JPS5816696A - 酵素活性の測定方法 - Google Patents
酵素活性の測定方法Info
- Publication number
- JPS5816696A JPS5816696A JP56114538A JP11453881A JPS5816696A JP S5816696 A JPS5816696 A JP S5816696A JP 56114538 A JP56114538 A JP 56114538A JP 11453881 A JP11453881 A JP 11453881A JP S5816696 A JPS5816696 A JP S5816696A
- Authority
- JP
- Japan
- Prior art keywords
- ammonia
- concentration
- electrode
- temperature
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002255 enzymatic effect Effects 0.000 title abstract description 4
- 238000005259 measurement Methods 0.000 title description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 109
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 102000004190 Enzymes Human genes 0.000 claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 claims abstract description 20
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 11
- 230000008859 change Effects 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 17
- 150000003863 ammonium salts Chemical class 0.000 claims 1
- 229910021529 ammonia Inorganic materials 0.000 abstract description 47
- 239000000758 substrate Substances 0.000 abstract description 17
- 239000000243 solution Substances 0.000 abstract description 14
- 239000007853 buffer solution Substances 0.000 abstract description 7
- 102000004142 Trypsin Human genes 0.000 abstract description 4
- 108090000631 Trypsin Proteins 0.000 abstract description 4
- 239000003513 alkali Substances 0.000 abstract description 3
- 239000012588 trypsin Substances 0.000 abstract description 3
- 102100032948 Aspartoacylase Human genes 0.000 abstract description 2
- 101000797251 Homo sapiens Aspartoacylase Proteins 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 108010065059 methylaspartate ammonia-lyase Proteins 0.000 abstract description 2
- 108010046334 Urease Proteins 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 239000000872 buffer Substances 0.000 description 15
- 210000002966 serum Anatomy 0.000 description 13
- 230000004044 response Effects 0.000 description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 6
- -1 ammonium ions Chemical class 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- HNXQXTQTPAJEJL-UHFFFAOYSA-N 2-aminopteridin-4-ol Chemical compound C1=CN=C2NC(N)=NC(=O)C2=N1 HNXQXTQTPAJEJL-UHFFFAOYSA-N 0.000 description 2
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 description 2
- 102000006267 AMP Deaminase Human genes 0.000 description 2
- 108700016228 AMP deaminases Proteins 0.000 description 2
- 102000055025 Adenosine deaminases Human genes 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 108010079340 Benzylamine Oxidase Proteins 0.000 description 2
- 102100034798 CCAAT/enhancer-binding protein beta Human genes 0.000 description 2
- 108010080611 Cytosine Deaminase Proteins 0.000 description 2
- 102000000311 Cytosine Deaminase Human genes 0.000 description 2
- 108030007002 Glucosaminate ammonia-lyases Proteins 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- 101710162629 Trypsin inhibitor Proteins 0.000 description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 description 2
- 108030006907 Ureidosuccinases Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WGQKYBSKWIADBV-UHFFFAOYSA-N benzylamine Chemical compound NCC1=CC=CC=C1 WGQKYBSKWIADBV-UHFFFAOYSA-N 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002753 trypsin inhibitor Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- MSTNYGQPCMXVAQ-RYUDHWBXSA-N (6S)-5,6,7,8-tetrahydrofolic acid Chemical compound C([C@H]1CNC=2N=C(NC(=O)C=2N1)N)NC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-RYUDHWBXSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- RSYYQCDERUOEFI-UHFFFAOYSA-N 2-benzamido-5-(diaminomethylideneazaniumyl)pentanoate Chemical compound NC(N)=NCCCC(C(O)=O)NC(=O)C1=CC=CC=C1 RSYYQCDERUOEFI-UHFFFAOYSA-N 0.000 description 1
- VRZJGENLTNRAIG-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]iminonaphthalen-1-one Chemical compound C1=CC(N(C)C)=CC=C1N=C1C2=CC=CC=C2C(=O)C=C1 VRZJGENLTNRAIG-UHFFFAOYSA-N 0.000 description 1
- 101150015935 ATP2 gene Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 108010052875 Adenine deaminase Proteins 0.000 description 1
- 108700023418 Amidases Proteins 0.000 description 1
- 108090000673 Ammonia-Lyases Proteins 0.000 description 1
- 102000004118 Ammonia-Lyases Human genes 0.000 description 1
- 102100023927 Asparagine synthetase [glutamine-hydrolyzing] Human genes 0.000 description 1
- 108010070255 Aspartate-ammonia ligase Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000511343 Chondrostoma nasus Species 0.000 description 1
- 108010059399 Citrullinase Proteins 0.000 description 1
- 102100031565 Cytidine and dCMP deaminase domain-containing protein 1 Human genes 0.000 description 1
- 108010031325 Cytidine deaminase Proteins 0.000 description 1
- 108030006828 D-glutaminases Proteins 0.000 description 1
- 102100037101 Deoxycytidylate deaminase Human genes 0.000 description 1
- 108010073324 Glutaminase Proteins 0.000 description 1
- 102000009127 Glutaminase Human genes 0.000 description 1
- 108010012029 Guanine Deaminase Proteins 0.000 description 1
- 102000013587 Guanine deaminase Human genes 0.000 description 1
- 102000030789 Histidine Ammonia-Lyase Human genes 0.000 description 1
- 108700006308 Histidine ammonia-lyases Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- FORGMRSGVSYZQR-YFKPBYRVSA-N L-leucinamide Chemical compound CC(C)C[C@H](N)C(N)=O FORGMRSGVSYZQR-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 102000002704 Leucyl aminopeptidase Human genes 0.000 description 1
- 108010004098 Leucyl aminopeptidase Proteins 0.000 description 1
- 108010019703 Nicotinamidase Proteins 0.000 description 1
- 108010083499 Ornithine cyclodeaminase Proteins 0.000 description 1
- 108700023158 Phenylalanine ammonia-lyases Proteins 0.000 description 1
- 108030003017 Serine-sulfate ammonia-lyases Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 102000005922 amidase Human genes 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 101150026213 atpB gene Proteins 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 108010015012 dCMP deaminase Proteins 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000003028 enzyme activity measurement method Methods 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 108010025509 omega-amidase Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 108010026352 pterin deaminase Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005460 tetrahydrofolate Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56114538A JPS5816696A (ja) | 1981-07-22 | 1981-07-22 | 酵素活性の測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56114538A JPS5816696A (ja) | 1981-07-22 | 1981-07-22 | 酵素活性の測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5816696A true JPS5816696A (ja) | 1983-01-31 |
| JPH0314440B2 JPH0314440B2 (enrdf_load_stackoverflow) | 1991-02-26 |
Family
ID=14640262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56114538A Granted JPS5816696A (ja) | 1981-07-22 | 1981-07-22 | 酵素活性の測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5816696A (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61144561A (ja) * | 1984-12-17 | 1986-07-02 | Shimadzu Corp | 酵素機能電極 |
| JPS61144560A (ja) * | 1984-12-17 | 1986-07-02 | Shimadzu Corp | 酵素機能電極 |
-
1981
- 1981-07-22 JP JP56114538A patent/JPS5816696A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61144561A (ja) * | 1984-12-17 | 1986-07-02 | Shimadzu Corp | 酵素機能電極 |
| JPS61144560A (ja) * | 1984-12-17 | 1986-07-02 | Shimadzu Corp | 酵素機能電極 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0314440B2 (enrdf_load_stackoverflow) | 1991-02-26 |
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