JPS5664783A - Apparatus for analyzing enzyme activity - Google Patents

Apparatus for analyzing enzyme activity

Info

Publication number
JPS5664783A
JPS5664783A JP14060979A JP14060979A JPS5664783A JP S5664783 A JPS5664783 A JP S5664783A JP 14060979 A JP14060979 A JP 14060979A JP 14060979 A JP14060979 A JP 14060979A JP S5664783 A JPS5664783 A JP S5664783A
Authority
JP
Japan
Prior art keywords
acid
immobilized enzyme
detectors
hydrogen peroxide
living body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP14060979A
Other languages
Japanese (ja)
Inventor
Fusao Shirato
Yasuhisa Shibata
Hiroyuki Miyagi
Yoshitada Takada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP14060979A priority Critical patent/JPS5664783A/en
Publication of JPS5664783A publication Critical patent/JPS5664783A/en
Pending legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE: To measure the enzyme activity in a living body sample with high accuracy and rapidly, by utilizing the characteristics of an immobilized enzyme, oxygen detectors or hydrogen peroxide detectors.
CONSTITUTION: For measuring the activity of GOT (glutamic acid oxalacetate transaminase), a living body sample injected from a sample inlet 2 of a flow path directed by a pump 11 is passed through the first immobilized enzyme reactor 3 of a pyruvate oxidase with a buffer solution 1 to decompose the pyruvic acid in the sample, and aspartic acid and α-ketoglutaric acid fed from a substrate storage tank 4 are changed into an oxalacetic acid by the GOT in the living body sample. The oxaloacetic acid is then converted into pyruvic acid by an immobilized enzyme reactor 5 of an oxalacetate dehydrocarboxylase. The concentration oxygen or hydrogen peroxide is detected by the first detector 6, and the pyruvic acid in the solution is decomposed by the second immobilized enzyme reactor 7 of a pyruvate oxidase to detect the concentration of oxygen or hydrogen peroxide in the solution by the second detector 8. The output difference between the first and the second detectors 6 and 8 is amplified by an amplifier 9 and recorded in a recorder 10.
COPYRIGHT: (C)1981,JPO&Japio
JP14060979A 1979-10-30 1979-10-30 Apparatus for analyzing enzyme activity Pending JPS5664783A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14060979A JPS5664783A (en) 1979-10-30 1979-10-30 Apparatus for analyzing enzyme activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14060979A JPS5664783A (en) 1979-10-30 1979-10-30 Apparatus for analyzing enzyme activity

Publications (1)

Publication Number Publication Date
JPS5664783A true JPS5664783A (en) 1981-06-02

Family

ID=15272680

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14060979A Pending JPS5664783A (en) 1979-10-30 1979-10-30 Apparatus for analyzing enzyme activity

Country Status (1)

Country Link
JP (1) JPS5664783A (en)

Similar Documents

Publication Publication Date Title
Rigo et al. Polarographic determination of superoxide dismutase
Nanjo et al. Enzyme electrode sensing oxygen for uric acid in serum and urine
Lippi et al. A new colorimetric ultramicromethod for serum glutamicoxalacetic and glutamic-pyruvic transaminase determination
Watanabe et al. Determination of phosphate ions with an enzyme sensor system
ES8507689A1 (en) Detection of the presence of biological activity utilizing infrared analysis.
Preuschoff et al. Chemiluminometric L-lysine determination with immobilized lysine oxidase by flow-injection analysis
Watanabe et al. Simultaneous determination of hypoxanthine and inosine with an enzyme sensor
Preuschoff et al. Photodiode-based chemiluminometric biosensors for hydrogen peroxide and L-lysine
Lavagnini et al. Amperometric lysine bioprobes analysis in feeds
Cheng et al. Amperometric measurement of enzyme reactions with an oxygen electrode using air oxidation of reduced nicotinamide adenine dinucleotide
JPS5664783A (en) Apparatus for analyzing enzyme activity
JPS57110198A (en) Determination of choline esterase activity
Palleschi et al. Flow monitoring of glutamate and aspartate in foods and pharmaceutical products with immobilized bienzyme electrochemical cells
Macholan Recent progress in developing enzyme and tissue‐based membrane electrodes
IE800647L (en) Device for measuring a gaseous anhydride
JPS5516203A (en) Measuring method of activity of microbe
JPS5664782A (en) Apparatus for analyzing enzyme activity
JPS5758895A (en) Analytical method of creatinine and apparatus
JPS5774095A (en) Rapid counting of cells of aerobic microgranisms
JPS5642580A (en) Apparatus for analyzing enzymic activity
SU697925A1 (en) Method of calibrating polarographic oxygen cell
JPS5461984A (en) Electrochemical measuring method of alcohol concentrations and electrode used for the same
Zhou et al. Development of a microbiosensor for the determination of choline and acetylcholine
JPS5534072A (en) Dialysis membrane bearing fixed enzyme and automatic analysis using the same
JPS55148100A (en) Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide