JPS54122713A - Separation of leukocyte - Google Patents

Separation of leukocyte

Info

Publication number
JPS54122713A
JPS54122713A JP2980078A JP2980078A JPS54122713A JP S54122713 A JPS54122713 A JP S54122713A JP 2980078 A JP2980078 A JP 2980078A JP 2980078 A JP2980078 A JP 2980078A JP S54122713 A JPS54122713 A JP S54122713A
Authority
JP
Japan
Prior art keywords
leukocyte
erythrocyte
blood
filter
capturing material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2980078A
Other languages
Japanese (ja)
Other versions
JPS5854130B2 (en
Inventor
Toru Kuroda
Nobuaki Tsuda
Yoshinori Takenaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd filed Critical Asahi Chemical Industry Co Ltd
Priority to JP53029800A priority Critical patent/JPS5854130B2/en
Publication of JPS54122713A publication Critical patent/JPS54122713A/en
Publication of JPS5854130B2 publication Critical patent/JPS5854130B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To separate high purity leukocyte containing limited amount of erythrocyte, blood platelet, protein, etc., in high yield, by treating blood or body fluid with a leukocyte-capturing material or 9 filter to capture the leukocyte, and treating the captured leukocyte with a hemolyzing agent.
CONSTITUTION: Blood or body fluid such as cerebrospinal fluid, lymph, etc., or a cell dispersion obtained by removing most part of erythrocyte from the blood, etc., is treated with a leukocyte-capturing material such as nylon fiber, glass beads, etc., or a leukocyte-separating filter having a mesh size of about 5μ. The capturing material or the filter containing captured leukocyte is treated with a hemolyzing agent such as 0.25W0.35% NaCl solution to disintegrate the erythrocyte, and washed with a heparin-containing physiological saline solution. A dispersion of leukocyte containing less than several % of erythrocyte in number can be prepared by this process.
COPYRIGHT: (C)1979,JPO&Japio
JP53029800A 1978-03-17 1978-03-17 Leukocyte separation method Expired JPS5854130B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53029800A JPS5854130B2 (en) 1978-03-17 1978-03-17 Leukocyte separation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53029800A JPS5854130B2 (en) 1978-03-17 1978-03-17 Leukocyte separation method

Publications (2)

Publication Number Publication Date
JPS54122713A true JPS54122713A (en) 1979-09-22
JPS5854130B2 JPS5854130B2 (en) 1983-12-02

Family

ID=12286073

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53029800A Expired JPS5854130B2 (en) 1978-03-17 1978-03-17 Leukocyte separation method

Country Status (1)

Country Link
JP (1) JPS5854130B2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4416777A (en) * 1979-10-09 1983-11-22 Asahi Kasei Kogyo Kabushiki Kaisha Separation of leukocytes or lymphocytes from leukocyte-containing suspension
US4836928A (en) * 1984-04-28 1989-06-06 Terumo Kabushiki Kaisha Separation method, separation device and separation apparatus for separating body fluid into respective components
JP2006160731A (en) * 2004-11-11 2006-06-22 Kuraray Medical Inc Blood ingredient-recovering apparatus
WO2019207724A1 (en) * 2018-04-26 2019-10-31 株式会社ニコン Blood component separation device, blood component separation method, and blood component analysis method
JP2021504712A (en) * 2017-11-30 2021-02-15 ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド Methods, systems and filtration units for isolating particles from biological samples

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230090675A1 (en) * 2020-03-11 2023-03-23 Sekisui Medical Co., Ltd. Leukocyte concentration separation device, blood collection container, and method for separating leukocytes

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4416777A (en) * 1979-10-09 1983-11-22 Asahi Kasei Kogyo Kabushiki Kaisha Separation of leukocytes or lymphocytes from leukocyte-containing suspension
US4836928A (en) * 1984-04-28 1989-06-06 Terumo Kabushiki Kaisha Separation method, separation device and separation apparatus for separating body fluid into respective components
JP2006160731A (en) * 2004-11-11 2006-06-22 Kuraray Medical Inc Blood ingredient-recovering apparatus
JP2021504712A (en) * 2017-11-30 2021-02-15 ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド Methods, systems and filtration units for isolating particles from biological samples
WO2019207724A1 (en) * 2018-04-26 2019-10-31 株式会社ニコン Blood component separation device, blood component separation method, and blood component analysis method
JPWO2019207724A1 (en) * 2018-04-26 2021-01-14 株式会社ニコン Blood component separation device, blood component separation method, and blood component analysis method

Also Published As

Publication number Publication date
JPS5854130B2 (en) 1983-12-02

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