JPS6429305A - Separation of biomembrane - Google Patents
Separation of biomembraneInfo
- Publication number
- JPS6429305A JPS6429305A JP62185064A JP18506487A JPS6429305A JP S6429305 A JPS6429305 A JP S6429305A JP 62185064 A JP62185064 A JP 62185064A JP 18506487 A JP18506487 A JP 18506487A JP S6429305 A JPS6429305 A JP S6429305A
- Authority
- JP
- Japan
- Prior art keywords
- glucomannan
- biomembrane
- column
- spherical particles
- give
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
PURPOSE:To efficiently separate and purify a biomembrane without deforming spherical particles even under pressure, by passing a solution containing the biomembrane through a column packed with spherical particles of crosslinked glucomannan. CONSTITUTION:Glucomannan is swollen with formamide, treated with acetic acid, etc., in the presence of an esterifying catalyst to give a glucomannan ester, which is dissolved in a solvent such as dichloromethane. In the operation, a simultaneous addition of 100-500wt.% based on the glucomannan of a diluent (e.g. THF) can provide a gel having >=1,000,000 exclusion limit mol.wt. Then the solution is stirred in an aqueous medium to give spherical particles having 1-500mum particle diameter. The particles are packed into a column, a solution containing a biomembrane (e.g. natural liposome, synthetic bimolecular membrane or multi-layer liposome) is passed through the column and separated.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62185064A JPS6429305A (en) | 1987-07-23 | 1987-07-23 | Separation of biomembrane |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62185064A JPS6429305A (en) | 1987-07-23 | 1987-07-23 | Separation of biomembrane |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6429305A true JPS6429305A (en) | 1989-01-31 |
Family
ID=16164174
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62185064A Pending JPS6429305A (en) | 1987-07-23 | 1987-07-23 | Separation of biomembrane |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6429305A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02212763A (en) * | 1989-02-13 | 1990-08-23 | Tetsuo Yamamoto | Packing agent for affinity chromatography |
CN103194008A (en) * | 2013-03-26 | 2013-07-10 | 中国科学院过程工程研究所 | Uniform-sized konjak gulcomannan microspheres and preparation method thereof |
-
1987
- 1987-07-23 JP JP62185064A patent/JPS6429305A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02212763A (en) * | 1989-02-13 | 1990-08-23 | Tetsuo Yamamoto | Packing agent for affinity chromatography |
CN103194008A (en) * | 2013-03-26 | 2013-07-10 | 中国科学院过程工程研究所 | Uniform-sized konjak gulcomannan microspheres and preparation method thereof |
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