JPS54122713A - Separation of leukocyte - Google Patents
Separation of leukocyteInfo
- Publication number
- JPS54122713A JPS54122713A JP2980078A JP2980078A JPS54122713A JP S54122713 A JPS54122713 A JP S54122713A JP 2980078 A JP2980078 A JP 2980078A JP 2980078 A JP2980078 A JP 2980078A JP S54122713 A JPS54122713 A JP S54122713A
- Authority
- JP
- Japan
- Prior art keywords
- leukocyte
- erythrocyte
- blood
- filter
- capturing material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To separate high purity leukocyte containing limited amount of erythrocyte, blood platelet, protein, etc., in high yield, by treating blood or body fluid with a leukocyte-capturing material or 9 filter to capture the leukocyte, and treating the captured leukocyte with a hemolyzing agent.
CONSTITUTION: Blood or body fluid such as cerebrospinal fluid, lymph, etc., or a cell dispersion obtained by removing most part of erythrocyte from the blood, etc., is treated with a leukocyte-capturing material such as nylon fiber, glass beads, etc., or a leukocyte-separating filter having a mesh size of about 5μ. The capturing material or the filter containing captured leukocyte is treated with a hemolyzing agent such as 0.25W0.35% NaCl solution to disintegrate the erythrocyte, and washed with a heparin-containing physiological saline solution. A dispersion of leukocyte containing less than several % of erythrocyte in number can be prepared by this process.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53029800A JPS5854130B2 (en) | 1978-03-17 | 1978-03-17 | Leukocyte separation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53029800A JPS5854130B2 (en) | 1978-03-17 | 1978-03-17 | Leukocyte separation method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54122713A true JPS54122713A (en) | 1979-09-22 |
JPS5854130B2 JPS5854130B2 (en) | 1983-12-02 |
Family
ID=12286073
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53029800A Expired JPS5854130B2 (en) | 1978-03-17 | 1978-03-17 | Leukocyte separation method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5854130B2 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4416777A (en) * | 1979-10-09 | 1983-11-22 | Asahi Kasei Kogyo Kabushiki Kaisha | Separation of leukocytes or lymphocytes from leukocyte-containing suspension |
US4836928A (en) * | 1984-04-28 | 1989-06-06 | Terumo Kabushiki Kaisha | Separation method, separation device and separation apparatus for separating body fluid into respective components |
JP2006160731A (en) * | 2004-11-11 | 2006-06-22 | Kuraray Medical Inc | Blood ingredient-recovering apparatus |
WO2019207724A1 (en) * | 2018-04-26 | 2019-10-31 | 株式会社ニコン | Blood component separation device, blood component separation method, and blood component analysis method |
JP2021504712A (en) * | 2017-11-30 | 2021-02-15 | ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド | Methods, systems and filtration units for isolating particles from biological samples |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20230090675A1 (en) * | 2020-03-11 | 2023-03-23 | Sekisui Medical Co., Ltd. | Leukocyte concentration separation device, blood collection container, and method for separating leukocytes |
-
1978
- 1978-03-17 JP JP53029800A patent/JPS5854130B2/en not_active Expired
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4416777A (en) * | 1979-10-09 | 1983-11-22 | Asahi Kasei Kogyo Kabushiki Kaisha | Separation of leukocytes or lymphocytes from leukocyte-containing suspension |
US4836928A (en) * | 1984-04-28 | 1989-06-06 | Terumo Kabushiki Kaisha | Separation method, separation device and separation apparatus for separating body fluid into respective components |
JP2006160731A (en) * | 2004-11-11 | 2006-06-22 | Kuraray Medical Inc | Blood ingredient-recovering apparatus |
JP2021504712A (en) * | 2017-11-30 | 2021-02-15 | ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド | Methods, systems and filtration units for isolating particles from biological samples |
WO2019207724A1 (en) * | 2018-04-26 | 2019-10-31 | 株式会社ニコン | Blood component separation device, blood component separation method, and blood component analysis method |
JPWO2019207724A1 (en) * | 2018-04-26 | 2021-01-14 | 株式会社ニコン | Blood component separation device, blood component separation method, and blood component analysis method |
Also Published As
Publication number | Publication date |
---|---|
JPS5854130B2 (en) | 1983-12-02 |
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