JPS62269690A - Method for immobilizing enzyme - Google Patents

Method for immobilizing enzyme

Info

Publication number
JPS62269690A
JPS62269690A JP11193386A JP11193386A JPS62269690A JP S62269690 A JPS62269690 A JP S62269690A JP 11193386 A JP11193386 A JP 11193386A JP 11193386 A JP11193386 A JP 11193386A JP S62269690 A JPS62269690 A JP S62269690A
Authority
JP
Grant status
Application
Patent type
Prior art keywords
enzyme
carrier
produce
epoxy
amino group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP11193386A
Inventor
Masato Shimizu
Original Assignee
Yokogawa Electric Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Abstract

PURPOSE: To produce an immobilized enzyme in high immobilization ratio, by immobilizing an enzyme through a spacer consisting of a chain compound containing epoxy groups at both end groups to a carrier.
CONSTITUTION: Silica gel is treated with an acid, etc., to produce an amino group-containing carrier to which amino group(-NH2) is introduced. The carrier is blended with a chain compound such as ethylene glycol diglycidyl ether containing epoxy groups at both ends and the blend is stirred in water adjusted to pH 9W10 for about 4hr to give an epoxy carrier by covalent bond through the amino group and the epoxy group. An enzyme (e.g. glucose oxidase) is added to the enzyme, shaked in water adjusted to a pH range stable to the enzyme to produce an immobilized enzyme by covalent bond through the second epoxy group. The immobilized enzyme has improved enzyme manifestation activity.
COPYRIGHT: (C)1987,JPO&Japio
JP11193386A 1986-05-16 1986-05-16 Method for immobilizing enzyme Pending JPS62269690A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11193386A JPS62269690A (en) 1986-05-16 1986-05-16 Method for immobilizing enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11193386A JPS62269690A (en) 1986-05-16 1986-05-16 Method for immobilizing enzyme

Publications (1)

Publication Number Publication Date
JPS62269690A true true JPS62269690A (en) 1987-11-24

Family

ID=14573771

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11193386A Pending JPS62269690A (en) 1986-05-16 1986-05-16 Method for immobilizing enzyme

Country Status (1)

Country Link
JP (1) JPS62269690A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0545547A2 (en) * 1991-11-08 1993-06-09 Nec Corporation Immobilized enzyme film, protein immobilized film and process for forming the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0545547A2 (en) * 1991-11-08 1993-06-09 Nec Corporation Immobilized enzyme film, protein immobilized film and process for forming the same

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