JPS6248374A - Tool for clinical test - Google Patents

Abstract

PURPOSE:To prevent the clouding of a transparent part and make possible sure observation and accurate judgment of the interior of a tool for clinical tests, by coating the inner surface of the transparent part of the tool for clinical tests having a transparent top for observing the interior with a hydrophilic material. CONSTITUTION:In a tool for clinical tests equipped with a top having a transparent part capable of observation of the interior, a region of the inner surface of the transparent part is coated with a hydrophilic material. A water-soluble high polymer such as water-soluble silicone, polyvinylpyrrolidone, methyl cellulose or gelatin, or surfactant, e.g. soap, may be used as the hydrophilic material A separation and cultivation tool 10 of bacteria in blood, laboratory dish 30 and porous container 40 are cited as the tool for clinical tests and the region of the inner surfaces of the respective lid bodies 12, 32 and 42 are coated with the hydrophilic material.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to instruments for clinical testing, such as a blood bacteria isolation incubator and a Petri dish-1 multi-hole container.

[Prior Art] Conventionally, for example, a blood bacteria isolation incubator described in Japanese Patent Application Laid-open No. 59-1!18085, a multi-hole container described in Japanese Utility Model Application Publication No. 80-67100, and bacterial testing, culture, etc. Petri dishes and the like used for this purpose have at least the cup part of the container body as a transparent part, and the inside of each can be observed. As a result, for example, the culture state of blood bacteria in a blood bacteria isolation incubator, the culture state or color reaction state of the culture solution filled in each chamber in a multi-hole container, and the internal state of a petri dish. The culture state of bacteria on the agar medium filled in the container can be easily observed through each transparent part.

[Problems to be Solved by the Invention] However, in each of the above-mentioned conventional clinical test instruments, although the inside can be observed through the transparent part, for example, water vapor generated from culture solution, reagents, etc. Therefore, clouding is likely to occur on the inner surface of the transparent part. In particular, when each of the clinical test instruments described above was placed in an incubator and heated, a large amount of clouding occurred on the inner surface of the transparent part, making it difficult to observe the inside. Furthermore, in order to get a closer look, I sometimes opened the lid to observe it. As a result, bacteria in the air got mixed in, and observation the next day revealed colonies formed by these bacteria.
The target colonies were mixed together, resulting in incorrect judgment.

[Object of the Invention] An object of the present invention is to provide a clinical test instrument that prevents clouding of the transparent portion, allows reliable observation of the inside of the clinical test instrument, and allows correct judgment.

[Means for Solving the Problems] In order to achieve the above-mentioned object, the present invention provides a clinical testing instrument comprising an upper surface having a transparent part that allows observation of the inside, in which a hydrophilic material is provided on the inner surface of the transparent part. It is decided to cover the

Further, in the present invention, the testing instrument is a blood bacteria isolation and culturing device.

Further, in the present invention, the testing instrument is a petri dish.

Further, in the present invention, the testing instrument is a multi-hole container.

Further, in the clinical test instrument according to the present invention, the hydrophilic material is a water-soluble polymer substance.

Further, in the clinical test instrument according to the present invention, the hydrophilic material is used as a surfactant.

[Example] An embodiment of the present invention will be described below with reference to the drawings.

FIG. 1 is a perspective view showing a blood bacteria isolation incubator according to the first embodiment of the present invention, FIG. 2 is a sectional view taken along line II-II in FIG. 1, and FIG. FIG.

The blood bacteria isolation incubator 10 includes a container main body 11 having a substantially cylindrical shape.
and a lid 12 that covers the top opening of the container body 11 so as to be openable and closable. The container body 11 and the lid 12 are each made of a transparent resin material so that the inside can be observed. Concentric protrusions 13 are provided on the inner bottom of the container body 11.
A vent hole 14 is formed at the center of the inner bottom of the container body 11. A water absorbent body 15 and a filter 16 are disposed above the protrusion 13 at the inner bottom of the container body 11. The water absorbent body 15 and the filter 16 are each bonded and integrated with the filter 16 facing upward, and the peripheral portions of the water absorbent body 15 and the filter 16 are joined to the side wall of the container body 11 without any gaps. Water absorbing body 15 and filter 16
For adhesion, heat sealing using low melting point polymeric fibers is used so as not to impede the filtration performance of the filter 16. Further, the adhesive body of the water absorbent body 15 and the filter 16 is pressed into the bottom of the container body 11 by a ring body.

The filter 16 is for filtering bacteria in the blood, and has numerous holes large enough to prevent bacteria from passing through. Filter 1
The pore size of No. 6 is 0.75 microns or less, preferably 0.
．． It is about 45 microns. The material of the filter 16 is not particularly limited as long as it is inert to blood, but typical examples include nitrocellulose, polycarbonate, polyamide,
Examples include cellulose ester, and a commercially available product is Millibore (a product of Millibore Corporation).
, Metricel (manufactured by Gelman Instrument Company), etc. The filter 16 is desirably treated with a parent wood treatment by a method known per se so that blood is easily absorbed.

The water absorbent body 15 absorbs and retains the filtered blood and culture medium filtered by the filter 16, and provides nutrients to the bacteria captured on the filter 16. It is desirable that the water absorbent body 15 has the ability to absorb almost all of the filtrate, and suitable materials include cellulose filter paper, nonwoven fabric, etc.

Further, the space 17 formed between each of the protrusions 13 below the water absorbent body 15 allows blood that cannot be absorbed by the water absorbent body 15 to be stored therein.

On the other hand, the lid body 12 is provided with a blood dispensing section 18 made of rubber. The blood dispensing section 18 is superimposed on a hole formed in the lid body 12, and blood is dispensed through the dispensing section 18 by penetrating the injection needle 20 of the syringe 19 into the dispensing section 18. It is done in the state. As a result, it becomes possible to inject the blood collected with the syringe 19 into the container body 11 sealed by the lid 12, and furthermore, it becomes possible to filter the injected blood with the filter 16.

The entire inner surface area of the lid 12 made of a transparent resin material such as polystyrene, polyethylene, polypropylene, etc. is coated with a hydrophilic material 21 as shown in FIG. Hydrophilic material 21
Examples include water-soluble polymer substances and surfactants.

Water-soluble polymer substances include water-soluble silicone, polyethylene glycol, polyvinylpyrrolidone, methylcellulose, sodium carboxymethylcellulose, starch, gelatin, and the like. Surfactants include soap and the like.

There are various methods of coating the inner surface area of the lid 12 with the hydrophilic material 21, such as coating, spraying, etc. of the hydrophilic material 21. In the example, water-soluble silicone (SH
3771, manufactured by Toshi Silicone Co., Ltd.), and a methanol solution containing 0.1% of the water-soluble silicone was applied to the inner surface area of the lid 12 for coating. The methanol solution was applied using a dropper, and the lid 12 was rotated so that the solution was applied to the entire inner surface area.

Once the methanol solution has sufficiently adhered to the inner surface area, excess solution is removed from the lid 12, and then the lid 12 is allowed to dry. The lid 12 is first dried at room temperature for about 10 minutes, and then in a dryer at 40° C. for about 10 minutes. As a result, the methanol component in the solution is evaporated, and the entire inner surface area of the lid 12 is coated with water-soluble silicone.

Next, the specific operation of the blood bacteria incubator 10 according to the above embodiment will be explained.

First, a predetermined amount of blood is collected from the human body, and this is treated with a hemolytic agent,
Mix well with a solution consisting of anticoagulant and liquid medium. Saponin is preferably used as the hemolytic agent, and sodium amylosulfate, sodium polyanethole sulfate, etc. are used as the anticoagulant. As the liquid medium, those commonly used for the enrichment culture of bacteria can be used without particular limitation. This operation is a pretreatment for the next filtration operation, and is performed for the purpose of destroying red blood cells and preventing blood clots. A predetermined amount of the thus pretreated blood sample is collected with the syringe 19, and the blood is injected into the container body 11 via the dispensing section 18. The injected blood is poured onto the filter 16, and the blood is filtered by the filter 16 due to its own weight. At this time, the bacteria in the blood remain on the filter 16, and the blood that has passed through the filter 16 is absorbed by the water absorbing body 15. Blood that exceeds the amount of water absorbed by the water absorbing body 15 is dripped into the space 17,
It will be accumulated. Further, the air compressed by the injected blood is discharged through the vent hole 14, and the blood is smoothly filtered by its own weight.

In this manner, after filtration is completed, the culture vessel 10 is placed in an incubator and heated.

At this time, the bacteria in the blood are cultured using the moisture in the water absorbent body 15 and the filter 16 as nutrients, and colonies of the bacteria in the blood are generated on the filter 16. The state of colony development is observed by looking through the lid 12 made of a transparent resin material. At this time, since the inner surface area of the 5M body 12 is coated with a hydrophilic material 21 made of water-soluble silicone, even if water vapor is generated inside the container body 11 due to heating, the water vapor will come into contact with the inner surface area and surface. It will be activated. Therefore, the conventional problem of fogging caused by adhesion of tiny water droplets due to water vapor to the inner surface region is eliminated, and the state of colony development can be reliably observed from the outside. Furthermore, since the colony can be observed without opening the lid 12, it is possible to prevent airborne bacteria from getting inside the container body 11, completely eliminating the possibility of interference with subsequent observations and errors in judgment due to contamination. is possible.

In this way, according to the blood bacteria isolation incubator IO according to the present embodiment, the inner surface area of the 1M body 12 is coated with the hydrophilic material 21, so excess adhesion of water vapor to the inner surface area is eliminated, and the lid Clouding of the inner surface of the body 12 is reliably prevented. Thereby, it becomes possible to reliably observe the inside of the container body 11, and for example, it becomes possible to reliably observe the culture state.

FIG. 4 is a perspective view showing a petri dish according to a second embodiment of the present invention, and FIG. 5 is a sectional view taken along the line v--v in FIG. 4.

The petri dish 30 is composed of a container body 31 and a lid 32 that covers the upper opening of the container body 31. Container body 31
and the lid body 32 are made of polystyrene, polyethylene,
It is made of a transparent resin material such as polypropylene, and allows the internal space 33 of the container body 31 to be observed from the outside. The inner surface area 34 of the lid body 32 is coated with a hydrophilic material. As a result, even when a culture medium 35 is formed inside the container body 31 and bacteria are cultured using the culture medium 35 as shown in FIG. 5, for example, the culture state can be observed reliably and easily with the lid 32 covered It becomes possible to do so. That is, even if water vapor is generated in the container sealed by the lid 32 during culture, the hydrophilic material coated prevents the inner surface from becoming cloudy.

The other configurations and operations are the same as in the previous embodiment.

FIG. 6 is a perspective view showing a multi-hole container according to a third embodiment of the present invention, and FIG. 7 is a sectional view taken along the line ■--■ in FIG. 6.

This multi-hole container 40 has a container main body 41 having a generally cubic shape as a whole.
and a lid 42 that covers the upper part of the container body 41. Sixteen small chambers 43 are formed in the container body 41 and arranged in four rows vertically and four rows horizontally, and each small chamber 43 has an opening at the top. The lid 42 covering the container body 41 is configured to cover the upper openings of all the small chambers 43 in a sealed state in the covered state. The container body 41 and the lid 42 are each made of a transparent resin material such as polystyrene, polyethylene, polypropylene, etc., and as a result, the lid 42
It becomes possible to easily observe the inside of each small chamber 43 through the . Furthermore, the inner surface area 44 of the lid body 42 is coated with a hydrophilic material.

As a result, it is no longer necessary to open the lid 42, for example, when each small chamber 43 is filled with a predetermined amount of sample, and the sample in each small chamber 43 is filled with a test solution and its color reaction is observed. be done. Furthermore, it becomes possible to reliably observe the color reaction of the specimen without causing clouding on the inner surface area 43 of the lid body 42. The other configurations and operations are the same as in the previous embodiment.

[Effects of the Invention] As described above, the present invention provides a clinical testing instrument having an L surface with a transparent part that allows observation of the inside, in which the inner surface area of the transparent part is coated with a hydrophilic material. This has the effect of preventing the transparent part from fogging up, allowing the interior of the clinical testing instrument to be observed reliably and without contaminating airborne bacteria.

Furthermore, since the present invention uses a blood bacteria isolation and culturing device as a clinical test instrument, there is an effect that the culture state of blood bacteria can be reliably observed through the transparent part.

Furthermore, in the present invention, since the clinical test instrument is a petri dish, the inside can be reliably observed through the transparent part.

Further, the present invention has the effect that the clinical test instrument is made into a multi-hole container, and the inside of the container can be reliably observed through the transparent part.

Further, since the clinical test device according to the present invention uses a water-soluble polymer substance as the hydrophilic material, it has the effect of reliably preventing fogging of the transparent portion.

Further, since the clinical test device according to the present invention uses a hydrophilic material as a surfactant, it has the effect of reliably preventing the transparent portion from peeling off.

[Brief explanation of the drawing]

FIG. 1 is a perspective view showing a blood bacteria isolation incubator according to the first embodiment of the present invention, FIG. 2 is a sectional view taken along line II-11 in FIG. 1, and FIG. 3 is a m in FIG. FIG. 4 is a perspective view showing a petri dish according to a second embodiment of the present invention, FIG. 5 is a sectional view taken along line v-V in FIG. 4, and FIG. FIG. 7 is a perspective view showing a multi-hole container according to the third embodiment, and FIG. 7 is a cross-sectional view taken along the line ■-■ in FIG. 6.

Claims (6)

[Claims] (1) A clinical test instrument comprising an upper surface having a transparent part that allows the interior to be observed, characterized in that the inner surface of the transparent part is coated with a wood-loving material. (2) The clinical test device according to claim 1, wherein the test device is a blood bacteria isolation and incubator. (3) The clinical testing instrument according to claim 1, wherein the testing instrument is a petri dish. (4) The clinical testing instrument according to claim 1, wherein the testing instrument is a multi-hole container. (5) The clinical test instrument according to claim 1, wherein the hydrophilic material is a water-soluble polymer substance. (6) The clinical test instrument according to claim 1, wherein the wood-philic material is a surfactant.