JPH0569466B2 - - Google Patents
Info
- Publication number
- JPH0569466B2 JPH0569466B2 JP62080470A JP8047087A JPH0569466B2 JP H0569466 B2 JPH0569466 B2 JP H0569466B2 JP 62080470 A JP62080470 A JP 62080470A JP 8047087 A JP8047087 A JP 8047087A JP H0569466 B2 JPH0569466 B2 JP H0569466B2
- Authority
- JP
- Japan
- Prior art keywords
- hemoglobin
- feces
- sample
- lytic enzyme
- lysozyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000001554 Hemoglobins Human genes 0.000 claims description 41
- 108010054147 Hemoglobins Proteins 0.000 claims description 41
- 210000003608 fece Anatomy 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 25
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 15
- 108010014251 Muramidase Proteins 0.000 claims description 10
- 102000016943 Muramidase Human genes 0.000 claims description 10
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 10
- 229960000274 lysozyme Drugs 0.000 claims description 10
- 235000010335 lysozyme Nutrition 0.000 claims description 10
- 239000004325 lysozyme Substances 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 230000001420 bacteriolytic effect Effects 0.000 claims description 4
- 238000003018 immunoassay Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 9
- 230000000968 intestinal effect Effects 0.000 description 9
- 230000001900 immune effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 229920001817 Agar Polymers 0.000 description 7
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 230000002550 fecal effect Effects 0.000 description 6
- 210000003097 mucus Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 230000000740 bleeding effect Effects 0.000 description 4
- 210000002249 digestive system Anatomy 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000008272 agar Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 108091007734 digestive enzymes Proteins 0.000 description 3
- 102000038379 digestive enzymes Human genes 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 102000013415 peroxidase activity proteins Human genes 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- CMPQUABWPXYYSH-UHFFFAOYSA-N phenyl phosphate Chemical compound OP(O)(=O)OC1=CC=CC=C1 CMPQUABWPXYYSH-UHFFFAOYSA-N 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8047087A JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8047087A JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS63246667A JPS63246667A (ja) | 1988-10-13 |
JPH0569466B2 true JPH0569466B2 (fr) | 1993-10-01 |
Family
ID=13719148
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8047087A Granted JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS63246667A (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019107414A1 (fr) | 2017-12-01 | 2019-06-06 | 栄研化学株式会社 | Procédé de stabilisation d'un complexe d'hémoglobine et d'haptoglobine et solution de conservation permettant de conserver des échantillons contenant de l'hémoglobine |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0259466U (fr) * | 1988-10-20 | 1990-05-01 | ||
JPH0260870U (fr) * | 1988-10-26 | 1990-05-07 | ||
EP3761030A4 (fr) | 2018-03-02 | 2022-03-02 | Eiken Kagaku Kabushiki Kaisha | Méthode de stabilisation d'une protéine contenue dans un échantillon et solution de stabilisation d'une protéine contenue dans un échantillon |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
JPS54157690A (en) * | 1976-07-06 | 1979-12-12 | Becton Dickinson Co | Plastic substrate coated with acceptor for ligand to be detected* and method of making said substrate |
JPS56106154A (en) * | 1980-01-17 | 1981-08-24 | Suovaniemi Finnpipette | Detection method of hemoglobin in night soil |
JPS5823796A (ja) * | 1981-07-16 | 1983-02-12 | エフ.ホフマン ― ラ ロシュ アーゲー | 人潜血の検出方法 |
JPS59125064A (ja) * | 1983-01-05 | 1984-07-19 | Eiken Kagaku Kk | 免疫学的ラテツクス凝集反応をもちいた糞便中のヘモグロビンの検出方法および該方法に使用する試薬 |
JPS59182367A (ja) * | 1983-02-02 | 1984-10-17 | オ−ストレイリアン・モノクロ−ナル・デベロプメント・プロプライエタリ−・リミテツド | 糞便中の微量血液を検出するための方法および診断器具 |
JPS61228351A (ja) * | 1985-04-02 | 1986-10-11 | Kyoto Ikagaku Kenkyusho:Kk | 糞便中のヘモグロビンの検出方法 |
JPS6261584A (ja) * | 1985-09-13 | 1987-03-18 | Tdk Corp | 生理活性物質固定化磁気微粒子 |
-
1987
- 1987-03-31 JP JP8047087A patent/JPS63246667A/ja active Granted
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
JPS54157690A (en) * | 1976-07-06 | 1979-12-12 | Becton Dickinson Co | Plastic substrate coated with acceptor for ligand to be detected* and method of making said substrate |
JPS56106154A (en) * | 1980-01-17 | 1981-08-24 | Suovaniemi Finnpipette | Detection method of hemoglobin in night soil |
JPS5823796A (ja) * | 1981-07-16 | 1983-02-12 | エフ.ホフマン ― ラ ロシュ アーゲー | 人潜血の検出方法 |
JPS59125064A (ja) * | 1983-01-05 | 1984-07-19 | Eiken Kagaku Kk | 免疫学的ラテツクス凝集反応をもちいた糞便中のヘモグロビンの検出方法および該方法に使用する試薬 |
JPS59182367A (ja) * | 1983-02-02 | 1984-10-17 | オ−ストレイリアン・モノクロ−ナル・デベロプメント・プロプライエタリ−・リミテツド | 糞便中の微量血液を検出するための方法および診断器具 |
JPS61228351A (ja) * | 1985-04-02 | 1986-10-11 | Kyoto Ikagaku Kenkyusho:Kk | 糞便中のヘモグロビンの検出方法 |
JPS6261584A (ja) * | 1985-09-13 | 1987-03-18 | Tdk Corp | 生理活性物質固定化磁気微粒子 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019107414A1 (fr) | 2017-12-01 | 2019-06-06 | 栄研化学株式会社 | Procédé de stabilisation d'un complexe d'hémoglobine et d'haptoglobine et solution de conservation permettant de conserver des échantillons contenant de l'hémoglobine |
Also Published As
Publication number | Publication date |
---|---|
JPS63246667A (ja) | 1988-10-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |