JPS5664783A - Apparatus for analyzing enzyme activity - Google Patents
Apparatus for analyzing enzyme activityInfo
- Publication number
- JPS5664783A JPS5664783A JP14060979A JP14060979A JPS5664783A JP S5664783 A JPS5664783 A JP S5664783A JP 14060979 A JP14060979 A JP 14060979A JP 14060979 A JP14060979 A JP 14060979A JP S5664783 A JPS5664783 A JP S5664783A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- immobilized enzyme
- detectors
- hydrogen peroxide
- living body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
PURPOSE: To measure the enzyme activity in a living body sample with high accuracy and rapidly, by utilizing the characteristics of an immobilized enzyme, oxygen detectors or hydrogen peroxide detectors.
CONSTITUTION: For measuring the activity of GOT (glutamic acid oxalacetate transaminase), a living body sample injected from a sample inlet 2 of a flow path directed by a pump 11 is passed through the first immobilized enzyme reactor 3 of a pyruvate oxidase with a buffer solution 1 to decompose the pyruvic acid in the sample, and aspartic acid and α-ketoglutaric acid fed from a substrate storage tank 4 are changed into an oxalacetic acid by the GOT in the living body sample. The oxaloacetic acid is then converted into pyruvic acid by an immobilized enzyme reactor 5 of an oxalacetate dehydrocarboxylase. The concentration oxygen or hydrogen peroxide is detected by the first detector 6, and the pyruvic acid in the solution is decomposed by the second immobilized enzyme reactor 7 of a pyruvate oxidase to detect the concentration of oxygen or hydrogen peroxide in the solution by the second detector 8. The output difference between the first and the second detectors 6 and 8 is amplified by an amplifier 9 and recorded in a recorder 10.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14060979A JPS5664783A (en) | 1979-10-30 | 1979-10-30 | Apparatus for analyzing enzyme activity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14060979A JPS5664783A (en) | 1979-10-30 | 1979-10-30 | Apparatus for analyzing enzyme activity |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5664783A true JPS5664783A (en) | 1981-06-02 |
Family
ID=15272680
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP14060979A Pending JPS5664783A (en) | 1979-10-30 | 1979-10-30 | Apparatus for analyzing enzyme activity |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5664783A (en) |
-
1979
- 1979-10-30 JP JP14060979A patent/JPS5664783A/en active Pending
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Rigo et al. | Polarographic determination of superoxide dismutase | |
Nanjo et al. | Enzyme electrode sensing oxygen for uric acid in serum and urine | |
Lippi et al. | A new colorimetric ultramicromethod for serum glutamicoxalacetic and glutamic-pyruvic transaminase determination | |
Watanabe et al. | Determination of phosphate ions with an enzyme sensor system | |
ES8507689A1 (en) | Detection of the presence of biological activity utilizing infrared analysis. | |
Preuschoff et al. | Chemiluminometric L-lysine determination with immobilized lysine oxidase by flow-injection analysis | |
Watanabe et al. | Simultaneous determination of hypoxanthine and inosine with an enzyme sensor | |
Preuschoff et al. | Photodiode-based chemiluminometric biosensors for hydrogen peroxide and L-lysine | |
Lavagnini et al. | Amperometric lysine bioprobes analysis in feeds | |
Cheng et al. | Amperometric measurement of enzyme reactions with an oxygen electrode using air oxidation of reduced nicotinamide adenine dinucleotide | |
JPS5664783A (en) | Apparatus for analyzing enzyme activity | |
JPS57110198A (en) | Determination of choline esterase activity | |
Palleschi et al. | Flow monitoring of glutamate and aspartate in foods and pharmaceutical products with immobilized bienzyme electrochemical cells | |
Macholan | Recent progress in developing enzyme and tissue‐based membrane electrodes | |
IE800647L (en) | Device for measuring a gaseous anhydride | |
JPS5516203A (en) | Measuring method of activity of microbe | |
JPS5664782A (en) | Apparatus for analyzing enzyme activity | |
JPS5758895A (en) | Analytical method of creatinine and apparatus | |
JPS5774095A (en) | Rapid counting of cells of aerobic microgranisms | |
JPS5642580A (en) | Apparatus for analyzing enzymic activity | |
SU697925A1 (en) | Method of calibrating polarographic oxygen cell | |
JPS5461984A (en) | Electrochemical measuring method of alcohol concentrations and electrode used for the same | |
Zhou et al. | Development of a microbiosensor for the determination of choline and acetylcholine | |
JPS5534072A (en) | Dialysis membrane bearing fixed enzyme and automatic analysis using the same | |
JPS55148100A (en) | Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide |