JPH11248623A - Spectrophotometer and automatic analyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an automatic analyzer capable of making absorbance spectrophotometric measurements and spectrofluorometric measurements by itself. SOLUTION: In this spectrophotometer, fluorescence of the measured an object to be measured 18 energized by a high-luminance light source 13 is condensed on an absorbance spectrophotometric slit 20 by a condenser mirror 21, transmitted light is dispersed into a spectrum by a spectral element 22, and sectrofluorometric measurements are made by selectively detecting light with a target wave length by a photo detector 23a. Light from a light source 24 for absorbance spectrophotometric measurement is permeated through the measured object 18, is condensed on a absorbance spectral slit 20, spectral separation of transmitted light is made by the spectral element 22 and absorbance spectrophotometric measurements are made by selectively detecting light with a target wave length by a photo detector 23b.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a so-called automatic analyzer for automatically analyzing chemical components contained in human blood and urine, and more particularly to a spectrophotometer mounted on the automatic analyzer.

[0002]

2. Description of the Related Art A conventional fluorescence spectroscopic measurement technique and its optical system are described in “Applied Spectroscopy Handbook” edited by Hiroshi Yoshinaga (Asakura Shoten, 1973), pp. 693 to 712. Conventional automatic analyzers are, for example, USP 4,451,433 (Auto
As described in matic chemical analyzer), absorbance spectroscopy measures the biochemical properties of blood and urine.

[0003]

As a countermeasure for the case of shock symptoms due to cardiac arrest or a decrease in blood pressure during surgery, catecholamines (adrenaline, noradrenaline, dopamine) are administered to the patient. At the time of follow-up after administration, the concentrations of these substances in the blood are measured by fluorescence spectroscopy. At the same time, it is necessary to simultaneously measure blood glucose, glucose, urea nitrogen, amylase, ammonia, and bilirubin by absorbance spectrometry.

[0004] The optical system of a fluorescence spectrophotometer measures the amount of fluorescence emitted from a specimen, while the optical system of an absorbance spectrophotometer absorbs a part of light from a light source. There is a big difference in measuring the amount that you do.

[0005] Therefore, for example, in order to cope with an urgent examination during surgery, it is necessary to separately install a fluorescence spectrophotometer and an absorbance spectrophotometer and measure them separately. However, there are problems such as a large amount of blood collected, a high cost of the apparatus, and a large installation space.

An object of the present invention is to provide an automatic analyzer capable of performing absorbance spectrophotometry and fluorescence spectrophotometry with one device.

[0007]

SUMMARY OF THE INVENTION In order to achieve the above object, the present invention provides a spectrophotometer for an automatic analyzer for automatically analyzing chemical components contained in human blood or urine. , A high-intensity light source for fluorescence spectrometry, an optical system for selecting light of a predetermined wavelength from the light source and condensing the light on the cell, and an optical system arranged in the cell adjacent to the cell and excited in the cell. A condenser mirror for condensing the fluorescence of the measurement sample, a slit for absorbance spectrometry for passing the condensed fluorescence, a spectroscopic element for dispersing the fluorescence transmitted through the slit for absorbance spectrometry, and A photodetector array that selectively detects light of the target wavelength and measures fluorescence spectroscopy, a light source for absorbance spectroscopy, and a light source of a predetermined wavelength selected from the light source for absorbance spectroscopy and transmitted through the analyte to be measured for absorbance spectroscopy For slip An optical system that focuses light on the top, a photodetector array that selectively detects light of the target wavelength separated by the spectroscopic element and measures absorbance spectroscopy, and a light from a high-intensity light source for fluorescence spectrometry and absorbance spectrometry A spectrophotometer provided with switching means for selectively irradiating a cell with light from a light source.

[0008] The condenser mirror is inserted into an adjacent cell /
An extraction mechanism may be provided, and may be fixedly installed in every other cell of the reaction tank.

The present invention also relates to a spectrophotometer for an automatic analyzer for automatically analyzing chemical components contained in human blood and urine, etc. A luminance light source, an optical system for selecting light of a predetermined wavelength from the light source and condensing the light on a cell, and a condensing light for concentrating fluorescent light of a specimen to be measured which is fixedly arranged at a position adjacent to the cell and excited in the cell. A mirror, a slit for absorbance spectrometry for passing the collected fluorescent light, a spectroscopic element for splitting the fluorescent light transmitted through the slit for absorbance spectroscopic measurement, and a screen for selectively detecting the light of the target wavelength that has been split. A photodetector array for measuring optical spectroscopy, a light source for absorbance spectrometry, and an optical system for selecting light of a predetermined wavelength from the light source for absorbance spectrometry, transmitting the light to the analyte, and condensing it on the slit for absorbance spectrometry, Spectroscopy Photodetector array that selectively detects light of the target wavelength separated by the photodetector and measures absorbance spectroscopy, and selectively uses light from a high-intensity light source for fluorescence spectroscopy and light from a light source for absorbance spectroscopy A spectrophotometer including a switching unit for irradiating a cell is proposed.

In order to achieve the above object, the present invention further provides a sampler for holding a sample such as human blood or urine, a sample pipetter for collecting a sample, a spectrophotometer, a reaction tank, and a cleaning mechanism. And, a stirring mechanism, a reagent pipettor, a reagent bottle, and a plurality of reagent tanks, in an automatic analyzer for analyzing the chemical components contained in the sample, equipped with any one of the above spectrophotometer as a spectrophotometer We propose an automatic analyzer.

[0011]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Next, referring to FIGS.
An embodiment of the automatic analyzer according to the present invention will be described.

FIG. 1 is a view schematically showing the appearance of an embodiment of the automatic analyzer according to the present invention. On the upper surface of the automatic analyzer 1, an operation surface 2 of the automatic analyzer is provided. A sampler 3 for holding a sample, a sample pipetter 4 for collecting a sample, a spectrophotometer 5, a reaction tank 6, a cleaning mechanism 7, And stirring mechanism 8
, A reagent pipettor 9, a reagent bottle 10, a first reagent tank 11, and a second reagent tank 12 are arranged. The basic functions of these components are as described in, for example, paragraphs 104 to 116 of "Automated Measurement Method in Blood Test" (edited by Fumio Yoshino and Hisao Osawa, Gakkai Shuppan Center, 1986). Here, the description is omitted. The invention relates to the part of the spectrophotometer 5 in such an automatic analyzer.

<First Embodiment> FIG. 2 is a perspective view showing a system configuration in a case where a first embodiment of a spectrophotometer according to the present invention is used for fluorescence spectrometry. Light from the high-intensity light source 13 is condensed by a condenser lens 14 through a transmission window 17a attached to the reaction vessel 6 to a cell 16a. At this time, the wavelength of the condensed light is selected by the filter 15. For example, when measuring catecholamine, a xenon lamp is used as the high-brightness light source 13 and a filter that selectively transmits a wavelength of 285 nm is used as the filter 15. The substance contained in the test sample 18 held in the cell 16a is excited by the light condensed in the cell 16a to generate fluorescence. On the other hand, the light transmitted through the test sample 18 passes through the transmission window 17 b and is extinguished by the beam stopper 19. Next, the fluorescent light generated in the sample 18 to be measured is passed through the transmission window 17d using the condenser mirror 21 inserted into the cell 16b located immediately before the slit 20 for absorbance spectroscopic measurement, and is subjected to absorbance spectroscopy. The light is focused on the measurement slit 20. The fluorescence transmitted through the slit 20 for absorbance spectrometry is converted into the light by the spectral element 2.
When the light intensity is measured by using the photodetector 23a separately set for each measurement wavelength on the photodetector array 23 and a part of the dispersed light is dispersed by the light from the photodetector 2, fluorescence spectrometry can be performed. For example, when measuring catecholamine, the photodetector array 23a is configured to selectively detect a wavelength of 325 nm.
Place on top.

FIG. 3 is a perspective view showing a system configuration when the optical system of the spectrophotometer of FIG. 2 is used for absorbance spectrometry. The light from the light source 24 for absorbance spectrometry passes through the shutter 25 that is kept open, passes through the transmission window 17c attached to the reaction tank 6 by the condenser lens 26, and passes through the cell 16
b, further passes through the transmission window 17d, and condenses on the slit 20 for absorbance spectrometry. At this time, cell 1
Light having a partial wavelength of transmitted light is absorbed by the measured object held in 6b. Slit 20 for absorbance spectrometry
The light transmitted through the photodetector array 2 is separated by the spectroscopic element 22 and a part of the dispersed light is individually separated for each measurement wavelength.
When the light intensity is measured using the photodetector 23b installed on the light-receiving unit 3, the absorbance spectrometry can be performed. For example, when measuring bilirubin, a halogen lamp is used as the light source 24 for absorbance spectrometry, and the photodetector 2 is used.
3b is installed on the photodetector array 23 so as to selectively detect a wavelength of 450 nm in order to measure the absorption of bilirubin-dependent light.

FIG. 4 is a diagram showing the procedure of fluorescence spectrometry and absorbance spectrometry using the embodiment 1 of FIG. 2 or 3 of the present invention. Timing 1: The emission of the high-intensity light source 13 for fluorescence spectrometry is stopped for the cell 16b located immediately before the slit 20 for absorbance spectrometry in order to perform spectrophotometry. Then, the light source 24 for absorbance spectrometry is kept in a light emitting state, and the shutter 25 is opened. Further, the absorbance spectrometry is performed with the condensing mirror for fluorescence spectrometry pulled up from the cell 16b. Timing 2: The condenser mirror 21 is inserted into the cell 16b. Timing 3: The high-intensity light source for fluorescence spectrometry 13 emits light to perform fluorescence spectrometry on the cell 16a located next to the cell 16b. Light source 24 for absorbance spectrometry
Generally, in order to stabilize the operation, the shutter 25 is closed so that the light does not enter the slit 20 and the fluorescence spectrometry is performed. Timing 4: The focusing mirror 21 is pulled up from the cell 16b. Timing 5: The reaction tank 6 is rotated to move the entire cell. In FIG. 2, 16a moves in the direction of 16b. Timing 6: The emission of the high-intensity light source 13 for fluorescence spectrometry is stopped in order to perform the absorbance spectrometry on the cell 16a positioned immediately before the slit 20 for absorbance spectrometry. Then, the light source 24 for absorbance spectrometry is kept in a light emitting state, the shutter 25 is opened, and the absorbance spectrometry is performed. Timing 7: The condenser mirror 21 is inserted into the cell 16a. Timing 8: In order to perform fluorescence spectrometry on the cell 16c located next to the cell 16a, the fluorescence spectrometry high-intensity light source 13 is caused to emit light, the absorbance spectrometry light source 24 is continuously turned on, and the shutter 25 is turned on. Is closed so that the light does not enter the slit 20, and fluorescence spectrometry is performed. Timing 9: The condenser mirror 21 is pulled up from the cell 16a. Timing 10: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

FIG. 5 is a diagram showing a procedure of only fluorescence spectrometry using the embodiment 1 of FIG. 2 or 3 of the present invention. Timing 1: In order to perform fluorescence spectrometry on the cell 16a, the high-intensity light source 13 for fluorescence spectrometry is caused to emit light while the light collecting mirror 21 is inserted into the cell 16b, and the light source 24 for absorbance spectrometry is stopped ( (The light is turned off) and the fluorescence spectrometry is performed. It should be noted that, instead of stopping the light emission, while the light source 24 for absorbance spectrometry is allowed to emit light,
The same is true even when the shutter 25 is closed. Timing 2: The focusing mirror 21 is pulled up from the cell 16b. Timing 3: The reaction tank 6 is rotated to move the entire cell. In FIG. 2, 16a moves in the direction of 16b. Timing 4: The condenser mirror 21 is inserted into the cell 16a. Timing 5: In order to perform fluorescence spectrometry on the cell 16c, the high-intensity light source 13 for fluorescence spectrometry is caused to emit light,
The light source 24 for absorbance spectrometry is turned off (turned off), and the condenser mirror 21 is inserted into the cell 16a to execute the fluorescence spectrometry. Timing 6: The condenser mirror 21 is pulled up from the cell 16a. Timing 7: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

FIG. 6 is a diagram showing a procedure of only the absorbance spectrometry using the embodiment 1 of FIG. 2 or 3 of the present invention.
At this time, the condenser mirror 21 is held while being raised. Timing 1: In order to perform the absorbance spectrometry on the cell 16b, the light source 24 for the absorbance spectrometry is caused to emit light, the light emission of the high-intensity light source 13 for the fluorescence spectrometry is stopped (the light is turned off), and the absorbance spectrometry is executed. Timing 2: The reaction tank 6 is rotated to move the entire cell. In FIG. 3, 16a moves in the direction of 16b. Timing 3: In order to perform the absorbance spectrometry on the cell 16a, the cell is kept in the previous state (the high-intensity light source 13 for fluorescence spectrometry is stopped emitting while the light source 24 for absorbance spectrometry is kept emitting), and the absorbance spectrometry is performed. Execute Timing 4: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

As described above, according to Embodiment 1 of FIG. 2 or FIG. 3 of the present invention, absorbance spectroscopy and fluorescence spectroscopy can be performed by one automatic analyzer, and the conventional absorbance spectroscopy As compared with the case where the apparatus and the fluorescence spectrometer are respectively installed, the amount of inspection work can be reduced, the apparatus installation scale can be reduced, the amount of blood collected from the patient can be reduced, the burden on the patient can be reduced, and the apparatus cost can be reduced. .

Further, in the fluorescence spectrometry, when the concentration of the component to be measured in the specimen to be measured is high and the concentration quenching of the fluorescence is foreseen, the measurement is switched from the fluorescence spectrometry to the absorbance spectrometry, and accurate measurement is performed. Can measure.

In the first embodiment shown in FIG. 2 or FIG. 3, instead of inserting or pulling out the condenser mirror 21,
It will be clear that the reaction tank 6 in which the condensing mirror 21 is fixedly installed in every other cell may be used. Depending on the case, it is also possible to have two or more.

<Embodiment 2> FIG. 7 is a perspective view showing a system configuration when a spectrophotometer according to Embodiment 2 of the present invention is used for fluorescence spectrometry. Light from the high-intensity light source 13 is condensed by a condenser lens 14 through a transmission window 17a attached to the reaction tank 6 to a cell 16c. At this time, the wavelength of the condensed light is selected by the filter 15. For example, when measuring catecholamine, a xenon lamp and a filter 15 that selectively transmits a wavelength of 285 nm are used as the high-brightness light source 13. The substance contained in the sample to be measured held in the cell 16c is excited by the light condensed in the cell 16c to generate fluorescence. However, the light transmitted through the sample to be measured passes through the transmission window 17b and passes through the beam stopper 1
The light is extinguished at 9. The fluorescence generated by the sample to be measured is
The transmission window 17 is formed by the condensing mirror 30c installed adjacent to 6c.
The light is condensed on the fluorescence spectrometry slit 31 through e. Fluorescence transmitted through the fluorescence spectrometry slit 31 is separated by the spectroscopic element 22, and a part of the dispersed light is reduced in light intensity by using a photodetector 32 a individually provided for each measurement wavelength on a photodetector array 32. Once measured, fluorescence spectrometry can be performed. For example, when measuring catecholamine, the photodetector 32a
Is installed on the photodetector array 32 so as to selectively detect a wavelength of 325 nm. At this time, the fluorescence spectrometry slit 31 and the photodetector 32a are arranged on the Rowland circle of the spectroscopic element 22.

FIG. 8 is a perspective view showing a system configuration when the optical system of the spectrophotometer of FIG. 7 is used for absorbance spectrometry. The light from the light source 24 for absorbance spectrometry passes through the shutter 25 in an open state, and passes through the transmission window 17 c attached to the reaction tank 6 by the condenser lens 26, so that the cell 16
b, and further condensed on the slit 20 for absorbance spectrometry through the transmission window 17d. At this time, cell 1
Light having a partial wavelength of transmitted light is absorbed by the measured object held in 6b. Absorbance spectrometry slit 2
The light transmitted through 0 is split by the spectroscopic element 22, and a part of the dispersed light is separated by using a photodetector 23b individually set for each measurement wavelength on the photodetector array 23.
Once the light intensity is measured, absorbance spectroscopy can be performed. For example, when measuring bilirubin, a halogen lamp is used as the light source 24 for absorbance spectrometry, and the photodetector 23b measures the absorption of bilirubin-dependent light. 23.

FIG. 9 is a diagram showing the procedure of fluorescence spectroscopy and absorbance spectroscopy using the embodiment 2 of FIG. 7 or 8 of the present invention. Timing 1: The high-intensity light source 13 for fluorescence spectrometry stops emitting light in order to perform spectrophotometry on the cell 16b located immediately before the slit 20 for spectrophotometry. Then, the absorbance spectrometry is performed with the light source for absorbance spectrometry 24 in a light emitting state and the shutter 25 in an open state. Timing 2: To perform fluorescence spectrometry on the cell 16c located adjacent to the cell 16b, the high-intensity light source 13 for fluorescence spectrometry is caused to emit light, and the light source 24 for absorbance spectrometry is kept in a continuous lighting state. Close 25,
Fluorescence spectrometry is performed so that the light does not enter the slit 20. Timing 3: The reaction tank 6 is rotated to move the entire cell. In FIG. 7, 16c moves in the direction of 16b. Timing 4: The high-intensity light source 13 for fluorescence spectrometry is used to perform spectrophotometry on the cell 16c positioned immediately before the slit 20 for spectrophotometry.
The light emission is stopped. The light source 24 for absorbance spectrometry is in a light emitting state, and the shutter 25 is in an open state to execute the absorbance spectrometry. Timing 5: For the cell 16f located next to the cell 16c, the fluorescence spectrometry high-intensity light source 13 emits light and the absorbance spectrometry measurement light source 24
In order to keep the device in the continuous lighting state, the shutter 25 is closed so that the light does not enter the slit 20 and the fluorescence spectrometry is performed. Timing 6: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

FIG. 10 is a diagram showing the procedure of only the fluorescence spectrometry using the embodiment 2 of FIG. 7 or 8 of the present invention. Timing 1: In order to perform fluorescence spectrometry on the cell 16c, the fluorescence spectrometry high-intensity light source 13 is caused to emit light,
The light source 24 for absorbance spectrometry is stopped from emitting light (turned off), and fluorescence spectrometry is performed. Note that the same applies to the case where the shutter 25 is closed while the light source 24 for absorbance spectrometry is emitting light instead of stopping the light emission. Timing 2: The reaction tank 6 is rotated to move the entire cell. In FIG. 7, 16c moves in the direction of 16b. Timing 3: In order to perform fluorescence spectrometry on the cell 16f, the fluorescence spectrometry high-intensity light source 13 is caused to emit light.
The light source 24 for absorbance spectrometry is stopped from emitting light (turned off), and fluorescence spectrometry is performed. Timing 4: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

FIG. 11 is a diagram showing a procedure of only the absorbance spectrometry using the embodiment 2 of FIG. 7 or 8 of the present invention. Timing 1: In order to perform absorbance spectrometry on the cell 16b, the light source 24 for absorbance spectrometry is caused to emit light, and the high-intensity light source 13 for fluorescence spectrometry is stopped from emitting light (turned off).
Perform absorbance spectroscopy. Timing 2: The reaction tank 6 is rotated to move the entire cell. In FIG. 8, 16c moves in the direction of 16b. Timing 3: In order to perform absorbance spectroscopy measurement on the cell 16c, the cell is left in the previous state (the light source 24 for absorbance spectroscopy is made to emit light, but the high-intensity light source 13 for fluorescence spectroscopy is stopped), and the absorbance spectroscopy is performed Execute. Timing 4: The reaction tank 6 is rotated to move the entire cell. Hereinafter, this is repeated.

According to the second embodiment of FIG. 7 or 8 of the present invention, the absorbance spectrometry and the fluorescence spectrometry can be performed by one automatic analyzer, and the conventional absorbance spectrometer and the fluorescence spectrometer can be used. As compared with the case where each is installed, the amount of inspection work can be reduced, the installation scale of the device can be reduced, the amount of blood collected from the patient can be reduced, the burden on the patient can be reduced,
Equipment costs can be reduced.

According to the second embodiment of FIG. 7 or FIG. 8 of the present invention, in addition to the features of the first embodiment of FIG. 2 or FIG. , High frequency measurement. In addition, since the condenser mirror 21 is not inserted into the cell, when the concentration of the component to be measured in the sample to be measured is low and a lot of noise is added to the measurement signal in the absorbance spectrometry, the measurement optical system is not used. Switch to fluorescence spectrometry for accurate measurement.

The first embodiment of the present invention shown in FIG.
According to the embodiment 2 of FIG. 7 or FIG. 8, when a reagent for absorbance spectrometry is prepared for a component which can be conventionally measured only by fluorescence spectrometry, the fluorescence spectrometry is changed to absorbance spectrometry. Can be dealt with by switching the procedure from FIG. 5 to FIG. 6 or from FIG. 10 to FIG.

[0029]

According to the present invention, the absorbance spectrometry and the fluorescence spectrometry can be performed by one automatic analyzer, and the conventional absorbance spectrometer and the fluorescence spectrometer can be installed separately. In comparison, the amount of inspection work can be reduced, the scale of installation of the device can be reduced, the amount of blood collected from the patient can be reduced, the burden on the patient can be reduced, and the cost of the device can be reduced.

In the fluorescence spectrometry, when the concentration of the component to be measured in the sample to be measured is high and the quenching of the concentration of the fluorescence is foreseen, the measurement is switched from the fluorescence spectrometry to the absorbance spectrometry. Measurement. On the other hand, in the absorbance spectroscopy, when the concentration of the component to be measured in the sample to be measured is low and a lot of noise is added to the measurement signal,
By switching the measurement optical system to fluorescence spectrometry, accurate measurement can be performed.

[Brief description of the drawings]

FIG. 1 is a diagram schematically showing the appearance of an embodiment of an automatic analyzer according to the present invention.

FIG. 2 is a perspective view showing a system configuration structure when the spectrophotometer according to the first embodiment of the present invention is used for fluorescence spectrometry.

FIG. 3 is a perspective view showing a system configuration when the optical system of the spectrophotometer of FIG. 2 is used for absorbance spectrometry.

FIG. 4 is a diagram showing the procedure of fluorescence spectrometry and absorbance spectrometry using the embodiment of FIG. 2 or 3 of the present invention.

FIG. 5 is a diagram showing a procedure for only fluorescence spectrometry using the embodiment of FIG. 2 or 3 of the present invention.

FIG. 6 is a view showing a procedure of only the absorbance spectrometry using the embodiment of FIG. 2 or 3 of the present invention.

FIG. 7 is a perspective view showing a system configuration when the second embodiment of the spectrophotometer according to the present invention is used for fluorescence spectrometry.

8 is a perspective view showing a system configuration when the optical system of the spectrophotometer of FIG. 7 is used for absorbance spectroscopy.

FIG. 9 is a diagram showing a procedure of fluorescence spectrometry and absorbance spectrometry using the embodiment of FIG. 7 or 8 of the present invention.

FIG. 10 is a diagram showing a procedure of only fluorescence spectrometry using the embodiment of FIG. 7 or 8 of the present invention.

FIG. 11 is a diagram showing a procedure of only absorbance spectrometry using the embodiment of FIG. 7 or 8 of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Automatic analyzer 2 Operation surface of automatic analyzer 3 Sampler 4 Sample pipetter 5 Spectrophotometer 6 Reaction tank 7 Washing mechanism 8 Stirring mechanism 9 Reagent pipetter 10 Reagent bottle 11 Reagent tank 1 12 Reagent tank 2 13 High-intensity light source 14 Condenser Lens 15 Filter 16 a to 16 f Cell 17 a to 17 e Transmission window 18 Sample to be measured 19 Beam stopper 20 Slit for absorbance spectrometry 21 Condenser mirror 22 Spectral element 23 Photodetector array 23 a to 23 b Photodetector 24 Light source for absorbance spectrometry 25 Shutter 26 Condenser Lens 30b, 30c, 30f Condensing mirror 31 Fluorescence spectroscopic measurement slit 32 Photodetector array 32a Photodetector

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Hiroshi Maki 882 Ma, Hitachinaka-shi, Ibaraki Pref.

Claims (5)

[Claims] 1. A spectrophotometer for an automatic analyzer for automatically analyzing chemical components contained in human blood, urine, etc., comprising: a reaction vessel including a plurality of cells; a high-intensity light source for fluorescence spectrometry; An optical system for selecting light of a predetermined wavelength from the light source and condensing the light on the cell, and a condensing mirror disposed in a cell adjacent to the cell and condensing fluorescent light of the sample to be measured excited in the cell And an absorbance spectrometry slit for passing the collected fluorescence light,
A spectroscopic element that disperses fluorescence transmitted through the slit for absorbance spectrometry, a photodetector array that selectively detects light having a target wavelength that is disperse and measures fluorescence spectroscopy, a light source for absorbance spectrometry, and the absorbance An optical system for selecting light of a predetermined wavelength from a light source for spectrometry, transmitting the light to a sample to be measured, and condensing the light on the slit for spectroscopic measurement, and selectively detecting light of a target wavelength separated by the spectroscopic element A photodetector array for measuring absorbance spectroscopy, and switching means for selectively irradiating the cell with light from the high-intensity light source for fluorescence spectrometry and light from the light source for absorbance spectrometry. Features a spectrophotometer. 2. The spectrophotometer according to claim 1, wherein said condenser mirror has a mechanism for inserting / extracting said adjacent cell. 3. The spectrophotometer according to claim 1, wherein said converging mirror is fixedly provided in every other cell of said reaction tank. 4. A spectrophotometer for an automatic analyzer for automatically analyzing chemical components contained in human blood, urine, etc., comprising: a reaction vessel including a plurality of cells; a high-intensity light source for fluorescence spectrometry; An optical system for selecting light of a predetermined wavelength from the light source and condensing the light on the cell, and a condensing mirror fixedly disposed at a position adjacent to the cell and condensing fluorescent light of the sample to be measured excited in the cell And a slit for absorbance spectrometry for passing the collected fluorescence light, a spectroscopic element for dispersing the fluorescence transmitted through the slit for absorbance spectrometry, and selectively detecting the separated light of the target wavelength. A photodetector array for measuring fluorescence spectroscopy, a light source for measuring absorbance spectroscopy, and light having a predetermined wavelength selected from the light source for measuring absorbance spectroscopy, transmitting the light to the sample to be measured, and condensing the light on the slit for measuring spectroscopy. An optical system, a photodetector array for selectively detecting light of a target wavelength separated by the spectroscopic element and measuring absorbance spectroscopy, and light from the fluorescence spectrometry high-intensity light source and light from the absorbance spectrometry light source. Switching means for selectively irradiating the cell with light. 5. A sampler for holding a sample such as human blood or urine, a sample pipettor for collecting a sample, a spectrophotometer, a reaction tank, a washing mechanism, a stirring mechanism, a reagent pipettor, and a reagent bottle. And an automatic analyzer for analyzing a chemical component contained in the sample, comprising a plurality of reagent tanks, wherein the spectrophotometer according to any one of claims 1 to 4 is used as the spectrophotometer. An automatic analyzer characterized by comprising: