JPS6034701B2 - Latex agglutination reaction observation device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a diagnostic device, and more particularly to an advantageous observation device for performing so-called latex agglutination using latex.

In recent years, latex agglutination reactions using antigen-antibody reactions have been
It is widely used as a variety of diagnostic reagents, typical examples of which include pregnancy diagnosis and the qualitative and quantitative determination of FDP (fibrin body degradation products) in dishes and urine. Conventionally, when making these diagnoses, a latex reagent and a test liquid were mixed uniformly on a glass plate with a stick, and then the plate was shaken by hand for 2 to 3 minutes to observe the agglomerated image. However, in the above-mentioned method, individual differences tend to occur in the rocking process and results, and reproducibility is poor. In addition, the swinging operation requires skill, and the manual operation is tiring, which is extremely inconvenient for processing a large number of specimens. The present inventors have arrived at the present invention as a result of various studies, considering that the number of specimens has increased with the spread of diagnostic methods that utilize this latex agglutination reaction, and that conventional manual methods have limitations. According to the present invention, the center portion of a support plate for fixing a glass plate (hereinafter referred to as a sample container) containing a mixture of a latex reagent and a test liquid is supported by a ball joint, and the ball joint is placed at the center. In a latex agglutination reaction observation device consisting of two sets of drive units installed at two points on the circumference, the two sets of drive units are shaken at the same period and at the same time, while maintaining a constant phase difference from each other. A rocking device for a latex aggregation reaction is provided, which is characterized in that it is driven at the same time. The present invention will be explained in detail with reference to the drawings.

In Figure 1, the central part of the flat plate is supported by a fulcrum ○, and there is an arbitrary position A on the circumference of a radius r centered on the fulcrum ○, and a position B on the same circumference, which is 8 angles away from A. is set, and a sine wave vibration of a=Xsinwt is applied to point A in the direction of the arrow. where X is the angular velocity during maximum vibration. Similarly, at point B, a sine wave vibration b=Xsin (t±?) of equal angular velocity and phase advanced (or delayed) by C is applied. For ease of explanation, taking the case of a=900 as an example, when both vibrations a and b are applied to points A and B, respectively, any point on the normal line to the fulcrum 0 on the flat plate The locus of movement of P is shown in FIGS. 2 to 4.

The amplitude x of the motion of point P is on the normal line of fulcrum ○, and is proportional to the distance OP = y' from fulcrum ○, and OP = y' is 0, that is, x20 when extremely close to the flat plate, and the movement of the flat plate is is observed only as a change in the inclination angle from the source plane. Figure 2 shows that when AOB=0=◇, the locus of point P traces a circle. Third
In the case where the figure is 0, 2 m, ..., the locus of point P is a straight line. Me = Bamboo, ... (Trajectory - 1) In the case of a sword, the trajectory is in the opposite direction on the same straight line. Figure 4 shows the locus of point P when the phase difference is small and 0≠0, 2, ..., or m, and it moves on an ellipse with different long and short distance ratios depending on the size of the phase difference. Moving. FIG. 5 is a top view of a rocking device manufactured based on the above principle, and FIG. 6 is a side view of the same. In FIGS. 5 and 6, 1 is a sample container;
2 is a support plate, 3 is a ball joint, 4 is a drive arm A, 5 is a drive arm A
4 is a ball joint for connecting to the support plate 2, 6 is a drive arm A4
7 is a drive arm B, 8 is a cam for causing sinusoidal movement in the
1 is a ball joint for connecting the drive arm B7 to the support plate 2; 9 is a cam for causing the drive arm B7 to perform a sine wave motion; 10 is a rotating shaft for connecting the cam 6 and the cam 9; 11, 12 1 is a bearing for the rotating shaft 10, 13 is a pulley for driving the rotating shaft, 1
4 is a rotation transmission belt, 15 is a pulley directly connected to a motor 16, 16 is a drive motor, and 17 is a board for incorporating the above series of mechanisms. The operation of the apparatus of the present invention will be explained with reference to FIGS. 5 and 6. A sample liquid, which is a mixture of a latex reagent and a test liquid, is placed in a sample container 1 and placed at a predetermined position on a support plate 2. The center portion of the support plate 2, which corresponds to the fulcrum 0 in FIG. 1, is supported by a ball joint 3 so that the support plate 2 can freely move forward, backward, left, and right.
The upper end of the drive arm A4 is connected to the first drive arm via a ball joint 5 so that its movement is not constrained by the inclination of the support plate 2.
It is connected to the support plate 2 at a position corresponding to point A in the figure.
Drive arm B7 also connects support plate 2 via ball joint 8 to B in FIG. 1 for the same reason and method as drive arm A4.
Connected to the level corresponding to the point. The lower end of the drive arm A4 is eccentrically connected to the cam 6, and gives a sine wave motion to the drive arm A4 based on the principle of converting uniform circular motion into linear motion to perform a sine wave motion. The lower end of the drive arm B7 is also connected to the cam 9 for the same reason and method. Cam 6 and Cam 9
The distance from the center of rotation to the lower end connections of drive arm A4 and drive arm B7, respectively, determines the maximum oscillation x of the sinusoidal motion. Cam 6 and drive arm A4 and cam 9 and drive arm B7
If the relative positions of If their relative positions are shifted from each other, the support plate 2 will move in a circular or elliptical manner as shown in FIGS. 2 and 4 depending on the mutual positional relationship. The cams 6 and 9 are connected to each other by a rotating shaft supported by bearings 11 and 12, and are driven at a constant speed by a motor 16 via a pulley 13, a belt 14, and a pulley 15. The sample in the sample container 1 on the support plate 2 swings in response to the movement of the support plate 2, and a shelving operation is performed. According to the device of the present invention, the shaking mixing operation of the latex reagent and test liquid, which was the biggest drawback in conventional latex agglutination reaction observation methods, can be performed mechanically, so there is no room for individual differences. Also, the inspector will not get tired.

In addition, since the rocking time can be easily controlled automatically by using an ordinary timer or the like, it provides an extremely advantageous means for mass processing of pregnancy diagnosis and other tests.

[Brief explanation of drawings]

Fig. 1 is a principle diagram for explaining the operating principle of the device of the present invention, Fig. 2 is a locus of movement of point P when the phase difference between two points A and B on the circumference is = a, and Fig. 3 Figure 4 shows the locus of motion of point P when = 0, J≠0, ? FIG. 3 is a diagram of a time course showing the locus of movement of point P when ≠0. FIG. 5 is a top view of the device of the present invention, and FIG. 6 is a side view.
Figure 2 Hair Figure 3 Hair 4 Figure Hair 5 Figure 6

Claims (1)

[Claims] 1. In an observation device for latex aggregation reaction, which consists of a ball joint provided in the center of the sample container support plate, and two sets of drive units provided at two points on the circumference with the ball joint as the center, two sets of drive units are provided. linear motion by driving the drive parts of each with the same period, the same amplitude, and while maintaining a constant phase difference between them.
A latex aggregation reaction observation device characterized by having a rocking mechanism that allows easy selection of either elliptical motion or circular motion.