JPS6227117Y2 - - Google Patents
Info
- Publication number
- JPS6227117Y2 JPS6227117Y2 JP1984188721U JP18872184U JPS6227117Y2 JP S6227117 Y2 JPS6227117 Y2 JP S6227117Y2 JP 1984188721 U JP1984188721 U JP 1984188721U JP 18872184 U JP18872184 U JP 18872184U JP S6227117 Y2 JPS6227117 Y2 JP S6227117Y2
- Authority
- JP
- Japan
- Prior art keywords
- incubator
- culture
- medium
- connecting tube
- time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000011081 inoculation Methods 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 239000002609 medium Substances 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 241000607142 Salmonella Species 0.000 description 3
- 241000607626 Vibrio cholerae Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 229940118696 vibrio cholerae Drugs 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 210000003298 dental enamel Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
本考案は自動細菌培養接種装置に関し、その目
的とする所は、極めて簡単な操作で容易にしかも
短時間で細菌を培養並びに接種出来る新しい装置
を提供せんとするにある。[Detailed description of the invention] This invention relates to an automatic bacterial culture and inoculation device, and its purpose is to provide a new device that can culture and inoculate bacteria easily and in a short time with extremely simple operations.
従来細菌代表的にはコレラ菌やサルモネラ菌を
検出する場合次の様な方法で行なわれている。即
ち、第１図に示す様に第一培養器６に培養培地７
を投入し、これにコレラ菌やサルモネラ菌の如き
細菌を検出すべき被検体８を導入して所定時間通
常５〜６時間または18〜20時間35℃前後の温度で
培養して第一段の培養を行ない、次いで第一段の
培養物の上澄液の所定量を採取して、別の培養培
地９が予め投入された第二培養器１０に接種し、
これを同温度で所定時間培養した後に、コレラ菌
やサルモネラ菌の如き細菌の有無を調査し検出す
る。この従来の方法に於いては、第一培養器６で
培養した第一培養物の所定量を第二培養器１０の
培地９に接種するに際しては、スポイトやピペツ
トの如き器具を用いていちいち所定量を採用して
接種しており、煩雑な手間と時間とを要する作業
に依つていた。このためその手間と時間は膨大な
ものとなり、現在簡単且つ容易に、しかも短時間
でこれを行なえる手段の開発が渇望されている。 Conventionally, the following methods have been used to detect bacteria such as Vibrio cholerae and Salmonella. That is, as shown in FIG.
The sample 8 for which bacteria such as Vibrio cholerae and Salmonella are to be detected is introduced into the sample and incubated at a temperature of around 35°C for a predetermined period of time, usually 5 to 6 hours or 18 to 20 hours. Then, a predetermined amount of the supernatant of the first-stage culture is collected and inoculated into a second incubator 10 into which another culture medium 9 has been charged in advance,
After culturing this at the same temperature for a predetermined period of time, the presence or absence of bacteria such as Vibrio cholerae and Salmonella is investigated and detected. In this conventional method, when inoculating a predetermined amount of the first culture cultured in the first incubator 6 into the medium 9 of the second incubator 10, a device such as a dropper or pipette is used to inoculate the medium 9 at each location. Vaccination was carried out using a quantitative method, which required complicated and time-consuming work. For this reason, the effort and time involved are enormous, and there is currently a strong desire for the development of means that can do this simply and easily, and in a short period of time.
本考案者は上記要望に応えるために従来から鋭
意研究を続けてきた結果、所期の目的を達成出来
る装置の開発に成功した。即ち本考案は、第一培
養器１と、これよりも容量の大きい第二培養器２
とを連結管３で連結して、これを単に傾斜するこ
とにより、第一培養器１の内容物を第二培養器２
に移動せしめるようになしたことを特徴とする自
動細菌培養接種装置に係るものである。 The inventor of the present invention has been conducting intensive research to meet the above requirements, and as a result has succeeded in developing a device that can achieve the desired purpose. That is, the present invention comprises a first incubator 1 and a second incubator 2 with a larger capacity.
The contents of the first incubator 1 are transferred to the second incubator 2 by simply tilting the connecting tube 3.
This invention relates to an automatic bacterial culture inoculation device characterized in that the device is adapted to be moved.
本考案を第２図を用いて説明すると、第２図イ
に示す如く第一培養器１と第二培養器２とを連結
管３で連結する。この際第二培養器２は第一培養
器１よりも容量が大きいものを使用し、通常は第
一培養器よりもその直径の大きいものを使用す
る。これ等第一並びに第二培養器としては細菌の
培養が行なえるかぎり特にその形状や材質は限定
されず、広い範囲で適宜に選択して使用出来、具
体的には例えばガラス、合成樹脂、ホウロウ等の
材質で円柱状のものを例示出来、代表例としてガ
ラス製円筒状容器即ちガラス製試験管の如き容器
を挙げることが出来る。ガラス製試験管の如き容
器の場合はたとえば直径10〜20mm程度、高さ50〜
200mm程度のものが好ましく使用出来る。第一培
養器１と第二培養器２とを連結して第一培養器の
内容物を第二培養器２に移動さすための連結管３
としても上記移動が可能なものであれば特に制限
はない。この連結管３の第一培養器１への固定場
所は使用する培地と検体との量に応じて決定さ
れ、これ等両者の合計量４よりも高い箇所５にす
れば良い。該連結管３と第二培養器２との連結
は、連結管３がほぼ水平になる様に連結するのが
好ましい。 The present invention will be explained using FIG. 2. As shown in FIG. 2A, a first incubator 1 and a second incubator 2 are connected by a connecting pipe 3. At this time, the second incubator 2 has a larger capacity than the first incubator 1, and usually has a larger diameter than the first incubator. The shape and material of these first and second culture vessels are not particularly limited as long as they can culture bacteria, and can be appropriately selected from a wide range of materials.Specifically, examples include glass, synthetic resin, enamel, etc. A typical example is a cylindrical container made of glass, ie, a container such as a glass test tube. In the case of containers such as glass test tubes, for example, the diameter is about 10 to 20 mm and the height is 50 to 20 mm.
Something about 200mm can be preferably used. Connecting pipe 3 for connecting the first incubator 1 and the second incubator 2 and transferring the contents of the first incubator to the second incubator 2
However, there is no particular restriction as long as the above movement is possible. The location at which the connecting tube 3 is fixed to the first incubator 1 is determined depending on the amount of the medium and sample to be used, and may be set at a location 5 higher than the total amount 4 of both. The connecting tube 3 and the second incubator 2 are preferably connected so that the connecting tube 3 is substantially horizontal.
本考案の培養接種装置を用いて培養・接種する
に際しては、第一培養器１に従来通り、培地並び
に検体を入れ、所定時間培養し、次いでこれを適
宜な角度で傾斜する。この傾斜により第一培養器
の培養物の上澄部分は第２図ロに示す様に連結管
３を通つて第二培養器２に移動する。この際の角
度を適宜に設定することにより、所望量の上澄液
を第二培養器２に移動して接種出来る。第二培養
器２には接種前に所定の培地を導入しておくが、
この第二培養器２は第一培養器１よりも容量が大
きいので（即ち直径が大きいので）培地の上面は
第一培養器のそれよりも低く、このため、連結管
３を通つて移動して来た第一培養物の上澄液は、
第二培養器２の培地の上面にうまく接種されるこ
ととなる。 When culturing and inoculating using the culture and inoculation device of the present invention, a medium and a specimen are placed in the first incubator 1 in the conventional manner, cultured for a predetermined period of time, and then tilted at an appropriate angle. Due to this inclination, the supernatant portion of the culture in the first incubator is transferred to the second incubator 2 through the connecting pipe 3, as shown in FIG. 2B. By appropriately setting the angle at this time, a desired amount of supernatant liquid can be transferred to the second culture vessel 2 and inoculated. A predetermined culture medium is introduced into the second culture vessel 2 before inoculation.
Since this second incubator 2 has a larger capacity (i.e. has a larger diameter) than the first incubator 1, the top surface of the medium is lower than that of the first incubator, so that the medium can be moved through the connecting tube 3. The supernatant of the first culture was
The upper surface of the medium in the second culture vessel 2 will be successfully inoculated.
このように本考案によれば極めて簡単な装置で
極めて簡単な操作でしかも極めて容易・短時間に
培養・接種を行なうことが出来る。また夜間に於
いても簡単に行なうことも出来る利点もある。 As described above, according to the present invention, culturing and inoculation can be carried out using an extremely simple device and an extremely simple operation, and also extremely easily and in a short time. Another advantage is that it can be easily performed even at night.
第１図は従来の、また第２図は本考案の夫々の
培養・接種装置の一例を示す図面である。
１……第一培養器、２……第二培養器、３……
連結管、４……培地と検体の混合物、５……連結
管設置場所、６……従来の第一培養器、７……培
地、８……検体、９……培地、１０……従来の第
二培養器。
FIG. 1 is a diagram showing an example of a conventional culture/inoculation device, and FIG. 2 is a diagram showing an example of a culture/inoculation device according to the present invention. 1...First incubator, 2...Second incubator, 3...
Connecting tube, 4...Mixture of medium and sample, 5... Connecting tube installation location, 6... Conventional first incubator, 7... Medium, 8... Sample, 9... Medium, 10... Conventional Second incubator.
Claims (1)
培養器２とを一本の連結管３で連結して、これを
単に傾斜することにより、第一培養器１の内容物
を第二培養器２に移動せしめるようになしたこと
を特徴とする自動細菌培養接種装置。 By connecting the first incubator 1 and the second incubator 2, which has a larger capacity, with a single connecting tube 3, and simply tilting this, the contents of the first incubator 1 can be transferred to the second incubator 1. An automatic bacterial culture inoculation device characterized in that the device is moved to an incubator 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1984188721U JPS6227117Y2 (en) | 1984-12-12 | 1984-12-12 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1984188721U JPS6227117Y2 (en) | 1984-12-12 | 1984-12-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61104100U JPS61104100U (en) | 1986-07-02 |
| JPS6227117Y2 true JPS6227117Y2 (en) | 1987-07-11 |
Family
ID=30746186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1984188721U Expired JPS6227117Y2 (en) | 1984-12-12 | 1984-12-12 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6227117Y2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6018080Y2 (en) * | 1981-05-30 | 1985-06-01 | 平四郎 仁木 | culture tube |
-
1984
- 1984-12-12 JP JP1984188721U patent/JPS6227117Y2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61104100U (en) | 1986-07-02 |
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