JPH11304672A - Standard counter plate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To delay the evaporation of water content in a liquid sample which is observed with a microscope. SOLUTION: A microscopy chamber 4 wherein, sandwiched between transparent bodies, a gap width in vertical direction causes capillarity, and a plurality of holes 6 and 6a which communicate the microscopy chamber 4 with outside, are provided. Here, a plurality of auxiliary chambers 5 and 5a smaller than the microscopy chamber 4 are provided for communication in it while holes 6 and 6a for communication with outside are opened in the auxiliary chamber 5 at a position away from the microscopy chamber, so that evaporation of water content in the sample in the auxiliary chambers 5 and 5a delay the evaporation of water content in the sample in the microscopy chamber 4.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a standard counting plate for a liquid sample when performing a water quality test on rivers, lakes, marshes, oceans, and the like, and a clinical test such as a blood test and a urine test by microscopic observation.

[0002]

2. Description of the Related Art Conventionally, an observation plate shown in FIGS. 4 and 5 has been proposed (JP-A-63-19532). This is one in which a frame (71) partitioned into a plurality of rooms is bonded and interposed between the slide glass (7) and the cover glass (72), and between the slide glass (7) and the cover glass (72). Multiple speculum rooms
(73) (73) is formed, and both ends of each microscopic room (73) are large and small window holes
(74) It communicates with the outside by (75).

When a liquid sample is dropped onto the slide glass (7) from the large window (74), the sample is subject to capillary action to cause the speculum chamber (7) between the slide glass (7) and the cover glass (72).
Invade 3). The air in the speculum room (73) is exhausted from the small window (75).

[0004]

The observation plate is composed of a slide glass (7), a frame (71) and a cover glass (7).
Since 2) is integrally formed by bonding, it is easier to handle and workability is improved as compared with the case where a cover glass (72) is put on a sample dropped on a slide glass (7), which was performed before that. I do. In addition, although the cover glass (72) was not inadvertently damaged, the following problems were found.

That is, since the speculum chamber (73) is in direct communication with the holes (74) and (75) and the water in the sample is easily evaporated, the speculum is evaporated by the evaporation of water after a lapse of time after the sample injection. Air enters the room (73), preventing correct inspection and observation. The present invention clarifies a standard counting plate that can solve the above-mentioned problem.

[0006]

A standard counting plate according to the present invention comprises a speculum chamber (4) sandwiched between transparent bodies and having a vertical gap width in which a capillary phenomenon may occur, and a communication between the speculum chamber (4) and the outside. Multiple holes
(6) In the standard counting plate having (6a), a plurality of auxiliary chambers (5) and (5a) smaller than the microscopic chamber (4) communicate with the speculum chamber (4), and the auxiliary chamber (5) communicates with the auxiliary chamber (5). Holes (6) and (6a) for communication with the outside are opened at a position away from the speculum room (4).

[0007]

When a liquid sample is dropped onto the transparent substrate (1) from the hole (6), the sample enters the speculum room (4) via the auxiliary room (5) by capillary action. The air in the speculum room (4) is discharged from the hole (6a) through the other auxiliary room (5a).
(4) The sample can be quickly filled. Therefore, correct inspection and observation can be performed with a microscope.

Since the holes (6) and (6a) for communicating with the outside are opened in the auxiliary chambers (5) and (5a) at positions away from the speculum chamber (4), evaporation of the water of the sample is performed in the auxiliary chamber (5). Start with the sample filled in (5a). For this reason, there is a time margin until the water content of the specimen in the speculum chamber (4) evaporates, and the time restriction that the inspection and observation must be performed immediately after the specimen injection is released.

[0009]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS As shown in FIG.
A transparent substrate (1) on the lower surface of the transparent frame plate (2) and a transparent sheet on the upper surface
(3) is bonded in a liquid-tight manner to form a rectangle having a length of about 75 mm and a width of about 25 mm and a thickness of about 1.8 mm.

In the embodiment, a transparent substrate (1), a transparent frame plate (2)
The transparent sheet (3) is formed of a biodegradable resin to facilitate disposal, but is not limited thereto and may be formed of a general synthetic resin or glass. In addition, the frame plate (2)
Need not necessarily be transparent. The transparent frame plate (2) and the transparent sheet (3) can be integrally molded, or the transparent frame plate (2) and the transparent substrate (1) can be integrally molded.

In the embodiment, a speculum room described later on the transparent substrate (1) is used.
In the position corresponding to (4), within a square of 10 mm x 10 mm,
Lines (11) (field lines) are shown in a grid pattern at a pitch of 0.5 mm or at a pitch of 1.0 mm. Line (11) is the transparent substrate (1)
Is raised on the narrow ridge or recessed in the narrow groove, or is represented by printing.

The frame plate (2) is provided with a plurality of through holes (21) serving as a speculum room (4) and auxiliary rooms (5) and (5a), which will be described later.
~ 3 have been established. Each through hole (21) has a diameter of about 15 mm
Medium diameter round hole (21b) on both sides of large diameter round hole (21a)
(21b) A small-diameter round hole (21c) having a diameter of about 3 mm is formed outside each medium-diameter round hole (21b) so as to partially overlap each other and communicate with each other. 21c) (21c), medium diameter round hole (2
1b) and (21b) and the large-diameter round hole (21a) are arranged in a straight line such that the center is located at an imaginary line L at an angle of 45 ° with respect to the longitudinal direction of the frame plate (2). Adjacent through holes (21) (21) do not overlap each other independently.

The boundary between the small-diameter round hole (21c) and the medium-diameter round hole (21b) and the boundary between the medium-diameter round hole (21b) and the large-diameter round hole (21a) are narrowed and narrow.

In the transparent sheet (3), holes (6) (6a) having substantially the same diameter as the small-diameter round holes (21c) are provided at positions corresponding to the small-diameter round holes (21c) of the frame plate (2). Has been established.

FIG. 2 shows a part of a plan view of a standard counting plate,
FIG. 3 shows a sectional view along the line AA in FIG. The bottom of the through hole (21) of the frame plate (2) is closed by a transparent substrate (1), and the ceiling of the large-diameter round hole (21a) and the medium-diameter round hole (21b) is covered by a transparent sheet (3). The ceiling of the small-diameter round hole (21c) is closed, and the holes (6) and (6a) of the transparent sheet (3) are open to the outside correspondingly.

The large-diameter round hole (21a) has a medium-diameter round hole (21a).
b) is the main auxiliary room (51), the small round hole (21c) is the front room (52),
The auxiliary chambers (5) and (5a) are formed by the main auxiliary chamber (51) and the front chamber (52). The gap width between the transparent substrate (1) and the transparent sheet (3) in the speculum chamber (4) and the auxiliary chambers (5) and (5a) is not less than 0.5 mm and not more than 2 mm. The dimensions do not matter.

However, when a liquid sample is dropped onto the transparent substrate (1) from one of the holes (6), the sample enters the speculum chamber (4) via the auxiliary chamber (5) by capillary action. Since the air in the speculum chamber (4) is discharged from the hole (6a) through the other auxiliary chamber (5a), there is no problem in filling the speculum chamber (4) with the sample.

Since the holes (6) and (6a) are opened in the auxiliary chambers (5) and (5a) at positions away from the speculum chamber (4), evaporation of the moisture of the sample is prevented in the auxiliary chambers (5) and (5a). To start with a full sample,
There is time to evaporate the water content of the specimen in the speculum room (4), and the time restriction that inspection and observation must be performed immediately after the specimen injection is released.

As in the embodiment, the speculum room (4) and the auxiliary room (5)
On the lower and upper surfaces of the frame plate (2) having the through holes (21)
The transparent substrate (1) and the transparent sheet (3) are adhered to close the through hole (21), and the transparent sheet (3) is provided with holes (6) and (6a) for communication with the outside. Can be manufactured efficiently and at low cost.

As in the embodiment, a main auxiliary room (51) connected to the speculum room (4) and a front room (52) communicating with the main auxiliary room (51) and smaller than the main auxiliary room (51). The auxiliary chamber (5) is constituted by
If a hole (6) (6a) for communicating with the outside is opened at a position corresponding to (52), evaporation of the moisture of the sample is prevented by the front chamber (52) of the main auxiliary chamber (51).
Since it starts from the side, the water evaporation of the specimen in the speculum room (4) can be more reliably delayed.

Further, as in the embodiment, the speculum room (4) and each auxiliary room are provided.
(5) By narrowing the boundary between (5) and (5a) and narrowing the flow passage between the two chambers, it is possible to further delay evaporation of water in the speculum chamber (4).

Further, by providing a plurality of independent speculum chambers (4) on one standard counting plate, a plurality of specimens can be observed with a microscope. On the transparent substrate (1), corresponding to the speculum room (4),
Since the lines (11) (boundary lines) are shown vertically and horizontally in a grid pattern, the sample can be quantified. Further, the position of the specimen can be specified.
The line (11) may be a parallel line at equal intervals in the vertical or horizontal direction. Also, the line (11) may be represented on the transparent sheet (3) instead of the transparent substrate (1).

The present invention is not limited to the configuration of the above embodiment, and various modifications can be made within the scope of the claims.

[Brief description of the drawings]

FIG. 1 is an exploded perspective view of a standard counting plate.

FIG. 2 is a plan view in which a part of a standard counting plate is cut away.

FIG. 3 is a sectional view taken along line AA of FIG. 2;

FIG. 4 is a perspective view of a conventional observation plate.

FIG. 5 is a sectional view taken along line BB of FIG. 4;

[Explanation of symbols]

 (1) Transparent substrate (2) Frame plate (3) Transparent sheet (4) Microscope room (5) Auxiliary room (6) Hole

 ──────────────────────────────────────────────────の Continuing on the front page (72) Koji Noguchi, Inventor Kojiwada-shi, Osaka, 165, Katsumatsu-cho, Matsunami Glass Industry Co., Ltd.

Claims (5)

[Claims] 1. A speculum chamber (4) in which a gap in the vertical direction is sandwiched between transparent bodies and in which a capillary phenomenon may occur, and a plurality of holes (6) (6a) for communicating the speculum chamber (4) with the outside. In the standard counting plate having the above, the speculum room (4) has a plurality of auxiliary rooms smaller than the room.
(5) A standard counter plate in which the auxiliary chamber (5) communicates and the auxiliary chamber (5) has holes (6) and (6a) for communicating with the outside at a position away from the speculum room. 2. A transparent substrate is provided on a lower surface and an upper surface of a frame plate (2) having a through hole (21) serving as a speculum room (4) and an auxiliary room (5) (5a).
The transparent sheet (3) is adhered to (1) to close the through hole (21), and the transparent sheet (3) is provided with holes (6) (6a) for communication with the outside. Standard counter plate. 3. Each of the auxiliary chambers (5) and (5a) has a main auxiliary room (51) connected to the speculum room (4) and a main auxiliary room (5) communicating with the main auxiliary room (51).
3. A front room (52) smaller than 1), wherein holes (6) and (6a) for communication with the outside are opened at positions corresponding to the front room (52). Standard counter plate. 4. The method according to claim 1, wherein a boundary between the speculum chamber (4) and each of the auxiliary chambers (5) and (5a) is narrowed, and a flow passage between the two chambers is narrower than the two chambers. The standard counting plate according to any of the above. 5. The transparent substrate (1) or the transparent sheet (3) includes:
The standard counting plate according to any one of claims 1 to 4, wherein a line (11) is displayed in a unit area at a position corresponding to the speculum room (4) in the form of parallel lines or grids.