2016-12-28 | Genzyme Corp | Improved methods for purification of recombinant aav vectors |
| US20150275195A1 (en) * | 2012-10-24 | 2015-10-01 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| CN104232687A (zh) * | 2014-09-17 | 2014-12-24 | 许瑞安 | 一种重组腺相关病毒rAAV载体的分离纯化方法 |
| EP3054007A1 (en) * | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step |
| WO2017100676A1 (en) | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav8 |
| EP3387138B1 (en) | 2015-12-11 | 2022-01-26 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav9 |
| KR20220100017A (ko) | 2019-11-21 | 2022-07-14 | 이엠디 밀리포어 코포레이션 | 친수성 막 |
-
2018
- 2018-12-27 SG SG11202006232SA patent/SG11202006232SA/en unknown
- 2018-12-27 EP EP18895008.3A patent/EP3731863A4/en active Pending
- 2018-12-27 IL IL275533A patent/IL275533B2/en unknown
- 2018-12-27 WO PCT/US2018/067627 patent/WO2019133677A1/en not_active Ceased
- 2018-12-27 CN CN201880087932.0A patent/CN111655285B/zh active Active
- 2018-12-27 US US16/958,974 patent/US11981934B2/en active Active
- 2018-12-27 MX MX2020006884A patent/MX2020006884A/es unknown
- 2018-12-27 CA CA3084827A patent/CA3084827A1/en active Pending
- 2018-12-27 BR BR112020013197A patent/BR112020013197A8/pt unknown
- 2018-12-27 JP JP2020536656A patent/JP7299896B2/ja active Active
- 2018-12-27 KR KR1020207021627A patent/KR20200106045A/ko not_active Ceased
- 2018-12-27 AU AU2018395254A patent/AU2018395254B2/en active Active
-
2023
- 2023-04-26 JP JP2023072157A patent/JP7531654B2/ja active Active
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