JPS6240999B2 - - Google Patents

Info

Publication number
JPS6240999B2
JPS6240999B2 JP58038439A JP3843983A JPS6240999B2 JP S6240999 B2 JPS6240999 B2 JP S6240999B2 JP 58038439 A JP58038439 A JP 58038439A JP 3843983 A JP3843983 A JP 3843983A JP S6240999 B2 JPS6240999 B2 JP S6240999B2
Authority
JP
Japan
Prior art keywords
plasmid
prochymosin
dna
gene
ecori
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP58038439A
Other languages
English (en)
Japanese (ja)
Other versions
JPS59166086A (ja
Inventor
Teruhiko Betsupu
Takeshi Uozumi
Katsuhiko Nishimori
Norio Shimizu
Yoshuki Kawaguchi
Shinsei Hidaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP58038439A priority Critical patent/JPS59166086A/ja
Priority to DK136184A priority patent/DK136184A/da
Priority to EP84102451A priority patent/EP0121775A1/en
Priority to NZ207414A priority patent/NZ207414A/en
Priority to AU25357/84A priority patent/AU572827B2/en
Priority to FI840918A priority patent/FI86886C/fi
Priority to BR8401049A priority patent/BR8401049A/pt
Priority to IE559/84A priority patent/IE57264B1/en
Priority to AR84295974A priority patent/AR241795A1/es
Publication of JPS59166086A publication Critical patent/JPS59166086A/ja
Publication of JPS6240999B2 publication Critical patent/JPS6240999B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP58038439A 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 Granted JPS59166086A (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
DK136184A DK136184A (da) 1983-03-09 1984-02-29 Fremgangsmaade til udtrykkelse af et gen, der koder for prochymosin, i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaaden
EP84102451A EP0121775A1 (en) 1983-03-09 1984-03-07 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in E. coli
NZ207414A NZ207414A (en) 1983-03-09 1984-03-07 Expression of prorennin (prochymosin) using recombinant plasmids in an e. coli host
AU25357/84A AU572827B2 (en) 1983-03-09 1984-03-07 Recombinant plasmid containing prochymosin coding gene
FI840918A FI86886C (fi) 1983-03-09 1984-03-07 Nya expressionsplasmider och deras anvaendning i foerfarandet foer expressering av prokymosin koderande gen i e. coli
BR8401049A BR8401049A (pt) 1983-03-09 1984-03-08 Processo para expressar um gene de codificacao de proquimosina em celulas hospedeiras de e.coli.plasmideo de expressao,gene de proquimosina e processo para fazer plasmideos
IE559/84A IE57264B1 (en) 1983-03-09 1984-03-08 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in e.coli
AR84295974A AR241795A1 (es) 1983-03-09 1984-03-09 Metodo para expresar un gen que codifica proquimosina; plasmidos aplicados en ese metodo; metodo para obtenerlos; el gen incorporado a esos plasmidos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58038439A JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Publications (2)

Publication Number Publication Date
JPS59166086A JPS59166086A (ja) 1984-09-19
JPS6240999B2 true JPS6240999B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1987-09-01

Family

ID=12525334

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58038439A Granted JPS59166086A (ja) 1983-03-09 1983-03-09 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Country Status (9)

Country Link
EP (1) EP0121775A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS59166086A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AR (1) AR241795A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU572827B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BR8401049A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DK (1) DK136184A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
FI (1) FI86886C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IE (1) IE57264B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ207414A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (70)

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EP0123928A3 (en) * 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
DE69127829T2 (de) 1990-11-26 1998-03-19 Chiron Corp., Emeryville, Calif. Expression von pace in wirtszellen und verfahren zu dessen verwendung
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
JP4472866B2 (ja) 1997-11-06 2010-06-02 カイロン ソチエタ ア レスポンサビリタ リミタータ ナイセリア抗原
AU1979599A (en) 1998-01-14 1999-08-02 Chiron S.P.A. (neisseria meningitidis) antigens
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
BR9910089A (pt) 1998-05-01 2004-06-08 Chiron Corp Composições e antìgenos de neisseria meningitidis
ES2397918T3 (es) 1999-04-30 2013-03-12 Novartis Vaccines And Diagnostics S.R.L. Antígenos Neisseriales conservados
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
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CN1796404A (zh) 1999-10-29 2006-07-05 启龙有限公司 奈瑟球菌的抗原性肽
JP2003520248A (ja) 2000-01-17 2003-07-02 カイロン エセ．ピー．アー． Ｎ．ｍｅｎｉｎｇｉｔｉｄｉｓ血清型ｂ外膜タンパク質を含む外膜小胞（ｏｍｖ）ワクチン
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US20070059329A1 (en) 2002-11-15 2007-03-15 Nathalie Norais Unexpected surface proteins in meningococcus
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JP4866725B2 (ja) 2003-06-23 2012-02-01 ノース キャロライナ ステイト ユニヴァーシティー フラクトオリゴ糖利用化合物をコードするアシドフィルス菌核酸及びその使用
US7829307B2 (en) 2003-11-21 2010-11-09 Nps Pharmaceuticals, Inc. Production of glucagon-like peptide 2
AU2005211385B2 (en) 2004-02-02 2008-12-11 Ambrx, Inc. Modified human growth hormone polypeptides and their uses
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ES2385114T3 (es) 2007-03-30 2012-07-18 Ambrx, Inc. Polipéptidos de FGF-21 modificados y sus usos
ES2632504T3 (es) 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
US9938333B2 (en) 2008-02-08 2018-04-10 Ambrx, Inc. Modified leptin polypeptides and their uses
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BR112012010531A2 (pt) 2009-10-27 2019-09-24 Novartis Ag "polipeptídeos de modificação meningocócica fhbp"
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IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production

Also Published As

Publication number Publication date
FI840918A0 (fi) 1984-03-07
FI840918L (fi) 1984-09-10
FI86886C (fi) 1992-10-26
EP0121775A1 (en) 1984-10-17
DK136184A (da) 1984-09-10
BR8401049A (pt) 1984-10-16
JPS59166086A (ja) 1984-09-19
FI86886B (fi) 1992-07-15
NZ207414A (en) 1988-02-29
IE840559L (en) 1984-09-09
DK136184D0 (da) 1984-02-29
AU572827B2 (en) 1988-05-19
IE57264B1 (en) 1992-07-01
AU2535784A (en) 1984-09-13
AR241795A1 (es) 1992-12-30

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