JPS5538318A - Separation and purification of agent inhibiting mutation of mutagen from broccoli juice - Google Patents
Separation and purification of agent inhibiting mutation of mutagen from broccoli juiceInfo
- Publication number
- JPS5538318A JPS5538318A JP11113678A JP11113678A JPS5538318A JP S5538318 A JPS5538318 A JP S5538318A JP 11113678 A JP11113678 A JP 11113678A JP 11113678 A JP11113678 A JP 11113678A JP S5538318 A JPS5538318 A JP S5538318A
- Authority
- JP
- Japan
- Prior art keywords
- juice
- mutation
- broccoli
- mutagen
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
PURPOSE: To separate and purify an inhibitor of mutation having a carcinostatic action, by treating a broccoli juice with an anion exchange resin, and by eluting the adsorbed fraction with a dil. salt solution.
CONSTITUTION: A juice obtained from a washed broccoli containing a substance to inhibit the mutation of mutagens, is treated with an anion exchange resin, e.g. DEAE-cellulose, and the adsorbed fraction is eluted with a dil. salt solution, separated, and purified. The inhibitor thus obtained has characteristic absorption spectra at 280W404nm and a molecular weight of about 55,000, and is stable to heat and inactivated by a proteinase. Preferably, a pretreating step e.g. centrifugation of the juice to obtain a supernatant liquid, is combined with an aftertreating step, e.g. gel filtration and ultrafiltration to improve the effeciency.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11113678A JPS5538318A (en) | 1978-09-08 | 1978-09-08 | Separation and purification of agent inhibiting mutation of mutagen from broccoli juice |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11113678A JPS5538318A (en) | 1978-09-08 | 1978-09-08 | Separation and purification of agent inhibiting mutation of mutagen from broccoli juice |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5538318A true JPS5538318A (en) | 1980-03-17 |
JPS6210968B2 JPS6210968B2 (en) | 1987-03-10 |
Family
ID=14553359
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11113678A Granted JPS5538318A (en) | 1978-09-08 | 1978-09-08 | Separation and purification of agent inhibiting mutation of mutagen from broccoli juice |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5538318A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02204197A (en) * | 1988-12-30 | 1990-08-14 | Ppg Ind Inc | Window panel assembly |
-
1978
- 1978-09-08 JP JP11113678A patent/JPS5538318A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02204197A (en) * | 1988-12-30 | 1990-08-14 | Ppg Ind Inc | Window panel assembly |
Also Published As
Publication number | Publication date |
---|---|
JPS6210968B2 (en) | 1987-03-10 |
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