JPS6147199A - 酵素反応を利用した分析方法 - Google Patents
酵素反応を利用した分析方法Info
- Publication number
- JPS6147199A JPS6147199A JP16914184A JP16914184A JPS6147199A JP S6147199 A JPS6147199 A JP S6147199A JP 16914184 A JP16914184 A JP 16914184A JP 16914184 A JP16914184 A JP 16914184A JP S6147199 A JPS6147199 A JP S6147199A
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- diluted solution
- substrate
- concentration
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004458 analytical method Methods 0.000 title claims description 18
- 238000006911 enzymatic reaction Methods 0.000 title claims description 11
- 239000000758 substrate Substances 0.000 claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 19
- 102000004190 Enzymes Human genes 0.000 abstract description 19
- 229940088598 enzyme Drugs 0.000 abstract description 19
- 239000000243 solution Substances 0.000 abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 8
- 239000001301 oxygen Substances 0.000 abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract description 6
- 239000008103 glucose Substances 0.000 abstract description 6
- 108010015776 Glucose oxidase Proteins 0.000 abstract description 3
- 239000004366 Glucose oxidase Substances 0.000 abstract description 3
- 229940116332 glucose oxidase Drugs 0.000 abstract description 3
- 235000019420 glucose oxidase Nutrition 0.000 abstract description 3
- 229910019142 PO4 Inorganic materials 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 2
- 239000010452 phosphate Substances 0.000 abstract description 2
- 229920006395 saturated elastomer Polymers 0.000 abstract description 2
- 229920002379 silicone rubber Polymers 0.000 abstract description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 2
- 230000035699 permeability Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000006260 foam Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 108010093096 Immobilized Enzymes Proteins 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004186 food analysis Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16914184A JPS6147199A (ja) | 1984-08-13 | 1984-08-13 | 酵素反応を利用した分析方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16914184A JPS6147199A (ja) | 1984-08-13 | 1984-08-13 | 酵素反応を利用した分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6147199A true JPS6147199A (ja) | 1986-03-07 |
| JPH0478278B2 JPH0478278B2 (enrdf_load_stackoverflow) | 1992-12-10 |
Family
ID=15881043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16914184A Granted JPS6147199A (ja) | 1984-08-13 | 1984-08-13 | 酵素反応を利用した分析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6147199A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015518964A (ja) * | 2012-06-06 | 2015-07-06 | アトークアント・ダイアグノスティクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＡｔｔｏｑｕａｎｔ Ｄｉａｇｎｏｓｔｉｃｓ Ｇｍｂｈ | 血液試料中のタンパク質分解カスケードのペプチド分解産物の測定方法 |
-
1984
- 1984-08-13 JP JP16914184A patent/JPS6147199A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015518964A (ja) * | 2012-06-06 | 2015-07-06 | アトークアント・ダイアグノスティクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＡｔｔｏｑｕａｎｔ Ｄｉａｇｎｏｓｔｉｃｓ Ｇｍｂｈ | 血液試料中のタンパク質分解カスケードのペプチド分解産物の測定方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0478278B2 (enrdf_load_stackoverflow) | 1992-12-10 |
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