JPH07277996A - Method for soothing inflammation of mastitis in ungulata and antiinflammatory solution - Google Patents

Abstract

PURPOSE:To wet the sites infected with bacteria in the udders of Ungulata animal and eradicate the pyogenic bacteria to soothe the inflammation by injecting oxidation potential water from nipples through the ducts into the glands in a prescribed amount. CONSTITUTION:The udder of Ungulata animal which has already caused mastitis and has stiffness therein is injected with oxidation potential water of 2.6pH and 1,080mV ORP from the corresponding nipple in an amount of 500-600ml twice a day in the morning and evening for 20 to 30 minutes. Then, the injected solution is squeezed out as in milking and then the new oxidation potential solution of 2.6pH and 1080mV ORP is injected into the udder again. The same operations are repeated for two days to loosen the udder stiffness and after three days, there is found no anomaly from bacteriological point of view and the number of cell in the milk was restored to the normal level.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-inflammatory solution for suppressing mastitis such as udder of ungulates, especially cows.

[0002]

2. Description of the Related Art Due to the environment surrounding livestock and the handling of livestock, livestock may be infected with spontaneous infections caused by indigenous bacteria or Pseudomonas aeruginosa. For example, when you are lying down on a dairy cow,
There is a risk that the teats and breasts will come into contact with bacteria-contaminated grass and feed, and during milking, if the teats and breast pumps are not clean, Pseudomonas aeruginosa will infect the breasts from the teats. Inadvertently touching the teat of an uninfected dairy cow with fibers or the like that wipes the teat infected with mastitis will cause infection and increase mastitis. When mastitis occurs in a dairy cow, symptoms such as a lump in the udder occur and a fever occurs, which not only weakens the dairy cow and reduces the milk output,
If the number of cells exceeds 300,000, milk becomes unsuitable for drinks.
If the inflammation further expands, it becomes unusable as a dairy cow, and the cow must be abandoned, resulting in economic loss. Also,
When the recovery of the inflammation is delayed, milking cannot be performed for a long time, which is an immeasurable impact on dairy farming.

In order to prevent mastitis, in order to clean the teats and breasts during milking, they are washed with hot water or water, and then wiped with a paper or cloth moistened with a sterilizing agent. . For example, JP-A-3-136649 and JP-A-3-13649.
In No. 136650, it is recommended to prepare various hygroscopic fibers impregnated with and carry antibacterial metal molecules such as silver and copper and polyacrylonitrile fiber containing iodine as a polyacrylonitrile-iodine complex and wipe it off with it. ing.

When mastitis occurs, a method of injecting an antibiotic such as penicillin to kill Pseudomonas aeruginosa is adopted. Although this method is effective, the infused antibiotics may continue to be present in the breast and mix with and contaminate the milk expressed, contaminating milk and drinking milk immediately after the mastitis has subsided. This is extremely dangerous, and even if the inflammation heals, the milk collected must be discarded for a while.

In the method of injecting antibiotics such as penicillin to kill Pseudomonas aeruginosa, the resistance of bacteria increases with the use of antibiotics, and the injection amount of antibiotics must be increased for a while. In addition, various types of additives may be administered to livestock by being mixed with feed. Depending on the type of additive administered to each livestock, a unique resistant bacterium may have been created, and antibiotics such as penicillin described above may not always be heard continuously. There must be.

An electrolyte is added to raw water such as tap water in a fixed ratio and introduced into a closed electrolytic cell, and a direct current is applied between the negative and positive electrodes separated via an ion-permeable diaphragm in the electrolytic cell. , Electrolysis of water and ion permeation, producing cathode water on the cathode side, producing anode water on the anode side, draining the cathode water, and using the anode water as sterilizing water. There is.

Oxidizing potential water is effective as sterilizing water. Moreover, since a sterilizing agent such as an organic sterilizing solution is not added to this oxidizing potential water, it does not contain harmful substances and does not cause irritation or toxicity to living tissues. Further, since the oxidation-reduction potential (hereinafter referred to as ORP) decreases with time, it is useful as sterilizing water that has no residual property and is reliable.

[0008]

SUMMARY OF THE INVENTION Therefore, the present invention is intended to inject the oxidizing potential water obtained from the oxidizing potential water generator into the breasts of livestock to exert a flame-retarding action as an anti-inflammatory liquid harmless to livestock. is there. Further, the present invention intends to prevent the occurrence and early treatment of mastitis and prevent the infection of the epidermis by bacteria by using the oxidative potential water as a liquid for washing the breast of livestock to wash and sterilize the epidermis of the udder and nipple.

[0009]

In view of the above object, the present invention provides a cathode in which an electrolyte added from an electrolyte supply means placed immediately before an electrolytic cell is introduced and divided by an ion permeable diaphragm and a negative electrode is inserted. Anode generated by a device that electrolyzes the raw water flowing into the electrolytic cell by applying a DC voltage between the positive and negative electrodes of the chamber and the positive and negative electrode in which the positive electrode is inserted, and by varying the applied voltage to vary the electrolytic strength of the electrolytic cell. Mastitis is extinguished as an anti-inflammatory solution by injecting water, that is, oxidation potential water, into the ungulate udder.

The injection into the breast is performed by injecting from the nipple with an infusion tube, reversely osmosis myoepithelial cells from the milk duct, and moisten the affected area of bacterial infection against lactation against lactation.

The anti-inflammatory solution has a pH of 2.5 to 3.0 and ORP.
It is 900-1100 mv.

The anti-inflammatory solution has a pH of 2.5 to 3.0 and ORP.
The gist is to contain 0 to 1% of salt at 900 to 1100 mv.

[0013]

[Function] Oxidizing potential water has the effect of killing viruses, bacteria, fungi and the like, and has the property of penetrating and dissolving in water-soluble substances such as blood, body fluids and pus. By injecting a predetermined amount of oxidative potential water from the nipple through the mammary duct of the breast, reverse osmosis of myoepithelial cells occurs, moisturizes the affected area against bacterial lactation against lactation, and kills Pseudomonas aeruginosa. It extinguishes the breast.

Various kinds of oxidation potential water can be obtained depending on the amount of current to the raw water per unit flow rate, the amount of electrolyte added per unit flow rate, etc.
When 3.0 and ORP900-1100mv are used, the target anti-inflammatory solution effective for sterilization can be obtained. Further, if desired, about 0 to 1% of salt is added to the anti-inflammatory solution to obtain a physiological saline-type anti-inflammatory solution, which does not change the virucidal and bactericidal effects and is effective for reverse osmosis of myoepithelial cells.

The anti-inflammatory solution produced as described above has a bacterial deterrent and bactericidal effect against viruses, bacteria and fungi. For example, it has an effect against bacteria and fungi shown in the table below. That is, the indicated bacteria were cultivated in a broth culture solution, and 0.1 ml each of the bacterium solution of each husband and 0.9 ml of the washing solution were mixed to bring the concentration of the anti-inflammatory solution to 90%, and the mixture remained after being treated at room temperature for 3 minutes. The number of bacteria was examined by colony formation. The results are shown in Table 1.
It is confirmed that all the bacteria were completely inactivated, as shown in.

[Table 1] Antiseptic solution bactericidal effect Bacteria, fungus Nominal number of bacteria / ml Untreated 3 minutes treated Staphylococcus aureus 10^8.3 <10⁰ Bacteria E. coli 10^9.0 <10⁰  Bacillus subtilis 10^7.4 <10⁰ Fungus candy da 10^7.3 <10⁰

Further, 1.0% sodium chloride was added to the anti-inflammatory solution, and the bacterial inhibition and bactericidal effects against bacteria and fungi were examined.
That is, the indicated bacteria were cultivated in a broth culture solution, and each of the bacteria solutions of the husband and wife was 0.
1 ml and 0.9 ml of a washing solution containing 1.0% sodium chloride were mixed to adjust the concentration of the anti-inflammatory solution to 90%, and after treatment at room temperature for 2 minutes, the number of remaining bacteria was examined by colony formation. As shown in Table 2, the results confirm that all the bacteria were completely inactivated.

[Table 2] Bactericidal effect of anti-inflammatory solution containing salt Bacteria, fungus Nominal number of bacteria / ml Untreated 2-minute treatment Budococcus 10 ^8.4 <10 ⁰ bacteria Streptococcus 10 ^8.2 <10 ⁰ Escherichia coli 10 ^9.0 <10 ⁰ Pseudomonas aeruginosa Fungus 10 ^8.8 <10 ⁰ Bacillus subtilis 10 ^6.0 <10 ⁰ Fungus Candida 10 ^8.2 <10 ⁰

[0017]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 is an explanatory view of an anti-inflammatory solution producing apparatus according to the present invention. In the figure, the city water electrolysis tank 4 whose flow rate is controlled by a valve body 1 for controlling the flow rate of city water flowing into the electrolysis cell 4
From the electrolyte supply means 3 placed immediately before, NaCl, KCl
A predetermined amount of electrolyte such as is added to the electrolytic bath 4. Electrolyzer 4
Is divided by the ion-permeable diaphragm 41, and is configured to apply a DC voltage between the cathode and cathode electrodes 42 and 43 having the cathode and anode electrodes inserted therein and the anode and anode electrodes 43, respectively. Applied voltage varying means for varying the electrolytic strength is provided. A filtration device may be placed between the valve body 1 and the electrolyte supply means 3. The filtration device is composed of, for example, a member suitable for blocking pollutants such as hollow fibers or particles having a certain particle size or bacteria, activated carbon, and a combination thereof. To be removed. The water discharged from the anode chamber 43 of the electrolytic cell, that is, the oxidation potential water, has a predetermined ORP, pH and the like confirmed by the sensor means 5 such as a redox potential meter and a hydrogen ion concentration meter. It flows into the tank 6 via the stop valve 11.

Whether or not the amount of water supplied to the electrolytic cell, the amount of electrolyte supplied to the electrolytic solution supplying means 3, the voltage applied to the negative and positive electrodes of the electrolytic cell, etc. are appropriate values is a sensor for measuring the oxidation potential water discharged from the anode chamber. It can be known from the ORP and pH values of the means 5. Predetermined value of oxidation potential water is ORP 900 mv or more, p
In the case of H3.0 or less, water is supplied to the mixing tank 6. Any other value is drained from the three-way valve 12. In addition, the cathode water discharged from the cathode chamber is also drained. Oxidation potential water discharged in a preferable state is usually ORP950 to 1090 m.
Those having a pH of about 2.8 or less and a pH of 2.8 or less and stored in a non-light-transmitting bottle are relatively stable and function as the anti-inflammatory solution of the present invention. In addition, if desired, the mixing tank 6 may have 0 to 1
It is also possible to add about% sodium chloride to form a physiological saline-containing anti-inflammatory solution.

In FIG. 1, reference numeral 10 is a control means which controls the amount of water supplied to the electrolytic cell, the amount of electrolyte supplied by the electrolyte supply means, the electrolytic applied voltage, etc. based on the data from the sensor means 5, ORP,
The pH is automatically controlled so that it falls within the standard range. That is, if a desired value is input to the ROM that constitutes a part of the control means, the comparison circuit operates based on the data from the sensor means 5 and the amount of water supplied to the electrolytic cell and the electrolyte supply of the electrolyte supply means are within a desired range. The amount, the voltage applied to the electrolysis, and the like can be controlled.

The sensor means 8 is a means for measuring the ORP. Based on the information from this means, when the oxidation potential water in the tank 6 does not indicate the above-mentioned ORP, the drain 9 is used.
Can be drained through. Oxidation potential water decreases ORP with time. Therefore, when the anti-inflammatory liquid that has already been used remains inside the tank 6, there is a possibility that the sterilizing ability thereof may be reduced. This can also be automatically eliminated by the control means 10 as in the above.

An example of injecting the above anti-inflammatory solution into a dairy cow having mastitis will be described. From a corresponding teat that had mastitis symptoms in the udder of a dairy cow, which was close to abandoned cows that had already developed mastitis and had a lump in the udder and had a reduced appetite for feed, pH 2.
6. ORP 1080mv anti-inflammatory solution 500 times twice in the morning and evening
After injecting ~ 600 ml with an injector and leaving it for 20 to 30 minutes, the anti-inflammatory solution is squeezed out by the same operation as the milking, and once the anti-inflammatory solution in the breast is squeezed out, pH is 2.6 again, ORP108.
0 mV anti-inflammatory solution was injected with a syringe. I did the same thing for two days. First, the lump was removed, and after three days, there was no abnormality in the bacteriological test, and the number of milk cells returned to the normal value. The injection amount is not limited to the above-mentioned values, and may be injected until the milk line is saturated. Further, the injection / squeeze of the anti-inflammatory solution is not necessarily limited to one time, and it is preferable to repeat it until 900 mv is reached after confirming the ORP of the expressed anti-inflammatory solution.

1000 ml each of an anti-inflammatory solution containing 0.9% of salt that had been produced for 1 hour after injection was injected into ¼ of the udder of a cow with acute mastitis and fever, three times each in the morning, evening and morning. Injected with a container. The next morning, he became sick and had a strong appetite, and the dairy cows were free from the discomfort observed during acute mastitis and were ready for milking. The milk level returned to the normal level as measured by the PL tester. No anti-inflammatory solution was found in the milk collected in this state,
No traces of organic additives, antibiotics, etc. were found in the collected milk. An anti-inflammatory solution containing salt is understood to be equivalent to bactericidal physiological saline, reversely osmosis myoepithelial cells from the mammary duct, moisten the bacterial infected area against lactation against lactation, and produce good results. Is what you get.

A 200 ml syringe was used to inject an anti-inflammatory solution containing 0.3% salt from the teat of a goat suffering from acute mastitis.
The next morning I had a fever. Milking could be done normally.

FIG. 2 is an injection execution diagram. For example, the anti-inflammatory solution in the container 16 is injected into the teat of a dairy cow through the thin tube 17 and the injection tube
Inject from 8. As the injector, for example, a gravity drip type container such as a drip injector is used, a thin tube is inserted into the teat, and the position of the container 16 is moved up and down as shown by an arrow so that a predetermined amount of the milk can be applied to the breast immediately after milking. Although the quenching liquid is injected, the container 16 may be of a pressure type. Further, the diameter and the length of the injection tube 18 are appropriately selected depending on the type of animal.

[0025] Oxidizing potential water can be used as a lavage fluid for livestock to wash and sterilize the udder and nipple epidermis to prevent and treat mastitis at an early stage and to prevent bacterial epidermal infection. These are merely examples, and various modifications can be made within the scope of the present invention other than the embodiments described above.

[0026]

INDUSTRIAL APPLICABILITY The anti-inflammatory solution has the effect of killing viruses, bacteria, fungi and the like, and has the property of penetrating and dissolving in water-soluble substances such as blood, body fluid and pus. Kill the bacteria that cause mastitis by reversely osmoticizing myoepithelial cells from the mammary duct of the breast through the nipple and injecting a predetermined amount into the affected area of the mammary vesicle against lactation. can do.

For injection into the breast, with an injection tube from the nipple,
It does not cause any damage to the cow because it can be done in a simple way by reverse osmosis of the myoepithelial cells from the milk duct to moisturize the affected area against lactation into the mammary tract.

The determination of whether or not the anti-inflammatory solution remains in the breast can be easily known from the fact that the milk of milking changes from colorless to milky white, and therefore the time for re-injection of the anti-inflammatory solution can be accurately determined.

Since the anti-inflammatory solution does not contain residual substances such as bactericidal drugs and antibiotics, no harmful substances are contained in the cured milk. That is, the anti-inflammatory solution has a low ORP with time, has no residual property, and can be supplied at a low cost.

[Brief description of drawings]

FIG. 1 is an explanatory diagram of an anti-inflammatory liquid generator according to the present invention.

FIG. 2 is an injection procedure diagram.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Valve body 3 Electrolyte supply means 4 Electrolyzer 5 Sensor means 8 Sensor means 9 Drain 10 Control means 11 Check valve 12 Three-way valve 16 Container 17 Capillary tube 18 Injection pipe

Claims (4)

[Claims] 1. A positive and negative electrode chamber in which an electrolyte added from an electrolyte supply means placed immediately before the electrolytic cell is introduced and divided by an ion-permeable diaphragm, and a cathode chamber in which a negative electrode is inserted and an anode chamber in which a positive electrode is inserted are formed. Applying a DC voltage between the electrodes and providing means for varying the applied voltage to vary the electrolytic strength of the electrolyzer, and injecting oxidative potential water generated by the device that electrolyzes the raw water that has flowed into the electrolyzer into the breasts of ungulates A method for suppressing ungulate mastitis, which comprises suppressing mastitis as an anti-inflammatory solution according to. 2. The injection into the breast is performed by injecting from the nipple with an injection tube, reversely osmosis myoepithelial cells from the milk duct, and moisten a bacterial infection affected area against lactation against lactation. The method for quenching ungulate mastitis according to claim 1. 3. The anti-inflammatory solution has a pH of 2.5 to 3.0 and OR.
The anti-inflammatory solution for ungulate mastitis according to claim 1, which has a P900 to 1100 mv. 4. The anti-inflammatory solution has a pH of 2.5 to 3.0 and OR.
The anti-inflammatory solution for ungulate mastitis according to claim 1, which contains 0 to 1% of salt at P900 to 1100 mv.