JPH0798314A - Feces occult blood measuring device - Google Patents

Abstract

PURPOSE:To provide a device capable of readily measuring the existence of feces occult blood with a simple structure by immunization.. CONSTITUTION:A feces collecting apparatus having collected a feces sample is inserted through the opening 1a of a buffer solution container 1 storing a buffer solution 4 to obtain a feces sample dissolved liquid 4A. A measuring cover body 5 fixed with a separating filter 6 for removing a solid matter and a hemoglobin antibody and arranged with a measuring filter 7 impregnated with colored latex is liquid-tightly fitted to the opening 1a of the buffer solution container 1. When the buffer solution container 1 is reversed so that the measuring cover body 5 is located on the lower side, the feces sample dissolved liquid 4A drips to the measuring filter 7 side, and the existence of feces occult blood is judged in this feces occult blood measuring device.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a simple fecal occult blood measuring apparatus for determining the presence or absence of fecal occult blood, and more particularly to a fecal occult blood measurement for determining the presence or absence of fecal occult blood by an immunoassay using an anti-human hemoglobin antibody. Regarding the device.

[0002]

2. Description of the Related Art Fecal occult blood, that is, blood mixed in feces, is a strong indicator of digestive system diseases and is therefore widely used for examination of digestive system diseases. As the fecal occult blood test method, there are a chemical method using the peroxidase-like activity of hemoglobin Hb in blood and an immunization method using an anti-human Hb antibody.

As a fecal occult blood measuring apparatus utilizing a chemical method, a card type simple measuring apparatus has been proposed, as disclosed in, for example, Japanese Patent Publication No. 2-45826. Here, a test paper containing a filter paper impregnated with guaiac butter and a coloring reagent (hydrogen peroxide solution) are used. In use, a stool sample is applied on a test paper, a coloring reagent is further dropped on the stool sample, and the presence or absence of fecal occult blood is determined by the discoloration of guaiac fat.

On the other hand, in the above-mentioned immunization method, a monoclonal antibody against human Hb is immobilized on a carrier such as latex particles, and an agglutination image expressed by an antigen-antibody reaction performed with Hb in a stool sample is observed. The presence or absence of fecal occult blood is determined. A method for measuring fecal occult blood using such an immunization method is disclosed, for example, in JP-A-59-125064.
It is disclosed in Japanese Patent Laid-Open No. 2-141665.

[0005]

In the above chemical method, a simple fecal occult blood measuring system has been established, but since the chemical method does not utilize a specific reaction, fecal occult blood is easily affected by food and drugs. There was a drawback that the measurement sensitivity was low.

On the other hand, the immunization method utilizes a specific reaction and is therefore unlikely to be affected by plants and drugs. However, there is a problem that skill is required to determine the aggregated image. In addition, the measuring instrument using the immunization method has a complicated configuration,
For example, the latex method has a problem that it requires various instruments such as a judgment plate, a reagent, and a stirring rod.

An object of the present invention is to provide a fecal occult blood measuring apparatus capable of measuring fecal occult blood simply without using a large number of instruments and without requiring skill, despite using the immunization method. To do.

[0008]

The present invention is an apparatus for measuring the presence or absence of fecal occult blood by the immunization method as described above, and achieves the above object by including the following configuration.

That is, according to the first aspect of the invention, a buffer solution container for storing a buffer solution for dissolving a stool sample and having an opening, and a stool container inserted into the buffer solution container through the opening are provided. A stool collection tool for immersing a sample in a buffer solution, and a lid for measurement that is detachably attached to the opening of the buffer solution container in a liquid-tight manner, and the lid for measurement includes a stool sample. Is provided with an outlet for flowing out the dissolved stool sample solution, and the solid content in the stool sample solution is removed between the side attached to the opening of the buffer container and the outlet. The apparatus for measuring fecal occult blood is characterized in that the separation filter and the measurement filter fixed with anti-human hemoglobin antibody and impregnated with colored latex are arranged in this order.

Further, the invention according to claim 2 is such that a buffer solution for dissolving a stool sample is contained therein and has an opening, and the opening of the buffer solution container is liquid-tight and A detachable lid is attached to the lid, and a rod-shaped stool collection tool provided so as to extend into the buffer solution when attached to the buffer container is attached to the lid sample. Is provided with an outlet for flowing out the dissolved buffer solution, and the lid body is provided with a solid content in the stool sample solution between the side attached to the opening of the buffer solution container and the outlet. The apparatus for measuring fecal occult blood is characterized in that a separation filter for removing urine and a measurement filter fixed with an anti-human hemoglobin antibody and impregnated with colored latex are arranged in this order.

The structures of the inventions according to claims 1 and 2 will be described below. In the invention described in claim 1, in the invention described in claim 1, as described above, the buffer container,
Only three instruments, a stool collection instrument and a measuring lid, are used.

As the buffer solution container, one having an appropriate shape can be used as long as it can accommodate a buffer solution for dissolving a stool sample. However, the buffer container is partially provided with an opening as described above.

The buffer container is made of polyethylene,
Although it is composed of an appropriate synthetic resin such as polypropylene or vinyl chloride resin, the material forming the buffer solution container is not particularly limited. Although the capacity of the buffer solution container is not particularly limited, it may be 1 to 10 m so as to enable measurement with a measurement lid described later.
It is preferably about l.

The buffer solution contained in the buffer solution container is
For example, a neutral salt solution such as an ammonia buffer solution, a glycine buffer solution, a phosphate buffer solution or a Tris buffer solution can be appropriately used, and the pH is preferably in the range of 6.5 to 8.5. .

The buffer container is provided with an opening as described above, and this opening is used for inserting a stool collection device.
In addition, it is provided for liquid-tightly and detachably attaching the measurement lid. The lid for measurement needs to be liquid-tightly attached to the opening, but an appropriate structure can be adopted as long as it can be attached liquid-tightly. For example,
A method of forming a screw around the opening of the buffer solution container and the measuring lid, or a structure in which the measuring lid is crimped to the inner peripheral surface of the opening of the buffer container or the outer surface of the portion where the opening is provided. Can be adopted.

[0016] Preferably, the stool collecting device may also be provided with a lid, and the lid of the stool collecting device may be detachably and liquid-tightly attached to the opening of the buffer solution container.
In that case, since the opening is closed by the lid provided in the stool collection device in a state where a part of the stool collection device is immersed in the buffer solution, simply shaking the buffer solution container by hand, The stool sample can be dissolved in the buffer.

As the above-mentioned stool collection device, as long as it has a shape to be inserted from the opening of the buffer solution container and immersed in the buffer solution,
Although a device having an appropriate structure can be used, it is preferable to use a stool collection device which is substantially rod-shaped as a whole and has a stool collection part at its tip. The stool collection part is formed by forming irregularities, depressions, holes or protrusions on the outer surface of the stool collection device, and can collect stool in an amount corresponding to the volume of the irregularities, depressions or holes.

Further, as described above, preferably, the stool collecting device is also provided with a lid for liquid-tightly closing the opening of the buffer solution container. In this case, it is preferable to use, as the stool collection device, a substantially rod-shaped stool collection device body attached so as to extend into the buffer solution from the inner surface of the lid.

The material for forming the stool collection device is not particularly limited, and synthetic resins such as polypropylene and vinyl chloride resin can be used. As described above, the measurement lid is liquid-tightly and detachably attached to the opening of the buffer container. The material of the measuring lid is also not particularly limited, and for example, synthetic resin such as polyethylene, polypropylene, or vinyl chloride resin can be used.

The measuring lid is provided with an outlet, which is provided for discharging the fecal sample solution that has flowed out through a separation filter and a measuring filter described later.

The separation filter is provided to remove unnecessary solids in the stool sample solution, and can be composed of a known filter paper or a membrane or filter composed of nitrocellulose fiber. The separation filter is
As described above, it is arranged between the side of the buffer container attached to the opening and the outlet of the measurement lid, on the side of the measurement filter attached to the opening. This is because the insoluble solid content in the stool sample solution is removed before the stool sample solution is supplied to the measurement filter.

The measurement filter is provided for measuring the presence or absence of fecal occult blood in the stool sample solution by the immunoassay,
When fecal occult blood is present, the measurement filter is colored in a predetermined color tone due to an immune reaction described later.

That is, the above-mentioned measurement filter is provided to carry out a known immunological filter, and utilizes a latex agglutination reaction. In the latex agglutination reaction method, for example, an antigen-antibody reaction between an antibody-sensitized latex and an antigen causes an immune reaction product to form a network structure to generate an aggregate. This immunoreaction is carried out on a filter having a large number of narrow mouths through which the above-mentioned aggregates cannot pass but non-aggregates can pass, so that the presence or absence of latex on the filter after the reaction determines positive or negative. . At the time of determination, preferably, the color tone of the carrier has a clear contrast with the color tone of the filter, whereby the determination can be made more reliable and easy. In the present invention, the above-mentioned antigen is human Hb in the stool sample solution.
And the antibody is an anti-human Hb antibody.

Further, in the present invention, the anti-human Hb antibody is obtained by purifying high serum obtained by immunizing rabbits, sheep, goats, horses, guinea pigs, etc. with human Hb by affinity chromatography or the like. To be Further, it is also possible to produce a monoclonal antibody by a known cell fusion method using immunized mouse spleen cells. In any case, it is desirable that the anti-human Hb antibody used be sufficiently purified.

The method of sensitizing the anti-human Hb antibody to the carrier, that is, the method of immobilizing it on the latex can be carried out by a known method such as a separation adsorption method or a chemical bonding method. For example, the anti-human Hb antibody can be physically adsorbed on the latex surface by adding a buffer solution of the anti-human Hb antibody to the latex and heating the mixture at 37 ° C. for 30 to 120 minutes with stirring. Further, if a latex having a carboxyl group is used, the anti-human Hb antibody can be bound to the latex by covalent bond via a water-soluble carbodiimide or the like. If necessary, bovine serum albumin (hereinafter abbreviated as BSA) and the like may be added.

Further, in the present invention, a colored carrier, that is, a colored latex is used in order to make it different from the color tone of the carrier, the background of the portion where the carrier is present, and the color tone of the agglomerated image. Latex particles are usually white, but for example, latex particles obtained by emulsion polymerization of styrene, vinyltoluene, etc., in a reaction medium containing water and an organic solvent compatible with water, in the reaction medium. And a latex particle obtained by emulsion polymerization having an anionic functional group, for example, a copolymer containing a monomer having an anionic functional group such as (meth) acrylic acid. The latex particles made of a polymer can be obtained by, for example, a method of contacting with a colorant which is an amphoteric electrolyte dissolved in the reaction medium in an aqueous reaction medium to color the latex particles.

In the present invention, the measurement latex is impregnated with the above-mentioned colored latex, but a second anti-human Hb antibody (hereinafter abbreviated as second antibody) is directly fixed to the measurement filter. That is, the second antibody having a different epitope from the human Hb antibody or lower (hereinafter abbreviated as the first antibody) immobilized on the colored latex is directly immobilized on the measurement filter. As the immobilization method, a physical adsorption method, a chemical bonding method, or the like can be appropriately used. For example, 10 to 500 μg / ml second
An example is a method in which 50 to 1000 μl of the antibody is dropped onto the measurement filter, left standing at room temperature for 12 hours or longer, and then BSA or the like is dropped onto the measurement filter or the like if necessary and dried.

The second antibody has a different epitope from that of the first antibody, and the second antibody can be obtained in the same manner as the above-mentioned first antibody. Also,
The measurement filter can be composed of any material as long as it is a porous material through which unreacted colored latex can pass, for example, a cellulose-based comb or a synthetic resin-based porous material. . The average fine diameter of the porous material varies depending on the coloring latex described later to be used, but is preferably about 0.1 to 2 μm.

In the present invention, the first antibody fixed to the colored latex and the second antibody directly fixed to the measurement filter cause an antigen-antibody reaction with the antigen, that is, human Hb, and human Hb-first antibody. The sensitized carrier (colored latex) complex is trapped in the second antibody immobilized on the measurement filter. That is, the sandwich method forms an immune complex. Therefore, the stool sample solution is dropped on the measurement filter, and when fecal occult blood is present, an immune complex is formed by the above-mentioned immune reaction. On the other hand, in the absence of fecal occult blood, the immune complex is not formed. Therefore, fecal occult blood can be measured depending on whether or not the measurement filter is colored depending on whether or not the immune complex is formed.

Invention of Claim 2 The fecal occult blood measuring apparatus according to the invention of Claim 2 has a buffer solution container and a lid as described above. The buffer solution container and the buffer solution contained therein are configured similarly to the invention described in claim 1.

The lid body is provided with a rod-shaped stool collection tool that extends into the buffer solution when attached to the buffer solution container. The rod-shaped stool collection device itself is configured similarly to the stool collection device used in the first aspect of the invention.

Further, in the present invention, in the above lid,
In addition to the stool collection device, an outlet for letting out a stool sample solution is formed, and a separation filter and a measurement filter are arranged. The outlet, the separation filter, and the measurement filter are configured in the same manner as the measurement lid in the first aspect of the invention.

Therefore, the lid according to the invention described in claim 2 corresponds to a combination of the lid for measurement and the stool collection device according to the invention described in claim 1. Therefore, claim 2
The parts of the lid in the invention described in 1) will be omitted by referring to the description of the measurement lid described in the invention in claim 1.

[0034]

In the invention described in claim 1, only three kinds of members which are prepared for measuring fecal occult blood are a buffer solution container, a stool collection instrument and the measurement lid, and In the described invention, fecal occult blood is determined using only two types of members, the buffer solution container and the lid.

In the case of using the fecal occult blood measuring apparatus according to the first aspect of the present invention, first, a fecal sampling instrument is inserted through the opening of the buffer solution container to dissolve the fecal sample, and then the measurement lid is placed in the buffer solution. Attach so that the opening of the container is closed. After that,
All operations are completed simply by reversing the buffer container and allowing the stool sample solution to flow down from the opening to the outlet side of the measurement lid. For the determination, it is only necessary to allow the fecal sample solution to flow down from the outlet, and then determine whether or not the measurement filter is colored. Therefore, it is possible to easily determine the presence or absence of fecal occult blood.

In the invention described in claim 2, only two members, the buffer solution container and the lid, are prepared, and a stool sample is collected in a stool collection tool provided on the lid, and then the lid is removed. All the operations are completed by attaching the buffer solution container to the buffer solution container, then reversing the buffer solution container, and allowing the stool sample solution to flow down to the outlet side of the lid. Therefore, it is possible to more easily determine the presence or absence of fecal occult blood as compared with the invention according to claim 1.

[0037]

As described above, according to the first aspect of the present invention, the presence or absence of fecal occult blood can be easily determined simply by preparing the three members of the buffer solution container, the stool collection instrument and the measurement lid. According to the second aspect of the present invention, it is possible to determine the presence or absence of fecal occult blood by preparing only two members, the buffer solution container and the lid, and performing a simpler operation.

Moreover, in the inventions according to claims 1 and 2,
Since the measurement is performed by the immunization method, the presence or absence of fecal occult blood can be determined more reliably without being affected by plants or drugs.

[0039]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be clarified by describing embodiments with reference to the drawings.

Example 1 FIG. 1 (a) is a cross-sectional view showing a state in which a stool collection device is inserted in a buffer container, and FIG. 1 (b) shows a state in which a stool sample is collected in the stool collection device. It is a perspective view.

In this embodiment, first, the buffer container 1 and the stool collection device 2 are prepared. The buffer solution container 1 is made of a synthetic resin such as polypropylene, polyethylene, or vinyl chloride resin, and has an opening 1a at the top.

On the other hand, the stool collection device 2 has a rod-shaped stool collection device body 2a and a lid 2b. Rod-shaped stool collection device body 2a
A concave portion 2c is formed on the outer surface near the lower end of the
A stool sample 3 is collected in the recess 2c. The stool sample 3 is collected by piercing the stool collection device body 2a into the stool and pulling out the stool collection device body 2a from the stool. By this operation, the stool sample 3 is collected in an amount corresponding to the shape of the recess 2c.

A lid 2b is integrally provided on the upper end of the rod-shaped feces collecting tool body 2a. The lid body 2b is configured to be liquid-tightly fixed to the outer peripheral wall 1b of the portion where the opening 1a is formed after inserting the stool collection device body 2a from the opening 1a of the buffer solution container 1. That is, as shown in FIG. 1A, even if the buffer solution container 1 is shaken to dissolve the stool sample 3 in a state where the stool collection device 2 is attached to the buffer solution container 1, the internal buffer solution is shaken. The lid body 2b is liquid-tightly fixed to the buffer solution container 1 so as not to leak. In this embodiment, the liquid-tight fixing is configured such that the inner peripheral wall of the lid body 2b has a slightly smaller diameter than the outer peripheral wall 1b of the portion where the opening 1a is formed, and the lid 2b is fixed. This is performed by press-fitting the outer peripheral wall 1b of the buffer solution container 1 inside. However, it is also possible to form a screw on both of them and fix them, thereby achieving liquid-tight fixing.

In the fecal occult blood analyzer of the present embodiment, after the fecal sample has been collected as described above, the feces collection device 2 is attached to the buffer container 1, and as shown in FIG. The stool sample is dissolved in the buffer solution 4 by grasping and shaking. Then, the stool collection device 2 is removed from the buffer solution container 1.

Next, as shown in FIG. 3, in the fecal occult blood measuring apparatus of this embodiment, the measuring lid 5 is attached to the buffer solution container 1. The measurement lid 5 has a mounting portion 5a on the lower end side, as shown in an enlarged manner in FIG. Mounting part 5a
The inner diameter of the outer peripheral wall 1b is slightly smaller than that of the outer peripheral wall 1b, and the outer peripheral wall 1b is press-fitted into the mounting portion 5a so that they are fixed in a liquid-tight manner.

In the measurement filter 5, the outlet 5 is provided on the upper end side.
b is formed. The outflow port 5b is provided for flowing down the fecal sample solution as described later. Then, the mounting portion 5a side, that is, the opening 1 in FIG.
The separation filter 6 and the measurement filter 7 are arranged in this order between the a side and the downflow port 5b. In the fecal occult blood measuring apparatus of the present embodiment, the fecal sample is dissolved in the buffer solution 4 as described above to obtain the fecal sample solution 4A, and then the measurement lid 5 is attached to the buffer solution container 1. After that, FIG. 5 (a)
As shown in, the buffer solution container 1 is reversed and the fecal sample solution 4
A is made to flow down from the outlet 5b. In this case, FIG. 5 (b)
As shown in, the solid content of the stool sample solution 4A is first removed by the separation filter 6. Then, the stool sample solution from which the solid content has been removed is dropped on the measurement filter 7.
On the measurement filter 7, when fecal occult blood is present, as shown in FIG.
The second antibody reacts with each other to form the immune complex 8. As a result, the measurement filter is colored. On the other hand, when there is no fecal occult blood, the measurement filter 7 is in a non-colored state.
Therefore, the presence or absence of fecal occult blood can be easily determined by visually determining whether or not the measurement filter 7 is colored.

The stool sample solution that has passed through the measurement filter 7 flows out through the outlet 5b. As described above, in the present embodiment, the presence or absence of fecal occult blood can be easily determined by the immunization method simply by preparing the three members of the buffer solution container 1, the stool collection device 2 and the measurement lid 5. .

Although the stool collection device 2 is provided with the lid 2b in the above embodiment, the stool collection device may not have the lid 2b. That is, the dissolution of the collected stool sample in the buffer solution 4 does not necessarily have to be performed with the lid body 2b fixed as shown in FIG. 2, and a rod-shaped stool collection tool may be immersed in the buffer solution container 4. The stool sample may be dissolved by stirring the buffer solution 4 with a rod-shaped stool collection tool. However, as in the above embodiment, when the lid 2b is provided on the stool collection device 2, the stool collection device 2 is inserted into the buffer solution container 1,
As shown in FIG. 2, the stool sample can be dissolved simply by shaking the whole, which is preferable.

It should be noted that the structure shown in the figure is merely an example, and the shapes of the individual members are defined in claim 1.
It can be appropriately modified within a range that does not impair the object of the invention described in (1). For example, the shape and number of the recesses 2b provided in the stool collection device 2 are not particularly limited, and the shape of the stool collection device body 2a can be changed to another shape such as a prismatic shape.

Example 2 FIGS. 7 (a) and 7 (b) are a sectional view and a perspective view showing a lid for explaining a fecal occult blood measuring apparatus of Example 2. As shown in FIG.

In this embodiment, the buffer solution container 11 and the lid 1 are
Two members 2 and 2 are prepared. In addition, in FIG. 7A, a cap 13 is attached to the upper end of the lid 12 as a separate member, but the cap 13 is not necessarily essential. The buffer container 11 has an opening 11a at the top.
And has a structure in which the buffer solution 14 is stored. The buffer solution container 11 has the same structure as the buffer solution container 1 of the first embodiment, and thus detailed description thereof will be omitted.

On the other hand, the lid 12 has a mounting portion 12 at the bottom.
a. The inner wall of the mounting portion 12a has the opening 1
The outer peripheral wall 11b of the portion forming 1a is sized to be press-fitted. Therefore, when the lid 12 is attached to the outer peripheral wall 11b by the attaching portion 12a, both are fixed in a liquid-tight manner. In addition, the outer peripheral wall 11b,
The both may be fixed in a liquid-tight manner by forming a screw on the inner peripheral wall of the mounting portion 12a on the lid 12 side.

A measuring portion 12b having a diameter relatively smaller than that of the mounting portion 12a is formed above the mounting portion 12a. In the measuring part 12b, the lower part, that is, the opening 11a
The separation filter 15 is arranged on the side, and the measurement filter 16 is attached on the upper side. The respective configurations of the separation filter 15 and the measurement filter 16 are the same as in the first embodiment.

Further, an outlet 12c is provided above the measuring section 12b. The cap 13 described above is attached so as to close the outflow port 12c.
On the other hand, below the lid 12, a rod-shaped stool collection tool 12d is fixed inside the attachment portion 12a. That is, inside the attachment part 12a, a conical stool collection device attachment part 12e extending from the lower end of the measurement part 12b to the rod-shaped stool collection device 12d side is formed. The above-mentioned stool collection device 12d is fixed to the tip of the stool collection device attachment portion 12e. In addition, a plurality of through-holes 12f are formed in the stool collection device mounting portion 12e. The through-hole 12f is provided to allow the stool sample solution to flow down to the measurement unit 12b side.

The stool collection tool 12d is made of a substantially rod-shaped synthetic resin, and has a recess 12g formed on the outer peripheral surface near the tip. A stool sample 17 is collected in the recess 12g as shown in FIG. 7 (b).

In use, after collecting the stool sample 17, the lid 12 is attached to the buffer solution container 11. In this case, the mounting portion 12d is liquid-tightly fixed to the outer peripheral wall 11b. Therefore, by shaking the buffer solution container 11 and the lid 12 in the state shown in FIG.
7 is dissolved in the buffer solution 14 to obtain a stool sample solution. Thereafter, as shown in FIG. 8, after removing the cap 13, the buffer solution container 11 is reversed. As a result, the stool sample solution 14A passes through the through hole 12f and passes through the measurement unit 12
It flows down to the b side. Then, the separation filter 15 removes unnecessary solids from the stool sample solution, and the stool sample solution from which the unnecessary solids have been removed is dropped onto the measurement filter 16.

When fecal occult blood is present on the measurement filter 16, the above-described antigen-antibody reaction occurs, and the measurement filter 16 is colored in a predetermined color. On the other hand, in the absence of fecal occult blood, it is not colored. Therefore, by observing the measurement filter 16 with the naked eye, the presence or absence of fecal occult blood can be easily determined. The fecal sample solution that has passed through the measurement filter 16 flows down from the outlet 12c.

As described above, in the second embodiment, the lid 1
After collecting the stool sample with the stool collection device 12d provided integrally with 2, the lid 12 is attached to the buffer solution container 11, the buffer solution container 11 is shaken, and then the buffer solution container 11 is simply reversed. The presence or absence of fecal occult blood can be easily measured.

The cap 13 is provided so that the internal buffer solution does not leak when the fecal sample solution is prepared. Therefore, when the buffer solution container 11 is shaken so as to prevent the internal buffer solution 14 from leaking when a stool sample is prepared as a solution, the cap 13 may not be necessarily provided.

Further, in the above embodiment, the stool collection device 12d is fixed by the conical stool collection device mounting portion 12e, but instead of the conical stool collection device mounting portion 12e, the stool collection device 12d is covered. By providing a plurality of ribs radially so as to extend toward the measurement portion 12b side of the body 12, the lid 12
Alternatively, the stool collection device 12d may be integrated. In that case,
The stool sample solution passes through the space between the plurality of ribs and the measurement unit 1
It will flow down to the 2b side.

[Brief description of drawings]

1A and 1B are a cross-sectional view and a perspective view showing a stool collection device, showing a state in which the stool collection device prepared in Example 1 is inserted into a buffer solution container.

FIG. 2 is a perspective view for explaining a step of dissolving a stool sample in a state where the stool collection device is immersed in a buffer solution container.

FIG. 3 is a cross-sectional view showing a state in which a measurement lid is attached to a buffer solution container 1 in Example 1.

FIG. 4 is an enlarged cross-sectional view for explaining a measurement lid.

5 (a) and 5 (b) are perspective views for explaining a step of flowing a stool sample solution to a measurement filter side and a partially enlarged cross-sectional view showing a state in which the sample solution is dropped on the measurement filter. .

FIG. 6 is a partially cutaway sectional view schematically showing a state in which an immune complex is formed in the measurement filter.

7A and 7B are a cross-sectional view and a perspective view showing a lid of the fecal occult blood measurement apparatus of Example 2.

FIG. 8 is a perspective view for explaining a process of performing measurement in the fecal occult blood measurement apparatus of Example 2.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Buffer container 2 ... Stool collection instrument 2a ... Stool collection instrument main body 2b ... Lid 3 ... Stool sample 4 ... Buffer 1a ... Opening 5 ... Measurement lid 6 ... Separation filter 7 ... Measurement filter 5b ... Outlet 4A ... stool sample solution 11 ... buffer solution container 12a ... opening 12 ... lid 12c ... outlet 12d ... stool collection device 14 ... buffer solution 15 ... separation filter 16 ... measurement filter 17 ... stool sample

Claims (2)

[Claims] 1. A device for measuring the presence or absence of fecal occult blood, comprising a buffer solution container containing a buffer solution for dissolving a stool sample and having an opening, and the buffer solution container having the opening. A stool collection instrument for inserting a stool sample into a buffer solution by inserting it from above, and a measurement lid body removably and liquid-tightly attached to the opening of the buffer solution container, Is provided with an outlet for letting out a stool sample solution in which the stool sample is dissolved, and between the side attached to the opening of the buffer solution container and the outlet, A fecal occult blood measuring apparatus, characterized in that a separation filter for removing solids and a measurement filter on which an anti-human hemoglobin antibody is immobilized and impregnated with colored latex are arranged in this order. 2. A device for measuring the presence or absence of fecal occult blood, which contains a buffer solution for dissolving a stool sample and has an opening, and an opening of the buffer solution container. A lid body that is liquid-tightly and removably attached, and a rod-shaped fecal collecting device that is provided so as to extend into the buffer solution when attached to the buffer solution container is attached to the lid body. The stool sample is provided with an outlet for letting out the buffer solution in which the stool sample is dissolved, and the lid body is provided between the side attached to the opening of the buffer solution container and the outlet to dissolve the stool sample. A fecal occult blood measuring apparatus, wherein a separation filter for removing solids in a liquid and a measurement filter having an anti-human hemoglobin antibody fixed and impregnated with colored latex are arranged in this order.