JPS6328413B2 - - Google Patents
Info
- Publication number
- JPS6328413B2 JPS6328413B2 JP57122016A JP12201682A JPS6328413B2 JP S6328413 B2 JPS6328413 B2 JP S6328413B2 JP 57122016 A JP57122016 A JP 57122016A JP 12201682 A JP12201682 A JP 12201682A JP S6328413 B2 JPS6328413 B2 JP S6328413B2
- Authority
- JP
- Japan
- Prior art keywords
- molecular weight
- gel filtration
- extract
- substance
- yakifutake
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000126 substance Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 21
- 238000002523 gelfiltration Methods 0.000 claims description 16
- -1 nitrogen-containing polysaccharide Chemical class 0.000 claims description 11
- 230000000259 anti-tumor effect Effects 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 10
- 229920001282 polysaccharide Polymers 0.000 claims description 9
- 239000005017 polysaccharide Substances 0.000 claims description 9
- 239000003125 aqueous solvent Substances 0.000 claims description 6
- 239000002504 physiological saline solution Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 description 14
- 206010003445 Ascites Diseases 0.000 description 8
- 208000006268 Sarcoma 180 Diseases 0.000 description 7
- 230000003308 immunostimulating effect Effects 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 210000004698 lymphocyte Anatomy 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000007912 intraperitoneal administration Methods 0.000 description 4
- 239000012134 supernatant fraction Substances 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000001223 reverse osmosis Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 239000012506 Sephacryl® Substances 0.000 description 2
- 241001676647 Trametes pubescens Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000003217 anti-cancerogenic effect Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000000921 elemental analysis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000005934 immune activation Effects 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000002235 sarcomere Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000195888 Physcomitrella Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000003855 balanced salt solution Substances 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 102000034238 globular proteins Human genes 0.000 description 1
- 108091005896 globular proteins Proteins 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004705 lumbosacral region Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 230000003393 splenic effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57122016A JPS5913729A (ja) | 1982-07-15 | 1982-07-15 | 含窒素多糖体の製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57122016A JPS5913729A (ja) | 1982-07-15 | 1982-07-15 | 含窒素多糖体の製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5913729A JPS5913729A (ja) | 1984-01-24 |
| JPS6328413B2 true JPS6328413B2 (cs) | 1988-06-08 |
Family
ID=14825484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57122016A Granted JPS5913729A (ja) | 1982-07-15 | 1982-07-15 | 含窒素多糖体の製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5913729A (cs) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5244380A (en) * | 1975-10-03 | 1977-04-07 | Toyota Motor Corp | Dual system master cylinder for a motor vehicle |
| JPS536412A (en) * | 1976-07-07 | 1978-01-20 | Kureha Chem Ind Co Ltd | Preparation of n-containing polysaccharides |
-
1982
- 1982-07-15 JP JP57122016A patent/JPS5913729A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5913729A (ja) | 1984-01-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4202885A (en) | Process for the preparation of anti-transplanted sarcoma 180 tumorigenic substances | |
| CS219880B2 (en) | Method of isolation of the polysaccharide | |
| KR20020063618A (ko) | 버섯으로부터의 생리 활성 물질 ｅｅｍ-ｓ, 그의 제조방법 및 의약 | |
| JPS62209101A (ja) | エキナセアプルプレアまたはエキナセアアングステイフオリアの細胞培養物からの免疫刺激作用性ポリサツカライド、その製造方法及びこれを含む製剤 | |
| DE2441454A1 (de) | Verfahren zur herstellung eines heilmittels fuer leukaemie | |
| NL8204323A (nl) | Fysiologisch actieve stof; werkwijze voor het bereiden daarvan. | |
| CA1072033A (en) | Method of producing nitrogen-containing polysaccharides | |
| CN112725399A (zh) | 一种香菇寡肽的制备方法及用途 | |
| DE3782430T2 (de) | Verwendung eines trypsininhibitors des kunitztyps aus sojabohnen und seiner derivate zur herstellung von medikamenten und denselben enthaltendes agens. | |
| CA2035948A1 (en) | Polysaccharides with immunomodulating properties from astragalus membranaceus and pharmaceutical compositions containing them | |
| US6120772A (en) | Oral drugs for treating AIDS patients | |
| CN105796587B (zh) | 竹茹多糖在免疫调节、抗肿瘤中的应用 | |
| US4209507A (en) | Novel anti-tumor substance and preparation thereof | |
| JPS6328413B2 (cs) | ||
| JPH04300898A (ja) | 新規糖タンパク複合体およびその製造方法 | |
| EP0067000A2 (en) | Production of a nitrogen-containing polysaccharide having antitumour activity | |
| JP4010519B2 (ja) | エイズ治療用経口投与剤 | |
| JP4179901B2 (ja) | 細胞付着及び増殖促進剤 | |
| JPS6058925A (ja) | 抗腫瘍活性物質 | |
| IL25759A (en) | A method of producing an active ingredient against cancer | |
| KR810001499B1 (ko) | 질소를 함유하는 다당류의 제조방법 | |
| JPH0725780A (ja) | 抗菌・抗癌剤 | |
| JPH06183981A (ja) | クロレラ菌体外粘質多糖体を含有する抗腫瘍剤 | |
| JPH0156075B2 (cs) | ||
| JPH0571232B2 (cs) |