JPS62254053A - Separation column for high performance liquid chromatograph - Google Patents
Separation column for high performance liquid chromatographInfo
- Publication number
- JPS62254053A JPS62254053A JP9678986A JP9678986A JPS62254053A JP S62254053 A JPS62254053 A JP S62254053A JP 9678986 A JP9678986 A JP 9678986A JP 9678986 A JP9678986 A JP 9678986A JP S62254053 A JPS62254053 A JP S62254053A
- Authority
- JP
- Japan
- Prior art keywords
- tube
- end seal
- seal
- conical opening
- chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000926 separation method Methods 0.000 title claims description 10
- 239000007788 liquid Substances 0.000 title claims description 7
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 12
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 7
- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 239000000945 filler Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は高速液体クロマトグラフ用分離カラムに係り、
特にデッドボリウム殻最小限とするのに好適の分離スラ
ムに関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a separation column for high performance liquid chromatography,
In particular, it relates to a separation slam suitable for minimizing dead volume shells.
液体クロマトグラフ用分離カラムの構造としては各種の
方式のものが提案されているが、中空のクロマＩ−管、
充填剤の流出を防ぐためのフィルタおよび送液管接続用
配管コネクタをこれらの順序で着脱自在に組み合わせた
もの、クロマト管とコネクタの間にフィルタを設置した
エンドシールを挿入したものなどがよく用いられている
。また、近年、カラムの交換保守の簡便化などを目的と
して、特開昭５８−１５８５５号公報などに開示されて
いる如きエンドシールを用いる方法も提案されている。Various types of structures have been proposed for separation columns for liquid chromatography, including hollow Chroma I-tubes,
Often used are a filter that prevents the flow of the filler from flowing out and a piping connector for connecting the liquid pipe, which are removably combined in this order, or an end seal with a filter installed between the chromatography tube and the connector. It is being Furthermore, in recent years, a method using an end seal as disclosed in Japanese Patent Application Laid-Open No. 58-15855 has been proposed for the purpose of simplifying column replacement and maintenance.
上記した従来の方法では、エンドシールとクロマト管は
平面で接しており、この平面に垂直にかかる抑圧により
シール部が形成されるのが一般的であり、これらの方式
では、接触面積が広いため、エンドシールが弾性体で構
成される場合には、わずかなごみなどの混入により無視
しえないデッドボリウムが生じる場合があることおよび
必ずしも高圧でのシールが完全にならないことなどの問
題がある。この種の問題は、充填剤の粒径が５μｍ以下
となる最近の液体クロマトグラフではビーク′’７１Ｊ
’７ムが従来に比して大幅に小さくなるため。In the conventional methods described above, the end seal and the chromato tube are in contact with each other on a plane, and the seal is generally formed by compression perpendicular to this plane. When the end seal is made of an elastic material, there are problems such as a non-negligible dead volume may be generated due to the inclusion of a small amount of dirt, and the seal may not always be completely sealed under high pressure. This type of problem occurs in recent liquid chromatographs where the particle size of the filler is 5 μm or less.
'7 is significantly smaller than before.
無視し得ないカラム外への拡散の原因となるなどの問題
を引き起こす。This causes problems such as non-negligible diffusion outside the column.
本発明の目的は、デッドボリウムを最小限とすることが
でき、かつ、高圧でのシールを完全にすることができる
高速液体クロマトグラフ用分離カラムを提供することに
ある。An object of the present invention is to provide a separation column for high performance liquid chromatography that can minimize dead volume and provide perfect sealing at high pressure.
〔問題点を解決するための手段〕
上記目的は、中空のクロマｉ・管の端部にこのクロマト
管の内径より大きい直径の円板状フィルタを介して１−
記りロフト管の外径より大きい開口部の円錐状間［コを
有してその底部の中心に貫通細孔を有する弾性体からな
るエンドシールを設置し、このエンドシールの上記円錐
状間１−１の底部に上記円板状フィルタを収容し、送液
管接続用の配管コネクタによって上記エンドシールを上
記クロマト管方向に押圧することによって上記クロマト
管と上記エンドシールの上記円錐状間ｒ」の内壁との間
にシール部を形成した構成どして達成するようにした。[Means for Solving the Problems] The above object is to provide a 1-
An end seal made of an elastic body having a conical opening larger than the outer diameter of the loft pipe and having a through hole at the center of its bottom is installed, -1, the disc-shaped filter is housed in the bottom of the tube, and the end seal is pressed toward the chromato tube by a piping connector for connecting the liquid pipe, thereby creating a conical space between the chromato tube and the end seal. This is achieved by forming a seal between the inner wall of the
［作用〕
エンドシールは、クロマト管が接する側に円錐状開戸を
有しており、その底部の径をクロマト管の小径より小さ
くし、クロマト管の端部にエンドシールの円錐状開口の
内壁を押しっけ、エンドシールの内壁にくい込ませてシ
ール作用をするようにしたので、クロマト管とエンドシ
ール間の耐圧を増すことができ、また、従来の面接触方
式を基本的には線接触方式としたので、デッドボリウム
を最小限とすることができる。[Function] The end seal has a conical opening on the side that contacts the chromato tube, the diameter of the bottom is smaller than the small diameter of the chromato tube, and the inner wall of the conical opening of the end seal is attached to the end of the chromato tube. The sealing effect is achieved by pushing the inner wall of the end seal into the inner wall of the end seal, increasing the pressure resistance between the chromatography tube and the end seal. Since this method is adopted, the dead volume can be minimized.
以下本発明を第１図〜第３図に示した実施例を用いて詳
細に説明する。The present invention will be explained in detail below using the embodiments shown in FIGS. 1 to 3.
第１図は本発明の高速液体クロマトグラフ用分離カラム
の一実施例を示す基本部分の部分断面斜視図である。第
１図においては、中空のクロマト管１の端部に充填剤４
の流出防止用フィルタ３を収容したエンドシール２を取
り付けた構造としである。エンドシール２は、クロマト
管１に接する側に円錐状開口５を有しており、その底部
中心には、液体の流出口である細孔６があけである。ま
た、円錐状開口５の底部の径は、クロマト管１の外径よ
りも小さく、その底部とクロマト管１との間にフィルタ
３を設置しである。なお、フィルタ３はクロマト管１の
内径より大きい直径のものとしである。FIG. 1 is a partially sectional perspective view of the basic part of an embodiment of a separation column for high performance liquid chromatography according to the present invention. In FIG. 1, a filler 4 is placed at the end of a hollow chromatography tube 1.
It has a structure in which an end seal 2 containing an outflow prevention filter 3 is attached. The end seal 2 has a conical opening 5 on the side in contact with the chromatography tube 1, and a pore 6, which is a liquid outlet, is bored in the center of the bottom. Further, the diameter of the bottom of the conical opening 5 is smaller than the outer diameter of the chromato tube 1, and the filter 3 is installed between the bottom and the chromato tube 1. Note that the filter 3 has a diameter larger than the inner diameter of the chromato tube 1.
第２図は第１図のクロマト管の使用状態の一実施例を示
す部分断面斜視図である。クロマト管１は、その両端を
フィルタ３．エンドシール２を介して配啼コネクタ７に
よって挾み込んであり、このコネクタ７とカートリッジ
チューブ８との間はネジ構造によって押えつけである。FIG. 2 is a partially sectional perspective view showing an embodiment of the chromato tube shown in FIG. 1 in a state of use. The chromato tube 1 has both ends connected to filters 3. A feeding connector 7 is inserted through the end seal 2, and the connector 7 and the cartridge tube 8 are held together by a screw structure.
このとき、クロマト管１の両端部がそれぞれエンドシー
ル２の円錐状開口５の内壁に押し付けられ、エンドシー
ル２が弾性体であれば、その内壁内にくい込んでシール
部を構成する。そして、エンドシール２の円錐状開口５
の開口角度を調節することにより、クロマト管１を実質
的にフィルタ３に接するまで押し付けることができる。At this time, both ends of the chromatography tube 1 are pressed against the inner wall of the conical opening 5 of the end seal 2, and if the end seal 2 is an elastic body, they are sunk into the inner wall to form a seal. Then, the conical opening 5 of the end seal 2
By adjusting the opening angle of the filter 3, the chromatography tube 1 can be pressed until it substantially contacts the filter 3.
また、第２図において、コネクタ７とエンドシール２は
フィルタ３と反対側の平面で接触し、シール部を構成す
る。なお、９は配管、１０はコネクタネジ、１１はブエ
ラルであるゆ
次に、具体的実施例について説明する。クロマト管１と
して内径１ｍｍ、外径２＋ａｍのステンレス製の中空鋼
管を用い、エンドシール２はフッソ樹脂製とし１円錐状
口部の径が２ 、 ５ ａｍで、底部から開口部までの
高さがａｌｌｌｌｌとし、また、エンドシール２の底部
中心には直径０．２ｍｍの補孔６をあけ、フィルタ３と
しては、直径１．５ｍ＋ｎ、Ｊヴさ０．５北の繊維状フ
ィルタ板を使用した。これらを第２図に示すように、カ
ートリッジチューブ８に組み込んで両端をコネクタ７に
よって挾み込んで使用した結果、耐圧４００ｋｇ／ｃＩ
＋＋”以上のシールを構成できることが確認された。ま
た、デッドボリウムを最小限とすることができた。In addition, in FIG. 2, the connector 7 and the end seal 2 are in contact with each other on the plane opposite to the filter 3, forming a seal portion. In addition, 9 is a pipe, 10 is a connector screw, and 11 is a bulbar. A specific example will be described next. A stainless steel hollow steel tube with an inner diameter of 1 mm and an outer diameter of 2+ am was used as the chromatography tube 1, and the end seal 2 was made of fluorocarbon resin, the diameter of the conical opening was 2.5 am, and the height from the bottom to the opening was 2.5 am. In addition, a supplementary hole 6 with a diameter of 0.2 mm was made in the center of the bottom of the end seal 2, and a fibrous filter plate with a diameter of 1.5 m+n and a JV of 0.5 north was used as the filter 3. As shown in Fig. 2, when these are assembled into a cartridge tube 8 and used by sandwiching both ends with connectors 7, the pressure resistance is 400 kg/cI.
It was confirmed that it was possible to construct a seal with a size of ++ or more. Also, it was possible to minimize the dead volume.
第３図は本発明の他の実施例を示す第１図に相当する断
面図である。第３図においては、エンドシール２の円錐
状開口５の内壁にクロマト管１の外径に合わせた切り込
み段１２を設けであるが。FIG. 3 is a sectional view corresponding to FIG. 1 showing another embodiment of the present invention. In FIG. 3, the inner wall of the conical opening 5 of the end seal 2 is provided with a cut step 12 that matches the outer diameter of the chromato tube 1.
作用機構は上記と同様である。The mechanism of action is the same as above.
以上説明したように、本発明によれば、従来。 As explained above, according to the present invention, conventional
クロマト管と配管コネクタの間に生じることが多かった
デッドボリウムを最小限とすることができ、また、高圧
でのシールを完全にすることができるという効果がある
。The dead volume that often occurs between the chromato tube and the piping connector can be minimized, and there is also the effect that a perfect seal can be achieved under high pressure.
第１図は本発明の高速液体クロマトグラフ用分離カラム
の一実施例を示す基本部分の部分断面斜視図、第２図は
第１図のクロマト管の使用状態の一実施例を示す部分断
面斜視図、第３図は本発明の他の実施例を示す第１図に
相当する断面図である。
１・・・クロマト管、２・・・エンドシール、３・・・
フィルタ、４・・・充填剤、５・・・円錐状開口、６・
・・細孔、７・・・配管コネクタ、８・・・カートリッ
ジチューブ。 、 ・・・−・三・）FIG. 1 is a partial cross-sectional perspective view of the basic part showing an embodiment of the separation column for high-performance liquid chromatography of the present invention, and FIG. 2 is a partial cross-sectional perspective view showing an example of the chromatography tube of FIG. 1 in use. 3 are sectional views corresponding to FIG. 1 showing another embodiment of the present invention. 1... Chromato tube, 2... End seal, 3...
Filter, 4... Filler, 5... Conical opening, 6.
... Pore, 7... Piping connector, 8... Cartridge tube. , ・・・−・３・）
Claims (1)
る配管コネクタとからなる高速液体クロマトグラフ用分
離カラムにおいて、前記クロマト管の端部に該クロマト
管の内径より大きい直径の円板状フィルタを介して前記
クロマト管の外径より大きい開口部の円錐状開口を有し
てその底部の中心に貫通細孔を有する弾性体からなるエ
ンドシールを設置し、該エンドシールの前記円錐状開口
の底部に前記円板状フィルタを収容し、送液管接続用の
前記配管コネクタによつて前記エンドシールを前記クロ
マト管方向に押圧することによつて前記クロマト管と前
記エンドシールの前記円錐状開口の内壁との間にシール
部を形成した構成としたことを特徴とする高速液体クロ
マトグラフ用分離カラム。 ２、前記エンドシールはフッソ樹脂よりなる特許請求の
範囲第１項記載の高速液体クロマトグラフ用分離カラム
。[Scope of Claims] 1. A separation column for high performance liquid chromatography consisting of a hollow chromatography tube, a filter, and a piping connector for connecting a liquid feeding tube, wherein the end of the chromatography tube has a diameter larger than the inner diameter of the chromatography tube. An end seal made of an elastic body having a conical opening larger than the outer diameter of the chromatography tube and a through hole in the center of the bottom is installed through the disc-shaped filter, and the end seal is The disk-shaped filter is accommodated in the bottom of the conical opening, and the end seal is pressed toward the chromato tube by the piping connector for connecting the liquid pipe, thereby connecting the chromato tube and the end seal. A separation column for high performance liquid chromatography, characterized in that a sealing portion is formed between the inner wall of the conical opening and the inner wall of the conical opening. 2. The separation column for high performance liquid chromatography according to claim 1, wherein the end seal is made of fluorocarbon resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9678986A JPS62254053A (en) | 1986-04-28 | 1986-04-28 | Separation column for high performance liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9678986A JPS62254053A (en) | 1986-04-28 | 1986-04-28 | Separation column for high performance liquid chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62254053A true JPS62254053A (en) | 1987-11-05 |
Family
ID=14174404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9678986A Pending JPS62254053A (en) | 1986-04-28 | 1986-04-28 | Separation column for high performance liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62254053A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006521561A (en) * | 2003-03-07 | 2006-09-21 | ウオーターズ・インベストメンツ・リミテツド | Capillary liquid transport device |
| JP2021131235A (en) * | 2020-02-18 | 2021-09-09 | 東ソー株式会社 | Liquid chromatography adapter |
-
1986
- 1986-04-28 JP JP9678986A patent/JPS62254053A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006521561A (en) * | 2003-03-07 | 2006-09-21 | ウオーターズ・インベストメンツ・リミテツド | Capillary liquid transport device |
| JP2021131235A (en) * | 2020-02-18 | 2021-09-09 | 東ソー株式会社 | Liquid chromatography adapter |
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