JPS63216813A - Composition for skin - Google Patents

Abstract

PURPOSE:To obtain a composition for the skin capable of enhancing hydrolyzing function of sphingomyelin to ceramide and imparting excellent humectant function, by blending an extract ingredient containing aphingomyelinase derived from bodies. CONSTITUTION:A composition of the skin obtained by homogenizing tissues, such as brain or liver of a higher animal, in a 50mM acetic acid buffer solution at pH 4.7, carrying out fractional centrifugation to provide a supernatant at 600Xg and mitochondrial lysosome fraction precipitating at 9,000Xg, subjecting the resultant fraction to ultrasonic treatment, ultracentrifuging the obtained fraction (at 100,000Xg), fractionating the resultant supernatant with ammonium sulfate, dissolving the formed precipitates in purified water, dialyzing the solution against purified water to prepare a sphinglomyelinase solution, preferably enclosing the resultant enzymic solution in liposome and blending the enclosed solution therein. Thereby the function of sphingomyelinase deteriorated by aging on the epidermis is activated.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a skin composition having a moisturizing function and containing an extracted component containing sphingomyelinase.

[Conventional technology]

Conventionally, symptoms and skin diseases caused by skin dryness, such as skin cracks, chapped skin, ichthyosis, and senile xeroderma, have been a problem.

Cracks and chapped skin are caused by transepidermal water loss caused by dry air.
1oss.

This occurs as a result of an increase in T, -L,), the loss of sebum due to the use of detergents, a decline in the protective function of the sebum film, and a loss of moisture in the stratum corneum of the epidermis.

Ichthyosis is a type of keratosis 141, which is a skin disease in which the skin becomes rough and dry, causing the skin surface to appear cracked and scaly, and the symptoms are especially worse during the dry winter season.

Furthermore, as we age, the sebum secretion ability declines, and the protective function of the sebum film also declines, resulting in a decrease in the amount of water in the stratum corneum of the epidermis, which can lead to skin diseases. Senile xeroderma is one example.

Thus, these symptoms and skin diseases are caused by the presence of water combined with the constituent components of the stratum corneum at low temperatures.

This occurs when the epidermis becomes dry because it is lost due to low humidity or aging. Therefore, in order to deal with such symptoms, it is necessary to strengthen the deteriorated skin protective function, prevent a decrease in the water content of the stratum corneum, and maintain normal skin function.

So far, squalane, lanolin, and jojoba oil.

Emollients such as fatty acid esters, sl/lium lactate, glycerin, sodium pyrrolidone carboxylate,
Skin preparations and cosmetics containing moisturizing agents such as propylene glycol, soluble collagen, and hyaluronic acid have been used. Recently, a technique has been reported in which polysaccharides and lipids derived from tissues such as the brain, liver, and lungs of mammals are used as moisturizing agents (Japanese Patent Application Laid-Open No. 60-163806, JP-A No. 60-163888).

(Patent application No. 61-167260).

[Problem that the invention seeks to solve]

However, although these emollients prevent the evaporation of water on the skin surface, this effect is physical and temporary. On the other hand, the above-mentioned humectants serve the function of supplying moisture to the stratum corneum on the skin surface, and again have the disadvantage that they only provide a temporary physical effect and are greatly influenced by environmental conditions.

Furthermore, treatments for skin diseases such as ichthyosis and senile xeroderma have not been established, and at present, antihistamines are taken orally and steroid ointments are locally administered.

[Means for solving problems]

In Japanese Patent Application No. 61-198104, we stated that sphingolipids derived from epidermal cells decrease with age, and are particularly low in patients with senile xeroderma, and are also low in rough skin. A series of recent techniques for incorporating polysaccharides and lipids derived from mammalian tissues can be considered to be an attempt to compensate for the decrease in epidermal lipids caused by aging or epidermal damage.

In this study, we revealed that in the sphingolipid metabolic pathway, sphingomyelinase activity, which hydrolyzes sphingomyelin to produce ceramide, decreases with age (Figure 1). That is, 8 to 46
Week-old hairless rat skin was treated with dispase to peel off the epidermis, homogenized using 50 RIM acetate buffer pH 4,7, and centrifuged at 600×g for 20 minutes, and the supernatant was used as a sample. 1 ml of this sample was added to 1 ml of pig brain extract prepared using 50 mM acetate buffer (pH 4,7) with Triton
(W/V)%) and incubate at 37°C for 2 hours, followed by chloroform-methanol (2:1
) to extract sphingomyelin, and IIP
Quantification was performed by TLC.

A control sample in which only pig brain extract was treated in the same manner without adding any sample was used to determine the amount of sphingomyelin reduction, and the amount of sphingomyelin reduction per 1 hour of total protein determined by the Lowry method was determined using sphingomyelinase. It was made active.

Ceramide forms a water-containing liquid crystal state in the stratum corneum of the epidermis, and is thought to be involved in improving skin occlusiveness and water retention. It is suggested that this may be a contributing factor to skin roughness and skin diseases caused by a decrease in the moisture content of the layer.

Focusing on this point, we considered incorporating sphingomyelinase into skin compositions such as external preparations or cosmetics.

Mainly, tissues such as the brain, liver, and lungs of higher animals are collected in a 50 m
Homogenize in M acetate buffer p) 1.4.7 and perform differential centrifugation to obtain the supernatant at 600×g and the mitochondria-lysosome fraction precipitated at 9,000×g.

This was subjected to ultrasonication and ultracentrifugation (100,000X
g) A precipitate obtained by ammonium sulfate fractionation (40 to 50% saturation) from the supernatant was dissolved in purified water and dialyzed against purified water to prepare a sphingomyelinase solution. It is more preferable to add this enzyme solution encapsulated in ribosomes, since the stability of the enzyme can be improved.

[Examples and effects]

Further, the present invention will be explained in detail with reference to Examples.

(Example, 1) Ointment (1) Squalane 20.0 (wt%) (2) Vaseline 50.0
(31P, 0.E, (2) Sorbitan monostearate 6.0 (4) Beeswax 3.0 (5)
Sphingomyelinase solution 3,0 (6) Glycerin 1000 (7) Prevention lI punishment
0.2 (8) Purified water 7.8
(1) to (4) are mixed and heated to 75 to 80°C, a mixed and dissolved mixture of (6) to (8) is added thereto, and the mixture is cooled while stirring. Add (5) at 35° C. and further cool to room temperature.

(Example, 2) Cream (1) Stearic acid 2.0 (wt%) (2) Stearyl alcohol 1.0 (3
) Reduced lanolin 1.8 (4)
Squalane 1000 (5) Octyldodecanol 6.0 (61P, 0.E, (25)
Cetyl ether 3.0 (7) Glyceryl monostearate 2.0 (8) Phosphatidylethanolamine 0.6 (9) Sphingomyelinase solution 0.6 Ql
Preservative 0.2αυ glycerin
5.0＠Fragrance
0.301 Purified water 67.5 (
Mix 1) to (8) and heat to 80 to 85℃, add α
[.

A mixed solution of αl) and Q10 is added and emulsified.

Cool while stirring, add (91, (115) at 35°C, and further cool to room temperature.

(Example, 3) Emulsion (11P, 0.E, addition (50) hydrogenated castor oil 1.O
(% by weight) (2) Coconut oil fatty acid monoglyceride 1.
0(3) Purified soybean lecithin 0.3(4)
Oleic acid triglyceride 7.5 (5) Sphingomyelinase solution 0.3 (6) Glycerin
2.5 (7) Fragrance
0.2(8) Preservative 0
．． 2 (9) Purified water 87.0 (1
) to (4) were mixed and heated to 80-85℃, and (6
).

(8), (Mix and dissolve 91 and gradually add it, emulsify and dilute the emulsion. Cool while stirring,
Add (5) and (7) at 5°C, and further cool to room temperature.

Next, the following experiments were conducted for Examples to examine the effects of the present invention. In each Example, the total amount was adjusted to 100.0% by weight with purified water without adding sphingomyelinase solution, and was used as Comparative Example 1.2.3.

(1) Effect on skin surface moisture content Examples 1 to 3 were applied to the inside of the right forearm of five female panelists, and Comparative Examples 1 to 3 were applied to the inside of the left forearm at different sites for one week, respectively. Orally measure the skin surface moisture content at each application site using an impedance meter (1, B, S Company Model 1B-
355) at 20°C and 50% humidity, and the average value of 5 people is shown in Figure 2.

(2) Effect on skin T, -1, Five female panelists continued to apply the Examples and Comparative Examples in the same manner as in (1), and then orally administered T, -1 at the application site.
1, was measured using the cobalt chloride paper method, and the average value of 5 people was calculated as the third
Shown in the figure. In the cobalt chloride paper method, anhydrous cobalt chloride is adsorbed on filter paper and applied to the skin, and the change in color tone is measured using a high-speed spectrophotometer CM-1200 manufactured by Murakami Color Research Institute (20°C, humidity 50%). .

(3) Effect on improvement of rough skin conditions During the winter, 20 female panelists who complained of rough skin symptoms were asked to use each sample on the same area for one week in a blind manner using the Examples and Comparative Examples. In addition to performing a sensory evaluation, a replica of the application site of each sample was taken to observe the skin condition. Table 1 shows the results.
Shown below.

Table 1. Effect on improving rough skin condition The numbers indicate the number of applicable panelists.

From FIGS. 2 and 3, it is clear that the addition of the sphingomyelinase solution suppresses T, W, and increases the amount of moisture on the skin surface. Furthermore, as shown in Table 1, when using Examples, none of the panelists answered that rough skin was not improved, and most of them answered that it was improved. On the other hand, when using the comparative example, almost no panelists answered that their rough skin had improved. The results of this sensory evaluation corresponded well with the results of observation of the skin condition, and an improvement was observed in all the panelists in the Examples, but almost no improvement was observed in the Comparative Examples.

As described above, according to the present invention, it was possible to obtain a skin composition that activates the degraded function of sphingomyelinase in the epidermis to decompose sphingomyelin into ceramide and provides an excellent moisturizing function.

[Brief explanation of the drawing]

Figure 1 is a diagram showing age-related changes in sphingomyelinase activity, Figure 2 is a diagram showing the effect of the present invention on skin surface moisture content, and Figure 3 is a diagram showing changes in sphingomyelinase activity due to aging. FIG. 3 is a diagram showing the influence of the present invention. Patent applicant Noevir Co., Ltd. 1st Keifai F3 Kei

Claims (2)

[Claims] (1) A skin composition containing an extracted component containing biologically derived sphingomyelinase. (2) Extract components containing sphingomyelinase,
The skin composition according to claim 1, which is incorporated into ribosomes.