JPH0636732B2 - Cell crushing device - Google Patents

Cell crushing device

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Publication number
JPH0636732B2
JPH0636732B2 JP21989189A JP21989189A JPH0636732B2 JP H0636732 B2 JPH0636732 B2 JP H0636732B2 JP 21989189 A JP21989189 A JP 21989189A JP 21989189 A JP21989189 A JP 21989189A JP H0636732 B2 JPH0636732 B2 JP H0636732B2
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JP
Japan
Prior art keywords
rotary shaft
shaft
inclined
outer circumference
annular body
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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JP21989189A
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Japanese (ja)
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JPH0383574A (en
Inventor
亨 中山
數幸 吉岡
輝夫 天知
修二 安井
Original Assignee
安井器械株式会社
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Application filed by 安井器械株式会社 filed Critical 安井器械株式会社
Priority to JP21989189A priority Critical patent/JPH0636732B2/en
Publication of JPH0383574A publication Critical patent/JPH0383574A/en
Publication of JPH0636732B2 publication Critical patent/JPH0636732B2/en
Anticipated expiration legal-status Critical
Application status is Expired - Lifetime legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting

Abstract

PURPOSE:To enable the disintegration of cells of a number of specimens at a time by providing a rotary shaft with an inclined shaft part, fitting a ring to the inclined shaft in a state to keep the ring from rotation together with the rotary shaft and attaching closed vessels to the outer circumference of the ring. CONSTITUTION:A ring member 15 freely rotatable relative to an inclined shaft 8 clamped and fixed to a rotary shaft 6 is fit to the outer circumference of the inclined shaft 8 via a pair of bearings 13. A closed vessel 20 containing various kinds of cells to be disintegrated and micro-beads of 0.1-0.5mm in diameter is attached between the holder pieces 18a, 18b on the outer circumference of the ring member 15. A number of protrusion 17 are radially protruded from the outer circumference of the ring member 15 at a prescribed pitch in circumferential direction and a tension spring 23 is extended between a spring hook 22 of the protrusion and a mount 3 to prevent the rotation of the ring member 15 together with the rotary shaft 6. The rotary shaft 6 is rotated at 1,200-1,800rpm with a motor 5 to effect the swinging motion of the ring member to both sides in the direction of shaft center. The closed vessel 20 is oscillated in the form of figure 8 according to the high-speed rotation of the rotary shaft 6 to disintegrate the cells in the closed vessel 20 with the micro-beads.

Description

【発明の詳細な説明】 (産業上の利用分野) 本発明は細胞や組織を破砕する細胞破砕装置に関するものである。 BACKGROUND OF THE INVENTION (FIELD OF THE INVENTION) The present invention relates to a cell crushing device for crushing the cell or tissue.

(従来の技術) 酵母菌、バクテリア等の物質生産菌や生産細胞等のスクリーニング用の細胞破砕装置としては、高速回転刀による破砕、超音波による破砕、圧力による破砕等の種々の破砕方式を採用したものが知られているが、その中で被破砕細胞とガラスやセラミックス製の微小ビーズを密封容器に収容してこの密封容器を高速で振動させ、微小ビーズと細胞の衝突を繰り返して細胞を破砕する方式のものも知られている。 The (prior art) cell disruption device for screening of yeast, such as substance producing strain and production cells such as bacteria, adopted crushing by high-speed rotation sword, disrupted by ultrasonic waves, various crushing method crushing due Pressure Although there has been known those, to be disrupted cells and glass or ceramic micro-beads therein housed in a sealed container is vibrated the sealed container at a high speed, the cells repeatedly hits microbeads and cells It is also known that of crushing the system.

(発明が解決しようとする課題) ところが、従来の微小ビーズ衝撃方式による破砕装置においては、単一の密閉容器をセットできるように構成されており、多数のサンプルの細胞破砕を行う場合には大変時間がかかり、能率が悪いという問題があった。 (Problems to be Solved) However, in the crushing device according to the conventional microbead impact system is configured to set a single closed container, very when performing cell disruption of multiple samples time-consuming, efficiency is poor.

そこで、多数の容器をセットできるように振動体上に多数の容器ホルダを配置した構成も考えられるが、そのような振動体に最適な振動形態である8の字状の振動を行わせようとすると、構成が複雑で大型になるという問題があった。 Therefore, it is considered configuration of arranging the plurality of container holder to a number of the vibrating body so that it can set the container, attempts performed shaped vibration of 8 is the best vibration form such vibrating body and Then, there is a problem that the configuration becomes complicated and large.

本発明は上記従来の問題点に鑑み、多数のサンプルの細胞破砕を一度に行うことができるとともに、簡単な構成で振動形態が最適な8の字状となる細胞破砕装置を提供することを目的とする。 In view of the above problems, aims it is possible to perform the cell disruption of multiple samples at one time, the vibration mode with a simple structure to provide a shape to become cells crushing device optimal 8 to.

(課題を解決するための手段) 本発明の細胞破砕装置は、上記目的を達成するため、回転駆動可能な回転軸にその軸心に対して中心線が傾斜した傾斜軸部を設け、この傾斜軸部に中心線が一致するように環状体を相対回転自在に外嵌するとともに、この環状体にその回転を弾性的に拘束するバネを係着し、前記環状体の外周に、被破砕物とビーズを収容した密閉容器を保持するホルダを周方向に多数配置したことを特徴とする。 Cell disruption apparatus (Means for Solving the Problems) In order to achieve the above object, provided the inclined shaft portion centerline is inclined relative to the axis in rotationally drivable rotary shaft, the inclination with the center line in the axial section is fitted relatively rotatably an annular body to match, a spring for restraining the rotation elastically in the annular body is engaged, the outer circumference of the annular body, the crush material wherein the plurality is arranged and the holder for holding the sealed container containing a bead in the circumferential direction.

(作用) 本発明の細胞破砕装置によれば、回転軸に設けた傾斜軸部に対し、環状体が相対回転自在に外嵌され、環状体が回転軸と共回りしないように、その回転がバネによって弾性的に拘束された構成となっており、傾斜軸部ひいては環状体の中心線が回転軸の軸心に対して傾斜しているので、回転軸が1回転するたびに環状体の外周の各点は8の字状に移動する。 (Operation) According to the cell crushing device of the present invention, with respect to the inclined shaft portion provided on the rotary shaft, the annular body is fitted to be relatively rotatable, so that the annular body is not rotating together with the rotating shaft, the rotation the spring has a structure which is elastically restrained by, since the center line of the inclined shaft portion thus annular body is inclined to the axis of the rotary shaft, the outer circumference of the annular body each time the rotary shaft rotates 1 each point moves to the 8-shaped. 従って、回転体を高速回転させると、環状体の外周にホルダを介して配置した密閉容器の内容物(被破砕物とビーズ)は8の字状の振動形態で振動せしめられ、被破砕物はビーズによる効果的な衝突、 Therefore, when the high speed rotation of the rotary member, the contents of the closed vessel disposed through a holder on the outer circumference of the annular body (object to be crushed and beads) is being vibrated in shaped vibration state of 8, the crush material is effective collision by the beads,
摩擦作用を受け、短時間で効率的に破砕される。 Receiving a rubbing action, it is effectively crushed in a short time.

その上、環状体の外周にはホルダを多数配置してあるので、一度に多数のサンプルの細胞破砕を行うことができる。 Moreover, since the outer circumference of the annular body is disposed a large number of holders, it is possible to perform the cell disruption of multiple samples at one time.

(実施例) 以下、本発明の一実施例を第1図〜第4図を参照しながら説明する。 (Example) will be described with an embodiment of the present invention with reference to Figure 1-Figure 4.

1はベースで、その上に防振ゴム2を介して架台3が設置されている。 1 is the base, the platform 3 via the anti-vibration rubber 2 is installed thereon. この架台3上には、一対の軸受ユニット4a、4bとモータ5がそれらの軸心が一直線上に位置するように配置されている。 The platform 3 on a pair of bearing units 4a, is 4b and the motor 5 is their axis are arranged to be located on a straight line. 一対の軸受ユニット4a、 A pair of bearing units 4a,
4bは互いに適当な間隔を設けて配置された軸受台3 4b is bearing stand 3 which is arranged with a mutually appropriate intervals
a、3b上に設置されており、回転軸6の両端部を回転自在に支持している。 a, is installed on a 3b, rotatably supports both end portions of the rotation shaft 6. この回転軸6の一端6aはカップリング7を介して前記モータ5の出力軸5aに結合されている。 One end 6a of the rotating shaft 6 is coupled to the output shaft 5a of the motor 5 via a coupling 7. 前記回転軸6の軸受ユニット4a、4b間には、中心軸心に対して中心線が傾斜した傾斜孔8aを形成された傾斜軸体8が外嵌されるとともに、キー(図示せず)にて相対回転不可能に結合されている。 Bearing unit 4a of the rotary shaft 6, between 4b, together with the inclined shaft 8 which center line is formed an inclined hole 8a which is inclined is fitted with respect to the central axis, the key (not shown) It is relatively non-rotatably coupled Te. この傾斜軸体8は、その両端面に当接配置された傾斜スペーサリング9a、9bを介して、回転軸6の外周に突設された鍔部10と回転軸6の外周面に形成されたねじ部11に螺合された固定ナット12とにより軸心方向にも狭圧固定され、回転軸6と一体的に回転する傾斜軸部を構成している。 The inclined shaft 8 is inclined spacer ring 9a which is in contact disposed on both end faces thereof, through 9b, formed on the outer circumferential surface of the rotating shaft 6 and the collar portion 10 projecting from the outer periphery of the rotary shaft 6 also narrow pressure fixed in the axial direction by a fixing nut 12 screwed on the threaded portion 11 constitute the inclined shaft portion rotates integrally with the rotary shaft 6.

傾斜軸体8の外周には、一対の軸受13を介して相対回転自在に環状体15が外嵌されている。 The outer periphery of the inclined shaft 8 is relatively rotatably annular body 15 via a pair of bearings 13 are fitted. 14は傾斜軸体8側の軸受固定ナット、16は環状体15側の軸受固定ナットである。 14 inclined shaft 8 side of the bearing fixing nut, 16 is a bearing lock nut of the annular body 15 side. 環状体15の外周には、周方向に所定ピッチで放射状に多数(図示例では12個)の突部17が突設されている。 The outer periphery of the annular body 15, projections 17 of multiple radially at a predetermined pitch in a circumferential direction (twelve in the illustrated example) is projected. この突部17の両端面には、それぞれ径方向外方に突出しかつ互いに対向するように弾性板から成るホルダ片18a、18bがビス19にて取付けられ、これらホルダ片18a、18b間でチューブ状の密閉容器20を着脱自在に保持できるように構成されている。 On both end faces of the projections 17, respectively protrude radially outward and the holder pieces 18a of elastic plate so as to face each other, 18b is attached at screws 19, these holders pieces 18a, the tubular between 18b It is configured to be hold the sealed container 20 detachably.

2つの隣合う突部17、17間には接続部21が形成されるとともにその外周面が平坦にされてバネ掛け22が取付けられ、このバネ掛け22と架台3との間に引張バネ23が介装され、環状体15が回転軸6と共回りしないように回転を阻止している。 Between two adjacent protrusions 17 and 17 is mounted a spring hook 22 the outer peripheral surface thereof is flattened together with the connection portion 21 is formed, spring 23 tension between the spring hook 22 and the platform 3 is is interposed, it is prevented from rotating so that the annular body 15 does not rotate together with the rotary shaft 6. さらに、接続部21で互いに接続されている一方の突部17とその一側方に隣接する突部17との間に形成されている空間の中央部を通るストッパ軸24が設けられ、その両端部は軸受け台3 Further, the stopper shaft 24 passing through the center portion of the space formed between one protrusion 17 which are connected to each other at the connection portions 21 and the projections 17 adjacent to the one side is provided, both ends parts are bearing base 3
a、3bにて支持されている。 a, and it is supported by 3b.

次に、動作を説明する。 Next, the operation will be described. サンプルとしての各種被破砕細胞とガラスやセラミックス製の微小ビーズをそれぞれチューブ状の密閉容器20内に収容し、この密閉容器20 Various be crushed cells as the sample and the glass or ceramic micro-beads, respectively housed in a tubular closed container 20, the sealed container 20
を環状体15の外周の各ホルダ片18a、18b間に保持させる。 Each holder pieces 18a of the outer circumference of the annular body 15, is held between 18b. 前記微小ビーズは、直径0.1〜0.5mmのガラスビースやジルコニア等のセラミックスビーズが、 The microbeads, ceramics beads glass beads or zirconia having a diameter of 0.1 to 0.5 mm,
例えば微生物の破砕や真核細胞の破砕等の破砕目的に応じて選択して使用される。 Are selected and used in accordance with the crushing purposes, for example crushed like crushing or eukaryotic microorganisms.

密閉容器20の装着が終わると、モータ5を駆動し、回転軸6を例えば1200〜1800rpmで高速回転させる。 When the mounting of the sealed container 20 is completed, it drives the motor 5, is rotated at high speed rotary shaft 6 for example by 1200~1800Rpm. すると、環状体15が回転軸6と一体回転する傾斜軸体8の外周に相対回転自在に外嵌され、かつ引張バネ23にて共回転を阻止させているので、第3図に示すように、回転軸6の回転が1回転する毎に環状体6が軸心方向両側に振れ運動する。 Then, the annular member 15 is thereby prevented co-rotating with the rotary shaft 6 and relatively rotatably fitted on the outer periphery of the inclined shaft 8 which rotates integrally, and the tension spring 23, as shown in FIG. 3 , the rotation of the rotary shaft 6 is an annular body 6 is deflecting motion in the axial direction on both sides in each rotation of.

このとき、環状体15の外周の任意の点は、第4図 In this case, any point on the outer circumference of the annular body 15, FIG. 4
(a)、(b)に示すように8の字状に移動することになる。 (A), it will move as the 8-shaped shown in (b).
即ち、第4図(a)に実線で示すように環状体15が紙面の上下方向に傾斜した状態を基準位置として、そのときの環状体15の外周上におけるa点位置の挙動を見てみると、実線状態から回転軸6が矢印方向に90°回転すると、環状体15は紙面の表裏方向にも僅かに動きつつ、仮想線に示すような状態に移行し、その間a点に対応していた位置は円弧状の経路bを経てc点に移動する。 That is, as a reference position a state where the annular body 15 is tilted in the vertical direction of the page as indicated by the solid line in 4 (a), looking at the behavior of a point position on the outer peripheral on the annular body 15 at that time If, when the rotary shaft 6 from the solid line state is rotated 90 ° in the direction of the arrow, the annular body 15 while movement slightly in front and back direction of the page, and shifts to a state as shown in phantom lines, support therebetween a point position is moved to point c through an arcuate path b. 次に、回転軸6がさらに90°回転すると、環状体15は第4図(b)に実線で示すような状態に移行し、a Then, when the rotary shaft 6 is further 90 ° rotation, the annular member 15 moves to a state as shown by the solid line in FIG. 4 (b), a
点に対応していた位置はc点から円弧状の経路dを経て元のa点に戻る。 Position corresponds to point back to the original point a through the arcuate path d from point c. さらに回転軸6が90°回転すると、 Further rotation shaft 6 is rotated 90 °,
環状体15は仮想線で示すような状態に移行し、a点に対応していた位置は円弧状の経路eを経てf点に移動し、さらに回転軸6が元の回転位置まで90°回転すると、a点に対応していた位置f点から円弧状の経路gを経て元のa点に戻るのである。 Annular body 15 moves to a state as shown in phantom, the position corresponded to a point moves to the point f via an arcuate path e, further rotation shaft 6 is rotated 90 ° to its original rotational position then, it is back to the original point a through the arcuate path g from the position f point corresponds to a point. 前記円弧状の経路b、 The arcuate path b,
d、e、gの軌跡は、前記引張りバネ23の弾性的拘束力と、環状体6に対する傾斜軸体8の摩擦抵抗とのバランスによって定まり、前記弾性的拘束力が大きい場合には前記軌跡は直線状態に近づくこととなる。 d, e, the trajectory of g is resiliently restraining force of the tension spring 23, determined by the balance between the frictional resistance of the tilt shaft 8 relative to the annular body 6, the trajectory when the elastic restraining force is large so that the closer to the straight line state.

従って、環状体15の外周に保持された密閉容器20は回転軸6の高速回転に伴って8の字状の振動形態で振動せしめられ、その結果密閉容器20内で被破砕細胞に微小ビーズが効果的に衝突し、その衝突によって速やかにかつ均一に細胞が破砕される。 Thus, the sealed container 20 held on the outer periphery of the annular body 15 is vibrated in the shape of the vibration form of 8 with the high-speed rotation of the rotary shaft 6, is the result sealed container 20 within a minute beads to be disrupted cells effectively collide, rapidly and uniformly cells by the collision is crushed. こうして、多数の密閉容器20内に収容された多数のサンプルの細胞破砕を一度に破砕処理することができる。 Thus, it is possible to crushing at a time cell disruption of multiple samples contained in a large number of the sealed container 20.

尚、環状体15はその外周位置を引張バネ23にて弾性的に引張することによって、上記8の字状の変位動作を許容しながらその回転を阻止しているため、回転軸6の回転速度域によっては共振による異常振動を発生する恐れがあるが、その場合ストッパ軸24に突部17が当接するため、異常振動は確実に防止される。 Incidentally, by tensile elastically in the annular body 15 a spring 23 pulling the peripheral position, because it prevents its rotation while permitting shaped displacement movement of the 8, the rotational speed of the rotary shaft 6 Although the range is likely to cause abnormal vibration due to resonance, in which case projection 17 to the stopper shaft 24 to abut, abnormal vibration is reliably prevented.

上記実施例では、回転軸6上に単一の環状体15を配置した例を示したが、第5図に示すように回転軸6上に同一構成の複数の環状体15を並列配置してもよく、一層大量のサンプルの細胞破砕を一度に行うことができる。 In the above embodiment shows an example in which the single annular member 15 on the rotary shaft 6, and the fifth same configuration of a plurality of annular body 15 on the rotating shaft 6 as shown in FIG arranged in parallel also good, it can be carried out at a time more cell disruption of a large amount of sample.

又第1図に仮想線で示すように開閉可能なボックス50 The openable box 50 as shown in phantom in FIG. 1
で環状体15の全体を覆いここに冷気51を送って、破砕時の振動により昇温する密閉容器20の内部を冷却できるようにすれば好適である。 In sending here cold 51 covers the entire annular body 15, it is preferred to the inside of the sealed container 20 to raise the temperature by vibration during crushing to allow cooling. このボックス50は万が一密閉容器20がホルダ片18a、18bから離脱した場合にも、それが飛び散るのを防止する安全ボックスとしての作用をも営む。 The box 50 is any chance the sealed container 20 is holder piece 18a, even when detached from 18b, engaged also acts as a safety box to prevent it splashing.

(発明の効果) 本発明の細胞破砕装置によれば、環状体の周囲に配置した多数のホルダに密閉容器を保持させることによって一度に多数のサンプルの細胞破砕を行え、かつ回転軸に設けた傾斜軸部に、バネによる拘束によって共回転しないように嵌合した環状体の外周に各密閉容器を保持するので、各密閉容器はそれぞれ回転軸が1回転する度に8の字状に移動し、回転軸を高速回転させることによって各密閉容器を8の字状の最適な振動形態で振動させることができる。 According to the cell disruption apparatus (Effect of the Invention), can the number of samples of cell disruption at a time by holding the closed container into a number of holders arranged around the annular body, and provided on the rotating shaft the inclined shaft portion, so hold each sealed container the outer periphery of the annular body is fitted so as not to co-rotate by restraint of the spring, the closed container, respectively rotary shaft is moved to the 8-shaped every time one rotation , it can be vibrated at shaped optimal vibration state of each sealed container 8 by a high speed rotation of the rotary shaft. しかも、回転軸に傾斜軸部を設けて環状体を外嵌しただけの簡単な構成で安価に構成できる等、大なる効果を発揮する。 Moreover, such can be inexpensively configured the annular body provided with a inclined shaft portions to the rotary shaft with a simple configuration of just fitted, it exerts a large becomes effective.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

第1図〜第4図は本発明の一実施例を示し、第1図は部分断面正面図、第2図は側面図、第3図は動作状態を示す要部の正面図、第4図(a)、(b)は環状体の外周における振動形態の説明図、第5図は本発明の他の実施例の要部の正面図である。 Figure 1 - Figure 4 shows an embodiment of the present invention, FIG. 1 is a partial sectional front view, FIG. 2 is a side view, a front view of a main part Figure 3 is showing an operating state, FIG. 4 (a), a front view of a main part of (b) another embodiment of the illustration of the vibration mode in the outer periphery of the annular body, Fig. 5 present invention. 6……回転軸 8……傾斜軸体 15……環状体 18a、18b……ホルダ片 20……密閉容器 23……引張バネ 6 ...... rotary shaft 8 ...... inclined shaft 15 ...... annular body 18a, 18b ...... holder piece 20 ...... sealed container 23 ...... tension spring

Claims (1)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】回転駆動可能な回転軸にその軸心に対して中心線が傾斜した傾斜軸部を設け、この傾斜軸部に中心線が一致するように環状体を相対回転自在に外嵌するとともに、この環状体にその回転を弾性的に拘束するバネを係着し、前記環状体の外周に、被破砕物とビーズを収容した密閉容器を保持するホルダを周方向に多数配置したことを特徴とする細胞破砕装置。 1. A provided an inclined shaft portion centerline is inclined relative to the axis in rotationally drivable rotary shaft, fitted relatively rotatably an annular body so that the center line coincides with the inclined shaft portion as well as, that the annular body in its rotation by fastening the spring to elastically constrained, that the outer circumference of the annular body, and arranging a large number of holders for holding the sealed container containing an object to be crushed beads in the circumferential direction cell disruption apparatus according to claim.
JP21989189A 1989-08-25 1989-08-25 Cell crushing device Expired - Lifetime JPH0636732B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP21989189A JPH0636732B2 (en) 1989-08-25 1989-08-25 Cell crushing device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP21989189A JPH0636732B2 (en) 1989-08-25 1989-08-25 Cell crushing device

Publications (2)

Publication Number Publication Date
JPH0383574A JPH0383574A (en) 1991-04-09
JPH0636732B2 true JPH0636732B2 (en) 1994-05-18

Family

ID=16742664

Family Applications (1)

Application Number Title Priority Date Filing Date
JP21989189A Expired - Lifetime JPH0636732B2 (en) 1989-08-25 1989-08-25 Cell crushing device

Country Status (1)

Country Link
JP (1) JPH0636732B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009090292A (en) * 1999-10-13 2009-04-30 Yasui Kikai Kk Sample crushing tool
JP2010110234A (en) * 2008-11-04 2010-05-20 Shibata Kagaku Kk Method for extracting nucleic acid from microorganism cell using dry crushing
JP5371995B2 (en) * 2008-09-06 2013-12-18 平田機工株式会社 Sample crushing device

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4919664B2 (en) * 2006-01-18 2012-04-18 城戸 一正 Crusher
JP5663311B2 (en) * 2008-01-09 2015-02-04 ケック グラデュエイト インスティテュート Adjustment and / or handling system of a substance, the apparatus and method
CA2766517A1 (en) 2009-06-26 2010-12-29 Claremont Biosolutions Llc Capture and elution of bio-analytes via beads that are used to disrupt specimens
JP5351242B2 (en) * 2011-11-17 2013-11-27 有限会社興国産業 Crushing device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009090292A (en) * 1999-10-13 2009-04-30 Yasui Kikai Kk Sample crushing tool
JP5371995B2 (en) * 2008-09-06 2013-12-18 平田機工株式会社 Sample crushing device
JP2010110234A (en) * 2008-11-04 2010-05-20 Shibata Kagaku Kk Method for extracting nucleic acid from microorganism cell using dry crushing

Also Published As

Publication number Publication date
JPH0383574A (en) 1991-04-09

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