JPS57163496A - Kit for cholesterol determination - Google Patents
Kit for cholesterol determinationInfo
- Publication number
- JPS57163496A JPS57163496A JP20678881A JP20678881A JPS57163496A JP S57163496 A JPS57163496 A JP S57163496A JP 20678881 A JP20678881 A JP 20678881A JP 20678881 A JP20678881 A JP 20678881A JP S57163496 A JPS57163496 A JP S57163496A
- Authority
- JP
- Japan
- Prior art keywords
- cholesterol
- hydrogen peroxide
- nocardia
- enzyme preparation
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: An enzyme preparation obtained from a microorganism in Nocardia is used to oxidize cholesterol into Δ4-cholestenone and hydrogen peroxide and the formed hydrogen peroxide is measured to determine the cholesterol in biological fluid.
CONSTITUTION: Nocardia NCIB10554 or NCIB10555 is cultured in a medium containing glycerol as a carbon source and cholesterol, then an enzyme preparation having cholesterol oxidase activity is obtained from the culture cells by surfactant extraction, cell crushing and removal of the residue. The resultant preparation and at least one of reagents for determining the hydrogen peroxide such as a tetravalent titanium compound and xylenol orange are brought into contact with a sample containing cholesterol such as serum to determine the hydrogen peroxide formed by the oxidation of the cholesterol in the sample.
COPYRIGHT: (C)1982,JPO&Japio
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB4409571 | 1971-09-22 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1138478A Division JPS5394994A (en) | 1971-09-22 | 1978-02-03 | Method and kit for determination of cholesterol |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57163496A true JPS57163496A (en) | 1982-10-07 |
JPS6179B2 JPS6179B2 (en) | 1986-01-06 |
Family
ID=10431754
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9518681A Expired JPS6058959B2 (en) | 1971-09-22 | 1981-06-19 | Cholesterol quantitative method |
JP20678881A Granted JPS57163496A (en) | 1971-09-22 | 1981-12-21 | Kit for cholesterol determination |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9518681A Expired JPS6058959B2 (en) | 1971-09-22 | 1981-06-19 | Cholesterol quantitative method |
Country Status (1)
Country | Link |
---|---|
JP (2) | JPS6058959B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07158740A (en) * | 1993-12-03 | 1995-06-20 | Ket & Ket:Kk | Metal gasket |
-
1981
- 1981-06-19 JP JP9518681A patent/JPS6058959B2/en not_active Expired
- 1981-12-21 JP JP20678881A patent/JPS57163496A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS6179B2 (en) | 1986-01-06 |
JPS5747496A (en) | 1982-03-18 |
JPS6058959B2 (en) | 1985-12-23 |
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