JPH07120392A - Deciding apparatus for base sequence - Google Patents

Deciding apparatus for base sequence

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Publication number
JPH07120392A
JPH07120392A JP5270514A JP27051493A JPH07120392A JP H07120392 A JPH07120392 A JP H07120392A JP 5270514 A JP5270514 A JP 5270514A JP 27051493 A JP27051493 A JP 27051493A JP H07120392 A JPH07120392 A JP H07120392A
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Japan
Prior art keywords
fluorescence
electrophoretic
light
gel
optical system
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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JP5270514A
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Japanese (ja)
Inventor
Junichi Kita
Kenji Takubo
純一 喜多
健二 田窪
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Shimadzu Corp
株式会社島津製作所
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Priority to JP5270514A priority Critical patent/JPH07120392A/en
Publication of JPH07120392A publication Critical patent/JPH07120392A/en
Application status is Pending legal-status Critical

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Abstract

PURPOSE: To exclude the scanning function of an optical system, to eliminate a need for the complicated adjustment of an optical axis and to increase an S/N ratio and a coefficient of light utilization by a method wherein a light source which shines excitation light a fluorescence-labeled DNA fragment and a detector which receives generated fluorescence, are integrated and they and arranged in every electrophoretic lane.
CONSTITUTION: A gel electrophoretic plate 1 is formed in such a way that, e.g. a 6-% polyacrylamide gel 1b in a thickness of 0.35mm is sandwiched between Pyrex glass plates 1a, 1b in a thickness of 0.5mm, and a fluorescence-labeled DNA fragment is injected into a recessed part in a well 2. Consequently, the recessed part in the well 2 having a width of 5 to 10mm is extended in the length direction of the electrophoretic plate 1, and it is used as an electrophoretic lane. Both ends of the electrophoretic plate 1 are inserted into buffer-solution tanks 3, 4 to which one pair of electrodes are attached, and they are connected to a high-voltage power supply. One piece each of a sensor head 7 is arranged in every electrophoretic lane at a mount part 6, excitation light from an LED is cast on the gel 1b, fluorescence from the DNA fragment is received by an avalanche photodiode (APD) 71, and a base arrangement is decided by a CPU.
COPYRIGHT: (C)1995,JPO

Description

【発明の詳細な説明】 DETAILED DESCRIPTION OF THE INVENTION

【0001】 [0001]

【産業上の利用分野】本発明は、DNAなどの塩基配列決定装置であって、更に詳しくは蛍光ラベルをしたDN The present invention relates to a sequencing apparatus, such as a DNA, more particularly to a fluorescent label DN
A断片をゲル電気泳動させ、泳動方向と交わる方向に蛍光の励起・受光光学系を配置し、DNA断片の泳動パターンを検出するものに関する。 The A fragment was gel electrophoresed, the excitation and light receiving optical system of the fluorescence is disposed in a direction crossing the direction of electrophoresis, relates detects the electrophoresis pattern of the DNA fragments.

【0002】 [0002]

【従来の技術】プライマー部又はダイオキシ部に蛍光ラベルを付し、サンガーの方法で調整したDNA断片をゲル電気泳動を行い、得られる展開パターンを解析してD Subjected fluorescent label BACKGROUND ART primer portion or dioxin section, was subjected to gel electrophoresis adjusted DNA fragment Sanger method, by analyzing the development pattern obtained D
NAの塩基配列を決定する塩基配列決定装置が知られている。 Sequencing device for determining the nucleotide sequence of the NA is known.

【0003】代表的な塩基配列決定装置としては、例えば特開昭63−313035号公報に記載にものを挙げることができる。 [0003] Exemplary sequencing apparatus or a thing as described in JP Sho 63-313035. この装置は、図6に示されるものであり、ガラス板に挟まれて図で紙面垂直方向に延びる泳動ゲル104に蛍光ラベルしたサンプルが紙面垂直方向に泳動する。 This apparatus is that shown in Figure 6, sample fluorescence labels gel 104 extending in the direction perpendicular to the paper surface in FIG sandwiched glass plate migrates in the direction perpendicular to the paper surface.

【0004】ステージ239はガイドレール233に案内され、モータ237で駆動される棒ネジ252の回転によって泳動方向と直交する図の上下方向に走査される。 [0004] Stage 239 is guided by the guide rail 233, it is scanned in the vertical direction in the drawing perpendicular to the direction of electrophoresis by the rotation of the rod screw 252 driven by a motor 237. ステージ239には集光レンズ260が設けられ、 A condenser lens 260 is provided on the stage 239,
励起光であるレーザビーム250がミラー251で反射されてレンズ260に入射し、ステージ239上のミラー255で反射されて泳動ゲル104の測定部分を照射する。 The laser beam 250 is excitation light is reflected by the mirror 251 enters the lens 260, it is reflected by the mirror 255 on the stage 239 is irradiated with measurement portion of the gel 104.

【0005】その測定部分から出た蛍光はステージ23 [0005] The fluorescence emitted from the measurement part stage 23
9に設けられた集光レンズ221で集光され、干渉フィルタ223で分光されてレンズ225を通り、光電子増倍管229で検知される。 Is condensed by the condenser lens 221 provided in the 9, passes through a lens 225 is dispersed by the interference filter 223 and is detected by photomultiplier tube 229.

【0006】 [0006]

【発明が解決しようとする課題】しかし、図6の装置では、励起・受光光学系をステージ239に載せ走査しているため、高速で各泳動レーンの信号をサンプリングしようとすれば、ステージ239を高速駆動するための特別な駆動機構を必要とし、装置全体の大型化を招いていた。 [SUMMARY OF THE INVENTION However, in the apparatus of Figure 6, since the scanning place the excitation and light receiving optical system in the stage 239, if an attempt sampled signals of each migration lane at high speed, the stage 239 require special drive mechanism for high-speed driving, we had an increase in size of the entire apparatus.

【0007】また、図6の装置では、泳動ゲル104と走査方向(ネジ252とガイドレール233で決まる)、及び集光レンズ260への励起光の入射方向の三者が全く平行で、かつ泳動ゲル104の位置は走査のどの場所でも全く一致していなければならないが、図7に示すような状態になっている場合がある。 [0007] In the apparatus shown in FIG. 6, (determined by the screw 252 and the guide rail 233) gel 104 to the scanning direction, and the direction of incidence of the excitation light to the condenser lens 260 triadic exactly parallel, and migration position of the gel 104 is must completely match anywhere in the scan, there is a case where in the state shown in FIG. すなわち、走査のある場所Aでは励起光250により照明されたゲル部分は正しく受光光学系の光軸上にあり、強い信号が得られるが、ゲル104が挟まれているガラス板の厚さが変化したり、ゲル104とネジ252の平行度が不十分な場合など、走査の別の場所Bでは図のように励起光2 That is, the location A in the illuminated gel portion by the excitation light 250 a scan is on the optical axis of the right light-receiving optical system, a strong signal is obtained, variable thickness of the glass plates gel 104 is sandwiched or, a gel 104 and the like parallelism if insufficient screws 252, as in another location B Figure the scanning excitation light 2
50により照明されたゲル部分と受光光学系の光軸との間にずれが生じ、信号強度が弱くなり、はなはだしい場合には信号が検出されなくなった。 Deviation between the illuminated gel portion and the optical axis of the light receiving optical system caused by 50, the signal strength is weak, the signal is the extreme case is no longer detected.

【0008】そこで、本発明は、上記に鑑みなされたもので、励起・受光光学系の走査及び複雑な光軸調整が不要で、しかも光利用率が高くS/N比の良くなる塩基配列決定装置を提供することを目的とする。 [0008] The present invention has been made in view of the above, the excitation-scanning and complicated optical axis adjustment of the light receiving optical system is not required, yet may become sequencing of the light utilization factor is high S / N ratio and to provide a device.

【0009】 [0009]

【課題を解決するための手段】本発明は、上記課題を解決するため、蛍光ラベルしたDNA断片を複数のレーンでゲル電気泳動させ、泳動方向と交わる方向に蛍光の励起・受光光学系を配置し、DNA断片の泳動パターンを検出する塩基配列決定装置において、前記励起・受光光学系を一体化して、各泳動レーン毎に配置したことを特徴とする。 Means for Solving the Problems The present invention for solving the above problems, is gel electrophoresis fluorescence labeled DNA fragments by a plurality of lanes, arranged excitation and light receiving optical system of the fluorescence in a direction intersecting the direction of electrophoresis and, in the nucleotide sequence determination system for detecting the electrophoresis pattern of the DNA fragments, by integrating the excitation and light receiving optical system, characterized in that arranged in each migration lane.

【0010】ここで、励起・受光光学系とは、DNA断片の蛍光ラベルに励起光を照射する光源、発した蛍光を受光する検出器を少なくとも備えたもので、光源としては、LED、検出器としてはフォトダイオードを用いるのが小形化の点で特に好ましいが、これに限定されない。 [0010] Here, the excitation and light receiving optical system, which was equipped with at least a detector for receiving a light source, a fluorescent light emitted for irradiating excitation light to the fluorescent label of a DNA fragment, as a light source, LED, detectors as it is particularly preferred in view of miniaturization to use a photodiode, but is not limited thereto. また、フォトダイオードとしては、感度を考慮すると高感度検出が可能なアバランシェ・フォトダイオードを用いるのが好ましい。 As the photodiode, to use the sensitivity of consideration and high sensitivity detection can avalanche photodiode preferred. なお、アバランシェフォト・ダイオードとは、pn接合に逆方向バイアスを十分印加し、空乏層を広げ、空乏層の高電界でそこで発生したキャリアを発生させ、原子との衝突によって新たな電子正孔対を発生させるものである。 Note that the avalanche photodiode, the reverse bias is sufficiently applied to the pn junction, spread the depletion layer to generate carriers generated therein in a high electric field of the depletion layer, a new electron-hole pairs by collisions with atoms it is intended to generate.

【0011】励起・受光光学系には、必要に応じ光源からの励起光をDNA断片の蛍光ラベルにスポット照射するための集光レンズ、発した蛍光を検出器に集めるための集光レンズ等も含まれる。 [0011] excitation and the light receiving optical system, a condenser lens for fluorescent labels spot irradiation of DNA fragments excitation light from optionally light sources, even fluorescence condenser lens for gathering the detector or the like issued included.

【0012】励起・受光光学系の一体化は、例えば、鏡筒の中に光源、検出器などを収容することにより行うが、これに限定されない。 [0012] Integration of excitation and light receiving optical system, for example, a light source into the barrel, is performed by accommodating a like detector is not limited to this.

【0013】一体化された励起・受光光学系を、ゲル電気泳動板に設置するときは、光学系の焦点が泳動レーンにくるようにすることは勿論である。 [0013] The integrated excitation and light receiving optical system, when installing the gel electrophoresis plate, it is obvious that the focus of the optical system is to come the electrophoresis lanes. 設置は、例えばマウント部材に励起・受光光学系を泳動レーンの間隔毎に複数個並べて、そのマウント部材を電気泳動板に嵌め合わせるようにすることが考えられるが、これに限定されず励起・受光光学系を直接ゲル電気泳動板の泳動レーンに設置しても良い。 Installation, for example, the mounting member to the excitation-light-receiving optical system are arranged a plurality at intervals of electrophoresis lanes, it is conceivable to make fitted the mounting member to the electrophoretic plate, the excitation and receiving is not limited thereto may be installed optical system directly to electrophoresis lanes of a gel electrophoresis plate. 設置方向も励起光がゲルに垂直に入射する方向には限定されず、斜入射する方向でも良い。 Installation direction is not limited to the direction which the excitation light is incident perpendicularly to the gel, may be in a direction oblique incidence.

【0014】なお、本発明で使用する蛍光ラベルとしては、例えば、イソチオシアン酸フルオレセイン(FIT [0014] Incidentally, as the fluorescent label to be used in the present invention, for example, fluorescein isothiocyanate (FIT
C)、イソチオシアン酸エオシン(EITC)、イソチオシアン酸テトラメチルローダミン(TMIRTC)、 C), isothiocyanate eosin (EITC), isothiocyanate tetramethylrhodamine (TMIRTC),
置換イソチオシアン酸ローダミン(XRITC)、テキアスレッドなどを挙げることができる。 Substituted isothiocyanate Rhodamine (XRITC), and the like enemy A thread. また、蛍光ラベルは塩基の種類、A(アデニン)、C(シトシン)、T The fluorescent labels are the type of base, A (adenine), C (cytosine), T
(チミン),G(グアニン)毎に異なるものを用いても良いし、同一のものを用いてもよい。 (Thymine), may be used different for each G (guanine), it may use the same thing. もし、塩基の種類毎に蛍光ラベルを異なえる場合(4種類の蛍光ラベルを用いる場合)は、一つの泳動レーンで4種類の塩基の測定が可能となる。 If (the case of using four kinds of fluorescent labels) if may differ fluorescent labels for each type of base, it is possible to measure the four bases in a single electrophoresis lane.

【0015】また、ゲル電気泳動には、キャピラリー型、平板型の両者を含む。 Further, the gel electrophoresis include capillary type, both of plate type.

【0016】 [0016]

【作用】本発明によれば、励起・受光光学系を一体化し、その光学系焦点を予め泳動レーンに一致させておくことにより、その一体化構造体をゲル電気泳動板に密着させることで、光学系の調整を必要としない構造となる。 According to the present invention, integrated excitation and light receiving optical system, by keeping to match the pre-migration lane the optics focus, by adhering the monolith structure to gel electrophoresis plate, a structure that does not require adjustment of the optical system.

【0017】また、泳動レーン毎に励起・受光光学系を1個設けているので、光学系の走査機能を排除できる。 Further, since the provided one of the excitation and light receiving optical system for each migration lane, it can be eliminated scanning function of the optical system.

【0018】 [0018]

【実施例】本発明の実施例を図面に基づいて説明する。 It is described with reference to examples of the embodiment of the present invention with reference to the drawings.
図1は、本発明にかかる装置の全体概略図で、1はゲル電気泳動板を示す。 Figure 1 is a schematic overall view of the device according to the present invention, 1 denotes a gel electrophoresis plate. これは、例えば厚さ5mmのパイレックスガラス板1a、1a´間に6%ポリアクリルアミドゲル1bを0.35mmの厚さに挟んで形成される。 This, for example, 5mm thick Pyrex glass plate 1a, is formed across the 6% polyacrylamide gel 1b to a thickness of 0.35mm between 1a '.

【0019】2は、ゲル電気泳動板に形成された凹状のウェルで、凹部に蛍光ラベルしたDNA断片が注入される。 [0019] 2 is a concave formed in the gel electrophoresis plate wells, fluorescence labeled DNA fragments are injected into the recess. 従って、このウェル2の凹部をゲル電気泳動板2の長さ方向に延長したのが泳動レーンとなる。 Therefore, the extended recess of the wells 2 of the gel electrophoresis plate 2 in the longitudinal direction is electrophoresis lanes. なお、ウェルの幅は典型的には5mm〜10mmの範囲にある。 Incidentally, the width of the well is typically in the range of 5 mm to 10 mm.

【0020】ゲル電気泳動板1の両端は緩衝液槽3、4 The opposite ends of the gel electrophoresis plate 1 buffer tank 3,4
に挿入され、この緩衝液槽3、4に一対の電極(図示せず)を入れて高電圧電源(図示せず)と接続する。 It is inserted into and connected to the pair of electrodes to the buffer tank 3 and 4 put (not shown) high-voltage power supply (not shown). 高電圧の印加によりウェル2に注入されたDNA断片は図の矢印方向に泳動する。 DNA fragment injected into the well 2 by applying a high voltage to migrate in the direction of the arrow in FIG. なお、緩衝液槽3、4に入れる緩衝液としては、例えば、リン酸緩衝液を用いる。 As the buffer solution placed in a buffer tank 3 and 4, for example, a phosphate buffer.

【0021】ゲル電気泳動板1の裏面には、泳動中にゲル電気泳動板を水冷する水冷装置5が設置されており、 [0021] On the back surface of the gel electrophoresis plate 1, a gel electrophoresis plate is water-cooled unit 5 for water-cooled are installed in the electrophoresis,
この水冷装置5は、例えば、平板に溝を切り、そこに水が流せるようになっている。 The water-cooling unit 5 is, for example, a flat plate cut grooves, and there become flown water. なお、水の導入出口は図示省略してある。 Incidentally, the inlet and outlet of water are not shown.

【0022】ゲル電気泳動板1での泳動方向(図の矢印方向)には、マウント部材6が嵌め込まれており、このマウント部材6にセンサヘッド7が複数個設置される。 [0022] direction of electrophoresis in the gel electrophoresis plate 1 (the arrow direction in the drawing), the mount member 6 is fitted, the sensor head 7 is mounted a plurality of this mount member 6.
センサヘッド7の間隔は、ウェルの幅に対応し、各泳動レーンに1個設置されることになる。 Distance of the sensor head 7, corresponding to the width of the well, will be placed one on each electrophoretic lane.

【0023】ここで、センサヘッド7の内部構造とゲル電気泳動板1の関係を図2に示す。 [0023] Here, the internal structure and the relationship of the gel electrophoresis plate 1 of the sensor head 7 in FIG. センサヘッド7は、 The sensor head 7,
光源であるLED71と検出器であるアバランシェ・フォトダイオード(APD)72を筒70内に固定している。 Is fixed to the avalanche photodiode (APD) 72 of the cylinder 70 is LED71 and the detector is a light source. LED71から発せられる光は干渉フィルタ77を通り、不必要波長がカットされた後、集光レンズ73で集光しゲル1bに照射される。 Light emitted from LED71 passes through the interference filter 77, after the unnecessary wavelengths are cut, is irradiated to the condensing gel 1b by the condenser lens 73. 集光レンズ73の焦点はゲル1bに合致されているのは勿論のことである。 The focal point of the condenser lens 73 is of course that what is matched to the gel 1b. 蛍光ラベルしたDNA断片から発せられる蛍光は、接眼レンズ74で集光し平行光にした後、干渉フィルタ及び色フィルタ75、集光レンズ76を通り、APD72に入射されるようになっている。 Fluorescence emitted from the fluorescent labeled DNA fragment, after the focused by the eyepiece 74 parallel light through an interference filter and a color filter 75, a condenser lens 76, and is incident on the APD72.

【0024】なお、使用するLEDの種類・波長等は、 [0024] In addition, the type and wavelength or the like of the LED to be used,
蛍光ラベルの種類により適宜選択される。 It is selected depending on the kind of a fluorescent label. 例えば、蛍光ラベルとしてテキサスレッドを用いる場合は590nm For example, when using Texas Red as a fluorescent label 590nm
を中心とする光を発するLEDを用い、干渉フィルタ7 An LED that emits light centered on the interference filter 7
7で610nmより長波長の光をカットする。 Cutting the light of wavelengths longer than 610nm at 7. また、前記の蛍光ラベルの場合、蛍光ラベルより発せられる蛍光は中心波長615nmのものとなり、フィルタ75で6 Also, in the case of the fluorescent label, fluorescence emitted from the fluorescent label is a thing of the central wavelength 615 nm, the filter 75 6
10nm以下の波長をカットして、APD72に入るようにする。 10nm to cut wavelengths below, to enter the APD72.

【0025】センサヘッド7からの信号はCPU(図示せず)に導かれ、そこで信号処理されて塩基配列が決定される。 The signal from the sensor head 7 is guided to a CPU (not shown) where the signals processed by the nucleotide sequence is determined.

【0026】なお、センサヘッドの構造は図2のものには限定されず、図3〜図5のものでも良い。 [0026] The structure of the sensor head is not limited to that of FIG. 2 may be of FIGS. 図3は、図2のものとは接眼レンズが異なり、半球状の接眼レンズ78を用いている。 Figure 3 is a different eyepiece to that of Figure 2, using an eyepiece lens 78 of the hemispherical. これにより、接眼レンズ78は蛍光の集光レンズとしての機能だけでなく、LED71の集光レンズを兼ねることにもなり、部品点数を少なく出来るとともに、等方的に発せられる蛍光に対し、立体角を広く集光できることになり、光学系が明るくなる。 Thus, the eyepiece 78 is not only a function as a condenser lens of the fluorescence, also will be serving as a condenser lens LED 71, with the number of parts reduced can, to the fluorescence emitted isotropically, solid angle will be able to widely condensing, the optical system is bright.

【0027】図4は、センサヘッド7の設置面と反対側に反射板(Al反射鏡)79を配置しており、これにより、ゲル電気泳動板を通過した光(入射光、蛍光の両方)を再度利用できることになり、光利用効率を高めることができる。 [0027] Figure 4, the reflecting plate on the opposite side of the mounting surface of the sensor head 7 is arranged (Al reflector) 79, thereby, the light passing through the gel electrophoresis plate (incident light, both fluorescence) will be available again, it is possible to increase the light use efficiency.

【0028】図5は、図4の変形で、ガラス板1a´のゲル側にAl80を蒸着しておくことにより、図4と同様の効果をもたらしている。 [0028] Figure 5 is a variant of FIG. 4, by the gel side of the glass plate 1a' keep depositing Al80, has led to the same effect as FIG.

【0029】なお、図3〜図5中、図2と同じものには同じ番号が付されている。 [0029] Incidentally, in FIGS. 3 to 5, the same numbers are assigned to the same thing as FIG.

【0030】 [0030]

【発明の効果】本発明によれば、泳動レーン毎に励起・ According to the present invention, excitation in each electrophoresis lane
受光光学系を1個設けているので、光学系の走査機能を排除できる。 Since is provided one light-receiving optical system, it can be eliminated scanning function of the optical system.

【0031】また、励起・受光光学系を一体化し、その光学系焦点を予め泳動レーンに一致させてゲル電気泳動板に密着させているので、光学系の調整を必要としない。 Further, integrated excitation and light receiving optical system, so that the optical system focus to match the pre-migration lanes are in close contact with the gel electrophoresis plate, does not require adjustment of the optical system.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

【図1】本発明の装置の全体概略を示す図である。 1 is a diagram showing an overall schematic of the apparatus of the present invention.

【図2】本発明のセンサヘッドを示す図である。 2 is a diagram showing a sensor head of the present invention.

【図3】本発明のセンサヘッドの他の実施例図である。 Figure 3 is another embodiment view of the sensor head of the present invention.

【図4】本発明のセンサヘッドの他の実施例図である。 Figure 4 is another exemplary illustration of a sensor head of the present invention.

【図5】本発明のセンサヘッドの他の実施例図である。 Figure 5 is another embodiment view of the sensor head of the present invention.

【図6】従来装置の概略図である。 6 is a schematic diagram of a conventional device.

【図7】従来装置の欠点を示す図である。 7 is a diagram showing the drawbacks of the conventional apparatus.

【符号の説明】 DESCRIPTION OF SYMBOLS

1:ゲル電気泳動板 3、4:緩衝液槽 7:センサヘッド 71:LED 72:APD 1: Gel Electrophoresis plates 3 and 4: Buffer tank 7: sensor head 71: LED 72: APD

Claims (1)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】 蛍光ラベルしたDNA断片を複数のレーンでゲル電気泳動させ、泳動方向と交わる方向に蛍光の励起・受光光学系を配置し、DNA断片の泳動パターンを検出する塩基配列決定装置において、 前記励起・受光光学系を一体化して、各泳動レーン毎に配置したことを特徴とする塩基配列決定装置。 1. A by gel electrophoresis fluorescent labeled DNA fragments by a plurality of lanes, the fluorescence excitation and light receiving optical system are arranged in a direction crossing the direction of electrophoresis, the sequencing device for detecting the electrophoresis pattern of the DNA fragments the excitation-integrated light-receiving optical system, the base sequence determining apparatus characterized by being arranged for each migration lane.
JP5270514A 1993-10-28 1993-10-28 Deciding apparatus for base sequence Pending JPH07120392A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5270514A JPH07120392A (en) 1993-10-28 1993-10-28 Deciding apparatus for base sequence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5270514A JPH07120392A (en) 1993-10-28 1993-10-28 Deciding apparatus for base sequence

Publications (1)

Publication Number Publication Date
JPH07120392A true JPH07120392A (en) 1995-05-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP5270514A Pending JPH07120392A (en) 1993-10-28 1993-10-28 Deciding apparatus for base sequence

Country Status (1)

Country Link
JP (1) JPH07120392A (en)

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US8557566B2 (en) 1998-05-16 2013-10-15 Applied Biosystems, Llc Instrument for monitoring polymerase chain reaction of DNA
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US8492138B2 (en) 1999-05-17 2013-07-23 Applied Biosystems, Llc Optical instrument including excitation source
US8557569B2 (en) 1999-05-17 2013-10-15 Applied Biosystems, Llc Optical instrument including excitation source
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US7387891B2 (en) 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US7423750B2 (en) 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
US7635588B2 (en) 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
US8809040B2 (en) 2002-05-17 2014-08-19 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
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