JPH0687752A - Antimicrobial water and its production - Google Patents

Abstract

PURPOSE:To obtain antimicrobial water useful for preventing and treating dental diseases and food poisoning, comprising an extracted solution of humus soil, having a specific pH value. CONSTITUTION:Warm water or a diluted alcohol solution at 30-60 deg.C is blended with 10-15 pts.wt. humus soil, preferably humus soil having a relatively high content of fulvic acid, stirred several times daily for at least three days, allowed to stand for 4-5 days as it is and the blend is precipitated. A supernatant liquid is filtered to give antimicrobial water having at least pH 4.0, preferably pH about 3.7. The antimicrobial water shows extremely excellent inhibitory effects on multiplication of Streptococcus mutans as a bacterium to cause tooth decay or Fusobacterium a bacterium to cause periodontal disease, has excellent safety and is useful as a gargle solution for preventing tooth decay of infants or a therapeutic solution for chronic periodontal disease. The antimicrobial water shows excellent inhibitory effects on bacteria to cause food poisoning such as Vibrio parahaemolyticus and is a hand cleaning solution or a food cleaning solution of an operator of foods.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to antibacterial water and a method for producing this antibacterial water, the purpose of which is to improve bacteria against dental diseases such as tooth decay and periodontal disease or bacteria causing food poisoning. In addition to showing antibacterial action and antibacterial action, it is extremely safe and can be continuously used for a long time with peace of mind as a daily mouthwash, or as a washing solution or dishwashing solution for food workers. It is intended to provide antibacterial water that can effectively contribute to prevention and treatment and prevention of food poisoning, and a manufacturing method that can obtain the antibacterial water very simply and efficiently.

[0002]

BACKGROUND OF THE INVENTION Caries (dental caries) and periodontal disease are considered to be two major diseases in the field of dentistry, and the morbidity of dental caries among Japanese people is extremely high compared to the dental caries situation in countries all over the world. ing. In addition, the prevalence of periodontal disease has increased with aging, but recently, the number of young people who suffer from periodontal disease has been increasing.

The cause of caries remains unclear, but it has been found that plaque formed on the surface of the tooth is largely involved. This dental plaque is formed by the action of certain oral bacteria on sucrose in food, which causes the adhesive dextran-like polysaccharide to be secreted out of the cells and causes bacterial aggregation on the tooth surface. It is said that lactic acid, which is continuously produced by bacteria in dental plaque, causes demineralization of tooth enamel surface and causes tooth decay.

On the other hand, periodontal disease is a general term for diseases in the base tissues supporting the teeth, that is, periodontal ligament, gingiva, and alveolar bone. In a narrow sense, it is largely divided into gingivitis and periodontitis (alveolar pyorrhea). Be separated. Gingivitis is an inflammation of only the gingiva, which causes swelling and soreness in the gums, and the further development of this symptom is periodontitis (alveolar pyorrhea). The cause of periodontal disease is due to impurities in the oral cavity, such as plaque and tartar, but it is said that approximately 1 to 200 million bacteria are present in approximately 1 mg of this plaque, and it is specific to the formation of plaque. Bacteria are greatly involved. Tartar is formed by depositing free calcium in saliva on the plaque. The bacteria and bacterial enzymes in the plaque and tartar cause inflammation of the mucous membrane in the gingival sulcus, and Pseudomonas aeruginosa infects the gingival sulcus to form an ulcer, resulting in periodontal disease. This periodontal disease, especially periodontitis (alveolar pyorrhea), has almost no subjective symptoms in the initial stage, and the base tissue of the tooth is destroyed over a long period of time, and blood and pus are excreted from the tissue, which eventually leads to It is a disease that causes teeth to fall out, and it is a horrifying disease that may be too late when subjective symptoms appear. About 50% of the cause of tooth loss is this periodontitis (alveolar pyorrhea). There is.

[0005]

2. Description of the Related Art As a method for treating and preventing dental diseases such as dental caries (dental caries) and periodontal disease mentioned above, a method of suppressing the growth of plaque, dental caries, and bacteria causing inflammation formation is adopted. ing. Streptococcus mutans is a bacteria involved in caries formation.
s), Streptococcus Michiisu (Streptococcus
mitis ) is known, and as bacteria involved in periodontal disease, Bacteroides gingivalis (Bactero
ides gingivalis) , Fusobacterium nucleatum
Anaerobic bacilli such as (Fusobacterium nucleatum) are known. Conventionally, in order to suppress the growth of these bacteria, administration of antibiotics or organic synthetic bactericides, or cleaning of teeth with mouthwashes containing these substances have been performed.

[0006]

However, there is a problem that the administration of the above-mentioned antibiotics and organic synthetic bactericides is difficult to apply to the prevention and treatment of dental caries in infants from the viewpoint of safety. In addition, periodontal disease usually takes a chronic condition and thus requires long-term treatment, but if antibiotics or synthetic bactericides are administered over a long period of time, side effects due to these drugs may occur, and at the same time, these antibiotics may cause side effects. There was a problem that the administration disturbed the normal bacterial flora in the oral cavity and intestine, and could cause new adverse effects such as opportunistic infection.
Therefore, the industry is extremely safe for the human body,
It has been desired to create antibacterial water that can be used safely even for infants and children, and that has an excellent effect on dental diseases such as alveolar pyorrhea, which has a high risk of extraction of all teeth.

[0007]

In the present invention, an antibacterial water and an antibacterial water containing at least a humus soil extract having a pH value of 4.0 or less as an active ingredient and 3
10-15 in warm water or dilute alcohol solution at 0-60 ° C
Part by weight of humus soil is mixed and allowed to stir several times a day for at least 3 days, then left as it is for at least 4 to 5 days to wait for precipitation, and the resulting supernatant is filtered to a pH value of at least 4.0 or less. By providing a method for producing antibacterial water, which is characterized in that the antibacterial water thus obtained is obtained, the above conventional problems are solved.

[0008]

[Action] Streptococcus mutans (Strepto
coccus mutans ) and Fusobacterium nucleatum (Fusobacterium nucleatum) showing an extremely excellent growth inhibitory effect against bacteria in the oral cavity, which is a cause of tooth decay and periodontal disease, and is safe against humans. Since it is extremely expensive, it can be safely used as an eyelash liquid for infants for a long time, and can be effectively used for treatment and prevention of dental diseases. Furthermore, it exhibits an excellent antibacterial effect against food poisoning-causing bacteria such as Staphylococcus aureus, so it is also suitable for washing hands of food workers, dishwashing water, drinking water in areas with frequent food poisoning, etc. Can be used for

[0009]

The structure of the antibacterial water and the method for producing the antibacterial water according to the present invention will be described in detail below. First, the antibacterial water will be described in detail. The antibacterial water of the present invention is an extract extracted from humus soil and has a pH value of at least 4.0.
The following extract is contained as an active ingredient.

The humus soil used as a starting material in the present invention means a soil containing 20% or more humic substances,
Soil in which complex organic substance residues mainly composed of polymer compounds formed by condensation polymerization of organic components (carbohydrates, fats, proteins, lignin, etc.) in animal and plant remains decomposed by the action of soil microorganisms are deposited . It is known that the formation process of this humus is extremely complicated and diverse, and the composition of humus varies greatly depending on the soil generation environment and the passage of time. Thus, humus soils do not have a constant composition and represent one continuous state of soil that is gradually renewed through slow partial decomposition and resynthesis, but usually due to differences in their extraction methods,
The alkali-soluble part is divided into humic acid in the acid-insoluble part, fulvic acid in the acid-soluble part in the alkali-soluble part, and humic acid in the alkali-insoluble part.

The humus used in the present invention is not particularly limited, and any humus can be preferably used, but humus having a relatively high fulvic acid content has a high acid concentration in the obtained extract. , Because it is possible to easily obtain an extract with a pH of about 2.5 to 3.0, especially at a depth of 10 to 60 m below the surface soil of about 2 m from the soil surface of a basin-like wetland (former wetland). The humus that exists by forming a spongy liquid phase in the wide-ranging water state can be more preferably used. Extraction liquid extracted from such humus soil
The antibacterial water of the present invention has an H value of 4.0 or less. In the present invention, the reason why the pH value of the humus extract is set to 4.0 or less is that the humus extract having a pH value of 4.0 or less, more preferably about 3.7 is particularly Streptococcus mutans ( Streptococcus mutans ), Fusobacterium nucleatu
This is because it is based on the experimental knowledge of the present inventor that it has an extremely excellent antibacterial effect on the bacteria causing tooth decay and periodontal disease such as m) . Further, the extraction solvent is not particularly limited, but water is more preferably used from the viewpoint of safety and treatment from the viewpoint of treatment and prevention of dental diseases, but a dilute alcohol solution of about 5% is also preferably used in the present invention. be able to.

[0012] Such antibacterial water is used alone, or as a mouthwash by appropriately diluting it with tap water, or in a range that does not impair the effects of the present invention.
An auxiliary component such as a pH adjusting agent may be blended as necessary to be suitably used as a preventive or therapeutic liquid for dental diseases.

Next, the method for producing antibacterial water according to the present invention will be described in detail. First, warm water at 30-60 ° C or 5
% Dilute alcohol solution is filled, and humus of 10 to 15 parts by weight of the aqueous solution is mixed therein. The humus to be used is not particularly limited as described above, but humus having a relatively high fulvic acid content is preferable because the obtained extract has a high acid concentration. While the humus soil is mixed in the solution, the mixture is stirred several times a day for at least 3 days, and left as such for at least 4 to 5 days to wait for precipitation. The reason why the extract is allowed to stand for at least 4 to 5 days in this way is that the extract obtained by allowing it to stand for 4 to 5 days can exert a favorable effect on dental diseases such as tooth decay and periodontal disease. It is because of the experimental knowledge of. After the required time has passed, the resulting supernatant liquid is taken out and filtered to obtain the antibacterial water according to the present invention.

[0014]

EXAMPLES Next, the effects of the antibacterial water according to the present invention will be further clarified with reference to Examples, Comparative Examples and Test Examples. (Example 1) Using 140 kg of raw material humus (collected in the vicinity of Yatsugatake, Suwa-gun, Nagano Prefecture and air-dried), this humus was mixed in 1 Kl of water and stirred several times a day for 3 days to give 5 After standing for a day to wait for precipitation, the obtained supernatant was taken out and filtered to obtain the antibacterial water of Example 1. The antibacterial water had a pH value of 3.0. (Example 2) The antibacterial water obtained in Example 1 was boiled at 100 ° C for 10 minutes to obtain the antibacterial water of Example 2.

[0015]

[Test example]

(Test example 1) Analytical test (drinking water standard) Regarding the antibacterial water obtained in the above-mentioned Example 1, "standard criteria for foods, additives, etc. 1st food D each article soft drink" (Showa 34)
Test prescribed by the Ministry of Health and Welfare Notification No. 370) (turbidity, sediment, arsenic, lead, cadmium, tin, coliforms)
I went to The results are shown in Table 1.

[Table 1]

(Test Example 2) Analytical test (tap water standard) The antibacterial water obtained in Example 1 was diluted 200 times with tap water and subjected to a compositional analysis test according to the water quality standard stipulated by the Water Supply Act. The results are shown in Table 2.

[Table 2]

(Test Example 3) Antibacterial test (periodontal disease, cavities causing tooth decay) 10 outpatients of preventive dentistry at the Dental University Hospital (6 males)
Plaque was collected from the gingiva of the oral cavity of 4 females (average age 51.8 years) with a curette, and 0.85% saline (0.05
% TGC), sonicated for 30 seconds, and stirred with a mixer until uniform to give a sample solution. Blood agar (10% sheep defibrinated blood), Bacteroides selection medium, Mitis-salivarius as cell culture medium
ager medium (MS medium), S. mutans selective medium (M
SB medium), Fusobacterium selective medium (FM medium), Veillonella selective medium (VM medium), Lact
Eight types of media, an obacillus selection medium (SL medium) and an Actinomyces selection medium, were prepared as plates, and 0.1 ml of each of the sample solutions was inoculated into each of the eight types of media.
After that, the antibacterial water obtained in Examples 1 and 2 and tap water as Comparative Example 1 were used, and 0.1 m of each was placed on the medium in the plate.
l was added to each and evenly applied on the surface. This plate 37
7 in anaerobic condition (N ₂ : CO ₂ : H ₂ = 8: 1: 1) at ℃
Reacted for days. After 7 days, a plate having a number of colonies of 50 to 500 was selected and the number of each colony was measured.

The number of colonies in various selection media is shown in Table 3. In addition, the ratio of the number of colonies of each plate when the comparative example (tap water) is set to 100 is shown in Table 4, and the graph of this number is shown in Table 5. Regarding the number of colonies on the two plates, the number of colonies on both plates was larger than that of the comparative example (tap water) (+), and the number of colonies on both plates was the comparative example (tap water).
Those that were less than the above were classified as ( - ) and those that were neither were classified as ( + ), and the results are shown in Table 6.

[Table 3]

[Table 4]

[Table 5]

[Table 6]

(Test Example 4) The sample solution prepared in Test Example 3 was used in the same Fusobacterium selective medium (F) as in Test Example 3.
0.1 ml each of the M medium) and 0.1 ml of the antibacterial water of Example 1 and physiological saline as Comparative Example 2 were added to the medium, respectively, and incubated for 1 minute, and the incubation time was 660 nm. The relationship with the OD value (turbidity) was tested. The results are shown in Table 7. The relationship between the number of colonies and the OD value (turbidity) at 660 nm is shown in Table 8 as an index.

[Table 7]

[Table 8]

Test Example 5 The relationship between the concentration of antibacterial water and the antibacterial effect was tested. The concentration of the antibacterial water obtained in Example 1 was adjusted to a range of 0 to 100% by dilution,
Sample solution prepared in Test Example 3 above in Fusobacterium medium
After adding 0.1 ml of each concentration of antibacterial water to the plate prepared by inoculating 0.1 ml of each and reacting for 1 minute, the relationship between the concentration of antibacterial water and the OD value (turbidity) at 660 nm is shown. Tested. The results are shown in Table 9. Table 10 shows the relationship between the antibacterial bacteria multiplication inhibition rate and the concentration of antibacterial water at this time.

[Table 9]

[Table 10]

(Test Example 6) The antibacterial effect was examined by the reaction time between the antibacterial water and the medium. The antibacterial water obtained in Example 1 was placed on a plate prepared by inoculating Fusobacterium medium with 0.1 ml each of the sample solution prepared in Test Example 3 above.
0.1 ml each was added and reacted for 0 to 5 minutes. The relationship between the reaction time and the OD value (turbidity) at 660 nm was tested. The results are shown in Table 11.

[Table 11]

(Test Example 7) The antibacterial effect was tested by changing the pH value of antibacterial water. A hydrochloric acid buffer solution is added to the antibacterial water obtained in Example 1, and several kinds having different pH values within the range of pH 3.0 to 5.0, and Comparative Example 3 are the same as the antibacterial water of Example 1. Several kinds of hydrochloric acid buffer solutions having a pH value were prepared in the same manner, 0.1 ml each was added to a plate prepared by inoculating the Fusobacterium medium with the same sample solution as in Test Example 3 above, and the mixture was reacted at 660 nm. The relationship with the OD value (turbidity) was tested. The results are shown in Table 12.

[Table 12]

(Test Example 8) Antibacterial test against food poisoning bacteria (1) Test strain Staphylococcus au as a test strain
reus ATCC 6538P), Salmonella enteri
tidis NHL), Vibrio parahaemolytic
Three strains of us EB 102) were used. (2) Test bacterial solution The test bacterial strain was inoculated into a normal broth medium (in the case of Vibrio parahaemolyticus, 3% of salt was added), and 35
After culturing at ℃ for 18 hours, the bacterial concentration is reduced with sterile physiological saline.
It was adjusted to 10 ⁶ cells / ml and used as each test bacterial solution. (3) Test operation The antibacterial water obtained in Example 1 was sterilized physiological saline (3% salt was added in the case of Vibrio parahaemolyticus).
After diluting 100-1000 times and measuring pH, 10 of them
ml was collected in a test tube. 0.1m of the test bacterial solution in this test tube
l was added to each well, and the mixture was allowed to stand at 35 ° C. for 2 hours, and then inoculated on a standard agar medium (3% salt was added in the case of Vibrio parahaemolyticus), and the number of bacteria was measured. As a comparative example, the same test was performed using tap water instead of the antibacterial water of Example 1. The results are shown in Table 13.

[Table 13]

Based on the results of Table 13, the reduction rate of the number of bacteria was calculated according to the following formula 1. The results are shown in Table 14.

[Formula 1]

[Table 14]

Test Example 9 Alveolar pus such as bleeding from the gums during eating, pain in the gums, poor gum color, loose gums, swelling, and bad breath Males and females aged 30 to 60 with leaky symptoms
The names (10 boys and 10 girls) were prepared by continuously using the antibacterial water of Example 1 in tap water to adjust the pH value to about 3.7 in the morning and evening as a toothpaste cleaning solution for 1 to 6 months. It was examined every 2 months for improvement of symptoms. The results are shown in Table 15.

[Table 15]

From the results in Table 1, the antibacterial water of the present invention satisfies the standard as "soft drink water", and from the results in Table 2, 100 times diluted solution must meet the water quality standard stipulated by the Water Supply Act. It is clear that there is no danger to the human body. As is clear from Tables 3 to 6,
The antibacterial water according to the present invention is Streptococcus mutans or Fusobacterium.
(Fusobacterium), bacteria causing dental caries such as Bacteroides (Bacteroides), it can be seen that with a growth inhibitory effect on bacteria causing periodontal disease. As is clear from Table 8, the OD value
It can be seen that the logarithm of the number of colonies on the plate is proportional to the OD value at 0.1 or more. Therefore, as is clear from Table 7, it is found that the antibacterial water of Example 1 has suppressed bacterial growth as compared with physiological saline. As is clear from Tables 9 to 10, the antibacterial count of Example 1 according to the present invention was found to be proportional to the concentration of the antibacterial growth, especially when the concentration was 50% or more. As is clear from Table 11, when the reaction time of the antibacterial water and the test medium is less than 1 minute, the effect of suppressing the growth of bacteria is enhanced as the reaction time becomes longer, and the reaction time of 1 minute to 4 minutes increases the reaction time. It can be seen that the suppression effect is almost constant regardless of. As is clear from the results in Table 12, it is understood that when the reaction time is 1 minute and the pH of the antibacterial water is 3.5 or less, there is a remarkable effect of suppressing bacterial multiplication as compared with the comparative example. As is clear from the results in Table 14, it is understood that the antibacterial water of the present invention has the effect of reducing the number of bacteria against food poisoning bacteria. It can be seen that Vibrio parahaemolyticus, which particularly favors the environment on the alkaline side, has a remarkable effect of reducing the number of bacteria. From the results in Table 15, it can be seen that the use of the antibacterial water of the present invention improves the symptoms of alveolar pyorrhea (bleeding and pain in the gums during eating, bad breath, etc.).

[0027]

As described in detail above, the present invention is characterized by containing as an active ingredient a humus extract having a pH value of 4.0 or less, and 30 to 60 ° C. 10-1 in warm water or dilute alcohol solution
5 parts by weight of humus was mixed and allowed to stir several times a day for at least 3 days, and allowed to stand for at least 4 to 5 days to wait for precipitation, and the resulting supernatant was filtered to obtain at least pH.
Since the method for producing antibacterial water is characterized by obtaining antibacterial water having a value of 4.0 or less, the cause of dental diseases such as tooth decay and periodontal disease is clear, as is clear from the results of the test examples. It has an extremely good growth inhibitory effect against fungi and bacteria causing food poisoning and has no risk to the human body. Therefore, it may be used alone or appropriately diluted to prevent cavities in babies and chronic teeth. As a solution for treating perinatal disease and as a suitable growth inhibitory effect against food poisoning bacteria such as Vibrio parahaemolyticus, it can be safely used for a long time as a disinfectant for food workers' hands and dishwashing liquid, as drinking water in areas with frequent food poisoning. It can be used continuously throughout the year, preventing dental diseases and food poisoning.
It has an excellent effect that it can contribute to treatment.

Claims (5)

[Claims] 1. An antibacterial water containing a humus soil extract having a pH value of 4.0 or less as an active ingredient. 2. The antibacterial water according to claim 1, wherein the extract is a water extract. 3. The antibacterial water according to claim 1, wherein the extract is a dilute alcohol extract. 4. The antibacterial water according to claim 1, wherein the extract is boiled. 5. Mixing 10 to 15 parts by weight of humus in hot water or a dilute alcohol solution at 30 to 60 ° C. several times a day,
Allow to stir for at least 3 days, then at least 4-5
A method for producing antibacterial water, characterized by allowing the solution to stand for a day to wait for precipitation, and filtering the resulting supernatant to obtain antibacterial water having a pH value of at least 4.0.