JPS5538318A - Separation and purification of agent inhibiting mutation of mutagen from broccoli juice - Google Patents

Separation and purification of agent inhibiting mutation of mutagen from broccoli juice

Info

Publication number
JPS5538318A
JPS5538318A JP11113678A JP11113678A JPS5538318A JP S5538318 A JPS5538318 A JP S5538318A JP 11113678 A JP11113678 A JP 11113678A JP 11113678 A JP11113678 A JP 11113678A JP S5538318 A JPS5538318 A JP S5538318A
Authority
JP
Japan
Prior art keywords
juice
mutation
broccoli
mutagen
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11113678A
Other languages
Japanese (ja)
Other versions
JPS6210968B2 (en
Inventor
Kazuyoshi Morita
Hirokazu Yamada
Seiichi Iwamoto
Mikio Tonomura
Akira Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanebo Ltd
Original Assignee
Kanebo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanebo Ltd filed Critical Kanebo Ltd
Priority to JP11113678A priority Critical patent/JPS5538318A/en
Publication of JPS5538318A publication Critical patent/JPS5538318A/en
Publication of JPS6210968B2 publication Critical patent/JPS6210968B2/ja
Granted legal-status Critical Current

Links

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  • Medicines Containing Plant Substances (AREA)

Abstract

PURPOSE: To separate and purify an inhibitor of mutation having a carcinostatic action, by treating a broccoli juice with an anion exchange resin, and by eluting the adsorbed fraction with a dil. salt solution.
CONSTITUTION: A juice obtained from a washed broccoli containing a substance to inhibit the mutation of mutagens, is treated with an anion exchange resin, e.g. DEAE-cellulose, and the adsorbed fraction is eluted with a dil. salt solution, separated, and purified. The inhibitor thus obtained has characteristic absorption spectra at 280W404nm and a molecular weight of about 55,000, and is stable to heat and inactivated by a proteinase. Preferably, a pretreating step e.g. centrifugation of the juice to obtain a supernatant liquid, is combined with an aftertreating step, e.g. gel filtration and ultrafiltration to improve the effeciency.
COPYRIGHT: (C)1980,JPO&Japio
JP11113678A 1978-09-08 1978-09-08 Separation and purification of agent inhibiting mutation of mutagen from broccoli juice Granted JPS5538318A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11113678A JPS5538318A (en) 1978-09-08 1978-09-08 Separation and purification of agent inhibiting mutation of mutagen from broccoli juice

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11113678A JPS5538318A (en) 1978-09-08 1978-09-08 Separation and purification of agent inhibiting mutation of mutagen from broccoli juice

Publications (2)

Publication Number Publication Date
JPS5538318A true JPS5538318A (en) 1980-03-17
JPS6210968B2 JPS6210968B2 (en) 1987-03-10

Family

ID=14553359

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11113678A Granted JPS5538318A (en) 1978-09-08 1978-09-08 Separation and purification of agent inhibiting mutation of mutagen from broccoli juice

Country Status (1)

Country Link
JP (1) JPS5538318A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02204197A (en) * 1988-12-30 1990-08-14 Ppg Ind Inc Window panel assembly

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02204197A (en) * 1988-12-30 1990-08-14 Ppg Ind Inc Window panel assembly

Also Published As

Publication number Publication date
JPS6210968B2 (en) 1987-03-10

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