JPS6118843A - Absorption recording apparatus for centrifugal separator - Google Patents

Abstract

PURPOSE:To enable the recording of a schlieren pattern and absorption distribution, by mounting a knife edge in an UV scanner. CONSTITUTION:A shielding window 10 is attached to the door 15 of a centrifugal separator, and mirrors 12, 13 and slits 11 are attached to a rotor chamber. The light emitted from a lamp 1 forms a focus to a slit 4 through a mask 2 and a lens 3. The light issued from the slit 4 passes through a concave mirror 5, a diffraction lattice 7, a mirror 8, a shielding window 10, the cell of a rotor 14, the shielding window 10 and a mirror 17 to form a focus on a knife edge 24. The light issued from the knife edge 24 is formed into an image on the slit 19 integrated with a photomultiplier 20. The output of the photomultiplier 20 is recorded as density distribution. The knife edge 24 is allowed to have access in order to obtain a schlieren pattern.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a separation centrifuge that can perform separation and analysis by attaching an optical mechanism to the separation centrifuge.

Absorption recording device (UV
The scanner was of a type that expressed the concentration distribution of the sedimenting sample in terms of distance (r) and concentration (C), as shown in Figure 4. When measuring a multi-component sample, errors were likely to occur when reading the midpoint of the interface representing each component. In order to represent and measure the interface as a peak, a separate Schlieren device was required.

There is still no device that combines a UV scanner and Schlieren with an optical system attached to a separation centrifuge.

The purpose of the present invention is to eliminate the drawbacks of the prior art described above.
A knife was installed at the focal point on the exit side of the UV scanner, making it possible to record Schlieren patterns.

The present invention uses an optical system similar to a conventional UV scanner,
A movable knife was provided at the focal point of the concave mirror on the exit side, and the mirror reflected the light and formed an image on the sphere, making it possible to adjust the angle while checking the position of the knife. In addition, we made it possible to change the angle of the mirror so that the image could also be focused on the second slit of the photomaru.

We designed it so that it can also be used as a UV scanner without using a knife.

Embodiments according to the present invention will be described with reference to the drawings. FIG. 1 shows the configuration of the optical system. Separation centrifuge door 15
Two shield windows 10 are installed to keep the rotor chamber in a vacuum.

Mirrors 12 and 13 for reversing light in the rotor chamber,
Install slit 11. On the top surface of the door 15, an optical system on the entrance side and an optical system on the exit side are integrated into one unit and can be attached and detached.

As the lamp 1, an XC lamp or the like that can obtain from ultraviolet to visible light is used. Light emitted from a lamp 1 passes through a mask 2, is collected by a lens 3, and is focused on a slit 4. The light exiting the slit 4 is reflected by a concave mirror 5 and forms an image on a diffraction grating 6. The diffraction grating 6 can be fixed at any position by rotation of the cam 7. The light exiting the diffraction grating 7 returns to the concave mirror 5, passes through the lower side of the slit 4, and is emitted downward by the mirror 8.

The single light that exits the mirror 8 becomes parallel light at the lens 9, passes through the shield window 10, passes through the slit in the rotor chamber,
The direction of the light is changed by the mirror 12, and the light is further emitted upward by the mirror 13, passes through the slit 11, and hits the cell 32 of the rotor. The light passing through the cell 32 passes through another shield window 10 on the door 15 and hits the concave mirror 16 of the top optics. The light that exits the concave mirror 16 is reversed by the mirror 17 and then passes through the knife 2.
A focal point 25 is set at 4, which is further reversed by a movable mirror 18, and an image is formed on a slit 19. Slit 19 is photomaru 20
It is designed to be able to move 21 at a constant speed from the inner reference hole (Ri) 36 to the outer reference hole (RO) 37 of the imaged figure (FIG. 4). slit 14
The light emitted from the photomultiplier 9 is transmitted to the photomultiplier 20 and output as a change in current. Changes in current are amplified by an amplifier, and a recorder records the concentration distribution.

To obtain a Schlieren pattern, it is necessary to move the knife 24 in and out of the focal point 25. In addition, the knob 22 is pressed to change the angle of the movable mirror 18 so that the knife can be visually seen going in and out, and the image is formed on the sufurin 23 so that it can be visually observed from the outside.

The knife 24 is rotatably fixed to a shaft 26 with a pin 30, as shown in FIG. The other end of the knife 24 has a groove so that it slides with the pin 30 of the shaft 26. Screw 3 on shaft 26
0 is the reed, and it moves up and down by rotating the knob 27. The knob 27 is fixed with a handle 29 via a bearing 28.

By rotating the left and right knobs 27, the knife 24 can be set horizontally at the focal point 25 or at an arbitrary angle. Then, a pattern as shown in FIG. 5 can be recorded.

Next, the recorded figures will be explained.

A cell 32 containing a sample 35 and a solvent 34 is inserted into the rotor 14, and a counterweight 33 is inserted into the other.

The cell 32 and counterweight 33 are common to those used in analytical ultracentrifuges.

When the rotor 14 is rotated, the images shown in FIG.
A pattern as shown in FIG. 5 is obtained.

When the mirror 18 is switched and an image is formed on the slit 19 and scanned, the inner reference hole R136, the gas phase 44, the h medium; the scus 40, the sample meniscus 41, and the settling interface 42 are detected.
, outer reference hole 39.

When the knife 24 is used, the interface 42 is represented as a beak 43 as shown in FIG. Rias and R,03
9 is used to find the enlargement rate of the pattern when calculating the distance from the rotation center.

To obtain a Schlieren pattern, the wavelength of a single light is 546
If the recording is set to nm, it is possible to measure at the same density as with the Schlieren optical system. By setting the wavelength to the ultraviolet range (for example, 280 nm or 260 nm), it is also possible to measure dilute solutions of proteins, nucleic acids, and the like.

In this embodiment, as shown in FIG. 1, the spectroscope is provided on the right side and the photomultiply light receiving section is provided on the left side, but the same effect can be obtained even if the left and right sides are interchanged.

Further, although the light emitted from the lamp is passed through a slit using a condensing lens, the same effect can be achieved using a concave mirror. The lens that makes parallel light can also be a concave mirror.

According to the present invention, by incorporating a knife into a UV scanner, it has become possible to record schlieren patterns and record absorption distributions. The shrink optical device and UV scanner device can be integrated to improve functionality.

[Brief explanation of drawings]

FIG. 1 is a perspective view showing the configuration of an optical system according to the present invention, and FIG.
The figure is a cross-sectional view showing the knife section, FIG. 3 is a plan view of the four rotors, and FIGS. 4 and 5 are explanatory diagrams showing patterns and records on the coolant section. 1 is a lamp, 2 is a mask, 3 is a lens, 4 is a slit,
5 is a concave mirror, 6 is a diffraction grating, 7 is a cam, 8 is a mirror, 9
is a lens, 10 is a shield window, 11 is a slit, 12 is a mirror, 13 is a mirror, 14 is a rotor, 15 is a door, 16
is a concave mirror, 17 is a mirror, 18 is a movable mirror, 19 is a slit, 20 is a photomalle, 21 is a moving direction, 22 is a knob, 23 is a sufurin, 24 is a knife, 25 is a focal point, 26 is a shirt), '27 is the knob, 28 is the bearing, 29 is the screw, 30 is the pin, 31 is the screw, 32 is the cell, 33 is the counterweight, 34 is the solvent, 35 is the sample, 36 is the inner reference hole R'% 37 is the outer reference hole RO138 is R
+, a9 is Ro, 40 is the solvent meniscus, 41 is the sample meniscus, 42 is the interface, and 43 is the peak. Name of the name Hitachi Koki Co., Ltd.

Claims (1)

[Claims] In an analytical ultracentrifuge equipped with a removable optical mechanism on the top of the door of the separation ultracentrifuge, a single beam of light is incident from a spectrometer, and the light that has passed through the rotating sample is focused using a concave mirror. This absorption recording device is equipped with a knife that can be moved in and out of that position, and the image is formed on a slit that moves together with the photomultiply to record the concentration distribution of the sample.