JPS63148977A - Air-permeable material for cultivating germs - Google Patents
Air-permeable material for cultivating germsInfo
- Publication number
- JPS63148977A JPS63148977A JP29710886A JP29710886A JPS63148977A JP S63148977 A JPS63148977 A JP S63148977A JP 29710886 A JP29710886 A JP 29710886A JP 29710886 A JP29710886 A JP 29710886A JP S63148977 A JPS63148977 A JP S63148977A
- Authority
- JP
- Japan
- Prior art keywords
- air permeability
- bacteria
- paper
- air
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000463 material Substances 0.000 title claims abstract description 35
- 244000052616 bacterial pathogen Species 0.000 title 1
- 230000035699 permeability Effects 0.000 claims abstract description 15
- 239000005871 repellent Substances 0.000 claims abstract description 7
- 239000000835 fiber Substances 0.000 claims abstract description 4
- 229920000098 polyolefin Polymers 0.000 claims abstract description 3
- 241000233866 Fungi Species 0.000 claims description 23
- 238000012258 culturing Methods 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 230000002940 repellent Effects 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- -1 polyethylene Polymers 0.000 description 4
- 238000004513 sizing Methods 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 229910021538 borax Inorganic materials 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000005470 impregnation Methods 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000013055 pulp slurry Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000004328 sodium tetraborate Substances 0.000 description 2
- 235000010339 sodium tetraborate Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- DTCCVIYSGXONHU-CJHDCQNGSA-N (z)-2-(2-phenylethenyl)but-2-enedioic acid Chemical compound OC(=O)\C=C(C(O)=O)\C=CC1=CC=CC=C1 DTCCVIYSGXONHU-CJHDCQNGSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 1
- 229910000272 alkali metal oxide Inorganic materials 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- VBIXEXWLHSRNKB-UHFFFAOYSA-N ammonium oxalate Chemical compound [NH4+].[NH4+].[O-]C(=O)C([O-])=O VBIXEXWLHSRNKB-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000012744 reinforcing agent Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は各種菌類特に糸状菌やバクテリアの培養に用い
る好適な通気性材料に関するものである。DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a breathable material suitable for culturing various fungi, particularly filamentous fungi and bacteria.
〈従来の技術〉
最近、プラスチック製のビンあるいは袋内で菌類を培養
する方法が広く行なわれるようになってきた。<Prior Art> Recently, methods of culturing fungi in plastic bottles or bags have become widely used.
これらは、プラスチック製のビンあるいは袋内に大鋸屑
などの培地を入れ、これをオートクレーブ等で蒸気殺菌
した後、培地内に菌を植え付け、それを培養することに
よって行なわれる。These methods are carried out by placing a medium such as sawdust in a plastic bottle or bag, sterilizing it with steam in an autoclave, etc., and then inoculating bacteria into the medium and culturing it.
この場合、菌の培養に必要な空気を供給するためにビン
や袋に開口部を設けたり、あるいは容器本体の一部に種
々の方法で小孔を穿孔した多孔性プラスチックフィルム
を設けたりしている。In this case, an opening is provided in the bottle or bag to supply the air necessary for culturing the bacteria, or a porous plastic film with small holes perforated by various methods is provided as part of the container body. There is.
この開口部には通常綿栓を施すが、密封しすぎると空気
供給量が不足し、弱すぎるとアオカビ等の雑菌が侵入し
やすくなる等、非常に熟練を要し、また手間もかかるも
のであった。一方、多孔性フィルムを用いる場合には、
綿栓を用いる場合に比べてかなり良いが１通気性を保持
するため多数の小透孔を必要とするため、雑菌の侵入を
防止するには問題があるばかりでなく、コスト而でも高
価である欠点があった。This opening is usually sealed with a cotton plug, but if it is too tightly sealed, the air supply will be insufficient, and if it is too weak, bacteria such as green mold can easily enter, so it requires great skill and time. there were. On the other hand, when using a porous film,
Although it is considerably better than using cotton plugs, it requires a large number of small holes to maintain breathability, which not only poses a problem in preventing the invasion of bacteria, but is also expensive. There were drawbacks.
〈発明が解決しようとする問題点〉
本光明は、このような従来技術が有している通気性の保
持と雑菌の侵入防止というジレンマを克服し、充分な通
気性を保ちながら雑菌の侵入を防ぎ、しかも培地の水分
蒸発を適度に押え、糸状菌やバクテリアの生長の早い安
価な菌類の培養用通気性材料を提供することを目的とす
る。<Problems to be solved by the invention> This Komei overcomes the dilemma of maintaining air permeability and preventing the intrusion of bacteria, which the conventional technology has, and prevents the intrusion of bacteria while maintaining sufficient air permeability. To provide an inexpensive breathable material for culturing fungi that prevents the evaporation of water in a medium and allows filamentous fungi and bacteria to grow quickly.
く問題点を解決するための手段〉
本発明者は蒸気のような問題点を解決するため、ｐＨが
７〜１１、秤量が３００ｇ４以上で、かつ透気度がＪ
Ｉ Ｓ Ｐ８１１７に規定する空気透過度試験機で５〜
５０秒を有する膜体で構成した構造としたものである。Means for Solving the Problems〉 In order to solve the problems such as steam, the inventors of the present invention have determined that the pH is 7 to 11, the weight is 300g4 or more, and the air permeability is J
5~ with air permeability tester specified in ISP8117
It has a structure composed of a film body having a duration of 50 seconds.
一般に、菌類、例えばキノコ類のような糸状菌を培養す
る通気材料には、次の特性が要求される。In general, the following characteristics are required for ventilated materials for cultivating fungi, such as filamentous fungi such as mushrooms.
■ オートクレーブ等による蒸気殺菌時に、破損しない
こと。■ It should not be damaged during steam sterilization using an autoclave, etc.
■ 培養時に水との接触があっても破損したり、水を通
したりしないこと。■ Even if it comes into contact with water during culture, it will not be damaged or allow water to pass through.
■ 培養時に雑菌の侵入を防ぐと共に、培養に必要な空
気を充分供給できること。■ Prevent the invasion of bacteria during culturing, and be able to supply sufficient air necessary for culturing.
■ 培地の水分蒸発を適度におさえることができること
。■ Be able to moderately suppress water evaporation in the medium.
本発明に係る通気性材料は、これらの特性をすべてかね
そなえている。The breathable material according to the invention has all of these properties.
即ち、本発明に係る材料は、Ｊ ｌ ３ ｐ−８１１７
に規定されている方法により、透気度が５〜５０秒の範
囲を有し、雑菌が侵入することなく培養に必要な空気を
充分供給でき、しかも培地の水分蒸発を適度でおさえる
ことができる。透気度がこの範囲より小さくなると、雑
菌が侵入しやすくなり、培地の水分蒸発が過度となる。That is, the material according to the present invention is J l 3 p-8117
By using the method specified in , the air permeability is in the range of 5 to 50 seconds, it is possible to supply sufficient air necessary for culture without intrusion of bacteria, and it is possible to suppress moisture evaporation of the medium to a moderate level. . If the air permeability is lower than this range, bacteria will easily invade and the moisture in the medium will evaporate excessively.
一方この範囲より大きな透気度の場合は、培養に必要な
空気の供給ができず菌類の生育が抑制される。On the other hand, if the air permeability is greater than this range, the air necessary for culture cannot be supplied, and the growth of fungi will be suppressed.
更に本発明の通気性材料２は、そのｐｌ＋が７〜１１、
望ましくは７〜９である。このＩ）Ｈの範囲内では糸状
菌の育成を防止できるため、植菌時あるいは培養時に何
かの原因により通気性材料部分に糸状菌が付着しても、
糸状菌が成長して通気性材料の目を塞いだり、このよう
に通気性材料に付着して成長した糸状菌を伝わって１菌
が内部に侵入するようなことも起らない。Furthermore, the breathable material 2 of the present invention has a pl+ of 7 to 11,
Desirably it is 7-9. Since the growth of filamentous fungi can be prevented within the range of I)H, even if filamentous fungi adhere to the breathable material part for some reason during inoculation or culturing,
There is no possibility that filamentous fungi will grow and block the holes in the breathable material, or that one bacteria will infiltrate the interior through the filamentous fungi that have grown attached to the breathable material.
本発明の材料は、雑菌の侵入を防ぎ、必要な湿潤強度を
維持するために、３０ｆ１４以上、最も好ましくは３０
〜１２０９４秤堡が必要である。又、オートクレーブ等
による蒸気殺菌時あるいは培養中にＪ３こる恐れのある
水との接触時に破損したり、水が内部に侵入することを
防ぐためにＪＩＳＰ−８１２２で規定する方法で少なく
とも３０秒以上の耐水性を有することが望ましい。The material of the present invention is preferably 30 f14 or higher, most preferably 30
~12094 scale forts are required. In addition, in order to prevent breakage or water intrusion into the interior during steam sterilization using an autoclave, etc. or contact with water that may cause J3 damage during culture, it must be water resistant for at least 30 seconds using the method specified in JISP-8122. It is desirable to have gender.
本発明の上記通気性材料２を構成する膜体の材料として
は、撥水性滅菌紙、ポリオレフィンＩ維１００％紙をあ
げることができ、撥水性滅菌紙は培養容器１に対して接
着剤による貼付けによって取付け、またポリエチレン４
Ｉ維１００ｇ紙はヒートシールによって培養容器１に取
付けられる。Examples of the material of the membrane constituting the above-mentioned breathable material 2 of the present invention include water-repellent sterile paper and 100% polyolefin I fiber paper, and the water-repellent sterile paper is attached to the culture container 1 with an adhesive. Mounted by polyethylene 4
100 g of I-fiber paper is attached to the culture container 1 by heat sealing.
次に通気性材料２の製造方法として、撥水性滅菌紙の場
合を例に具体的に説明する。Next, a method for manufacturing the breathable material 2 will be specifically explained using water-repellent sterile paper as an example.
本発明の撥水性滅菌紙は、バルブ、サイズ剤、紙力、増
強剤、填料、定着剤等公知の製紙用原料を適宜組み合わ
せて抄紙し、抄紙時あるいは抄紙俊含浸、塗上を施すこ
とにより製造することができる。The water-repellent sterilized paper of the present invention is made by appropriately combining known papermaking raw materials such as valves, sizing agents, paper strength, reinforcing agents, fillers, fixing agents, etc., and by applying papermaking impregnation and coating at the time of papermaking or by applying papermaking impregnation and coating. can be manufactured.
この場合、必要な透気度は、使用するバルブのＮ材／Ｌ
材比、叩解度、填料、デンプン等製紙資材の内添量、抄
紙時のプレス圧、秤量、樹脂等の含浸量、塗布量を適宜
選択することにより得られる。In this case, the required air permeability is N material/L of the valve used.
It can be obtained by appropriately selecting the material ratio, beating degree, filler, internal addition amount of papermaking materials such as starch, press pressure during paper making, basis weight, amount of impregnated resin, etc., and coating amount.
又、必要なｐＨ値は適当な塩基性物質を紙料中又は、含
浸剤や塗工液中に添加し、耐水性を付与するためには、
紙料中に、サイズ剤、撥水剤、湿潤紙力増強剤、合成樹
脂エマルジョン等の耐水性付与剤を添加するか、あるい
は、これらを含浸、塗布する。In addition, the necessary pH value can be determined by adding an appropriate basic substance to the paper stock, impregnating agent, or coating liquid to impart water resistance.
A water resistance imparting agent such as a sizing agent, a water repellent, a wet paper strength enhancer, a synthetic resin emulsion, etc. is added to the paper stock, or these are impregnated or coated.
ｐＨａ整に使用する塩基性物質としては、例えばリン酸
ナトリウム、リン酸カリウム、炭酸カルシウム、ホウ砂
等のアルカリ金属塩類、酸化カルシウム、酸化マグネシ
ウム等のアルカリ金属の酸化物、水酸化カルシウム、水
酸化マグネシウム等のアルカリ金属の水酸化物、酢酸ア
ンモニウム、はう酸アンモニウム等のアンモニウム塩、
エタノールアミン、トリエタノールアミン等のアミン類
あるいは、これらの混合物が適している。Basic substances used for pH adjustment include, for example, alkali metal salts such as sodium phosphate, potassium phosphate, calcium carbonate, and borax, alkali metal oxides such as calcium oxide and magnesium oxide, calcium hydroxide, and hydroxide. Alkali metal hydroxides such as magnesium, ammonium salts such as ammonium acetate and ammonium oxalate,
Amines such as ethanolamine, triethanolamine, or mixtures thereof are suitable.
次に実施例により本発明に係る材料の製造態様を例示す
る。Next, examples will illustrate the manufacturing mode of the material according to the present invention.
〈実施例〉
ＮＢＫＰ３０％、ＬＢＫＰ７０％からなるバルブ１００
部をカナダ標準濾水度（Ｃ８Ｆ）３５０ｄまで叩解し、
次いでこのパルプスラリーに、ポリアミドポリアミンエ
ピクロルヒドリン樹脂を対パルプ１．０部（固形分）、
アルキルケテンダイマー０．７部、（固形分）を添加し
た。次いでこの紙料を長網抄紙□により抄紙し、サイズ
プレスにてポリアクリルアミド樹脂９，０部（濃度１０
％）、スチレンマレイン酸系表面サイズ剤３．４部、（
濃度３０％）、ホウ砂０．６部、パラフィンワックスエ
マルジョン１．０部（１１度３０％）、水８６部から成
る液で、表面サイズを施した。得られた通気性材料は坪
量７０３４、Ｊ Ｉ Ｓ Ｐ８１１７法による透気度が
３５秒、Ｊ Ｉ Ｓ Ｐ８１３３？ＡＬＪニルｐＨ値カ
８、Ｊ Ｉ Ｓ Ｐ８１２’法によるサイズ度が４５秒
でピンホールがなく、糸状菌やバクテリアの培養用通気
性材料として好適なものであった。<Example> Valve 100 consisting of 30% NBKP and 70% LBKP
part was beaten to Canadian standard freeness (C8F) 350d,
Next, to this pulp slurry, 1.0 part (solid content) of polyamide polyamine epichlorohydrin resin was added to the pulp slurry.
0.7 part (solid content) of alkyl ketene dimer was added. Next, this paper stock was made into paper using Fourdrinier paper □, and 9.0 parts of polyacrylamide resin (concentration 10
%), 3.4 parts of styrene-maleic acid surface sizing agent, (
The surface size was applied with a liquid consisting of 0.6 parts of borax, 1.0 part of paraffin wax emulsion (11 degrees 30%), and 86 parts of water. The obtained breathable material has a basis weight of 7034, an air permeability of 35 seconds according to the JIS P8117 method, and a JIS P8133? It had an ALJNil pH value of 8, a sizing degree of 45 seconds according to the JIS P812' method, and no pinholes, making it suitable as an air-permeable material for culturing filamentous fungi and bacteria.
本発明に係る材料２は、図中Ａで示す通り、従来のプラ
スチック懺ビンや、袋等の培養容器１の通気部分として
、容器本体の一部を取り除き、その部分にヒートシール
あるいは接着剤等により貼着し糸状菌やバクテリアの培
養に使用される。As shown by A in the figure, the material 2 according to the present invention is used as a ventilation part of a culture container 1 such as a conventional plastic bottle or bag by removing a part of the container body and applying heat sealing or adhesive to that part. It is used for culturing filamentous fungi and bacteria.
ちなみに、培養する糸状菌の分類を表１に示す。Incidentally, Table 1 shows the classification of filamentous fungi to be cultured.
表 １
糸状菌の分類学的分類
く効果〉
ところで、本発明に係る材料の最も特徴のある使用方法
は、図中８で示されるように、培養容器１の培地３接触
部分に使用することができることである。これは従来の
通気性材料には到底考えられないことであるが、前述し
たように、本発明に係る材料には、糸状菌が付着しても
糸状菌が成長して、その目を塞ぐことがないという他の
通気性材料にはない特性を持っているためである。これ
により、従来方法では培地３の下部等の通気が不充分な
ため培地３全体を有効に利用できなかったが、本発明に
係る通気性材料２を容器１の培地３接触部分に使用する
ことにより培地全体の通気性が良くなり、培地３全体を
糸状菌の成長に使用できるため、目的とする糸状類の収
量を飛躍的に増やすことができる。Table 1 Effect of taxonomic classification of filamentous fungi> By the way, the most characteristic method of using the material according to the present invention is that it can be used in the part of the culture container 1 that comes in contact with the medium 3, as shown by 8 in the figure. It is possible. This is completely unthinkable for conventional breathable materials, but as mentioned above, even if filamentous fungi adhere to the material of the present invention, the filamentous fungi will grow and block the eyes. This is because it has a characteristic that other breathable materials do not have. As a result, in the conventional method, the entire culture medium 3 could not be used effectively due to insufficient ventilation in the lower part of the culture medium 3, but the breathable material 2 according to the present invention can be used in the part of the container 1 that comes into contact with the culture medium 3. This improves the aeration of the entire medium, allowing the entire medium 3 to be used for the growth of filamentous fungi, thereby dramatically increasing the yield of the desired filamentous fungi.
図は本発明に係る通気性材料の使用状況を示す概要図で
ある。The figure is a schematic diagram showing how the breathable material according to the present invention is used.
Claims (3)
つ透気度が空気透過度試験機で５〜５０秒を有する膜体
から成ることを特徴とする菌類の培養用通気性材料。(1) Air permeability for culturing fungi, characterized by comprising a membrane having a pH of 7 to 11, a weight of 30 g/m^2 or more, and an air permeability of 5 to 50 seconds using an air permeability tester. material.
に記載の菌類の培養用通気性材料。(2) The breathable material for culturing fungi according to claim 1, wherein the membrane is water-repellent sterile paper.
囲第１項に記載の菌類の培養用通気性材料。(3) The breathable material for culturing fungi according to claim 1, wherein the membrane is polyolefin fiber paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29710886A JPH0644858B2 (en) | 1986-12-12 | 1986-12-12 | Breathable material for fungal culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29710886A JPH0644858B2 (en) | 1986-12-12 | 1986-12-12 | Breathable material for fungal culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63148977A true JPS63148977A (en) | 1988-06-21 |
| JPH0644858B2 JPH0644858B2 (en) | 1994-06-15 |
Family
ID=17842314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29710886A Expired - Fee Related JPH0644858B2 (en) | 1986-12-12 | 1986-12-12 | Breathable material for fungal culture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0644858B2 (en) |
-
1986
- 1986-12-12 JP JP29710886A patent/JPH0644858B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0644858B2 (en) | 1994-06-15 |
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