JPS5763089A - Production of cytosine deaminase and culture medium therefor - Google Patents

Production of cytosine deaminase and culture medium therefor

Info

Publication number
JPS5763089A
JPS5763089A JP13619380A JP13619380A JPS5763089A JP S5763089 A JPS5763089 A JP S5763089A JP 13619380 A JP13619380 A JP 13619380A JP 13619380 A JP13619380 A JP 13619380A JP S5763089 A JPS5763089 A JP S5763089A
Authority
JP
Japan
Prior art keywords
cytosine deaminase
production
culture medium
medium therefor
citric acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP13619380A
Other languages
Japanese (ja)
Inventor
Takuo Sakai
Tooru Katsuragi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP13619380A priority Critical patent/JPS5763089A/en
Publication of JPS5763089A publication Critical patent/JPS5763089A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: A collibacillus capable of producing cytosine deaminase is cultured in a medium which is ajdusted in pH with citric acid to produce cytosine deaminase in high yield.
CONSTITUTION: A collibacillus capable of producing cytosine deaminase such as Eserichia coli K12 is cultured in a medium that contains 0.2W2wt% of citric acid and is adjusted in pH to 6.5W7.5, preferably about 7 at the start of cultivation. When the pH reaches 7.8W8.7, citric acid is added to adjust the pH to 7.0W9.2, preferably 7.8W9.2 and the cultivation is continued in this pH range.
COPYRIGHT: (C)1982,JPO&Japio
JP13619380A 1980-09-30 1980-09-30 Production of cytosine deaminase and culture medium therefor Pending JPS5763089A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13619380A JPS5763089A (en) 1980-09-30 1980-09-30 Production of cytosine deaminase and culture medium therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13619380A JPS5763089A (en) 1980-09-30 1980-09-30 Production of cytosine deaminase and culture medium therefor

Publications (1)

Publication Number Publication Date
JPS5763089A true JPS5763089A (en) 1982-04-16

Family

ID=15169511

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13619380A Pending JPS5763089A (en) 1980-09-30 1980-09-30 Production of cytosine deaminase and culture medium therefor

Country Status (1)

Country Link
JP (1) JPS5763089A (en)

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