JPH07120681A - Illuminating device of microscope - Google Patents
Illuminating device of microscopeInfo
- Publication number
- JPH07120681A JPH07120681A JP26932193A JP26932193A JPH07120681A JP H07120681 A JPH07120681 A JP H07120681A JP 26932193 A JP26932193 A JP 26932193A JP 26932193 A JP26932193 A JP 26932193A JP H07120681 A JPH07120681 A JP H07120681A
- Authority
- JP
- Japan
- Prior art keywords
- lens
- image
- illumination
- light source
- condenser lens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、顕微鏡照明装置、特
に、一次元の撮像素子による顕微鏡像の撮像に適用しう
る顕微鏡照明装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microscope illuminating device, and more particularly to a microscope illuminating device applicable to the taking of a microscope image by a one-dimensional image pickup device.
【0002】[0002]
【従来の技術】従来の顕微鏡照明装置について図面を参
照して詳細に説明する。2. Description of the Related Art A conventional microscope illumination device will be described in detail with reference to the drawings.
【0003】従来の技術としては、例えば、「光工学ハ
ンドブック」634頁から635頁に記載の、一般に”
ケーラー照明”と呼ばれる顕微鏡照明装置がある。As a conventional technique, for example, a general technique described in "Optical Engineering Handbook", pages 634 to 635, is generally used.
There is a microscope illuminator called "Kohler illuminator".
【0004】図3は従来の顕微鏡照明装置の一例を示す
構成図である。FIG. 3 is a block diagram showing an example of a conventional microscope illuminating device.
【0005】この従来例の顕微鏡照明装置は、照明光を
発する光源1と、照明光軸中にあって照明光を集光する
集光レンズであるコレクタレンズ2と、コレクタレンズ
2が光源1の像を結像する位置に配置した開口絞り3
と、開口絞り3の位置に前側焦点位置を一致させて配置
したコンデンサレンズ4とを含んで構成される。In this conventional microscope illuminating device, a light source 1 which emits illumination light, a collector lens 2 which is a condenser lens for converging the illumination light in the illumination light axis, and the collector lens 2 are the light sources 1. Aperture stop 3 placed at the position where an image is formed
And a condenser lens 4 arranged such that the front focus position is aligned with the position of the aperture stop 3.
【0006】次に、従来例の顕微鏡照明装置の動作につ
いて図3を参照して説明する。Next, the operation of the conventional microscope illuminating device will be described with reference to FIG.
【0007】集光レンズであるコレクタレンズ2は照明
光軸中に配置し、開口絞り3はコレクタレンズ2が光源
1の像を結像する位置に配置する。また、コンデンサレ
ンズ4は対物レンズ7との合成の前側焦点位置を開口絞
り3の位置に一致させて配置する。ハーフミラー6はコ
ンデンサレンズ4と対物レンズ7の間に配置し、照明光
を被撮像物8側に折り曲げる。結像レンズ10は対物レ
ンズ7と共に顕微鏡結像光学系を構成し、被撮像物8の
拡大像を一次元の撮像素子であるラインセンサ11上に
結像する。A collector lens 2 which is a condenser lens is arranged in the illumination optical axis, and an aperture stop 3 is arranged at a position where the collector lens 2 forms an image of the light source 1. Further, the condenser lens 4 is arranged so that the front focus position of the combination with the objective lens 7 coincides with the position of the aperture stop 3. The half mirror 6 is arranged between the condenser lens 4 and the objective lens 7, and bends the illumination light toward the object 8 side. The image forming lens 10 constitutes a microscope image forming optical system together with the objective lens 7, and forms an enlarged image of the object 8 on the line sensor 11 which is a one-dimensional image pickup device.
【0008】まず、コレクタレンズ2は光源1の像を開
口絞り3の位置に結像する。次に、照明光はコンデンサ
レンズ4、ハーフミラー7、対物レンズ7を通して被撮
像物8上に照射されるが、このとき、開口絞り3はコン
デンサレンズ4と対物レンズ7の合成の前側焦点位置に
あるため光源1の像は無限遠にでき、被撮像物8上には
照明むらの無い円形の照明領域9が形成される。First, the collector lens 2 forms an image of the light source 1 at the position of the aperture stop 3. Next, the illumination light is irradiated onto the object 8 through the condenser lens 4, the half mirror 7, and the objective lens 7. At this time, the aperture stop 3 is located at the front focus position of the combination of the condenser lens 4 and the objective lens 7. Therefore, the image of the light source 1 can be made infinite, and a circular illumination area 9 having no illumination unevenness is formed on the object 8 to be imaged.
【0009】[0009]
【発明が解決しようとする課題】上述した従来の顕微鏡
照明装置は、一次元の撮像素子で撮像する場合に、照明
領域が円形なので撮像領域以外の部分も照明しているた
め、照明効率が低いという欠点があった。The above-described conventional microscope illuminating device has low illumination efficiency when the image is picked up by a one-dimensional image pickup element because the illumination area is circular and illuminates a portion other than the image pickup area. There was a drawback.
【0010】[0010]
【課題を解決するための手段】本発明の顕微鏡照明装置
は、照明光を発する光源と、照明光軸中にあって照明光
を集光する集光レンズと、集光レンズが光源の像を結像
する位置に配置した開口絞りと、開口絞りの位置に前側
焦点位置を一致させて配置したコンデンサレンズと、コ
ンデンサレンズとの組合せで開口絞りの位置に結像した
光源の像を被撮像物上に結像する位置に配置した円筒状
レンズとを含むことを特徴としている。A microscope illuminator according to the present invention includes a light source for emitting illumination light, a condenser lens for converging the illumination light in the illumination optical axis, and the condenser lens for forming an image of the light source. The image of the light source formed at the position of the aperture stop by the combination of the aperture stop placed at the position where the image is formed, the condenser lens having the front focus position aligned with the position of the aperture stop, and the condenser lens is taken. It is characterized in that it includes a cylindrical lens arranged at a position where an image is formed above.
【0011】[0011]
【実施例】次に、本発明の実施例について図面を参照し
て説明する。Embodiments of the present invention will now be described with reference to the drawings.
【0012】図1は本発明による顕微鏡照明装置の一実
施例を示す構成図である。FIG. 1 is a block diagram showing an embodiment of a microscope illuminating device according to the present invention.
【0013】この実施例の顕微鏡照明装置は、照明光を
発する光源1と、照明光軸中にあって照明光を集光する
集光レンズであるコレクタレンズ2と、コレクタレンズ
2が光源1の像を結像する位置に配置した開口絞り3
と、開口絞り3の位置にその合成の前側焦点位置を一致
させて配置したコンデンサレンズ4及び対物レンズ7
と、コンデンサレンズ4と対物レンズ7との間に配置し
コンデンサレンズ4及び対物レンズ7との組合せで開口
絞り3の位置に結像した光源1の像を被撮像物8上に結
像するシリンドリカル(円筒状)レンズ5と、シリンド
リカルレンズ5と対物レンズ7との間に配置し照明光を
被撮像物8側に折り曲げるハーフミラー6と、顕微鏡結
像光学系を構成する対物レンズ7及び結像レンズ10
と、結像レンズ10の結像位置に配置した一次元の撮像
素子であるラインセンサ11とを含んで構成される。In the microscope illuminator of this embodiment, a light source 1 for emitting illumination light, a collector lens 2 which is a condenser lens for converging the illumination light in the illumination light axis, and the collector lens 2 are the light sources 1 Aperture stop 3 placed at the position where an image is formed
And the condenser lens 4 and the objective lens 7 arranged so that their combined front focal positions coincide with the position of the aperture stop 3.
And a cylindrical lens which is disposed between the condenser lens 4 and the objective lens 7 and forms an image of the light source 1 formed on the position of the aperture stop 3 by the combination of the condenser lens 4 and the objective lens 7 on the object 8. A (cylindrical) lens 5, a half mirror 6 arranged between the cylindrical lens 5 and the objective lens 7 to bend the illumination light toward the object 8 to be imaged, an objective lens 7 forming an image forming optical system of the microscope, and an image formation. Lens 10
And a line sensor 11 which is a one-dimensional image sensor arranged at the image forming position of the image forming lens 10.
【0014】次に、本実施例の顕微鏡照明装置の動作に
ついて図1、図2を参照して説明する。Next, the operation of the microscope illuminating device of this embodiment will be described with reference to FIGS.
【0015】図2は本発明の原理を示す光学配置図、図
2(a)と図2(b)は光学配置が90度異なる方向の
図である。FIG. 2 is an optical layout diagram showing the principle of the present invention, and FIGS. 2 (a) and 2 (b) are views showing directions in which the optical layouts differ by 90 degrees.
【0016】図2(a)はシリンドリカルレンズ5がレ
ンズとして作用する面であり、また、簡単のため、ハー
フミラー6と撮像側の光学系は省略しており、各レンズ
は理想レンズとして主平面の位置のみを示してある。こ
のうち、合成主平面(a)12はコンデンサレンズ4と
シリンドリカルレンズ5と対物レンズ7との組合せの主
平面であり、図2(b)の面ではシリンドリカルレンズ
5はレンズとして作用しないため、合成主平面(b)1
3はコンデンサレンズ4と対物レンズ7との組合せの主
平面である。FIG. 2A shows a surface on which the cylindrical lens 5 acts as a lens. Further, for simplicity, the half mirror 6 and the optical system on the image pickup side are omitted, and each lens is an ideal lens as a main plane. Only the position of is shown. Of these, the composite principal plane (a) 12 is the principal plane of the combination of the condenser lens 4, the cylindrical lens 5, and the objective lens 7. Since the cylindrical lens 5 does not act as a lens in the plane of FIG. Main plane (b) 1
Reference numeral 3 is a principal plane of a combination of the condenser lens 4 and the objective lens 7.
【0017】まず、コレクタレンズ2が光源1の像を開
口絞り3の位置に結像する。次に、照明光はコンデンサ
レンズ4、シリンドリカルレンズ5、ハーフミラー6、
対物レンズ7を通して被撮像物8上に照射されるが、こ
のとき、図2(a)に示す平面上では、シリンドリカル
レンズ5がレンズとして作用し、合成主平面(a)12
により開口絞り3の位置に結像した光源1の像を被撮像
物8上に結像する。ここで、合成主平面(a)12につ
いての物距離をS、像距離をS’とすると、このときの
結像倍率mは、 m = S’/S であり、 m << 1 となるようにシリンドリカルレンズ5の配置と焦点距離
を決定する。また、図2(b)に示す平面上では、シリ
ンドリカルレンズ5はレンズとして作用せず、開口絞り
3は合成主平面(b)13の前側焦点位置にあるため、
合成主平面(b)13による光源1の像は無限遠に結像
される。First, the collector lens 2 forms an image of the light source 1 at the position of the aperture stop 3. Next, the illumination light is a condenser lens 4, a cylindrical lens 5, a half mirror 6,
The object 8 is irradiated with the light through the objective lens 7. At this time, the cylindrical lens 5 acts as a lens on the plane shown in FIG.
Thus, the image of the light source 1 formed at the position of the aperture stop 3 is formed on the object 8 to be imaged. Here, assuming that the object distance and the image distance are S and S ′ with respect to the synthetic principal plane (a) 12, the imaging magnification m at this time is m = S ′ / S, and m << 1 Then, the arrangement of the cylindrical lens 5 and the focal length are determined. Further, on the plane shown in FIG. 2B, since the cylindrical lens 5 does not function as a lens and the aperture stop 3 is at the front focal point of the synthetic main plane (b) 13,
The image of the light source 1 by the synthetic principal plane (b) 13 is formed at infinity.
【0018】これにより、被撮像物8上には、長軸方向
に照明むらの無い細長い楕円状の照明領域9が形成さ
れ、この照明領域9内の被撮像物8の像を、対物レンズ
7、結像レンズ10によりラインセンサ11に拡大結像
して撮像を行う。As a result, an elongated elliptical illumination area 9 having no illumination unevenness in the major axis direction is formed on the object 8 to be imaged, and the image of the object 8 in the illumination area 9 is formed by the objective lens 7. A magnified image is formed on the line sensor 11 by the imaging lens 10 and an image is taken.
【0019】尚、開口絞り3の径を可変にすることによ
り、明るさ及び像コントラストを変化させることができ
る。The brightness and the image contrast can be changed by making the diameter of the aperture stop 3 variable.
【0020】[0020]
【発明の効果】以上説明したように、本発明の顕微鏡照
明装置は、コンデンサレンズとともに円筒状レンズを使
用し、被撮像物上に長軸方向に照明むらの無い細長い楕
円状の照明領域を形成することにより、一次元の撮像素
子で撮像する場合にその撮像領域のみに照明光を集光で
き、照明効率を高くできるという効果がある。As described above, the microscope illuminator of the present invention uses a cylindrical lens together with a condenser lens to form a long and narrow elliptical illumination area on the object to be imaged with no illumination unevenness in the major axis direction. By doing so, when the image is captured by the one-dimensional image sensor, the illumination light can be condensed only in the image capturing area, and the illumination efficiency can be improved.
【図1】本発明による顕微鏡照明装置の一実施例を示す
構成図である。FIG. 1 is a configuration diagram showing an embodiment of a microscope illumination device according to the present invention.
【図2】本発明の原理を示す光学配置図である。図2
(a)と図2(b)は光学配置が90度異なる方向の図
である。FIG. 2 is an optical layout diagram showing the principle of the present invention. Figure 2
FIG. 2A and FIG. 2B are diagrams in which the optical arrangement is different by 90 degrees.
【図3】従来の顕微鏡照明装置の一例を示す構成図であ
る。FIG. 3 is a configuration diagram showing an example of a conventional microscope illumination device.
1 光源 2 コレクタレンズ 3 開口絞り 4 コンデンサレンズ 5 シリンドリカルレンズ 6 ハーフミラー 7 対物レンズ 8 被撮像物 9 照明領域 10 結像レンズ 11 ラインセンサ 12 合成主平面(a) 13 合成主平面(b) 1 Light Source 2 Collector Lens 3 Aperture Stop 4 Condenser Lens 5 Cylindrical Lens 6 Half Mirror 7 Objective Lens 8 Image Object 9 Illumination Area 10 Imaging Lens 11 Line Sensor 12 Synthetic Main Plane (a) 13 Synthetic Main Plane (b)
Claims (2)
って照明光を集光する集光レンズと、前記集光レンズが
前記光源の像を結像する位置に配置した開口絞りと、前
記開口絞りの位置に前側焦点位置を一致させて配置した
コンデンサレンズと、前記コンデンサレンズとの組合せ
で前記開口絞りの位置に結像した前記光源の像を被撮像
物上に結像する位置に配置した円筒状レンズとを含むこ
とを特徴とする顕微鏡照明装置。1. A light source for emitting illumination light, a condenser lens for converging the illumination light in the illumination optical axis, and an aperture stop arranged at a position where the condenser lens forms an image of the light source. A position at which an image of the light source formed at the position of the aperture stop is formed on the object to be imaged by a combination of a condenser lens arranged such that the front focal position is aligned with the position of the aperture stop and the condenser lens. A microscope illuminating device, comprising:
徴とする請求項1記載の顕微鏡照明装置。2. The microscope illumination device according to claim 1, wherein a diameter of the aperture stop is variable.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5269321A JP2773607B2 (en) | 1993-10-28 | 1993-10-28 | Microscope lighting device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5269321A JP2773607B2 (en) | 1993-10-28 | 1993-10-28 | Microscope lighting device |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH07120681A true JPH07120681A (en) | 1995-05-12 |
JP2773607B2 JP2773607B2 (en) | 1998-07-09 |
Family
ID=17470729
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5269321A Expired - Lifetime JP2773607B2 (en) | 1993-10-28 | 1993-10-28 | Microscope lighting device |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2773607B2 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002006226A (en) * | 2000-06-19 | 2002-01-09 | Sony Corp | Inspecting device |
US7248404B2 (en) | 2004-05-17 | 2007-07-24 | Olympus Corporation | Microscope |
JP2008249804A (en) * | 2007-03-29 | 2008-10-16 | Fujifilm Corp | Confocal fluorescence microscope |
US7480095B2 (en) | 2004-05-17 | 2009-01-20 | Olympus Corporation | Microscope |
JP2022511676A (en) * | 2018-11-01 | 2022-02-01 | 華中科技大学蘇州脳空間信息研究院 | High Throughput Optical Tomography 3D Imaging System |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS53135653A (en) * | 1977-05-01 | 1978-11-27 | Canon Inc | Photoelectric detecting optical device |
-
1993
- 1993-10-28 JP JP5269321A patent/JP2773607B2/en not_active Expired - Lifetime
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS53135653A (en) * | 1977-05-01 | 1978-11-27 | Canon Inc | Photoelectric detecting optical device |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002006226A (en) * | 2000-06-19 | 2002-01-09 | Sony Corp | Inspecting device |
JP4560900B2 (en) * | 2000-06-19 | 2010-10-13 | ソニー株式会社 | Inspection device |
US7248404B2 (en) | 2004-05-17 | 2007-07-24 | Olympus Corporation | Microscope |
US7480095B2 (en) | 2004-05-17 | 2009-01-20 | Olympus Corporation | Microscope |
JP2008249804A (en) * | 2007-03-29 | 2008-10-16 | Fujifilm Corp | Confocal fluorescence microscope |
JP2022511676A (en) * | 2018-11-01 | 2022-02-01 | 華中科技大学蘇州脳空間信息研究院 | High Throughput Optical Tomography 3D Imaging System |
US11906723B2 (en) | 2018-11-01 | 2024-02-20 | Hust-Suzhou Institute For Brainsmatics | High-throughput optical sectioning three-dimensional imaging system |
Also Published As
Publication number | Publication date |
---|---|
JP2773607B2 (en) | 1998-07-09 |
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