JPH1059911A - Crystallization of acidic amino acid - Google Patents

Crystallization of acidic amino acid

Info

Publication number
JPH1059911A
JPH1059911A JP21618696A JP21618696A JPH1059911A JP H1059911 A JPH1059911 A JP H1059911A JP 21618696 A JP21618696 A JP 21618696A JP 21618696 A JP21618696 A JP 21618696A JP H1059911 A JPH1059911 A JP H1059911A
Authority
JP
Japan
Prior art keywords
acidic amino
amino acid
crystals
crystallization
glutamic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP21618696A
Other languages
Japanese (ja)
Inventor
Yasushi Kawabata
Takeshi Nakagiri
武志 中桐
泰 川端
Original Assignee
Ajinomoto Co Inc
味の素株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc, 味の素株式会社 filed Critical Ajinomoto Co Inc
Priority to JP21618696A priority Critical patent/JPH1059911A/en
Publication of JPH1059911A publication Critical patent/JPH1059911A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C227/00Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C227/38Separation; Purification; Stabilisation; Use of additives
    • C07C227/40Separation; Purification
    • C07C227/42Crystallisation

Abstract

PROBLEM TO BE SOLVED: To provide a method for crystallizing an acidic amino acid, enabling to crystallize highly pure acidic amino acid crystals from an acidic amino acid- containing solution in a high yield (high acquisition rate). SOLUTION: When acidic amino acid crystals are deposited from an acidic amino acid-containing solution containing a certain coloring substance and impurities such as proteins, a nonionic surfactant is added, thereby enabling to deposit the large acidic amino acid crystals effectively reduced in the contamination of the coloring substance without lowering the yield of the acidic amino acid.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION
【0001】[0001]
【発明の属する技術分野】本発明は酸性アミノ酸含有液
から高純度の酸性アミノ酸結晶を晶析分離する方法に関
するものである。
The present invention relates to a method for crystallizing and separating high-purity acidic amino acid crystals from a liquid containing acidic amino acids.
【0002】[0002]
【従来の技術】酸性アミノ酸のひとつであるグルタミン
酸の含有液からグルタミン酸結晶を晶出させる手段とし
ては、グルタミン酸含有液を酸でグルタミン酸の等電点
pH3.2前後に調整して晶析する方法や、グルタミン
酸含有液を濃縮後、冷却することにより晶析する方法等
が知られている。いずれの場合も晶析及びその後の分離
操作において、グルタミン酸含有液中の不純物の一部が
結晶側に移行する。特に発酵原料として色素等の不純物
含量が多い悪質なCM(ケーンモラセス)等を使用した
グルタミン酸発酵液(CM系グルタミン酸発酵液)を晶
析する場合には、結晶側に移行する不純物の量が多くな
り、その後の精製工程での精製度を上げる手段をとらな
ければ、製品規格を満足出来なくなる可能性が出てく
る。
2. Description of the Related Art As means for crystallizing glutamic acid crystals from a solution containing glutamic acid, which is one of acidic amino acids, a method of adjusting the glutamic acid-containing solution to about the isoelectric point of glutamic acid with an acid to about 3.2 or more by crystallization is available. A method is known in which a glutamic acid-containing liquid is concentrated and then cooled to crystallize. In any case, in the crystallization and the subsequent separation operation, some of the impurities in the glutamic acid-containing liquid migrate to the crystal side. In particular, when crystallizing a glutamic acid fermentation solution (CM-based glutamate fermentation solution) using a vicious CM (Kanemolasses) having a high impurity content such as a pigment as a fermentation raw material, the amount of impurities transferred to the crystal side is large. Unless measures are taken to increase the degree of refining in the subsequent refining process, there is a possibility that the product specifications cannot be satisfied.
【0003】不純物が結晶側に移行するには大きく分け
て2つの原因がある。1つは結晶成長の過程で結晶表面
に不純物が吸着した状態で成長を続け、結晶内部に不純
物が取り込まれる為であり、2つ目には分離した結晶に
母液が付着している為である。後者の場合には、付着し
た母液を洗浄除去することにより結晶側に移行する不純
物の量を容易に低減することが可能であるが、前者のよ
うに結晶内部に取り込まれた不純物は洗浄のみでは低減
することが出来ず、晶析の段階で何らかの工夫が必要で
ある。
There are roughly two causes for the migration of impurities to the crystal side. One is that the crystal continues to grow with the impurities adsorbed on the crystal surface during the crystal growth process, and the impurities are taken into the crystal, and the second is that the mother liquor adheres to the separated crystal. . In the latter case, it is possible to easily reduce the amount of impurities migrating to the crystal side by washing and removing the adhered mother liquor. It cannot be reduced, and some contrivance is required at the crystallization stage.
【0004】CM系グルタミン酸発酵液中には色素物
質、タンパク質、糖類、塩類、有機酸アミノ酸等の多種
多様な不純物が存在する。中でもある種の色素物質及び
タンパク質は両性電解質的性質を持ち、発酵液中(pH
7.0付近)では陰イオン性物質として存在している
が、グルタミン酸を晶析する目的でpHを3.2付近ま
で下げると、電気的に中性の状態で存在する割合が多く
なり、疎水的性質を有するようになる。このような状態
ではグルタミン酸結晶表面の疎水的部分との親和性が強
くなり、晶析時に結晶内に取り込まれやすくなると考え
られている。
[0004] A variety of impurities such as pigment substances, proteins, saccharides, salts, and organic acid amino acids are present in the CM glutamic acid fermentation liquor. Among them, certain pigment substances and proteins have ampholyte properties and are contained in fermentation broth (pH
(At around 7.0) as an anionic substance, but when the pH is lowered to around 3.2 for the purpose of crystallizing glutamic acid, the proportion present in an electrically neutral state increases and the hydrophobicity increases. It has a characteristic. In such a state, it is considered that the affinity with the hydrophobic portion on the surface of the glutamic acid crystal becomes strong, and the glutamic acid crystal is easily taken into the crystal during crystallization.
【0005】現在、工業的には晶析条件(温度、pH
等)を適切に制御することにより、この結晶内に取り込
まれる不純物量を抑える工夫をしている。
At present, industrially, crystallization conditions (temperature, pH,
Etc.) are appropriately controlled to reduce the amount of impurities taken into the crystal.
【0006】しかしながら、グルタミン酸の晶析率(取
得率)を上げようとすると、晶析温度を低くし、pHは
グルタミン酸の等電点付近である3.2前後で行うこと
になり、結果的に不純物がグルタミン酸結晶中に取り込
まれやすい条件になっている。
However, in order to increase the crystallization rate (acquisition rate) of glutamic acid, the crystallization temperature is lowered, and the pH is set to about 3.2, which is near the isoelectric point of glutamic acid. The condition is such that impurities are easily taken into the glutamic acid crystal.
【0007】過去、グルタミン酸発酵液からグルタミン
酸結晶を晶析する際に、ある種のカチオン性、アニオン
性及び両性の界面活性剤をグルタミン酸発酵液重量に対
して0.2〜1.0%添加することにより、結晶粒径を
大型化出来、分離の際に結晶表面に付着する付着母液量
を抑えて結晶側に移行する不純物量を低減出来るとの報
告がある(特許公報特公昭44−30249、特許公報
特公昭37−14709)。しかし、この方法も効果的
ではあるが、グルタミン酸含有液がより多くの不純物を
含んでいる場合、不純物を効率的に淘汰するためのより
いっそう効果的な方法が必要である。
In the past, when crystallizing glutamic acid crystals from a glutamic acid fermentation liquor, certain cationic, anionic and amphoteric surfactants are added in an amount of 0.2 to 1.0% based on the weight of the glutamic acid fermentation liquor. Accordingly, it is reported that the crystal grain size can be increased, the amount of mother liquor attached to the crystal surface during separation can be suppressed, and the amount of impurities transferred to the crystal side can be reduced (Japanese Patent Publication No. 44-30249, Japanese Patent Publication No. 37-14709). However, while this method is also effective, if the glutamic acid-containing solution contains more impurities, a more effective method for efficiently eliminating impurities is needed.
【0008】また、フェニルアラニン、トリプトファン
等の疎水性アミノ酸の晶析において、界面活性剤を添加
することにより結晶の晶癖を改善し、付着母液量を抑え
ることが出来るとの報告がある(国際公開番号 WO9
0/09372)。しかし、この報告には、親水性であ
る酸性アミノ酸に関する晶析についての記載はない。
[0008] In addition, it has been reported that in the crystallization of hydrophobic amino acids such as phenylalanine and tryptophan, the addition of a surfactant can improve the crystal habit and suppress the amount of mother liquor attached (International publication). No. WO9
0/09372). However, this report does not describe crystallization of acidic amino acids that are hydrophilic.
【0009】[0009]
【発明が解決しようとする課題】酸性アミノ酸含有液か
ら酸性アミノ酸結晶を取得する際に、酸性アミノ酸の晶
析率(取得率)を下げることなく、結晶内への不純物取
り込み量の少ない高純度の酸性アミノ酸結晶を効率的に
晶析する方法を提供することを目的とする。
SUMMARY OF THE INVENTION When obtaining acidic amino acid crystals from a solution containing acidic amino acids, a high-purity, low-impurity impurity is incorporated into the crystals without reducing the crystallization rate (acquisition rate) of the acidic amino acids. An object of the present invention is to provide a method for efficiently crystallizing acidic amino acid crystals.
【0010】[0010]
【課題を解決するための手段】本発明者らは、上記目的
を達成するために検討を行い、酸性アミノ酸含有液中に
界面活性剤を添加して晶析を行う際に、界面活性剤とし
てノニオン性界面活性剤を使用すると、驚くべきことに
アニオン性、カチオン性または両性界面活性剤に比べ、
結晶内への不純物の取り込み量の少ない、高純度の酸性
アミノ酸結晶を高晶析率で取得できることを見いだし、
本発明を完成した。すなわち、本発明は、酸性アミノ酸
の等電点晶析方法において、酸性アミノ酸含有液にノニ
オン性界面活性剤を添加し、晶析することを特徴とする
酸性アミノ酸の晶析方法に関するものである。
Means for Solving the Problems The inventors of the present invention have studied to achieve the above object, and have found that when adding a surfactant to a solution containing an acidic amino acid for crystallization, the surfactant is used as a surfactant. Using nonionic surfactants, surprisingly, compared to anionic, cationic or amphoteric surfactants,
It has been found that high purity acidic amino acid crystals can be obtained at a high crystallization rate with a small amount of impurities incorporated into the crystals,
The present invention has been completed. That is, the present invention relates to a method for crystallizing an acidic amino acid, which comprises adding a nonionic surfactant to a solution containing an acidic amino acid in the isoelectric point crystallization method for an acidic amino acid, followed by crystallization.
【0011】[0011]
【発明の実施の形態】本発明で用いられる酸性アミノ酸
含有液としては、等電点晶析に適用可能なものであれ
ば、使用可能であるが、不純物としてある種の色素及び
タンパク質等を含有する酸性アミノ酸含有液に対して、
本発明は特に効果的である。具体的にはグルタミン酸発
酵液(特許公開公報特開昭62−91194、特開平0
3−49690)、アスパラギン酸発酵液(特許公開公
報特開昭59−45895)、等電点晶析後分離した母
液、タンパク質の酸加水分解液、粗製グルタミン酸また
は粗製アスパラギン酸結晶の溶解液等があげられるが、
特にグルタミン酸含有液に本発明は有効である。
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The acidic amino acid-containing liquid used in the present invention can be used as long as it can be applied to isoelectric crystallization, but it contains certain dyes and proteins as impurities. For acidic amino acid-containing liquids
The present invention is particularly effective. Specifically, a glutamic acid fermentation solution (Japanese Patent Laid-Open Publication No. 62-91194,
3-49690), aspartic acid fermentation solution (JP-A-59-45895), mother liquor separated after isoelectric crystallization, acid hydrolysis solution of protein, solution of crude glutamic acid or crude aspartic acid crystal, etc. Can be given,
The present invention is particularly effective for a glutamic acid-containing liquid.
【0012】酸性アミノ酸含有液に添加されるノニオン
性界面活性剤としては、ポリオキシエチレン系、ソルビ
タン系、ショ糖系、グリセロール系、ポリグリセリン系
等のノニオン性界面活性剤が本発明に適用可能である
が、特にソルビタン脂肪酸エステルタイプまたはショ糖
脂肪酸エステルタイプのノニオン性界面活性剤が好まし
い。具体的にはソルビタン脂肪酸エステル構造を有する
ノニオンLT−221、ST−221、OT−221、
LP−20R(以上日本油脂(株)製)や、ショ糖脂肪
酸エステル構造を有するリョートーシュガーエステルL
WA−1540(三菱化成食品(株)製)を挙げること
ができる。
As the nonionic surfactant to be added to the acidic amino acid-containing liquid, nonionic surfactants such as polyoxyethylene, sorbitan, sucrose, glycerol and polyglycerin are applicable to the present invention. However, nonionic surfactants of the sorbitan fatty acid ester type or sucrose fatty acid ester type are particularly preferred. Specifically, nonionics LT-221, ST-221, OT-221 having a sorbitan fatty acid ester structure,
LP-20R (manufactured by NOF Corporation) and Ryoto Sugar Ester L having a sucrose fatty acid ester structure
WA-1540 (manufactured by Mitsubishi Kasei Food Co., Ltd.).
【0013】ノニオン性界面活性剤の添加量は、酸性ア
ミノ酸含有液重量に対して100ppm〜5000pp
mであり、500ppm〜1000ppmが特に好まし
い。
The amount of the nonionic surfactant added is 100 ppm to 5000 pp with respect to the weight of the acidic amino acid-containing solution.
m, and particularly preferably 500 ppm to 1000 ppm.
【0014】本発明における等電点晶析方法は、酸性ア
ミノ酸の等電点付近のpHで晶析を行う方法である。例
えば、酸性アミノ酸の一つであるグルタミン酸の等電点
晶析では、グルタミン酸の等電点であるpH3.2付近
でグルタミン酸の晶析が行われるが、晶析のpHは、
3.2〜4.0が好ましい。一方、アスパラギン酸の等
電点晶析のpHは、2.7〜3.5が好ましい。また、
適用可能な晶析の方法としては、中性に調整された酸性
アミノ酸含有液に酸を添加しながらpHを調整して晶析
する方法、等電点付近のpHに調整された酸性アミノ酸
含有液を冷却して晶析する冷却晶析法、等電点付近のp
Hに調整された酸性アミノ酸含有液を濃縮することによ
り晶析を行う濃縮晶析方法等通常行われている方法をを
挙げることができるが、酸性アミノ酸結晶が晶析できる
方法であれば、あらゆる等電点晶析方法が本発明におい
て適用可能である。
The isoelectric point crystallization method in the present invention is a method of performing crystallization at a pH around the isoelectric point of an acidic amino acid. For example, in the isoelectric point crystallization of glutamic acid, which is one of the acidic amino acids, glutamic acid is crystallized near pH 3.2, which is the isoelectric point of glutamic acid.
3.2 to 4.0 are preferred. On the other hand, the pH for isoelectric crystallization of aspartic acid is preferably from 2.7 to 3.5. Also,
Applicable crystallization methods include a method of adjusting the pH while adding an acid to a neutrally adjusted acidic amino acid-containing liquid and crystallization, and a method of adjusting an acidic amino acid-containing liquid adjusted to a pH near the isoelectric point. Crystallization method for cooling and crystallizing, p near the isoelectric point
Conventional methods such as a concentrated crystallization method of performing crystallization by concentrating the acidic amino acid-containing solution adjusted to H can be used, but any method capable of crystallizing acidic amino acid crystals can be used. Isoelectric point crystallization methods are applicable in the present invention.
【0015】酸性アミノ酸の等電点晶析においてpHを
酸性に調整する際に、ある種の色素物質及びタンパク質
は疎水的性質を有し、酸性アミノ酸結晶表面の疎水的部
分との親和性が強まり、結晶内に取り込まれ易くなる。
このような状態の中に、ノニオン性界面活性剤が存在す
ると、疎水的性質を有する色素物質やタンパク質に作用
して、これらを溶液中に分散させたり、あるいは酸性ア
ミノ酸結晶表面に作用して結晶表面をより親水状態にし
たりして、ある種の色素物質及びタンパク質の吸着、結
晶内取り込みを抑制したものと考えられる。
When the pH is adjusted to acidic in the isoelectric crystallization of acidic amino acids, certain pigment substances and proteins have hydrophobic properties, and the affinity with the hydrophobic portion of the acidic amino acid crystal surface increases. , Are easily taken into the crystal.
In such a state, when a nonionic surfactant is present, it acts on a pigment substance or protein having a hydrophobic property and disperses them in a solution or acts on an acidic amino acid crystal surface to form a crystal. It is considered that the surface was made more hydrophilic to suppress the adsorption of certain pigment substances and proteins and the incorporation into crystals.
【0016】また、このような状態下で晶析された結晶
の成長速度は、ノニオン性界面活性剤を添加しない場合
よりも大きくなり、比較的大型の結晶取得が可能とな
り、その後の分離工程において結晶に付着する母液の量
を抑制することが出来る。
In addition, the growth rate of the crystals crystallized in such a state becomes higher than that in the case where no nonionic surfactant is added, so that a relatively large crystal can be obtained. The amount of mother liquor attached to the crystal can be suppressed.
【0017】添加したノニオン性界面活性剤はその系内
の不純物及び酸性アミノ酸との密接な相互作用により酸
性アミノ酸精製効果を向上させていると考えられので、
存在する不純物の組成が異なると最適なノニオン性界面
活性剤の種類及び添加量も変化する。従って、最適なノ
ニオン性界面活性剤は、実際に本発明が適用される酸性
アミノ酸含有液を用いて評価して、選定すればよい。
Since the added nonionic surfactant is considered to enhance the effect of purifying the acidic amino acid by the close interaction with the impurities in the system and the acidic amino acid,
If the composition of the existing impurities differs, the type and the amount of the optimal nonionic surfactant also change. Therefore, the optimal nonionic surfactant may be selected by evaluating using an acidic amino acid-containing solution to which the present invention is actually applied.
【0018】[0018]
【実施例】以下、本発明を実施例により、具体的に説明
する。
The present invention will be specifically described below with reference to examples.
【0019】実施例1 特許公報特公平05−5477の実施例に記載の方法に
基づいて得られたグルタミン酸発酵液を35%塩酸でp
H3.2にして等電点晶析を行い、そのスラリーを50
℃にて約15時間撹拌後析出した結晶を分離し、分離母
液を得ることにより、50℃のグルタミン酸飽和溶液を
調製した。その溶液中に溶液中グルタミン酸重量に対し
て20%のグルタミン酸結晶(α晶)をシードし、2℃
/hrで10℃まで振とうしながら冷却晶析を行い、結
晶を分離した。分離した結晶は結晶重量の3倍量のグル
タミン酸飽和水溶液で3回リスラリー洗浄し、結晶に付
着した母液のほとんどを洗い流した洗浄結晶を得た。界
面活性剤を添加するものについては、冷却晶析を行う前
にグルタミン酸飽和溶液中に対飽和溶液重量で1000
ppm添加した。
Example 1 A glutamic acid fermentation liquor obtained according to the method described in the example of Japanese Patent Publication No. 05-5377 was prepared by adding 35% hydrochloric acid to p.
H3.2 and the isoelectric point crystallization was performed.
After stirring at about 15 ° C. for about 15 hours, the precipitated crystals were separated to obtain a separated mother liquor, whereby a glutamic acid saturated solution at 50 ° C. was prepared. Seed with 20% glutamic acid crystals (α crystals) based on the weight of glutamic acid in the solution in the solution,
Cooling crystallization was performed while shaking at 10 ° C./hr to separate crystals. The separated crystals were reslurried three times with a saturated aqueous solution of glutamic acid three times the weight of the crystals to obtain washed crystals in which most of the mother liquor attached to the crystals was washed away. For the case where a surfactant is added, before cooling and crystallizing, 1000 parts by weight in the glutamic acid saturated solution with respect to the saturated solution weight are added.
ppm was added.
【0020】添加した界面活性剤と得られた結晶性状に
ついて表1に示す。ショ糖脂肪酸エステルタイプのノニ
オン性界面活性剤であるリョートーシュガーエステルL
WAー1540の添加により、得られた結晶の色度(4
00nm波長の吸光度)は約25%減少した。それに対
し、カチオン性、アニオン性、両性界面活性剤では、晶
析時に添加しても得られた結晶の色度は改善されなかっ
た。
Table 1 shows the added surfactants and the obtained crystal properties. Ryoto Sugar Ester L which is a nonionic surfactant of sucrose fatty acid ester type
By adding WA-1540, the chromaticity (4
The absorbance at a wavelength of 00 nm was reduced by about 25%. In contrast, the cationic, anionic and amphoteric surfactants did not improve the chromaticity of the obtained crystals even when added during crystallization.
【0021】[0021]
【表1】 [Table 1]
【0022】実施例2 実施例1のグルタミン酸発酵液に95%硫酸を添加して
pH3.2にすることによりグルタミン酸結晶を析出さ
せた後に分離し、グルタミン酸含有母液を得た。この母
液を約2倍濃縮した後に10℃の恒温槽内で約50hr
冷却晶析し、結晶を分離した。分離した結晶は結晶重量
の約3倍量のグルタミン酸飽和水溶液で3回リスラリー
洗浄し、結晶に付着した母液のほとんどを洗い流した洗
浄結晶を得た。界面活性剤を添加するものについては、
グルタミン酸母液濃縮後に任意の量(対濃縮母液重量で
100、500、1000ppm)の界面活性剤を添加
した。
Example 2 Glutamic acid crystals were precipitated by adding 95% sulfuric acid to the glutamic acid fermented liquor of Example 1 to adjust the pH to 3.2, followed by separation to obtain a glutamic acid-containing mother liquor. After concentrating the mother liquor about twice, it is kept in a thermostat at 10 ° C. for about 50 hours.
The crystals were cooled and crystallized, and the crystals were separated. The separated crystals were reslurried and washed three times with a saturated aqueous solution of glutamic acid of about three times the weight of the crystals to obtain washed crystals in which most of the mother liquor attached to the crystals was washed away. For those adding a surfactant,
After concentration of the glutamic acid mother liquor, an arbitrary amount (100, 500, 1000 ppm by weight of the concentrated mother liquor) of a surfactant was added.
【0023】添加した界面活性剤と得られた結晶性状に
ついて表2に示す。ソルビタン脂肪酸エステルタイプの
ノニオン性界面活性剤であるノニオンST−221の添
加により、得られた結晶の色度(400nm波長の吸光
度)は約40%減少し、結晶平均粒径は約2倍に大型化
した。また、100ppmの添加量でも効果は見られた
が、500ppm以上添加した方が、より効果的であっ
た。
Table 2 shows the added surfactants and the obtained crystal properties. By the addition of nonionic ST-221, a nonionic surfactant of the sorbitan fatty acid ester type, the chromaticity (absorbance at a wavelength of 400 nm) of the obtained crystals is reduced by about 40%, and the average crystal grain size is about twice as large. It has become. In addition, although the effect was observed even with the addition amount of 100 ppm, it was more effective to add 500 ppm or more.
【0024】[0024]
【表2】 [Table 2]
【0025】実施例3 実施例1で得られたグルタミン酸発酵液に酸を添加する
ことにより得られた粗グルタミン酸α結晶40gとグル
タミン酸飽和溶液60gを混合し、グルタミン酸α結晶
スラリーを調製した。このスラリーに製品グルタミン酸
β結晶を0.4g添加し、90℃で3時間加温し、すべ
てのグルタミン酸α結晶を完全にβ結晶に転移させ、結
晶を分離した。分離した結晶は結晶重量の約3倍量のグ
ルタミン酸飽和水溶液で3回リスラリー洗浄し、結晶に
付着した母液のほとんどを洗い流した洗浄結晶を得た。
界面活性剤を添加するものについては、調製したグルタ
ミン酸α結晶スラリーに対スラリー重量で200ppm
添加した。
Example 3 Glutamic acid α crystal slurry was prepared by mixing 40 g of crude glutamic acid α crystal obtained by adding an acid to the glutamic acid fermented liquor obtained in Example 1 and 60 g of a glutamic acid saturated solution. 0.4 g of product glutamic acid β crystal was added to this slurry, and the mixture was heated at 90 ° C. for 3 hours to completely convert all glutamic acid α crystals to β crystals, thereby separating the crystals. The separated crystals were reslurried and washed three times with a saturated aqueous solution of glutamic acid of about three times the weight of the crystals to obtain washed crystals in which most of the mother liquor attached to the crystals was washed away.
As for the one to which the surfactant is added, the prepared glutamate α-crystal slurry is 200 ppm by weight based on the slurry weight.
Was added.
【0026】添加した界面活性剤と得られた結晶性状に
ついて表3に示す。ソルビタン脂肪酸エステルタイプの
ノニオン性界面活性剤であるノニオンOT−221添加
により、得られた結晶の色度(400nm波長の吸光
度)は約25%減少した。それに対し、アニオン性、両
性界面活性剤では、晶析時に添加しても得られた結晶の
色度は改善されなかった。
Table 3 shows the added surfactant and the obtained crystal properties. The addition of nonionic OT-221, a nonionic surfactant of the sorbitan fatty acid ester type, reduced the chromaticity (absorbance at a wavelength of 400 nm) of the obtained crystals by about 25%. On the other hand, with anionic and amphoteric surfactants, the chromaticity of the obtained crystals was not improved even when added during crystallization.
【0027】[0027]
【表3】 [Table 3]
【0028】[0028]
【発明の効果】本発明は、多くの色素物質及びタンパク
質等の不純物を含有する酸性アミノ酸発酵液から酸性ア
ミノ酸結晶を精製する際に、ある種のノニオン性界面活
性剤を100〜5000ppm添加することにより、酸
性アミノ酸結晶の収率を下げることなく、効果的に色素
の取り込みが少なく、大型の酸性アミノ酸結晶を取得す
ることが出来る。
According to the present invention, when purifying an acidic amino acid crystal from an acidic amino acid fermentation solution containing many pigment substances and impurities such as proteins, 100 to 5000 ppm of a certain nonionic surfactant is added. Thereby, a large amount of acidic amino acid crystals can be obtained with a small amount of dye incorporated effectively without lowering the yield of acidic amino acid crystals.

Claims (4)

    【特許請求の範囲】[Claims]
  1. 【請求項1】酸性アミノ酸の等電点晶析方法において、
    酸性アミノ酸含有液にノニオン性界面活性剤を添加し、
    晶析することを特徴とする酸性アミノ酸の晶析方法。
    1. A method for isoelectric crystallization of an acidic amino acid,
    Add a nonionic surfactant to the acidic amino acid-containing liquid,
    A method for crystallizing an acidic amino acid, comprising crystallization.
  2. 【請求項2】ノニオン性界面活性剤がソルビタン脂肪酸
    エステルタイプまたはショ糖脂肪酸エステルタイプの界
    面活性剤であることを特徴とする請求項1に記載の酸性
    アミノ酸の晶析方法。
    2. The method according to claim 1, wherein the nonionic surfactant is a sorbitan fatty acid ester type or sucrose fatty acid ester type surfactant.
  3. 【請求項3】ノニオン性界面活性剤を酸性アミノ酸含有
    液重量に対して100〜5000ppm添加することを
    特徴とする請求項1に記載の酸性アミノ酸の晶析方法。
    3. The method for crystallizing an acidic amino acid according to claim 1, wherein the nonionic surfactant is added in an amount of 100 to 5000 ppm based on the weight of the liquid containing the acidic amino acid.
  4. 【請求項4】酸性アミノ酸が、グルタミン酸であること
    を特徴とする請求項1に記載の酸性アミノ酸の晶析方
    法。
    4. The method for crystallizing an acidic amino acid according to claim 1, wherein the acidic amino acid is glutamic acid.
JP21618696A 1996-08-16 1996-08-16 Crystallization of acidic amino acid Pending JPH1059911A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP21618696A JPH1059911A (en) 1996-08-16 1996-08-16 Crystallization of acidic amino acid
PCT/JP1997/002780 WO1998007686A1 (en) 1996-08-16 1997-08-08 Method for crystallizing acidic amino acid
AU37847/97A AU3784797A (en) 1996-08-16 1997-08-08 Method for crystallizing acidic amino acid
IDP972841A ID18524A (en) 1996-08-16 1997-08-14 Methods for crystallizing amino acids are acidic

Publications (1)

Publication Number Publication Date
JPH1059911A true JPH1059911A (en) 1998-03-03

Family

ID=16684644

Family Applications (1)

Application Number Title Priority Date Filing Date
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Country Status (4)

Country Link
JP (1) JPH1059911A (en)
AU (1) AU3784797A (en)
ID (1) ID18524A (en)
WO (1) WO1998007686A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099426A1 (en) * 2003-05-07 2004-11-18 Ajinomoto Co., Inc. Process for producing l-glutamic acid
JPWO2013039094A1 (en) * 2011-09-12 2015-03-26 協和発酵バイオ株式会社 Amino acid production method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4619610B1 (en) * 1966-08-25 1971-06-01
EP0410005B1 (en) * 1989-02-13 1995-12-27 Ajinomoto Co., Inc. Method for crystallizing amino acid

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099426A1 (en) * 2003-05-07 2004-11-18 Ajinomoto Co., Inc. Process for producing l-glutamic acid
US7294491B2 (en) 2003-05-07 2007-11-13 Ajinomoto Co., Inc. Method for producing L-glutamic acid
CN100383252C (en) * 2003-05-07 2008-04-23 味之素株式会社 Process for producing l-glutamic acid
JPWO2013039094A1 (en) * 2011-09-12 2015-03-26 協和発酵バイオ株式会社 Amino acid production method

Also Published As

Publication number Publication date
WO1998007686A1 (en) 1998-02-26
AU3784797A (en) 1998-03-06
ID18524A (en) 1998-04-16

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