JPH1017310A - Collagen, production of hydroxyapatite and its product - Google Patents

Collagen, production of hydroxyapatite and its product

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Publication number
JPH1017310A
JPH1017310A JP17330296A JP17330296A JPH1017310A JP H1017310 A JPH1017310 A JP H1017310A JP 17330296 A JP17330296 A JP 17330296A JP 17330296 A JP17330296 A JP 17330296A JP H1017310 A JPH1017310 A JP H1017310A
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JP
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Patent type
Prior art keywords
hydroxyapatite
collagen
products
immersing
soln
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17330296A
Other languages
Japanese (ja)
Inventor
Kazuko Hirayama
和子 平山
Original Assignee
Ehime Pref Gov
愛媛県
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Abstract

PROBLEM TO BE SOLVED: To establish the process for production of hydroxyapatite and non- denatured collagen and to effectively utilize the products by using bones easily available as the by-products of meat industry as a starting raw material. SOLUTION: The feet of broilers finely crushed by a crushing machine are subjected to a pretreatment by a method for washing these legs with water and <=0.5N aq. sodium chloride soln., then immersing the feet into 0.1N aq. sodium hydroxide soln. The pretreated raw materials are then immersed into acetic acids of various concns. and the immersing liquid is neutralized and the precipitate is subjected to freeze drying, by which the hydroxyapatite and the fibrous non-denatured collagen are obtd. from the bones.

Description

【発明の詳細な説明】 DETAILED DESCRIPTION OF THE INVENTION

【0001】 [0001]

【産業上の利用分野】本発明は骨を出発原料とするコラーゲン、ヒドロキシアパタイトの製造法及び前記製造法によって得られる未変性コラーゲン、ヒドロキシアパタイトの有効利用に関するものである。 The present invention relates is related to the effective use of non-denatured collagen, hydroxyapatite obtained by collagen, hydroxyapatite preparation and the preparation of a starting material of bone.

【0002】 [0002]

【従来の技術】食鳥(ブロイラー)業の大規模化につれて大量に排出される鶏の足は、一部が食材として輸出される以外は、インスタントラーメンのスープ用鳥ガラやブロイラー用飼料の原料としてしか使用されていない。 Leg of chicken, which is a large amount discharged as the scale of the Background of the Invention poultry (broiler) industry, except that part of it is exported as food, raw materials of soup for birds gala and broiler feed for instant noodles not being used only as.
また骨を構成するコラーゲンはゼラチンとして抽出されるだけで、コラーゲンの製造には牛及び豚の皮が使われているだけである。 The collagen that constitutes the bone only be extracted as gelatin, the production of collagen are only being used skins of cattle and pigs.

【0003】 [0003]

【発明が解決しようとする課題】本発明は、食肉産業の副産物として容易に入手することができる骨からコラーゲン及びヒドロキシアパタイトを抽出し、食肉産業の副産物を有効利用することを目的としている。 [0008] The present invention can be easily obtained as a by-product of the meat industry to extract collagen and hydroxyapatite from the bone, and intended to effectively utilize the by-products of the meat industry.

【0004】 [0004]

【課題を解決するための手段】上記の目的を達成するために、本発明の方法は、皮や身がついたままの鶏等の足を破砕機で細砕し、塩化ナトリウム、水酸化ナトリウム水溶液で前処理をした後、種々の濃度の酢酸に浸漬、浸漬液を中和することで得られる析出物を凍結乾燥することによって、骨からヒドロキシアパタイトと繊維状の未変性コラーゲンを得るものである。 To achieve the above object, according to an aspect of the method of the present invention, comminuted feet chicken etc. remain attached skin or body at crusher, sodium chloride, sodium hydroxide after the pre-treated with an aqueous solution, immersed in various concentrations of acetic acid, by lyophilizing the precipitate obtained by neutralizing the immersion liquid, so as to obtain native collagen hydroxyapatite and fibrous bone is there.

【0005】 [0005]

【作用及び発明の効果】上記製造方法により、前処理で用いた塩化ナトリウム、水酸化ナトリウムは骨や皮に付着している血液や脂質、タンパク質に作用し、コラーゲン、ヒドロキシアパタイトを抽出する際の不純物を取り除くことができる。 The above manufacturing method, according to the action and the present invention, sodium chloride was used in the pretreatment, blood and lipids sodium hydroxide is attached to the bones and skin, it acts on the protein, in extracting collagen, hydroxyapatite it is possible to remove the impurities.

【0006】次に、コラーゲンとヒドロキシアパタイトを個々に抽出するために酸の種類や酸濃度について検討した。 [0006] Next, we investigated the type and acid concentration of the acid in order to extract collagen and hydroxyapatite individually. その結果、安価な酸の中で、コラーゲンを変性させることなく効率よく抽出するには酢酸がもっとも適当であった。 As a result, in a low-cost acid, to efficiently extract without denaturing the collagen acetate was the most suitable. 0.5Nより薄い濃度の酢酸を用いることで、ヒドロキシアパタイトだけを溶出させることができた。 By using a low concentration of acetic acid from 0.5 N, it could be eluted by hydroxyapatite. なおヒドロキシアパタイトを溶出させた後、酢酸の濃度を上げることでコラーゲンだけを抽出することが可能となった。 Note After elution hydroxyapatite, it was possible to extract only the collagen by raising the concentration of acetic acid.

【0007】得られたヒドロキシアパタイトは900 ℃で1時間加熱しても無煙、無臭で、減量率も公知の方法で製造する骨灰より少なかった。 [0007] The obtained hydroxyapatite smokeless be heated 1 hour at 900 ° C., odorless, it was less than bone ash that weight loss also be produced by known methods. またこの方法で製造した骨灰を原料にした骨灰磁器は市販のものより高い白色度を有していた。 The bone china where the bone ash produced in this process the raw material had high whiteness than commercially available.

【表1】 [Table 1]

【表2】 [Table 2]

【0008】マウスを用いた実験から、従来のものより骨へのカルシウム吸収性が高かった。 [0008] Experiments using mice was higher calcium absorption into bone than conventional.

【0009】未変性コラーゲンは繊維状のものを得ることができ、パルプとの配合紙への利用が可能となった。 [0009] Native collagen can get what fibrous, has become possible to use in the formulations paper pulp.
抄紙した配合紙はコラーゲン配合率が高くなるほど吸油性が向上した。 Paper was compounded sheet was improved more oil absorbing collagen mixture ratio becomes higher.

【図1】 [Figure 1]

【0010】 [0010]

【実施例1】本発明は下記の実施例によってさらに具体的に説明されるが、本発明はこれらに限定されるものではない。 EXAMPLE 1 The present invention is by the examples more specifically described below, the present invention is not limited thereto.

【0011】ブロイラーの足を破砕機で細砕し、水で洗浄し、さらに0.5N以下の濃度の塩化ナトリウム水溶液で3回以上洗浄後、0.1Nの水酸化ナトリウム水溶液中に懸濁させ、24時間以上緩やかに撹拌する。 [0011] The foot of broilers was comminuted in a crusher, and washed with water, further washed three times or more at the following concentrations of aqueous sodium chloride solution 0.5 N, was suspended in 0.1N sodium hydroxide aqueous solution, 24 gently stirred for more than an hour.

【0012】次いで固体を0.1Nの酢酸中に懸濁させ、24 [0012] then suspended solid in 0.1N acetic acid, 24
時間以上放置する。 Allowed to stand for more than an hour. この物質を5Aのろ紙でろ過、液相を水酸化ナトリウムで中和してヒドロキシアパタイトを沈殿させ、蒸留水で洗浄してから回収した。 Filtration of this material in filter paper 5A, precipitated hydroxyapatite by neutralizing the liquid phase with sodium hydroxide, was collected was washed with distilled water.

【0013】再度抽出残渣を0.1N酢酸で抽出し、ヒドロキシアパタイトが完全に分離するまで操作を繰り返した。 [0013] extracts re-extracted residue with 0.1N acetic acid, hydroxyapatite was repeated until complete separation.

【0014】次にヒドロキシアパタイトの抽出残渣を0. [0014] Next, extraction residue of hydroxyapatite 0.
5N以上の酢酸に懸濁させ、24時間以上放置した。 5N suspended above the acetic acid, and allowed to stand for 24 hours or more. この物質を5Aのろ紙でろ過し、液相を収集し、固体を回収し、 The material was filtered through a 5A filter paper, collect the liquid phase and the solid was collected,
水酸化ナトリウムを前記液相に添加してコラーゲンを析出させ、これを36/32の透析膜に定量的に移し、蒸留中で透析を行った。 Was added sodium hydroxide to the liquid phase to precipitate collagen, which quantitatively transferred to a dialysis membrane of 36/32, it was dialyzed in distilled.

【0015】このコラーゲンをバットに広げ、凍結乾燥を行い、繊維状のコラーゲンを得た。 [0015] The spread of this collagen to bat, and then freeze-dried to obtain a fibrous collagen.

【0016】 [0016]

【実施例2】実施例1で得られた沈殿を凍結乾燥し、粉末状のヒドロキシアパタイトを得た。 EXAMPLE 2 The precipitate obtained in Example 1 was freeze-dried to obtain a powdery hydroxyapatite. これを900℃で1 This 1 at 900 ℃
時間加熱して骨灰とし、骨灰、セリサイト粘土、長石、 And bone ash by heating time, bone ash, sericite clay, feldspar,
カオリンを重量比で4:3:2:1の割合で配合し、ポットミルによって湿式混合摩砕、生素地とした。 4 kaolin in a weight ratio: 3: 2: in proportions of 1, were wet mixed grinding, the greenware by a pot mill. 成形後、1200 After molding, 1200
℃の酸化炎雰囲気で焼成することにより、骨灰磁器素地を得た。 By firing in an oxidizing flame atmosphere ° C., to obtain a bone china basis material.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

【図1】未変性コラーゲンを抄紙した配合紙のコラーゲン配合率による吸油性を示す図である。 1 is a diagram showing an oil absorbing by Collagen rate of compounding paper and papermaking native collagen.

Claims (5)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】 骨からヒドロキシアパタイトを製造する方法において、 (a)ヒドロキシアパタイトを希薄酢酸で溶出する工程、 (b)溶出液をアルカリ溶液で中和してヒドロキシアパタイトを沈殿させる工程、 (c)沈殿物を凍結乾燥して粉末のヒドロキシアパタイトを得る工程を有するヒドロキシアパタイトの製造方法。 1. A process for preparing hydroxyapatite from the bone, (a) a step of eluting the hydroxyapatite dilute acetic acid, precipitating hydroxyapatite and neutralized with an alkali solution (b) eluate, (c ) the method of producing the precipitate was freeze-dried hydroxyapatite having a step of obtaining a hydroxyapatite powder.
  2. 【請求項2】 請求項1(a)の残渣から未変性コラーゲンを製造する方法において、 (a)コラーゲンを酢酸または希薄酢酸で抽出する工程、 (b)抽出液をアルカリ溶液で中和することでコラーゲンを析出させる工程、 (c)析出物を凍結乾燥して繊維状のコラーゲンを得る工程を有する未変性コラーゲンの製造方法。 2. A method for producing a native collagen from the residue of claims 1 (a), (a) a step of extracting collagen with acetic acid or dilute acetic acid, neutralization with an alkali solution (b) extract step of precipitating the collagen in method of native collagen having a step of obtaining a fibrous collagen was lyophilized to (c) precipitates.
  3. 【請求項3】 出発原料として鶏の足を使用する請求項1または2記載の方法。 3. A method according to claim 1 or 2 for use foot chicken as a starting material.
  4. 【請求項4】 請求項1に記載の方法によって製造されたヒドロキシアパタイトの骨灰磁器もしくはカルシウム補給用食品への使用。 4. The use of the bone china or calcium supplementation for food hydroxyapatite produced by the method of claim 1.
  5. 【請求項5】 請求項2に記載の方法によって製造されたコラーゲンの紙もしくは化粧品への利用。 5. The use of the paper or cosmetic collagen produced by the method of claim 2.
JP17330296A 1996-07-03 1996-07-03 Collagen, production of hydroxyapatite and its product Pending JPH1017310A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Publications (1)

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JPH1017310A true true JPH1017310A (en) 1998-01-20

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100871396B1 (en) 2007-02-23 2008-12-02 재단법인서울대학교산학협력재단 Method for Manufacturing Bioactive Apatite
JP2009269930A (en) * 2001-09-14 2009-11-19 Cytori Therapeutics Inc Preservation of non-embryonic cell from non-hematopoietic tissue
US8691216B2 (en) 2001-12-07 2014-04-08 Cytori Therapeutics, Inc. Methods of using regenerative cells to promote wound healing
EP2783707A1 (en) * 2011-11-23 2014-10-01 Chungbuk National University Industry-Academic Cooperation Foundation Production method for biomedical and industrial material using ceramic derived from birds' beaks
US8883499B2 (en) 2001-12-07 2014-11-11 Cytori Therapeutics, Inc. Systems and methods for isolating and using clinically safe adipose derived regenerative cells
US9133431B2 (en) 2009-05-01 2015-09-15 Bimini Technologies Llc Systems, methods and compositions for optimizing tissue and cell enriched grafts
US9198937B2 (en) 2001-12-07 2015-12-01 Cytori Therapeutics, Inc. Adipose-derived regenerative cells for treating liver injury
US9463203B2 (en) 2001-12-07 2016-10-11 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of cartilage defects
US9480718B2 (en) 2001-12-07 2016-11-01 Cytori Therapeutics, Inc. Methods of using adipose-derived regenerative cells in the treatment of peripheral vascular disease and related disorders
US9486484B2 (en) 2008-08-19 2016-11-08 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009269930A (en) * 2001-09-14 2009-11-19 Cytori Therapeutics Inc Preservation of non-embryonic cell from non-hematopoietic tissue
US9504716B2 (en) 2001-12-07 2016-11-29 Cytori Therapeutics, Inc. Methods of using adipose derived regenerative cells to promote restoration of intevertebral disc
US8691216B2 (en) 2001-12-07 2014-04-08 Cytori Therapeutics, Inc. Methods of using regenerative cells to promote wound healing
US9849149B2 (en) 2001-12-07 2017-12-26 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of erectile dysfunction
US8883499B2 (en) 2001-12-07 2014-11-11 Cytori Therapeutics, Inc. Systems and methods for isolating and using clinically safe adipose derived regenerative cells
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US9511096B2 (en) 2001-12-07 2016-12-06 Cytori Therapeutics, Inc. Methods of using regenerative cells to treat an ischemic wound
US9198937B2 (en) 2001-12-07 2015-12-01 Cytori Therapeutics, Inc. Adipose-derived regenerative cells for treating liver injury
US9463203B2 (en) 2001-12-07 2016-10-11 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of cartilage defects
US9480718B2 (en) 2001-12-07 2016-11-01 Cytori Therapeutics, Inc. Methods of using adipose-derived regenerative cells in the treatment of peripheral vascular disease and related disorders
US9511094B2 (en) 2001-12-07 2016-12-06 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of stroke and related diseases and disorders
US9492483B2 (en) 2001-12-07 2016-11-15 Cytori Therapeutics, Inc. Methods of using regenerative cells to treat a burn
US9872877B2 (en) 2001-12-07 2018-01-23 Cytori Therapeutics, Inc. Methods of using regenerative cells to promote epithelialization or neodermis formation
KR100871396B1 (en) 2007-02-23 2008-12-02 재단법인서울대학교산학협력재단 Method for Manufacturing Bioactive Apatite
US9486484B2 (en) 2008-08-19 2016-11-08 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
US9133431B2 (en) 2009-05-01 2015-09-15 Bimini Technologies Llc Systems, methods and compositions for optimizing tissue and cell enriched grafts
EP2783707A4 (en) * 2011-11-23 2015-04-08 Nat Univ Chungbuk Ind Acad Production method for biomedical and industrial material using ceramic derived from birds' beaks
EP2783707A1 (en) * 2011-11-23 2014-10-01 Chungbuk National University Industry-Academic Cooperation Foundation Production method for biomedical and industrial material using ceramic derived from birds' beaks

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