JPS62287159A - 抗原・抗体の濃度測定法 - Google Patents
抗原・抗体の濃度測定法Info
- Publication number
- JPS62287159A JPS62287159A JP61130506A JP13050686A JPS62287159A JP S62287159 A JPS62287159 A JP S62287159A JP 61130506 A JP61130506 A JP 61130506A JP 13050686 A JP13050686 A JP 13050686A JP S62287159 A JPS62287159 A JP S62287159A
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- antibody
- antibodies
- antigens
- magnetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000000427 antigen Substances 0.000 claims abstract description 43
- 102000036639 antigens Human genes 0.000 claims abstract description 43
- 108091007433 antigens Proteins 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
- 239000006249 magnetic particle Substances 0.000 claims abstract description 13
- 239000010419 fine particle Substances 0.000 claims description 22
- 239000000523 sample Substances 0.000 claims description 21
- 239000012488 sample solution Substances 0.000 claims description 8
- 238000000691 measurement method Methods 0.000 claims description 4
- 230000004523 agglutinating effect Effects 0.000 abstract 1
- 230000001268 conjugating effect Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 26
- 229910000859 α-Fe Inorganic materials 0.000 description 15
- 239000011859 microparticle Substances 0.000 description 10
- 230000002776 aggregation Effects 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 238000004220 aggregation Methods 0.000 description 7
- 239000004816 latex Substances 0.000 description 7
- 229920000126 latex Polymers 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 229920000053 polysorbate 80 Polymers 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 235000012343 cottonseed oil Nutrition 0.000 description 3
- 239000002385 cottonseed oil Substances 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 239000000854 Human Growth Hormone Substances 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000003270 steroid hormone Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- HMJBXEZHJUYJQY-UHFFFAOYSA-N 4-(aminomethyl)octane-1,8-diamine Chemical compound NCCCCC(CN)CCCN HMJBXEZHJUYJQY-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 229910002535 CuZn Inorganic materials 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 229910003962 NiZn Inorganic materials 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- 108010088160 Staphylococcal Protein A Proteins 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- ZGSPNIOCEDOHGS-UHFFFAOYSA-L disodium [3-[2,3-di(octadeca-9,12-dienoyloxy)propoxy-oxidophosphoryl]oxy-2-hydroxypropyl] 2,3-di(octadeca-9,12-dienoyloxy)propyl phosphate Chemical compound [Na+].[Na+].CCCCCC=CCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COP([O-])(=O)OCC(O)COP([O-])(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COC(=O)CCCCCCCC=CCC=CCCCCC ZGSPNIOCEDOHGS-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000006748 scratching Methods 0.000 description 1
- 230000002393 scratching effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61130506A JPS62287159A (ja) | 1986-06-05 | 1986-06-05 | 抗原・抗体の濃度測定法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61130506A JPS62287159A (ja) | 1986-06-05 | 1986-06-05 | 抗原・抗体の濃度測定法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62287159A true JPS62287159A (ja) | 1987-12-14 |
| JPH0588787B2 JPH0588787B2 (enrdf_load_stackoverflow) | 1993-12-24 |
Family
ID=15035908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61130506A Granted JPS62287159A (ja) | 1986-06-05 | 1986-06-05 | 抗原・抗体の濃度測定法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62287159A (enrdf_load_stackoverflow) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989007270A1 (en) * | 1988-01-29 | 1989-08-10 | Mitsubishi Kasei Corporation | Method for assaying antigen or antibody |
| JPH02281142A (ja) * | 1989-04-24 | 1990-11-16 | Tdk Corp | 抗原または抗体の測定方法 |
| EP0417301A4 (en) * | 1989-02-10 | 1992-01-15 | Shino-Test Corporation | Method for assaying indirect agglutination |
| US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
| US5238811A (en) * | 1988-04-26 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor and superparamagnetic material-labeled body and method for the manufacture of same |
| WO2011155890A1 (en) * | 2010-06-10 | 2011-12-15 | Hemocue Ab | Detection of magnetically labeled biological components |
-
1986
- 1986-06-05 JP JP61130506A patent/JPS62287159A/ja active Granted
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989007270A1 (en) * | 1988-01-29 | 1989-08-10 | Mitsubishi Kasei Corporation | Method for assaying antigen or antibody |
| US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
| US5238811A (en) * | 1988-04-26 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor and superparamagnetic material-labeled body and method for the manufacture of same |
| EP0417301A4 (en) * | 1989-02-10 | 1992-01-15 | Shino-Test Corporation | Method for assaying indirect agglutination |
| JPH02281142A (ja) * | 1989-04-24 | 1990-11-16 | Tdk Corp | 抗原または抗体の測定方法 |
| WO2011155890A1 (en) * | 2010-06-10 | 2011-12-15 | Hemocue Ab | Detection of magnetically labeled biological components |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0588787B2 (enrdf_load_stackoverflow) | 1993-12-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |