JPH11226304A - Apparatus and method for refining or reaction - Google Patents

Abstract

PROBLEM TO BE SOLVED: To use even a material excepting silica gel by preventing treating liquid from flowing through a surface layer of a packed layer by method wherein a doughnut state packed layer sandwiched between a disc and a doughnut state cover is rotated by centering its central axis. SOLUTION: When a treating liquid is supplied from a treating liquid supply nozzle 11 while a disc 4 is rotated via a rotary shaft 8 with a rotary mechanism, the treating liquid firstly forms a retention layer 13 inside a packed layer 5 sandwiched between the disc 4 and a cover 6, and then moves in an outward direction in the packed layer 5. The treating liquid which passed through the packed layer 5 is shaken out from an outer periphery, gathered in a groove 3, moves to the lowest end, and is discharged from a wastewater opening. Therefore, the packed layer 5 is not limited to silica gel, various kinds can be selected, the packed layer 5 does not fly about from the surface of the packed layer, and the treating liquid can move surely in the packed layer without flowing by gliding on the surface layer of the packed layer. Further, a treating liquid supply rate is so adjusted that the retention layer 13 is formed in a specific shape, and an appropriate treating liquid supply rate can be obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a purification or reaction apparatus and a purification or reaction method, and more particularly to a purification or reaction apparatus and a purification or reaction method applied to chromatography or the like.

[0002]

2. Description of the Related Art Diatomaceous earth is filled as a filter aid so as to be in contact with the outer wall of a cylindrical porous body, and the porous body is rotated about its central axis to filter from the outside to the inside. The device is commonly used.

In chromatography, a thin layer of silica gel stationary phase is formed in a concentric donut shape on a glass disk.
A centrifugal chromatographic apparatus has been put to practical use in which a sample is supplied to the inner periphery of the silica gel while being fixed and rotating the disk, and then developed and separated with a solvent while being moved in the outer peripheral direction by centrifugal force.

In these filtration devices and centrifugal chromatographs, when forming a packed bed of a filter aid or silica gel,
It is difficult to fill the filler with great gravity. In the filling of the filter aid, the filter aid suspension is often poured from the outside to the inside of the porous body to trap the filler on the outer wall surface of the porous body to form a packed layer. As the thickness becomes thicker, clogging occurs and the flow rate of the suspension decreases, so that the thickness of the packed bed naturally has a limit. Further, since the filling power is weak, when the surface is scraped, the filling layer is broken.

In the centrifugal chromatographic apparatus, when spinning, a force of centrifugal force is applied to the silica gel itself so that the silica gel itself jumps out in the outer peripheral direction. However, the adhesive force between the silica gels and the glass fixing the silica gel causes the outer peripheral direction to be reduced. To prevent it from jumping out. However, since the adhesive force is not large, the centrifugal force applied to the silica gel cannot be increased, and thus the thickness of the silica gel is limited.

Further, since gels need to have an adhesive strength enough to withstand centrifugal force, usable gels are limited, and silica gel is currently commercially available.

Further, in the method for producing a gel, a gel suspension is placed on a disk and then dried, so that not only the density cannot be increased but also the adhesive force between the gels is weak. And for these reasons, a large amount of processing could not be performed with a centrifugal chromatograph.

When a sample is supplied to the center of silica gel and developed with a solvent using this centrifugal chromatograph, the moving speed of the solvent is higher in the surface layer of silica gel than in the vicinity of the glass surface on which the silica gel is fixed. The development was faster than near the glass surface, and the separation and purification performance was poor. In addition,
When the silica gel was made as thick as possible and the thickness was 6 mm or less, the purification performance was worse as the thickness was larger.

Further, in the centrifugal chromatograph, the centrifugal force varies depending on the radius of rotation of the packed bed, and the flow rate of the processing liquid is larger at the outer circumference, so that the supply of the processing liquid from the inner circumference becomes insufficient and air is sucked from the surface layer. In order to avoid this, if an excessive treatment liquid is supplied to the inner periphery, there is a disadvantage that the surface of the packed bed slides and moves to the outer periphery.

Further, when the processing liquid is supplied to the inner periphery of the packed bed, if the treatment liquid is supplied excessively as described above, the surface slides and moves to the outer periphery, and if it is insufficient, gas is sucked. At the present time, there is no index for selecting.

On the other hand, fixed-bed reactors are used in various fields. In this method, a reaction solution is allowed to flow through a fixed bed to cause a reaction. However, the reaction solution easily flows only in a portion of the fixed bed having a small pressure loss, and it is difficult to uniformly and effectively use the entire fixed bed. . This is because no force for flowing the reaction solution is applied to a place where the pressure loss is large, in other words, no force in the flow direction is applied to all of the reaction solutions in the fixed bed.

The present invention has been made in view of the above-mentioned problems of the conventional purification or reaction apparatus, and it is intended to prevent a treatment liquid from flowing through a surface layer of a packed bed, and to be able to use materials other than silica gel. Enables the filling process to be performed by applying centrifugal force to the packing material, applying a force in the flow direction to all the processing liquid flowing in the packed bed, and increasing the height of the packed bed. It is an object of the present invention to provide a purification or reaction device and a purification or reaction method.

[0013]

Means for Solving the Problems The inventor of the present invention has conducted intensive studies in order to achieve the above object. As a result, if a cover is provided in close contact with the surface layer of the packed layer, the processing solution (including the reaction solution) can be treated with the surface layer. To prevent gas from flowing in or sucking gas from the surface layer. If the cover forms an eave that protrudes inside the packed layer, the processing liquid accumulates in the portion sandwiched between the eave and the bottom, so if the processing liquid is supplied at a flow rate where a certain amount of the processing liquid is held It has been found that an appropriate processing liquid supply rate can be obtained, a packed bed can be formed by centrifugation, and the above object can be achieved by supplying a processing liquid to such a packed bed with centrifugal force. I came to.

[0014] The invention according to claim 1 is a purification or reaction apparatus, comprising a housing, and disposed in the housing,
It is characterized by comprising a disk, a donut-shaped filling layer sandwiched between donut-shaped covers, and means for rotating the filling layer about its central axis.

According to a second aspect of the present invention, the filling layer further comprises a cylindrical porous body arranged concentrically, and the cylindrical porous body comprises an outer periphery of the filling layer, the disk, and the donut shape. The donut-shaped cover is in contact with the cover, and has an eave portion projecting inward from the filling layer.

According to a third aspect of the present invention, a flow rate limiting layer is provided on the outer peripheral portion of the packed layer.

According to a fourth aspect of the present invention, the height of the filling layer is 50 mm or more.

According to a fifth aspect of the present invention, there is provided a cylindrical porous body having a housing, an eaves portion disposed inside the housing and having a bottom portion and an inwardly projecting portion, and the cylindrical porous body having a cylindrical center. A packed bed forming apparatus provided with means for rotating about a shaft.

The invention according to claim 6 is characterized in that the packed bed is a chromatogel stationary phase, and a means for supplying a sample and a developing solvent to the stationary phase is provided.

According to a seventh aspect of the present invention, the inner wall of the filling layer is arranged while rotating the disk and the donut-shaped filling layer sandwiched between the donut-shaped covers around the central axis thereof. The process liquid is supplied to the apparatus.

The invention according to claim 8 is a purification or reaction method, wherein a cylindrical porous body is concentrically formed with the packing layer so as to be in contact with the outer periphery of the packing layer, the disk, and the donut-shaped cover. The donut-shaped cover is provided with an eave portion that extends inward from the filling layer, and the processing liquid is supplied to the inner wall of the filling layer while rotating the cylindrical porous body together with the filling layer. I do.

According to a ninth aspect of the present invention, the packed layer is a chromatogel stationary phase, and a sample is supplied to the inner wall, and then a developing solvent is continuously supplied to purify the sample.

According to a tenth aspect of the present invention, a cylindrical porous body having a bottom portion and an eaves projecting inward is disposed in a housing, and the cylindrical porous body is rotated about a central axis of the cylindrical porous body. The method is characterized in that a filler suspension is supplied to the inner wall of the porous body to form a packed layer.

According to the present invention, the packing layer is not limited to silica gel, but can be variously selected. The packing layer does not scatter from the surface of the packing layer, and the treatment liquid can flow on the surface layer of the packing layer. It is possible to move the inside of the packed bed without fail, without sucking gas from the surface layer into the packed bed.When the processing liquid is supplied, a holding layer is formed on the inner wall of the packed bed, and this holding layer has a predetermined shape. By adjusting the processing liquid supply rate so as to form the processing liquid, an appropriate processing liquid supply rate can be obtained, and the above-described conventional disadvantage can be solved.

Further, according to the present invention, the rotation of the cylindrical porous body applies a force in the outer peripheral direction to all the liquids in the packed bed, so that the liquid flows even in a place where the pressure loss is large. Also,
Since the processing liquid can be moved with a larger gravity than before, the processing speed can be increased and the amount of the processing liquid can be reduced. Further, after the purification or the like, the cleaning liquid can be moved in the outer peripheral direction in the packed layer for cleaning, so that the filler can be effectively reused.

Furthermore, according to the present invention, the higher the height of the packed bed, the larger the amount of processing, and the ratio of the upper end to the lower end with respect to the entire height, which causes a difference in the moving speed of the processing liquid, becomes smaller. , Uniform purification and reaction are possible. By setting the height of the silica gel packed layer to 50 mm or more, a recovery rate of about 90% or more can be obtained in the case where cinnamate alcohol is purified by using silica gel.

Further, according to the present invention, since the filler can be filled while applying a centrifugal force, the packed layer can be uniformly and compacted. That is, for example, it is easy to add 100 G to the filler or the processing liquid by rotation.

[0028]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Next, specific examples of embodiments of the purification or reaction apparatus according to the present invention will be described with reference to the drawings.

FIG. 1 is a schematic sectional view showing a first embodiment of the purification or reaction apparatus according to the present invention. The device 1 includes a housing 2, a disk 4, a donut-shaped filling layer 5, a donut-shaped cover 6, a rotating shaft 8, and the like.

The housing 2 is formed in a hollow cylindrical shape having a low height, in which a disk 4, a filling layer 5 provided on the upper surface thereof, and a cover 6 are laminated, and a plurality of fixed members are provided. These are integrally configured by the tool 7. The housing 2 is disposed so as to be obliquely inclined. A groove 3 is formed in the inner wall of the housing 2, and a drain port 12 is provided at the lowermost end of the groove 3.

The housing 2 is provided with a removable lid 10, and a processing liquid supply nozzle 11 is provided through the lid 10.

On the upper surface of the filling layer 5, a cover 6 which is in contact with the filling layer 5 and protrudes in the inner circumferential direction thereof;
The disk 4 provided on the bottom surface of the filling layer 5 is provided to hold the processing liquid on the inner periphery of the filling layer 5.

That is, when the rotation of the packed bed 5 is stopped,
A disk 4 is provided so that the treatment liquid does not fall,
When the rotation of is increased, a force for moving the processing liquid upward is applied, so the cover 6 is provided.

Here, the packing layer 5 does not have the function of being fixed to each other as in the case of conventional silica gel,
And the cover 6 is pressed down, so that a generally used filler such as an ion exchange resin and cellulose can be used. The material of the cover 6 and the lid 10 is not particularly limited as long as it is not affected by the processing solution to be used. However, if the material is made of a transparent material, the inside can be observed and fluorescence can be observed by irradiating ultraviolet rays. The material of the disk 4 can be selected from metals, ceramics, plastics, and the like as well as glass.

The thickness of the filling layer 5, that is, the moving distance of the processing liquid may be selected depending on the purpose, but the higher the height, the larger the processing amount. When the present apparatus 1 is applied to chromatography or chemical reaction, it is desirable that the liquid flows at a uniform flow rate in the entire height direction, but the moving speed of the processing liquid at the upper end, the center, and the lower end of the packed bed 5. Are different. If the height of the packed layer 5 is increased, the ratio of the upper end portion to the lower end portion is reduced, so that uniform purification and reaction can be performed.

The rotating shaft 8 is fixed integrally with the disk 4. The rotating shaft 8 is provided with a bearing 9 fixed to the housing 2.
Through the housing and connected to a rotating mechanism (not shown).

When the above-mentioned purification or reaction apparatus is used, the processing liquid is supplied to the inner wall of the packed bed 5, and as the method, intermittent supply or continuous supply is selected depending on the application. In the case of continuous supply, it is common to use a pump, but it may be supplied by pressure or by using hydrostatic pressure. Even when the processing liquid is supplied to the bottom of the packed bed 5, the processing liquid forms a cylinder along the inner wall of the packed bed 5. The supply rate is efficient if treated water is always present on the inner wall of the packed bed 5. Further, it is also possible to supply the whole amount to be processed at once into the packed bed 5 and perform batch processing. When supplying gas to the filling layer 5, the inner wall of the filling layer 39 may be exposed to gas.

In the case of purification, a sample in which a plurality of components are mixed or a developing solvent, or both of them are used as the treatment liquid, and are used for separation / fractionation and purification of the components. In the case of the reaction, a reaction solution for performing a chemical reaction, an ion exchange reaction, a biochemical reaction, an adsorption reaction, or the like is used.

When the packed bed 5 is rotated about the central axis of the cylinder, a centrifugal force acts on the packed bed 5 and the liquid contained therein in the outer peripheral direction. Then, the processing liquid discharged to the outside after passing through the filling layer 5 is collected in the groove 3 and discharged from the drain port 12.

When the processing liquid is supplied from the processing liquid supply nozzle 11 while rotating the disk 4 via the rotation shaft 8 by a rotating mechanism (not shown), the processing liquid firstly fills the filling layer 5 sandwiched between the disk 4 and the cover 6. The holding layer 13 is formed on the inside, and then the inside of the filling layer 5 is moved outward. The processing liquid that has passed through the packed bed 5 is spouted from the outer periphery, gathers in the groove 3, moves to the lowermost end, and is discharged from the drain port 12.

According to the above embodiment, the packing layer is not limited to silica gel, but can be variously selected. The packing layer does not scatter from the surface of the packing layer. The holding layer 13 is formed when the processing solution is supplied without moving the inside of the packing layer without fail, preventing the gas from being sucked from the surface layer into the filling layer.
By adjusting the processing liquid supply rate so that the liquid is formed into a predetermined shape, there is an advantage that an appropriate processing liquid supply rate can be obtained, and the conventional disadvantage can be solved.

Further, as shown in FIG. 1, by extending the cover 6 and the disk 4 outside the filling layer 5, the processing liquid spouted from the filling layer 5 can be reliably contained in the groove 3. . When a light-transmitting flexible material is used as the cover 6, a cover such as a metal net through which light can pass may be disposed and the cover 6 fixed.

Furthermore, if the pressure loss of the packed bed is small, the processing liquid is too fast for purification or reaction, or the processing liquid moves with a small centrifugal force. Force for moving the processing solution may be insufficient. In order to avoid this, it is preferable to provide an unillustrated flow rate limiting layer on the outer periphery of the packed layer.

The flow rate limiting layer can be made of a material such as a net which has a slightly low water permeability. The material used may be a material which does not react with a processing liquid such as plastic, alumina, silica or the like. It is not necessary to have a reaction function. It is preferable that the flow rate limiting layer reduces the flow rate of the processing liquid to half or less.

FIG. 2 is a schematic sectional view showing a second embodiment of the purification or reaction apparatus according to the present invention. This device 31 includes a housing 32, a cylindrical porous body 33, a filling layer 39,
It is constituted by a rotating shaft 36 and the like.

The housing 32 has a cylindrical porous body 3 inside.
It is formed in a hollow cylindrical shape having a space for accommodating 3 and the like, and a drain port 41 is provided at the bottom thereof.

The cylindrical porous body 33 has a bottom portion 34 and a donut-shaped eave portion 35 and is housed inside the housing 32. At the center of the cylindrical porous body 33, a rotating shaft 36 is fixed integrally with the bottom 34. The rotating shaft 36 is connected to a rotating mechanism (not shown) via a bearing 37 fixed to the housing 32. Further, inside the cylindrical porous body 33, a filter cloth 38 having a hole smaller than the hole is closely arranged.

As will be described later, the cylindrical porous body 33 needs to withstand the centrifugal force generated when it rotates and allow the supplied processing liquid to pass outside, and is made of metal such as stainless steel or titanium. It is formed of a synthetic resin net or a perforated plate.

At the upper end of the cylindrical porous body 33, an eaves portion 35 which is in contact with the cylindrical porous body 33 and protrudes in the inner circumferential direction, and a bottom portion 34 provided at the lower end of the cylindrical porous body 33 are treated. It is provided to hold the liquid on the inner periphery of the filling layer 39.

That is, when the rotation of the cylindrical porous body 33 is stopped, a bottom portion 34 is provided so that the processing liquid does not drop, and when the rotation of the cylindrical porous body 33 is increased, the processing liquid is moved upward. An eave portion 35 is provided to apply a force to cause the eaves to be applied.

The pores formed in the cylindrical porous body 33 are further provided with another porous body having a smaller diameter than the above-mentioned particles in contact with the inner periphery of the cylindrical porous body 33 even if the pores are larger than the filler particles. In addition, even if the particles are smaller than the holes, the particles need not pass through the holes due to agglomeration or clogging.

Various materials and materials are selected for the filling material used for the filling layer 39 depending on the purpose. cellulose,
In addition to alumina, metal particles, silica gel, zeolite, glass particles, plastic particles and fibers, etc., urethane and cellulose having continuous cells, and further subjected to porosity treatment, ion exchange treatment, addition of reactivity, microorganisms and enzymes An example is a material in which a physiologically active substance such as antibody or antibody is immobilized or a combination thereof is used. In addition, the combined material is a material manufactured by mixing the above materials, filling a plurality of materials in multiple layers, changing the particle size of the same material into multiple layers, and a combination of these materials. Applicable.

When the above-mentioned filling layer 39 is formed, the cylindrical porous body 33 is rotated about its central axis, and a filler suspension is supplied to its inner wall. The filler in the suspension is tightly packed in the porous body 33 by filtration or sedimentation centrifugal force, or both. The structure of the apparatus is simplified when the rotating means for forming the packed bed 39 is shared with the rotating means for the packed bed 39 during purification or reaction. The method of supplying the suspension may be batch supply or continuous supply, but is selected depending on the purpose. The thickness of the filling layer 39 can be selected in the same manner as in the first embodiment.

In a conventional centrifugal chromatograph, 6 mm
Purification of cinnamate alcohol was performed using thick silica gel, and the distribution of cinnamate alcohol in the height direction from the glass surface on which the silica gel was fixed was examined while shaving the silica gel. Since the distribution of about 3 mm was large, it was found that the height of 5 mm above and below 6 mm should be increased to a negligible height. 9
Assuming a recovery rate of 0%, the thickness should be 50 mm or more.

When the cylindrical porous body 33 and the filling layer 39 in contact with the inner wall thereof are rotated about the cylindrical central axis, the liquid contained in the filling layer 39 and the inside thereof, and the liquid in contact with the surface, have a cylindrical shape. Centrifugal force acts on the outer periphery of the porous body 33. The processing liquid discharged to the outside of the cylindrical porous body 33 is collected in the housing 32 and discharged from the drain port 41.

As described above, the disk 4 is rotated with the packed layer 5 disposed above the disk 4. The mechanism is a general technique in a basket type centrifuge. The purification or reaction does not require as much centrifugal force as a centrifuge,
The basic rotation mechanism may be the same as that of the centrifuge.

When the cylindrical porous body 33 rotates, the packed layer 3
9 forms a cylinder along the inner wall of the packed bed 39 and moves into the packed bed 39 due to centrifugal force. At this time, if the rotation speed is low, the gravity of the processing solution causes a downward movement. Therefore, it is preferable to apply a centrifugal force of 5 G or more on the inner wall of the packed layer 39.

Next, an embodiment in which the apparatus 31 is applied to chromatography will be described below.

When the silica gel suspension is supplied into the cylindrical porous body 33 while rotating the cylindrical porous body 33,
Silica gel is tightly packed in the filter cloth 38. This is dried to form a filling layer 39. This packed layer 39 becomes a stationary phase.

Next, the operation when this apparatus 31 is used for purification will be described. First, when the developing solvent is supplied while rotating the stationary phase by rotating the rotating shaft 36, the developing solvent moves in the outer peripheral direction in the stationary phase by centrifugal force. When the sample to be purified is supplied to the inner wall of the stationary phase after the interior of the stationary phase is filled, the sample forms a cylinder 40 and is absorbed into the stationary phase. Therefore, when the developing solvent is continuously supplied again at a speed at which the inner wall of the stationary phase does not come into contact with the gas, the sample is separated and purified while moving in the stationary phase. Since the developing solvent containing the purified component is discharged from the drain port 41, it may be separated for each component.

When this apparatus 31 is used for purification, the processing liquid near the eaves 35 and the bottom 34, ie, the sample or the developing solvent, or the moving speed of both of them is different at the upper end and the lower end from the central part. As a cause, there is often no substantial problem if the height of the stationary phase is 50 mm or more. Although an appropriate centrifugal force is often different between the step of forming the packed layer 39 and the purification step, it can be dealt with by appropriately selecting the rotation speed.

Further, the stationary phase subjected to purification is often reusable by washing. That is, in the case of the silica gel stationary phase, after the purification, the washing liquid such as methanol may be moved in the stationary phase in the outer peripheral direction by the same method to be washed. Due to the rotation, a force in the outer peripheral direction is applied to all the liquids in the filling layer 39, so that the liquid flows even at a large pressure loss. Since the processing solution can be moved with a larger centrifugal force than a conventional chromatographic apparatus, the processing speed is high and the amount of developing solvent can be reduced. In addition, in the method of forming the filling layer 39, since the filling can be performed while applying the centrifugal force, the filling can be uniformly and compacted.

The device 31 can be used for various reactions. For example, a carrier on which microorganisms or enzymes are immobilized can be subjected to a biochemical reaction as the packed layer 39. Alternatively, a porous carrier can be filled, and the carrier can be attached as a cell scaffold and used for culture.

[0064]

According to the present invention, various materials can be selected as the material of the filling layer, the filling layer does not scatter from the surface of the filling layer, and the processing liquid can move in the filling layer without fail. Without inhaling gas from the surface layer into the packed bed,
It is possible to provide a purification or reaction device and a purification or reaction method capable of obtaining an appropriate processing solution supply rate.

According to the present invention, it is possible to provide a purification or reaction apparatus and a purification or reaction method that can perform purification and reaction in a short time, require a small amount of processing solution, and can effectively use a filler.

Further, according to the present invention, uniform purification and reaction become possible. And, the height of the silica gel packed bed is 50
When the diameter is at least mm, it is possible to provide a purification or reaction apparatus and a purification or reaction method capable of obtaining a recovery rate of about 90% or more when, for example, cinnamate alcohol is purified.

Further, according to the present invention, it is possible to provide a purification or reaction apparatus and a purification or reaction method capable of uniformly and compacting a packed bed.

[Brief description of the drawings]

FIG. 1 is a schematic sectional view showing a first embodiment of a purification or reaction apparatus according to the present invention.

FIG. 2 is a schematic sectional view showing a second embodiment of the purification or reaction apparatus according to the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Purification or reaction apparatus 2 Housing 3 Groove 4 Disk 5 Filling layer 6 Cover 7 Fixing tool 8 Rotating shaft 9 Bearing 10 Cover 11 Treatment liquid supply nozzle 12 Drainage port 13 Retention layer 31 Purification or reaction apparatus 32 Housing 33 Cylindrical porous body 34 Bottom 35 Eaves 36 Rotating shaft 37 Bearing 38 Filter cloth 39 Packing layer 40 Cylinder (sample) 41 Drain outlet

Claims (10)

[Claims] 1. A housing comprising: a housing, a disk, a donut-shaped filling layer sandwiched between donut-shaped covers, and means for rotating the filling layer about its central axis. A purification or reaction apparatus characterized by the above-mentioned. 2. The filling layer further comprises a cylindrical porous body disposed on a concentric circle, wherein the cylindrical porous body contacts an outer periphery of the filling layer, the disk, and the donut-shaped cover. The refining or reaction apparatus according to claim 1, wherein the cover has an eave portion projecting inward from the packed bed. 3. The purification or reaction apparatus according to claim 1, wherein a flow rate limiting layer is provided on an outer peripheral portion of the packed bed. 4. The purification or reaction apparatus according to claim 1, wherein the height of the packed bed is 50 mm or more. 5. A housing, a cylindrical porous body disposed inside the housing and having a bottom and an eaves projecting inward, and rotating the cylindrical porous body about a central axis of the cylindrical porous body. The purification or reaction apparatus according to any one of claims 1 to 4, further comprising a packed bed forming apparatus having a means for causing the packed layer to be formed. 6. The method according to claim 1, wherein the packed bed is a chromatogel stationary phase, and a means for supplying a sample and a developing solvent to the stationary phase is provided. Purification equipment. 7. A treatment liquid is supplied to the inner wall of the filling layer while rotating the disk and the donut-shaped filling layer sandwiched between the disk and the donut-shaped cover around the central axis thereof. A purification or reaction method, comprising: 8. A cylindrical porous body is disposed concentrically with the filling layer so as to be in contact with the outer periphery of the filling layer, the disk, and the donut-shaped cover, and the donut-shaped cover has an inner side with respect to the filling layer. The purification or reaction method according to claim 7, wherein a protruding eave portion is provided, and the processing liquid is supplied to the inner wall of the packed layer while rotating the cylindrical porous body together with the packed layer. 9. The method according to claim 7, wherein the packed bed is a chromatogel stationary phase, and the sample is supplied to the inner wall, and then the developing solvent is continuously supplied to purify the sample. Purification method. 10. A cylindrical porous body disposed in a housing and having a bottom portion and an eaves projecting inward is rotated about a central axis of the cylindrical porous body, and is rotated on an inner wall of the cylindrical porous body. The purification or reaction method according to any one of claims 7 to 9, wherein a suspension of the filler is supplied to form a packed bed.