JPWO2007097433A1 - Deodorant composition - Google Patents

Abstract

An object of the present invention is to provide a deodorant composition having a significantly enhanced deodorizing effect when applied to chewing gum or under acidic conditions. According to the present invention, there is provided a deodorant composition comprising an extraction residue after extraction of a mint fragrance component and an oxidoreductase.

Description

  The present invention relates to a deodorant composition comprising an extraction residue after extraction of a mint flavor component and an oxidoreductase, and a chewing gum comprising the deodorant composition.

  Conventionally, various deodorizing ingredients have been used to remove unpleasant odors such as bad breath. Among them, those using plant extracts and oxidoreductases as deodorizing components are known to have a large deodorizing effect (Japanese Patent Laid-Open Nos. 63-309269, 9-38183, and 10). No. -212221 and JP-A No. 2004-321077). However, when these deodorizing ingredients are applied to chewing gum, for example, a small amount cannot exert a sufficient effect, so it is necessary to add a large amount. However, adding a large amount affects the taste and is disadvantageous in terms of cost. . In addition, these deodorizing components also have a problem that the deodorizing effect is inferior under acidic conditions (for example, a state where saliva pH becomes 5 or less by chewing a sour food).

Summary of the Invention

  Surprisingly, the present inventors have found that when the distillation residue after steam distillation of peppermint or spearmint and the oxidoreductase are added to chewing gum, the amount of peppermint or spearmint distillation residue is small. It was also found that chewing gum having a very high deodorizing effect can be obtained even under acidic conditions (Test Examples 1 and 2). The present invention is based on this finding.

  An object of the present invention is to provide a deodorant composition that exhibits a high deodorizing effect even in a small amount or under acidic conditions when applied to food (especially chewing gum). .

  According to the present invention, there is provided a deodorant composition (hereinafter referred to as “deodorant composition according to the present invention”) comprising an extraction residue after extraction of a fragrance component of mint and an oxidoreductase.

  According to the present invention, there is also provided a chewing gum comprising the deodorant composition according to the present invention (hereinafter referred to as “chewing gum according to the present invention”).

  The deodorant composition according to the present invention is advantageous in terms of taste and cost compared to conventional deodorant components because a high deodorant effect can be obtained even if the amount of the extraction residue after extraction of the mint perfume component is small. Moreover, since the deodorizing effect can be sufficiently exerted even under acidic conditions, it is advantageous in that it can be used for foods having a sour taste such as fruit taste. The deodorant composition according to the present invention is advantageous in that it is preferable for application to chewing gum because a high deodorizing effect can be obtained even in a small amount or under acidic conditions as compared with conventional deodorant components. is there.

  In addition, the deodorant composition according to the present invention and the chewing gum according to the present invention are advantageous in that the cost can be reduced by effectively utilizing the extraction residue after the extraction of the fragrance component of mint.

Detailed Description of the Invention

Deodorant Composition The deodorant composition according to the present invention is a composition comprising an extraction residue after extraction of the fragrance component of mint and an oxidoreductase.

  In the present specification, “extraction residue after extraction of flavor components of mint” means the remaining mint extracted from the flavor components of mint. The “extraction residue after extracting the fragrance component of mint” can be prepared from the remaining mint after the mint is subjected to the fragrance component extraction step and the fragrance component is extracted. The remaining mint after extracting the fragrance component may be used as it is, or may be used after drying. The dried mint may be further pulverized for use.

  Mint means a perennial plant belonging to the family Labiatae, preferably peppermint or spearmint.

  Mint can be used in the extraction of perfume ingredients in the raw state, or the dried product, all or a portion such as a leaf or stem.

  Examples of the method for extracting the fragrance component include known methods such as distillation and solvent extraction. Examples of the distillation include steam distillation and vacuum distillation. Examples of the solvent extraction include extraction with a polar solvent (water, ethanol, etc.) and a nonpolar solvent (fatty acid glyceride, hexane, acetone, etc.), preferably extraction with a nonpolar solvent.

  In the present invention, the “oxidoreductase” is not particularly limited as long as it meets the object of the present invention, but is preferably polyphenol oxidase, and more preferably laccase.

  A commercially available oxidoreductase may be used, or a oxidoreductase produced according to a known method may be used.

  When it is desired to maintain the deodorizing effect for a long period of time, the oxidoreductase can be used by being encapsulated in a capsule formed of a coating such as gelatin.

  The capsule is not particularly limited as long as it does not interfere with the enzymatic reaction by opening the encapsulated enzyme when the deodorant composition of the present application is used, and can be appropriately selected according to the usage form. For example, what was manufactured according to a well-known method (Unexamined-Japanese-Patent No. 2004-321077 etc.) can be used.

  In the deodorant composition according to the present invention, the content of the extraction residue after extraction of the mint fragrance component in the total amount of the composition can be 0.002 to 2.0% by weight as a dry weight, which has an influence on the taste. When considered, it is preferably 0.01 to 0.5% by weight.

  In the deodorant composition according to the present invention, the content of the oxidoreductase is preferably about 3 to 30% with respect to the dry weight of the extraction residue after the extraction of the mint flavor. For example, in the case of laccase, it is about 3000 to 40000 POU with respect to 1 g of the dried extract residue after extraction of the mint fragrance component. The 1 POU unit means that when 4-aminoantipyrine and phenol act at pH 4.5 and 30 ° C., the absorbance at 505 nm of the quinoneimine dye produced by the oxidative condensation reaction catalyzed by laccase is increased by 0.1 for the first minute of the reaction. It is the amount of enzyme necessary to make it.

  According to a preferred embodiment of the present invention, after steam distillation of peppermint or spearmint leaves and stems, the leaves and stems are removed and dried to a water content of about 0.5% by weight, and then the dried bodies are milled. It is possible to obtain a deodorant composition comprising “pulverized mint extract residue” obtained by pulverization and laccase encapsulated in a capsule formed of a film such as gelatin.

  The deodorant composition according to the present invention can be preferably used for deodorization in the oral cavity.

Chewing gum The deodorant composition according to the present invention can be applied to toothpastes, troches, soaps, etc., but the deodorant composition according to the present invention is a small amount compared to conventional deodorant ingredients when applied to chewing gum. Can also be sufficiently effective. Moreover, the deodorant composition by this invention can fully exhibit an effect even if it is acidic conditions. Therefore, it is particularly preferable to apply to chewing gum.

  In the present invention, chewing gum refers to a confectionery produced by adding sugar, flavoring, etc. to a gum base as required, as defined in the fair competition rules regarding the display of chewing gum in Japan.

  In the present invention, “chewing gum” is used to mean plate-shaped gum, sugar-coated gum, bubble gum and the like.

  The chewing gum according to the present invention can be produced by appropriately adopting a conventionally used method. For example, it is produced by a general method such as adding a deodorant composition according to the present invention, sugar or sugar alcohol, acid, fragrance, etc. to a gum base, mixing and lumping, rolling, cutting and molding. can do.

  The final form of the chewing gum according to the present invention is not particularly limited, and it may be a plate-like gum, a granular gum or a tablet-formed gum, and may be subjected to a treatment such as sugar coating after molding.

  The chewing gum according to the present invention can use a gum base, sweeteners such as sugar and sugar alcohol, acids, and other raw materials commonly used in the production of chewing gum. Hereinafter, each component will be described.

[Gum base]
The gum base that can be added to the chewing gum according to the present invention can be appropriately selected depending on the purpose, and includes, for example, vegetable resins, thermosoftening resins, rubbers, natural waxes, fats and oils, fillers, and emulsifiers, and combinations thereof. It can be selected appropriately.

  Examples of plant resins include chicle, gelton, and solver.

  Examples of the thermosoftening resin and rubber include vinyl acetate resin, ester gum, polyisobutylene, and the like.

  Examples of natural waxes and fats include rice wax, candelilla wax, carnauba wax, ordinary vegetable oils, and hydrogenated hydrogenated oils thereof.

  Examples of the filler include talc and calcium carbonate.

  Examples of emulsifiers include glycerin esters, sorbitan esters, sugar esters, and the like of fatty acids.

[sweetener]
Sweeteners such as sugar and sugar alcohol can be added to the chewing gum according to the present invention.

  The sugar that can be added to the chewing gum according to the present invention can be appropriately selected depending on the purpose, and examples thereof include monosaccharides, disaccharides, and starch syrup. Examples of monosaccharides include glucose (glucose), galactose, mannose, ribose, arabinose, xylose, and rhamnose. Examples of the disaccharide include sucrose (sucrose), maltose, lactose, isomaltose, cellobiose, gentiobiose, palatinose and the like. Examples of starch syrup include acid saccharified starch syrup and reduced starch syrup. Sugars that can be added to the chewing gum according to the invention also include crystalline 1-kestose.

  The sugar alcohol that can be added to the chewing gum according to the present invention can be appropriately selected depending on the purpose, and examples thereof include sorbitol, xylitol, mannitol, erythritol, maltitol, and reduced palatinose.

  Sweeteners that can be added to the chewing gum according to the present invention include, in addition to the above, aspartame, acesulfame potassium, stevioside, glycyrrhizin, sodium saccharin, sucralose, thaumatin and the like.

[acid]
An acid can be added to the chewing gum according to the present invention as a sour agent, a strengthening agent, an antioxidant or the like.

  The acid can be appropriately selected according to the purpose. Examples thereof include various organic acids such as citric acid, malic acid, tartaric acid, fumaric acid, acetic acid, lactic acid and gluconic acid, ascorbic acid and the like. In the chewing gum according to the present invention, the content of the acid in the production raw material can be 0 to 10% by weight, and preferably 0 to 5% by weight when the influence on the taste is considered. The chewing gum according to the present invention is advantageous in that the deodorizing effect is maintained even when the acid content is 0 to 10% by weight.

[Other ingredients]
The chewing gum according to the present invention may further contain a softener, a colorant, and a fragrance, if necessary, alone or in appropriate combination.

  The softening agent can be appropriately selected depending on the purpose, and examples thereof include polyhydric alcohols and pastes. Examples of the polyhydric alcohol include glycerin and propylene glycol. Examples of the paste include gum arabic solution and gelatin solution.

  The colorant can be appropriately selected depending on the purpose, and examples thereof include natural colorants and synthetic colorants.

  A fragrance | flavor can be suitably selected according to the objective, For example, a natural fragrance | flavor, a synthetic | combination fragrance | flavor, a mixing | blending fragrance | flavor, etc. are mentioned.

  According to this invention, the deodorizing composition by this invention whose pH is 5 or less is provided.

  ADVANTAGE OF THE INVENTION According to this invention, the manufacturing method of the deodorizing composition which comprises the extraction residue after fragrance | flavor component extraction of mint, and an oxidoreductase is provided.

  ADVANTAGE OF THE INVENTION According to this invention, the bad breath suppression agent which comprises the extraction residue after fragrance | flavor component extraction of mint, and an oxidoreductase is provided.

  According to the present invention, there is provided use of a composition comprising an extraction residue after extraction of a mint fragrance component and an oxidoreductase for producing a halitosis inhibitor.

  According to the present invention, there is provided a method for suppressing bad breath comprising a step of ingesting a mammal including a human with a composition comprising an extraction residue after extraction of a fragrance component of an effective amount of mint and an oxidoreductase. Provided.

  Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

Preparation of Extraction Residue After Extraction of Flavor Components of Mint Peppermint or spearmint (hereinafter referred to as “mint”) leaves and stems were placed in a steam distillation apparatus, and steam was passed through the mint for 3 hours. The component evaporated at this time was recovered as a mint flavor component. After completion of distillation, the mint leaves and stems are taken out, dried to a water content of about 0.5% by weight, and then pulverized with a mill to obtain the extraction residue (peppermint or spearmint extraction residue). Powder).

Preparation of Peppermint Solvent Extract 10 g of peppermint leaves and 90 g of medium chain fatty acid glyceride (Nisshin Oillio Group Co., Ltd.) were mixed and heated at 60 ° C. for 1 hour. The mixture was centrifuged at 3000 rpm for 5 minutes, and the supernatant was collected and used as a peppermint solvent extract.

Preparation of laccase-encapsulated capsules Laccase-encapsulated capsules were prepared by the following method. The laccase was suspended in hardened palm oil at 160 ° C. for 30 minutes at 40 ° C. with a stirrer to prepare a filling solution. Further, gelatin and D-sorbitol were mixed to prepare a material for forming a capsule outer coating solution. Next, in the capsule manufacturing machine, each of the filling liquid from the innermost nozzle of the triple nozzle, the hardened palm oil as the material for forming the capsule inner film from the intermediate nozzle, and the material for forming the capsule outer film liquid from the outer nozzle By simultaneously discharging from the tip of the hole, a triple-structured laccase-encapsulating capsule (laccase content 5 wt%) in which laccase was enclosed in the innermost shell was prepared. The composition and the amount of capsules are as shown in Table 1.

Preparation of chewing gum A 3.5 g piece of chewing gum was prepared by a conventional method with the formulation shown in Tables 2 and 3 (the unit of the numerical values in the table is% by weight).

Test Example 1: Deodorant effect measurement test of extraction residue after extraction of flavor component of peppermint (1) Preparation of test liquid Each chewing gum (3.5 g) of Examples 1 and 2 and Comparative Examples 1 to 4 was each mortar 10 ml of 0.05 M phosphate buffer pH 7.0 at 37 ° C. was added and thoroughly mixed with a pestle for 10 minutes. A liquid obtained by mixing is used as a test liquid.

(2) Measurement of pH For the test solutions of Examples 1 and 2 and Comparative Examples 1 to 4 obtained in (1), the pH was measured at 25 ° C. using a normal glass electrode, and no citric acid was added. Both Example 1 and Comparative Examples 1 and 2 were near pH 7, and Example 2 and Comparative Examples 3 and 4 to which citric acid was added were both near pH 4 (Table 4).

(3) Deodorizing effect measurement test 5 ml of each of the test solutions of Examples 1 and 2 and Comparative Examples 1 to 4 obtained in (1) were placed in 30 ml vials, and 500 ng each of methyl mercaptan was added thereto. Sealed and allowed to react at 37 ° C. for 10 minutes. After the reaction, 5 ml of gas was extracted from each vial head space, and the amount of methyl mercaptan was measured by gas chromatography. As a control, the amount of methyl mercaptan contained in 5 ml of headspace gas after adding 500 ng of methyl mercaptan to 5 ml of the phosphate buffer placed in a 30 ml vial and reacting at 37 ° C. for 10 minutes, Table 4 shows the remaining amount of methyl mercaptan in each sample when the measured amount is 100.

  In the chewing gum that contains the extraction residue after the extraction of the flavor components of peppermint and laccase, the flavor of peppermint compared to the chewing gum that contains the rosemary extract and laccase, which is known to have a deodorizing effect in combination with laccase It was confirmed that the deodorizing effect was remarkably high even when the amount of the extraction residue after the component extraction was small, and that the deodorizing effect was high even under acidic conditions. Here, in the chewing gum blended with the peppermint solvent extract and laccase, no deodorizing effect was observed under acidic conditions even when blended with a peppermint solvent extract twice as much as the extraction residue. From this, it was shown that the deodorant component exists in the extraction residue after extraction of the flavor component of peppermint.

  In addition, when the polyphenol density | concentration was measured about the peppermint leaf and rosemary extract after steam distillation, they were 3.4 weight% and 2.6 weight%, respectively. From this, it became clear that the difference of the deodorizing effect more than the concentration difference of polyphenol was recognized.

Test Example 2: Deodorant effect measurement test of extracted residue after extraction of spearmint fragrance component (1) Preparation of test solution Each chewing gum (3.5 g) of Examples 3 and 4 and Comparative Examples 5 and 6 was each mortar 10 ml of 0.05 M phosphate buffer pH 7.0 at 37 ° C. was added and thoroughly mixed with a pestle for 10 minutes. A liquid obtained by mixing is used as a test liquid.

(2) Measurement of pH For the test solutions of Examples 3 and 4 and Comparative Examples 5 and 6 obtained in (1), the pH was measured at 25 ° C. using a normal glass electrode, and no citric acid was added. Both Example 3 and Comparative Example 5 were in the vicinity of pH 7, and both Example 4 and Comparative Example 6 to which citric acid was added were in the vicinity of pH 4 (Table 5).

(3) Deodorizing effect measurement test 5 ml of each of the test solutions of Examples 3 and 4 and Comparative Examples 5 and 6 obtained in (1) were placed in 30 ml vials, and 500 ng of methyl mercaptan was added thereto. Sealed and reacted at 37 ° C. for 10 minutes. After the reaction, 5 ml of gas was extracted from each vial head space, and the amount of methyl mercaptan was measured by gas chromatography. As a control, the amount of methyl mercaptan contained in 5 ml of headspace gas after adding 500 ng of methyl mercaptan to 5 ml of the phosphate buffer placed in a 30 ml vial and reacting at 37 ° C. for 10 minutes, Table 5 shows the remaining amount of methyl mercaptan in each sample when the measured amount is 100.

  In chewing gum that contains extraction residue after spearmint fragrance component extraction and laccase, extraction residue after extraction of spearmint fragrance component, compared to chewing gum that contains navel dry peel and laccase, which is known for its deodorizing effect It was confirmed that the deodorizing effect was remarkably high even with a small amount of blending, and that the deodorizing effect was high even under acidic conditions.

Claims (8)

  A deodorant composition comprising an extraction residue after extraction of a mint fragrance component and an oxidoreductase.   The deodorizing composition according to claim 1, wherein the mint is peppermint or spearmint.   The deodorant composition according to claim 1, wherein the perfume component extraction is performed by distillation.   Deodorant composition as described in any one of Claims 1-3 whose content in the composition whole quantity of the extraction residue after the fragrance | flavor component extraction of mint is 0.002-2.0 weight% as dry weight. object.   The deodorizing composition according to any one of claims 1 to 4, wherein the oxidoreductase is polyphenol oxidase.   The deodorizing composition according to any one of claims 1 to 5, wherein the oxidoreductase is encapsulated in a capsule.   The deodorizing composition according to any one of claims 1 to 6, wherein the content of the oxidoreductase is 3 to 30% based on the dry weight of the extraction residue after extraction of the mint flavoring component.   A chewing gum comprising the deodorant composition according to any one of claims 1 to 7.