JPH05273218A - Dispensation - Google Patents

Dispensation

Info

Publication number
JPH05273218A
JPH05273218A JP6736392A JP6736392A JPH05273218A JP H05273218 A JPH05273218 A JP H05273218A JP 6736392 A JP6736392 A JP 6736392A JP 6736392 A JP6736392 A JP 6736392A JP H05273218 A JPH05273218 A JP H05273218A
Authority
JP
Japan
Prior art keywords
nozzle
sample
container
discharged
air layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6736392A
Other languages
Japanese (ja)
Other versions
JP2537584B2 (en
Inventor
Masaaki Hibino
Shinichi Igarashi
Masaaki Watabe
信一 五十嵐
正明 日比野
正明 渡部
Original Assignee
Aloka Co Ltd
アロカ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aloka Co Ltd, アロカ株式会社 filed Critical Aloka Co Ltd
Priority to JP4067363A priority Critical patent/JP2537584B2/en
Publication of JPH05273218A publication Critical patent/JPH05273218A/en
Application granted granted Critical
Publication of JP2537584B2 publication Critical patent/JP2537584B2/en
Anticipated expiration legal-status Critical
Application status is Expired - Lifetime legal-status Critical

Links

Abstract

PURPOSE:To move a nozzle without scattering sample balls left at the front end of the nozzle after a sample is discharged, by sucking a little amount of air after the sample is discharged and forming an air layer at the end of the nozzle. CONSTITUTION:When a predetermined amount of a sample 12 is sucked by a nozzle 10, the nozzle 10 is moved above a container 14, and then the nozzle 10 is descended. The nozzle is descended to the position where a sample 16 within the container is not in touch with the front end of the nozzle 10 even after the sample is discharged. A pump is driven after the nozzle 10 is descended, and the sample 12 in the nozzle 10 is discharged into the container 14. At this time, a sample bail 12a of the sample remaining at the inner wall of the nozzle 10 is adhered at the front end of the nozzle 10. Subsequently, the nozzle 10 is raised and the pump is driven to suck a predetermined amount of air. Since an air layer 20 is formed below the sucked sample 12a to seal the nozzle, it is prevented that the sample drips.

Description

【発明の詳細な説明】 DETAILED DESCRIPTION OF THE INVENTION

【0001】 [0001]

【産業上の利用分野】本発明は、自動分注装置において行われる液状試料の分注方法に関する。 The present invention relates to a dispensing method of the liquid sample to be performed in an automatic dispensing device.

【0002】 [0002]

【従来の技術】液状試料をノズルによって吸引し他の容器へ分注を行う自動分注装置が知られており、例えば人体から採取された血液などの分注を行うものとして用いられている。 BACKGROUND OF THE INVENTION liquid sample aspirated by the nozzle has an automatic dispensing apparatus that performs dispensing are known to the other vessel, it has been used as performing dispensing, such as blood taken for example from a human body.

【0003】従来の自動分注装置で行われる分注方法について説明する。 [0003] For dispensing method carried out in a conventional automatic dispensing device will be described. まず、XYZロボットによって支持されたノズルが試料を入れた試験管上部まで搬送され、その後、ノズル先端が試料内に侵入するまでノズルを降下させる。 First, is conveyed to the top of the test tube to a nozzle, which is supported by the XYZ robot The sample was placed, then the nozzle tip lowers the nozzle to penetrate into the sample. その状態でポンプを駆動しノズル内に所定量の試料を吸引する。 Aspirating a predetermined amount of the sample by driving the pump nozzle in this state. その吸引後、ノズルを上昇させ、分注を行うべき容器までノズルを移動させる。 After the suction, the nozzle is raised to move the nozzle to the container to perform dispensing. 容器上方にノズルが位置した状態で、ノズルを降下させて所定の降下位置でノズルを停止させ、ノズル内に含まれる試料の一部または全部を容器へ吐出する。 In a state where the container upwardly in the nozzle is located, by lowering the nozzle stops the nozzle at a predetermined lowered position, discharges a part or all of the sample contained in the nozzle into the container. 吐出後、ノズルを上昇させ、他の容器又は初期位置へノズルを移動させる。 After discharge, the nozzle is raised to move the nozzle to another container or the initial position.

【0004】ところで、ノズル先端が容器内試料に侵入しない位置で吐出した場合、容器への試料吐出後ノズル先端に、試料が球状になって残留することがある。 Meanwhile, when the nozzle tip is discharged at a position that does not penetrate into the container in the sample, the sample after discharge nozzle tip to the container, it may sample remains turned sphere. つまり、ノズル内壁に付着していた試料が降下してノズル先端外側に試料球が生じる。 That is, sample ball is generated in the nozzle distal outer descends sample adhering to the inner wall of the nozzle is. この最後の一滴は、液体試料の表面張力によるものであり、その最後の一滴がノズル先端に付いた状態でノズルの移動を行えば、移動中にいわゆるぼた落ちにより液状試料が飛散し、汚染を生じさせてしまう。 The last drop is of a liquid sample by surface tension, by performing the movement of the nozzle with its last drop is attached to the nozzle tip, the liquid sample is scattered by the so-called dripping during movement, pollution the would cause.

【0005】そこで従来においては、吐出位置を下げ、 [0005] Therefore, in the prior art it lowers the discharge position,
容器内試料にノズル先端が侵入するようにして試料の吐出を行っていた。 Nozzle tip container sample so as to penetrate had done the discharge of the sample. このようにノズル先端を容器内試料に侵入させて、ゆっくりと上昇させることにより、上述した最後の一滴が容器内の試料側へ吸い取られ、ノズル先端に最後の一滴が残ることが防止される。 Thus by entering the nozzle tip in a container within the sample, by raising slowly last drop described above is sucked into the sample side of the vessel, the last drop in the nozzle tip may remain is prevented.

【0006】 [0006]

【発明が解決しようとする課題】しかしながら、所定量ずつ複数の容器へ分注を行う場合には、容器相互間で汚染が生じるおそれがあり、このためノズル先端を容器内試料に触れさせずに分注を行う要請がある。 [SUMMARY OF THE INVENTION However, when performing a dispense to a plurality of containers by a predetermined amount, there is a possibility that contamination between vessels cross occurs, the order nozzle tip is not exposed in a container within the sample there is a request to perform dispensing. つまり、試料が吐出される容器内に予め試薬などを入れてある所へ試料の吐出を行う場合があり、容器相互間で試料の混合が生じることを防止しなければならない。 In other words, there is a case where the discharge of the sample to the place that is put in advance reagent and in a container in which the sample is discharged, must be prevented from mixing of the sample occurs between the container other.

【0007】ところが、上述したように、ノズル先端が容器内試料に触れないようにある程度の高さにノズルを位置して吐出を行えば、ノズル内に残存した試料によりノズル先端に試料球が残ることになり、ノズル移動中の試料飛散を防止することができない。 [0007] However, as described above, by performing discharge by positioning the nozzle at a certain height so that the nozzle tip does not touch the container within the sample, the sample ball remains in the nozzle tip by the sample remaining in the nozzle It will be unable to prevent the sample scattering in the nozzle movement.

【0008】本発明は、上記従来の課題に鑑みなされたものであり、その目的は、試料吐出後にノズル先端に残る試料球を飛散させることなくノズルを移動させることができる分注方法を提供することにある。 [0008] The present invention has been made in view of the above-mentioned conventional problems, and its object is to provide a dispensing method can move the nozzle without scattering the sample balls remaining in the nozzle tip after the sample discharge It lies in the fact.

【0009】 [0009]

【課題を解決するための手段】上記目的を達成するために、本発明は、ノズルによって吸引された試料を容器に吐出する工程の後に、少量の空気を吸引してノズル内先端にエア層を形成するエア層形成工程が設けられ、前記エア層をノズル内先端に形成した後、前記ノズルの移動を行うことを特徴とする。 To achieve the above object of the Invention The present invention, after the step of discharging the sample aspirated by the nozzle to the container, the air layer at the tip in the nozzle by sucking a small amount of air forming an air layer forming step is provided for, after the air layer is formed at the tip in the nozzle, and performs the movement of the nozzle.

【0010】 [0010]

【作用】上記構成によれば、試料吐出後に、少量の空気が吸引されノズル内先端にエア層が形成されるので、ノズル先端に一度生じた試料球はノズル内に吸引され、かつその試料とノズル先端開口との間にはエア層が存在するため、ノズルからのぼた落ちを防ぎ、汚染を防止することができる。 According to the above arrangement, after the sample discharge, since a small amount of air the air layer is formed in the nozzle in the tip is sucked, once resulting sample sphere nozzle tip is sucked into the nozzle, and its sample between the nozzle tip opening due to the presence of air layers, preventing dripping from the nozzle, it is possible to prevent contamination. したがって、ノズル先端を容器内試料に触れさせずに吐出を行っても試料球による問題を回避できる。 Therefore, even if the discharge is not exposed to the nozzle tip in a container within the sample can be avoided the problem of sample sphere.

【0011】 [0011]

【実施例】以下、本発明の好適な実施例を図面に基づいて説明する。 BRIEF DESCRIPTION OF THE PREFERRED EMBODIMENTS example in the drawings of the present invention. 図1には、本発明に係る分注方法の好適な実施例が示されている。 Figure 1 is a preferred embodiment of the dispensing method according to the present invention is shown.

【0012】この分注方法は、試料の分注を行う自動分注装置において採用されるものであり、図1には各工程が模式的に示されている。 [0012] The dispensing method is intended to be employed in automatic dispensing apparatus that performs dispensing of the sample, each process is shown schematically in Figure 1.

【0013】ノズル10によって試料12が所定量吸引され、その後、ノズル10は吐出を行うべき容器14の上方に移動され、その後ノズル10が降下する。 [0013] Sample 12 by the nozzles 10 is predetermined suction amount, then the nozzle 10 is moved above the container 14 to be subjected to discharge, then the nozzle 10 is lowered. なお、 It should be noted that,
その降下位置として所定のダウン位置が定められ、その位置は吐出後も容器内試料16がノズル先端に触れない高さである。 Predetermined down position is defined as a lowered position, its position is the height of post-discharge even vessel sample 16 does not touch the nozzle tip.

【0014】(A)に示すノズル降下工程が終了した後、図示されていないポンプが駆動され、ノズル10内の試料12が容器14へ吐出される。 [0014] (A) after the nozzle drop step is completed shown, is driven pump not shown, the sample 12 of the nozzle 10 is discharged into the container 14.

【0015】すると、(B)に示すように、ノズル10 [0015] Then, as shown in (B), the nozzle 10
の先端にノズル10内壁に残存した試料によって試料球12aが付着することになる。 Samples ball 12a is to be deposited by the tip to the sample remaining in the nozzle 10 inner wall of. もちろん、最後の一滴が重力によって落下する場合もある。 Of course, there is a case where the last drop to fall by gravity.

【0016】その後、(C)に示すようにノズル10が上昇され、あるいはそのダウン位置の状態で、ポンプが駆動され所定量の空気の吸引が行われる。 [0016] Then, the nozzle 10 as shown in (C) is raised, or in a state of its down position, the pump is driven suction a predetermined amount of air is performed. ここで、その所定量とは例えば40μlである。 Here, the the predetermined amount such as 40 [mu] l.

【0017】(D)にはエア層20が示されている。 [0017] (D) is shown the air layer 20 is. 図示されるように、吸引された試料12aの下方にエア層20が形成されキャップされているため、いわゆるぼた落ちが生じることはない。 As shown, since the air layer 20 below the aspirated sample 12a is formed cap, not so-called dripping occurs.

【0018】そして、エア層20が形成された後、 [0018] Then, after the air layer 20 is formed,
(E)に示すようにノズル10が初期位置へ戻される。 Nozzle 10 as shown in (E) is returned to the initial position.

【0019】なお、本実施例では、ノズル10内の試料12を、ノズル10内壁に存在するものを除いて全て吐出したが、所定量ずつ複数の容器へ分注を行う場合にもエア層20の形成は有効である。 [0019] In this embodiment, the sample 12 of the nozzle 10 has been all ejected except those present in the nozzle 10 inner wall, an air layer even in the case of performing the dispensing into a plurality of containers by a predetermined amount 20 formation of is effective. すなわち、ノズル10 That is, the nozzles 10
内の試料12のうちの一部を吐出した後であっても、試料球12aが付着する場合が多く、エア層20の形成によればそのような試料球をノズル10内に吸引してノズル10の移動中に生ずる試料の飛散を有効に防止できる。 Even after ejecting a part of the sample 12 of the inner, often sample ball 12a is attached, by sucking such samples spheres according to the formation of the air layer 20 to the nozzle 10 in the nozzle the scattering of a sample occurring during 10 movement can be effectively prevented.

【0020】 [0020]

【発明の効果】以上説明したように、本発明によれば、 As described in the foregoing, according to the present invention,
試料吐出後にノズル先端内部にエア層を形成したので、 Since the formation of the air layer in the nozzle tip inside the sample after discharge,
ノズル先端に付着する試料球の飛散による汚染を防止して、信頼性の高い分注を行うことができる。 To prevent contamination by scattering of the sample spheres adhering to the nozzle tip, it is possible to perform highly reliable dispensing.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

【図1】本発明に係る分注方法の具体的な工程を示す説明図である。 Is an explanatory view showing a specific process of dispensing method according to the invention; FIG.

【符号の説明】 DESCRIPTION OF SYMBOLS

10 ノズル 12 試料 14 容器 16 容器内試料 20 エア層 10 nozzles 12 sample 14 container 16 container sample 20 Air layer

Claims (1)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】 ノズルによって吸引された試料を容器に吐出する工程の後に、少量の空気を吸引してノズル内先端にエア層を形成するエア層形成工程が設けられ、前記エア層をノズル内先端に形成して前記ノズルの移動を行うことを特徴とする分注方法。 After 1. A step of discharging the sample aspirated by the nozzle to the container, is provided an air layer forming step of forming an air layer at the tip in the nozzle by sucking a small amount of air, the air layer in the nozzle dispensing method and performing the movement of the nozzle formed in the tip.
JP4067363A 1992-03-25 1992-03-25 Dispensing method Expired - Lifetime JP2537584B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4067363A JP2537584B2 (en) 1992-03-25 1992-03-25 Dispensing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4067363A JP2537584B2 (en) 1992-03-25 1992-03-25 Dispensing method

Publications (2)

Publication Number Publication Date
JPH05273218A true JPH05273218A (en) 1993-10-22
JP2537584B2 JP2537584B2 (en) 1996-09-25

Family

ID=13342859

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4067363A Expired - Lifetime JP2537584B2 (en) 1992-03-25 1992-03-25 Dispensing method

Country Status (1)

Country Link
JP (1) JP2537584B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011107089A (en) * 2009-11-20 2011-06-02 Hitachi High-Technologies Corp Liquid mixing method and dispensing apparatus
JP2011149721A (en) * 2010-01-19 2011-08-04 Hitachi Plant Technologies Ltd Reagent aliquot and dispensation method using reagent dispensation nozzle, and reagent aliquot and dispensation mechanism

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5320229A (en) * 1976-07-29 1978-02-24 Tachikawa Spring Co Structure for head rest of automotive seat
JPS5742935U (en) * 1980-08-25 1982-03-09
JPS5836355U (en) * 1981-08-31 1983-03-09
JPS5933211A (en) * 1982-08-20 1984-02-23 Sato Seiyaku Kk Oleaginous ointment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5320229A (en) * 1976-07-29 1978-02-24 Tachikawa Spring Co Structure for head rest of automotive seat
JPS5742935U (en) * 1980-08-25 1982-03-09
JPS5836355U (en) * 1981-08-31 1983-03-09
JPS5933211A (en) * 1982-08-20 1984-02-23 Sato Seiyaku Kk Oleaginous ointment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011107089A (en) * 2009-11-20 2011-06-02 Hitachi High-Technologies Corp Liquid mixing method and dispensing apparatus
JP2011149721A (en) * 2010-01-19 2011-08-04 Hitachi Plant Technologies Ltd Reagent aliquot and dispensation method using reagent dispensation nozzle, and reagent aliquot and dispensation mechanism

Also Published As

Publication number Publication date
JP2537584B2 (en) 1996-09-25

Similar Documents

Publication Publication Date Title
US4834944A (en) Automatic analytical apparatus
DE69635760T2 (en) Analyzer with disposable reaction vessels
US6991002B2 (en) Tamper evident syringe tip cap and automated method for preparing tamper-evident syringes
DE19534955C2 (en) Chemical analyzer not eintauchendem agitator
US4528158A (en) Automatic sampling system
EP1155742B1 (en) Liquid dispensing apparatus and method
US5312757A (en) Sample distributing method
US5525298A (en) Apparatus for taking liquid content for use in analysis out of container
US5827744A (en) Method and apparatus for cleaning a liquid dispensing probe
JP3328048B2 (en) The method of mixing liquids
US4452899A (en) Method for metering biological fluids
US6602474B1 (en) Multi-vessel container for testing fluids
US5163582A (en) Apparatus and method for aliquotting blood serum or blood plasma
JP5179350B2 (en) Nested closed tube sampling assembly
EP0785434B1 (en) Probe for aspirating containers with probe cleaning means
CA2103384C (en) Fluid probe washing apparatus and method
WO2010104072A1 (en) Analyzer and method for cleaning dispenser probe
EP0606480A1 (en) Method of pipetting of blood sample
JP2003520127A (en) Distribution of droplets of the brittle porous substrate
JPH11295323A (en) Automatic dispenser and its method
DE69737619T2 (en) Automatic analyzer washing device
CA1166038A (en) Sample feeding arrangement
JPH08122336A (en) Cartridge container
WO1991019181A1 (en) Method and apparatus for pipetting liquid from a sealed container
US5211310A (en) Apparatus and method for dispensing phases of blood

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090708

Year of fee payment: 13

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110708

Year of fee payment: 15

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110708

Year of fee payment: 15

FPAY Renewal fee payment (prs date is renewal date of database)

Year of fee payment: 16

Free format text: PAYMENT UNTIL: 20120708

EXPY Cancellation because of completion of term
FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120708

Year of fee payment: 16