JPS62167460A - Electrophoretic apparatus - Google Patents

Electrophoretic apparatus

Info

Publication number
JPS62167460A
JPS62167460A JP60269783A JP26978385A JPS62167460A JP S62167460 A JPS62167460 A JP S62167460A JP 60269783 A JP60269783 A JP 60269783A JP 26978385 A JP26978385 A JP 26978385A JP S62167460 A JPS62167460 A JP S62167460A
Authority
JP
Japan
Prior art keywords
gel
plate shaped
unit
plate
pair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP60269783A
Other languages
Japanese (ja)
Other versions
JP2569474B2 (en
Inventor
Tsutomu Nishine
勤 西根
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP60269783A priority Critical patent/JP2569474B2/en
Publication of JPS62167460A publication Critical patent/JPS62167460A/en
Application granted granted Critical
Publication of JP2569474B2 publication Critical patent/JP2569474B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

PURPOSE:To automatic migration operation and to attain to enhance the reproducibility of operation and to conserve labor, by simple constitution such that the titled apparatus is constituted of a pair of plate shaped members capable of grasping a flat plate shaped gel from the front and back sides thereof in a detachable manner. CONSTITUTION:The titled apparatus is constituted of a pair of plate shaped members 1, 2 capable of gripping a flat plate shaped gel G from the front and back sides thereof in a detachable manner,. One plate shaped member 2 consists of a pair of electrode units 11, 14 opposed to both sides in a migration direction, the specimen introducing unit 12 adjacent to the opposed side of the electrode unit 11 and the polyfunctional unit 13 positioned between the specimen introducing unit 12 and the electrode unit 14. For example, when the flat plate shaped gel G with a thickness of 0.5mm fixed to the single surface of a polyester resin film with a thickness of 0.2mm is grasped between both plate shaped members 1, 2 so as to direct the fixing surface downwardly, high voltage is applied to the electrode units 11, 14 through a power source 9 and a controller 8 and the electrophoresis of the flat plate shaped gel G is automatically performed without opening one gel surface and the objective component, for example, the objective macromolecular component in the gel is separated into flat plate shaped gel G and arranged.

Description

【発明の詳細な説明】 (イ)産業上の利用分野 この発明は電気泳動装置に関し、更に詳しくは、平板状
ゲルの電気泳動を簡便に行なうことができる電気泳動装
置に関する。
DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to an electrophoresis device, and more particularly to an electrophoresis device that can easily perform electrophoresis of a flat gel.

(ロ)従来の技術及び発明が解決しようとする問題点 従来、片面を泳動用支持体、例えばガラス、ポリエステ
ル樹脂などに固定された平板状ゲルは、水平にして、片
面を開放したまま泳動するか、又はあらためて二枚のガ
ラス板の間に挾持して垂直状態にて泳動していた。
(B) Problems to be solved by the conventional technology and the invention Conventionally, a plate-shaped gel with one side fixed to a support for electrophoresis, such as glass or polyester resin, is placed horizontally and electrophoresed with one side open. Or, it was sandwiched between two glass plates and migrated vertically.

前者の場合、片面が開放状態であるため泳動中のジュー
ル熱などにより泳動用ゲルから水分が蒸発し、泳動用ゲ
ルの組成が変化するなど、開放された泳動用ゲルと大気
との相互作用を慎重に制御する必要があった。後者の場
合は、操作性の面から、せっかく用いられている泳動用
支持体の利点が生かされていないという問題があった。
In the former case, since one side is open, water evaporates from the electrophoresis gel due to Joule heat during electrophoresis, and the composition of the electrophoresis gel changes, causing interactions between the open electrophoresis gel and the atmosphere. It had to be carefully controlled. In the latter case, there is a problem in that the advantages of the electrophoresis support used are not fully utilized in terms of operability.

特に泳動後の染色・固定・洗浄処理は、平板状ゲルを両
ガラス板で挾持した状態では、不可能であって、表側の
ガラス板を取り除く操作が必要であり、しかもこのガラ
ス板の除去に際しては平板状ゲルの表面を痛めるおそれ
があった。
In particular, staining, fixing, and washing processes after electrophoresis are impossible when the flat gel is held between both glass plates, and it is necessary to remove the front glass plate. may damage the surface of the flat gel.

(ハ)問題点を解決するための手段 この発明は、平板状ゲルを表・裏から脱・入可能に挾持
し得る一対の板状部材からなり、その一方の板状部材が
、泳動方向両側に対向する1対の電極ユニットと、その
一方電極ユニットの対向側に隣接する試料導入ユニット
と、この試料導入ユニットと他方電極ユニツ]・との間
に位置する多機能ユニットとからなり、 ざらにこの多機能ユニツj〜が、内部に液体流路を形成
した枠ブロックと、この枠ブロック内に液密に移動可能
にはめ込まれた冷却ブロックと、枠ブロックに付設され
、冷却ブロックが枠ブロックに対して移動するときに、
両板状部材の間に挾持される平板状ゲルと冷却ブロック
と枠ブロックとの間に形成される間隙に、枠ブロックの
液体流路を介して染色・固定・洗浄液を循環供給し得る
各ステップ液供給部とからなる電気泳動装置である。
(C) Means for Solving the Problems This invention consists of a pair of plate-like members that can hold a flat gel so that it can be taken out and put in from the front and back, and one of the plate-like members is arranged on both sides in the electrophoresis direction. It consists of a pair of electrode units facing each other, a sample introduction unit adjacent to the opposite side of one electrode unit, and a multifunctional unit located between this sample introduction unit and the other electrode unit. This multifunctional unit is attached to a frame block that has a liquid flow path formed inside it, a cooling block that is movably fitted in a liquid-tight manner within this frame block, and a cooling block that is attached to the frame block. When moving against
Each step in which staining, fixing, and cleaning liquids can be circulated and supplied through the liquid channel of the frame block to the gap formed between the flat gel sandwiched between both plate-shaped members, the cooling block, and the frame block. This is an electrophoresis device consisting of a liquid supply section.

すなわち、この発明は、平板状ゲルを挟持し得る1対の
板状部材で構成され、しかもその−力板状部材自体に、
1対の電極ユニット、試料導入ユニット及び冷却・染色
・固定・洗浄処理しうる多機能ユニットが組み込まれる
ことによって、簡単な構成にて平板状ゲルの電気泳動及
びその後の処理の自動化を可能にし、操作の再現性の向
上及び省力化に寄与する。特に平板状ゲルを1つの装置
に納めた状態にして一連の操作ができるので、平板状ゲ
ルを痛めることもなく、高精度の分析が可能となる。も
ちろん泳動中は平板状ゲルの一方面を開放することがな
いので、蒸発による組成の変化を防止できる。
That is, the present invention is composed of a pair of plate-like members capable of sandwiching a plate-like gel, and the plate-like members themselves have the following properties:
By incorporating a pair of electrode units, a sample introduction unit, and a multifunctional unit that can perform cooling, staining, fixation, and washing processes, it is possible to automate flat gel electrophoresis and subsequent processing with a simple configuration. Contributes to improved reproducibility of operations and labor savings. In particular, since a series of operations can be performed with the flat gel stored in one device, highly accurate analysis is possible without damaging the flat gel. Of course, since one side of the flat gel is not opened during electrophoresis, changes in composition due to evaporation can be prevented.

(ニ)実施例 以下図に示す実施例にもとづいてこの発明を詳述する。(d) Examples The present invention will be described in detail below based on embodiments shown in the figures.

なお、これによってこの発明が限定されるものでない。Note that this invention is not limited to this.

まず第1〜2図において、電気泳動装置(D)は、1対
の板状部材(1) (2)よりなり、両板状部材の間に
は、平板状ゲルを挟持できる。+5) (6) (7)
はその平板状ゲルを挿入(六方向)する際のガイドであ
り、(31Innは挿入位置を接触により検出するセン
サ、(9)は電源回路9、(8)はその回路のコントロ
ーラである。そして(4)は両板状部材の間隔を調整す
るカム、(10)はセンサ(3) (3’)が検出信号
を出力したときのみ作動するカムの駆動機構、(23)
は板状部材(2とガイドf5) +6)との間に介在さ
れたコイルスプリング、&4)は両板状部材の連結アー
ムである。(21)は平板状ゲルを特に押し出す際にガ
イド(7)を逆へ方向に移動されるためのガイド(刀の
押し出し機構である。なJ3、両板状部材(1) +2
)間に平板状ゲルを挿入する場合、センサ(31+3’
)が検出信号を出力しない間は適宜υ報を発する。
First, in FIGS. 1 and 2, the electrophoresis device (D) consists of a pair of plate-shaped members (1) and (2), and a plate-shaped gel can be sandwiched between both plate-shaped members. +5) (6) (7)
are the guides when inserting the flat gel (in six directions), (31Inn is a sensor that detects the insertion position by contact, (9) is the power supply circuit 9, and (8) is the controller of that circuit. (4) is a cam that adjusts the interval between both plate-shaped members; (10) is a cam drive mechanism that operates only when sensor (3) (3') outputs a detection signal; (23)
is a coil spring interposed between the plate-shaped member (2 and guide f5) +6), and &4) is a connecting arm between both plate-shaped members. (21) is a guide (sword extrusion mechanism) for moving the guide (7) in the opposite direction especially when extruding the flat gel. J3, both plate-shaped members (1) +2
), when inserting a flat gel between the sensor (31+3'
) outputs a υ alarm as appropriate while it does not output a detection signal.

而して情報の板状部材(2は、特に第3図において、泳
動方向両側に対向する1対の電極ユニット(11) (
141と、その一方(左方)電極ユニット(11)の対
向側に隣接する試料導入ユニット021と、この試料導
入ユニット面と他方電極ユニットf’、4)との間に位
置する多機能ユニット(13)とからなる。なお、囚は
濾紙である。
The information plate-like member (2, especially in FIG.
141, a sample introduction unit 021 adjacent to the opposite side of one (left) electrode unit (11), and a multifunctional unit ( 13). Note that the material used is filter paper.

そしてこの多機能ユニット03)は、特に第4図〈ωに
おいて、枠ブロック(271と、この枠ブロック内に液
密に移動可能にはめ込まれた冷却ブロック(15)と、
第4図(b+に示す、枠ブロック弼に付設された各ステ
ップ液供給部(8)とからなる。なお、Q81(191
は枠ブロック弼に形成された液体流路、(16)はポン
プ、口は染色・固定・洗浄液タンク、■は冷却ブロック
05)の上下移動手段、(22)はシリコンゴムシート
である。
This multi-functional unit 03), especially in FIG.
Consists of each step liquid supply section (8) attached to the frame block 2 shown in Fig. 4 (b+).In addition, Q81 (191
(16) is a pump, the opening is a dyeing/fixing/washing liquid tank, (2) is a means for vertically moving the cooling block 05), and (22) is a silicone rubber sheet.

かくして、両板状部材+1) (2j間に平板状ゲル(
例えば0.2mmのポリエステル樹脂フィルムに片面固
定された0、5mmのゲル)(G)が固定面を下にして
所定位置に挾持されると、電極ユニット(1υ(14)
に、電源(9)およびそのコントローラ(8)を介して
高電圧が印加され、平板状ゲル(G)の電気泳動が一方
ゲル面を開放することなく(蒸発のおそれなく)自動的
に行なわれる。そしてゲル中の目的成分、例えば目的高
分子成分が平板状ゲル(G)中に分離して配列される。
Thus, the plate-shaped gel (
For example, when a 0.5 mm gel (G) fixed on one side to a 0.2 mm polyester resin film is held in a predetermined position with the fixed surface facing down, the electrode unit (1υ (14)
A high voltage is applied via the power supply (9) and its controller (8), and the electrophoresis of the plate-shaped gel (G) is carried out automatically on the one hand without opening the gel surface (without fear of evaporation). . Then, target components in the gel, for example, target polymer components, are separated and arranged in the flat gel (G).

なお、電気泳動中は冷却ブロック05)により、例えば
SICをサーモモジュールで冷却する電子式冷却がコン
トローラ(8)の制御のもとで行なわれ、平板状ゲル(
G)を所定冷温度に保持している。
During electrophoresis, the cooling block 05) performs electronic cooling, such as cooling the SIC with a thermo module, under the control of the controller (8), and the flat gel (
G) is maintained at a predetermined cold temperature.

以上のごとく、平板状ゲル(G)の電気泳動が終了する
と、第4図(a> 〈b+のごとく、上下移動手段囚が
作動して冷却ブロック05)が上昇しく例えば約10m
m)、その冷却ブロック05)と枠ブロック&7)と平
板状ゲル(G)との間に間隙(C)を形成する。
As described above, when the electrophoresis of the flat gel (G) is completed, as shown in FIG.
m), a gap (C) is formed between the cooling block 05), the frame block &7), and the flat gel (G).

そしてこの間隙に冷却ブロックG5)の液体流路(18
) 09+を介して順次染色・固定・洗浄液がコントロ
ーラ(8)の制御のもと、ポンプ(16)により循環供
給され、平板状ゲル(G)上に目的高分子成分が染色固
定される。
The liquid flow path (18) of the cooling block G5) is inserted into this gap.
) Staining, fixing, and washing solutions are sequentially supplied through the pump (16) under the control of the controller (8), and the target polymer component is dyed and fixed on the flat gel (G).

なお、間隙(C)に各ステップ液を供給するに際しては
、シリコンゴムシート■が平板状ゲル(G)に密着し、
シールをしているが、そのシリコンゴムシート(支)は
着脱可能で洗浄を容易にしている。
In addition, when supplying each step liquid to the gap (C), the silicone rubber sheet ■ is in close contact with the flat gel (G),
Although it is sealed, the silicone rubber sheet (support) is removable and easy to clean.

以上のごとく、目的高分子成分が染色固定された平板状
ゲル(G)はカム(4)の作動による板状部材(2)の
上界と押し出し機構21)によるガイド(7)の移動と
により、電気泳動装置(D>から押し出されてくる。
As described above, the plate-shaped gel (G) in which the target polymer component is dyed and fixed is formed by the upper boundary of the plate-shaped member (2) by the operation of the cam (4) and the movement of the guide (7) by the extrusion mechanism 21). , are pushed out from the electrophoresis device (D>).

第1図に示すこの電気泳動装置(D>は、実際には、第
5図(a)市)に示すごとく、ディスプレイ□□□、平
板状ゲル(G)の挿入・排出日田、操作卓出を備えたハ
ウジング(31)内に内蔵されるのが好ましい。
This electrophoresis apparatus (D> shown in Fig. 1 is actually shown in Fig. 5 (a)) shows a display □□□, insertion and ejection of the flat gel (G), and an operation console. Preferably, it is housed in a housing (31) with a.

(ホ)発明の効果 この発明によれば、簡単な構成にて、泳動操作が自動化
でき、いかも操作の再現性向上及び省力化が可能となる
(e) Effects of the Invention According to the present invention, the electrophoresis operation can be automated with a simple configuration, and it is possible to improve the reproducibility of the operation and save labor.

【図面の簡単な説明】[Brief explanation of drawings]

第1図はこの発明の一実施例を示ずは能説明図、第2図
はその下側板状部材の平面説明図、第3図は上側根状部
材の断面説明図、第4図(ill) (b)は多機能ユ
ニットの異なる状態を示す構成説明図、第5図(a)は
上記実施例を内蔵するハウジングの正面図、第5図化)
は同じく平面図である。 (D)・・・・・・電気泳動装置、[11[2+・・・
・・・板状部材、+11) (14)・・・・・・電極
ユニット、面・・・・・・試料導入ユニット、(15)
・・・・・・冷fJ+ブロック、+17)・・・・・・
染色・固定・洗浄液タンク、08)呪・・・・・・液体
流路。
FIG. 1 is an explanatory diagram showing one embodiment of the present invention, FIG. 2 is a plan explanatory diagram of the lower plate-like member, FIG. 3 is a cross-sectional explanatory diagram of the upper root-like member, and FIG. ) (b) is a configuration explanatory diagram showing different states of the multi-functional unit; FIG. 5(a) is a front view of the housing incorporating the above embodiment; FIG. 5)
is also a plan view. (D)... Electrophoresis device, [11[2+...
... Plate member, +11) (14) ... Electrode unit, surface ... Sample introduction unit, (15)
...Cold fJ + block, +17) ...
Staining/fixing/washing liquid tank, 08) Curse...Liquid flow path.

Claims (1)

【特許請求の範囲】 1、平板状ゲルを表・裏から脱・入可能に挾持し得る一
対の板状部材からなり、その一方の板状部材が、泳動方
向両側に対向する1対の電極ユニットと、その一方電極
ユニットの対向側に隣接する試料導入ユニットと、この
試料導入ユニットと他方電極ユニットとの間に位置する
多機能ユニットとからなり、 さらにこの多機能ユニットが、内部に液体流路を形成し
た枠ブロックと、この枠ブロック内に液密に移動可能に
はめ込まれた冷却ブロックと、枠ブロックに付設され、
冷却ブロックが枠ブロックに対して移動するときに、両
板状部材の間に挾持される平板状ゲルと冷却ブロックと
枠ブロックとの間に形成される間隙に、枠ブロックの液
体流路を介して染色・固定・洗浄液を循環供給し得る各
ステップ液供給部とからなる電気泳動装置。
[Scope of Claims] 1. Consisting of a pair of plate-like members capable of holding a flat gel so that it can be taken out and put in from the front and back, one of the plate-like members is a pair of electrodes facing each other on both sides in the electrophoresis direction. unit, a sample introduction unit adjacent to the opposite side of one electrode unit, and a multifunction unit located between this sample introduction unit and the other electrode unit, and furthermore, this multifunction unit has a liquid flow inside it. A frame block forming a channel, a cooling block movably fitted in a liquid-tight manner within the frame block, and a cooling block attached to the frame block,
When the cooling block moves relative to the frame block, the liquid flow path of the frame block passes through the gap formed between the flat gel sandwiched between the two plate members and the cooling block and the frame block. An electrophoresis apparatus consisting of a liquid supply section for each step that can circulately supply staining, fixing, and washing liquids.
JP60269783A 1985-11-29 1985-11-29 Electrophoresis device Expired - Fee Related JP2569474B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP60269783A JP2569474B2 (en) 1985-11-29 1985-11-29 Electrophoresis device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP60269783A JP2569474B2 (en) 1985-11-29 1985-11-29 Electrophoresis device

Publications (2)

Publication Number Publication Date
JPS62167460A true JPS62167460A (en) 1987-07-23
JP2569474B2 JP2569474B2 (en) 1997-01-08

Family

ID=17477093

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60269783A Expired - Fee Related JP2569474B2 (en) 1985-11-29 1985-11-29 Electrophoresis device

Country Status (1)

Country Link
JP (1) JP2569474B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8034223B2 (en) 2001-05-10 2011-10-11 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
US8063969B2 (en) 2003-10-02 2011-11-22 Sony Corporation Imaging device

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150092333A (en) 2013-04-30 2015-08-12 시스템 인스트루먼츠 컴퍼니 리미티드 Electrophoresis method and electrophoresis device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8034223B2 (en) 2001-05-10 2011-10-11 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
US8063969B2 (en) 2003-10-02 2011-11-22 Sony Corporation Imaging device

Also Published As

Publication number Publication date
JP2569474B2 (en) 1997-01-08

Similar Documents

Publication Publication Date Title
US5552321A (en) Temperature controlled culture dish apparatus
US5234559A (en) Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof
US4840714A (en) Electroblotting technique for transferring specimens from a polyacrylamide electrophoresis or like gel onto a membrane
AU607972B2 (en) Automatic electrophoresis apparatus & method
US5209831A (en) Bufferless electrophoresis system and method
US7964077B2 (en) Automated two-dimensional electrophoresis apparatus and instrument constituting the apparatus
US4622124A (en) Device for horizontal electroblotting
GB2450841A (en) Sample separation/adsorption appliance
JPH06180302A (en) Electrophoretic device
US20130105320A1 (en) Slide holder assembly for comet assay
JP6002318B2 (en) Electrophoresis device
JPS62167460A (en) Electrophoretic apparatus
US4059501A (en) Automated electrophoresis unit
JP4668123B2 (en) Gel dyeing and decoloring method, gel electrophoresis decoloring device and gel dyeing and decoloring kit
US4207166A (en) Method and apparatus for electrophoresis
US7077940B2 (en) Strip holder, chamber, cassette, and 2-D gel electrophoresis method and system for performing this method for separating molecules
JPH0718840B2 (en) Two-dimensional electrophoresis method
JPH0329844A (en) Electrophoretic apparatus
JP6353869B2 (en) Biomolecule analyzer
JP4436615B2 (en) Strip holder, chamber and 1-D gel electrophoresis method for separating molecules
CN219391554U (en) Rapid liquid cover film independent temperature control method device
JP3574657B2 (en) Apparatus for fluid treatment of a membrane mounted on a frame
JP5952379B2 (en) Biomolecule analyzer
WO2017162149A1 (en) Device and method of processing sample slide
KR20070060294A (en) Lower protect film remover for lcd

Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees