JPH05146289A - New bacillus microorganism and its use - Google Patents

Abstract

PURPOSE:To provide the subject new microorganisms belonging to Bacillus licheniformis, capable of degrading aflatoxin, useful for mycota growth inhibitors for preventing agricultural crop disease control, fermentation promoters for mitigating odor generation for livestock feces, or livestock growers, etc. CONSTITUTION:The objective microorganisms containing, as active ingredients, microorganisms belonging to Bacillus licheniformis and capable of degrading aflatoxin [e.g. Bacillus licheniformis DB-9011 (FERM BP-3418)]. The microorganisms are mixed with entirely aged waste sawdust used for artificial Lyophyllum aggregatum culture, thus obtaining (A) mycota growth inhibitors for e.g. pathogenic mould, capable of agricultural crop disease control through application on soil or plants, (B) fermentation promoters capable of mitigating odor generation through application on e.g. livestock feces or addition to aerating tanks, or (C) livestock growers capable of promoting nutrient absorption through the digestive tracts, steadily increasing livestock weight and increasing egg production by administration to livestock.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel microorganism belonging to Bacillus licheniformis and its use.

[0002]

2. Description of the Related Art Various microbial activities are involved in a wide range of agricultural management, and there are cases where this relationship is beneficial and disadvantageous to agriculture.

For example, fungi are microorganisms usually called fungi, which are roughly divided into algae, ascomycetes, basidiomycetes, and incomplete fungi. Among them, yeasts are useful. There are many things.

[0004] However, for example, some filamentous fungi, etc., which exist in the soil and serve as disease-causing fungi of agricultural crops, it is important for agricultural management to prevent their growth.
In addition, although compost is useful as a fertilizer for agricultural products, it contains livestock manure and the like, which may cause offensive odor due to the growth of fungi and may become a hotbed for the growth of pathogenic fungi. was there. Further, infectious diseases of agricultural crops caused by fungi such as filamentous fungi are infectious and may completely destroy agricultural crops. Therefore, it is desired to develop means for effectively preventing the infection.

Conventionally, as methods for inhibiting the growth of fungi, methods using various antibacterial agents and bacteriostatic agents have been known, but in these methods, since the antibacterial agent and bacteriostatic agent components are decomposed, It has been necessary to be sprayed at regular intervals, and there are drawbacks that it inhibits the activities of other useful microorganisms and loses its effect due to the development of resistant bacteria, so it cannot be said to be sufficiently satisfactory.

[0006] Therefore, there has been a demand for the development of a fungal inhibitor which has a long-term action and has no problem of the development of resistant bacteria.

On the other hand, due to changes in living environment and consciousness,
The demand for the prevention of offensive odors and offensive odors is increasing,
For example, it is often the case that neighboring livestock farmers, etc., who have been continuing production, are required to prevent or reduce the offensive odor or offensive odor generated from livestock dung.

Masking method, chemical deodorizing method, physical deodorizing method, biological deodorizing method and the like have been known as deodorizing methods for preventing bad odors. In livestock production, where is continuously and in large quantities produced, there is a limit to the action of chemical deodorant methods and physical deodorant methods.
Furthermore, the masking method does not pose any problem. Therefore, a biological deodorizing method that quickly ferments livestock manure, which is a source of malodor, and prevents the generation of malodor is attracting attention.However, a fermentation promotion method that prevents malodor from livestock manure has been established. The current situation is that there are none. Also, there is a similar problem with kitchen waste that is discharged from old-fashioned toilets and households, but these have not been solved yet. Furthermore,
Lignin, which is one of the main components of woody plants such as wood, bamboo, and straw, and which is a reticulated polymer compound, is difficult to decompose and remains as garbage.

[0009] Therefore, there is a strong demand for the development of a method for effectively preventing the generation of malodors by rapidly fermenting the malodorous sources such as livestock dung, human feces, and garbage, and even lignin with a simple operation. It was

[0010] Furthermore, one of the major concerns of livestock farmers is how to keep livestock healthy and obtain excellent meat and eggs. In actual breeding, livestock consume sufficient feed. In many cases, they cannot be satisfactorily bred because they are not digested, their digestion is insufficient, or they become sick. For example, when a large amount of feed, which is easily fermented, is fed to ruminants such as cows at one time, rumen acidosis occurs temporarily, and the appetite of the cow is reduced. Then, such a phenomenon causes abnormal pH of rumen gastric juice, damage to the stomach wall, abnormal production of amines and other metabolites, abnormal heat of fermentation, etc., resulting in normal digestion and absorption. Inhibit. Further, bacteria other than flora may abnormally proliferate and may migrate into the blood to cause gastrointestinal tract diseases, liver disorders, musculoskeletal disorders, reproductive disorders, heat stroke, mastitis, dermatitis and the like.

Therefore, in order to raise livestock in a healthy manner and obtain more livestock products, there has been a strong demand for development of a technique for promoting digestion of livestock feed and fattening livestock.

[0012]

[Means for Solving the Problems] The present inventors isolated many microorganisms from soil and searched for their properties. As a result, they found a novel microorganism belonging to Bacillus licheniformis and capable of degrading aflatoxin. It was
And this microorganism inhibits the development of fungi, shows an excellent fungal development inhibitory effect and fermentation is promoted by using this microorganism, and also decomposes lignin effectively,
Furthermore, they have found that when this livestock is fed with livestock to livestock, abnormal fermentation in the digestive tract of livestock is suppressed and the growth of livestock is promoted.

That is, an object of the present invention is to provide a microorganism belonging to Bacillus licheniformis and having aflatoxin degrading property. Another object of the present invention is to provide a fungal growth inhibitor containing the above microorganism as an active ingredient. Further, another object of the present invention is to
It is intended to provide a fermentation accelerator containing the above-mentioned microorganism as an active ingredient. Still another object of the present invention is to
It is intended to provide a livestock fattening agent containing the above microorganism as an active ingredient.

The microorganism of the present invention belongs to Bacillus licheniformis and is an aflatoxin degrading microorganism, and any microorganism corresponding to these may be used.

An example of the properties of these microorganisms is shown below.

As an example of such a microorganism, Bacillus licheniformis DB9011 found by the present inventors
The microbiological properties are as follows.

Form: A bacillus having a cell width of 0.7 to 0.8 μm. Oval spores are present in the center and do not swell the cells. It is motile and forms R-type colonies.
It grows slightly under anaerobic conditions.

Growth state in each medium: (1) DHL agar medium No growth. (2) MacConkey agar medium No growth. (3) Mannitol salt medium Good growth. There is gloss. There are no wrinkles on the surface of the colony, and the color of the colony is yellow. (4) Normal agar medium Good growth. No luster. There are wrinkles on the surface of the colony, and the color of the colony is white. (5) Heart infusion agar medium Good growth. No luster. There are wrinkles on the surface of the colony, and the color of the colony is white. (6) Blood agar medium (10% sheep blood added) Good growth. No luster. There are wrinkles on the surface of the colony, and the color of the colony is white. (7) PDA medium Good growth. No luster. There are wrinkles on the surface of the colony, and the color of the colony is white.

Physiological properties: Gram reaction: + Gelatin test: Growing state; Full liquefaction gelatin liquefaction; + Litmus milk: Reaction; Acid state; Coagulation nitrate reduction: + Denitrification reaction: − MR test: − Indole formation : -Hydrogen sulfide production: -Citric acid utilization (Crystezene): + Urease: -Oxidase: + Catalase: + Growth range: pH; 4-9 temperature; 25-50 ° C OF test: Fermentation and gas evolution ( (Due to glucose decomposition)

Utilization of sugar ■ ⊃
Gas generation Acid generation L-arabinose- + D-xylose- + D-glucose ++ D-mannose- + D-fructose- + D-galactose- + maltose (maltose)-+ sucrose (sucrose)-+ lactose ( Lactose)-+ Trehalose- + D-Sorbit- + D-mannitol- + Inosit- + Glycerin- + Starch- +

Decomposition of esculin: + Utilization of malonic acid: -Decomposition of arginine: + Decarboxylation of lysine: + Decomposition of urea: + Decomposition of aflatoxin: + Decarboxylation of ornithine: -Coagulase: -Hemolytic property: + Sodium chloride Tolerance: less than 10% Potassium cyanide resistance: developable Lecithinase:-

The present inventors determined that Bacillus licheniformis DB9011 was a novel strain from the above properties,
On May 21, 1991, the Ministry of International Trade and Industry, Institute of Industrial Science and Technology, Institute of Microbial Engineering, Microtechnology Research Institute No. 3418 (FERM BP-34
18) deposited.

In order to prepare a fungal growth inhibitor using the above-mentioned microorganism of the present invention, this microorganism is grown in a nutrient source such as a plant organic substance, defatted rice bran, or bran, particularly an aged plant organic substance. After that, it may be used as it is or in the state of a water suspension obtained by filtering this. In the case of a water suspension, the amount of microorganisms per ml is preferably 10 ⁴ or more.

[0024] Examples of the plant organic material used for propagation include those which are completely aged of the waste sawdust used for artificial shimeji cultivation. When such a plant organic material is used, Bacillus licheniformis is used. Changes from a vegetative type to a proliferative type, and its action improves as the number of bacteria increases.

When the fungal growth inhibitor of the present invention is applied to soil, it depends on whether it is to prevent diseases of agricultural products whose fungi are pathogens or to eliminate already existing pathogenic fungi. Can be sprayed at about 10 ⁹ per 1 m ² .

On the other hand, when the fungal growth inhibitor of the present invention is applied to compost or the like, it may be sprayed evenly on its surface or sufficiently mixed with these. By doing so, Bacillus licheniformis, which is an active ingredient, proliferates in compost and the like, and it becomes possible to suppress the growth of fungi. In this case, the amount of the fungal growth inhibitor to be used can be generally determined based on the disappearance of the odor from the compost or the like.

Further, in order to prepare a fermentation accelerator using the microorganism of the present invention, the microorganism of the present invention is carried on the above-mentioned plant organic substance, defatted rice bran, excipients such as bran, particularly aged plant organic substance. It is preferable to prepare. In this case, the amount of microorganisms per 1 g of the excipient is preferably 2 × 10 ⁵ or more.

The fermentation accelerator of the present invention contains a malodor source such as
It should be sprinkled evenly on the surface of livestock manure such as chicken manure, pig manure, cow manure, human manure, and garbage, or mixed sufficiently with these. By doing so, fermentation is promoted, so that a bad odor can be prevented. This deodorant effect is seen from the first day of application and lasts for 15 days or longer. For example, when the amount of livestock manure, which is a source of offensive odor, is constant and the excreted livestock manure does not accumulate from the top, a deodorizing effect can be expected by spraying evenly on the surface once every several months.

The fermentation in the aeration tank can be promoted by introducing the fermentation accelerator of the present invention into the aeration tank, which promotes the fermentation in the aeration tank.

The amount of the fermentation accelerator of the present invention used cannot be unconditionally determined because it varies depending on the type, amount, conditions, etc. of the malodor source.
Although it needs to be determined experimentally, generally, an appropriate amount of use can be determined based on the disappearance of the odor from the malodorous source.

Further, in order to prepare a livestock fattening agent using the microorganism of the present invention, the present invention is blended as an essential component and other components necessary for the growth of livestock are blended as optional components. Examples of such optional ingredients include:
Examples include defatted rice bran, plant organic substances, bran, calcium sources such as fossil shells, beer lees, various vitamins, nutrients and the like. The amount of microorganisms in 1 g of the livestock fattening agent is preferably 1 × 10 ⁵ or more.

Preferred examples of the livestock fattening agent of the present invention include:
An example is a mixture of defatted rice bran and fossil shellfish in an appropriate ratio, for example, about 4: 1, and then 1 × 10 ⁵ or more Bacillus licheniformis added thereto. In such livestock fattening agents, Bacillus licheniformis changes from a vegetative type to a proliferative type, proliferates with organic substances as nutrients, and its action is improved as the number of bacteria increases.

The livestock fattening agent of the present invention may be added to livestock feed and fed to livestock. In some cases,
You may feed instead of feed. When administered together with feed, it is generally added in an amount of about 0.5 to 10%, preferably about 1 to 5% with respect to the feed.

The period of use of the livestock fattening agent of the present invention varies depending on the type of livestock to be administered, the growth period, the degree of fattening, etc., and therefore cannot be determined unconditionally, but it must be determined experimentally.

[0035]

Although the reason why the microorganism of the present invention is used for many purposes such as a fungal growth inhibitor, a fermentation accelerator, and a livestock fattening agent is not clear yet, it is a microorganism that exists in the environment and has a disadvantageous effect, for example, a disease source. It is considered to suppress the growth of microorganisms that produce growth inhibitory substances such as sex fungi, malodorous filamentous fungi, and aflatoxin, and promote normal fermentation of Bacillus microorganisms.

More specifically, the action of the fungal growth inhibitor is as follows.
Possessed by microorganisms belonging to Bacillus licheniformis
Competitive growth with fungi, lytic activity against fungi,
It is considered that the production of the growth-inhibiting substance is one of the reasons or that they act synergistically.

Further, the action of the fermentation accelerator inhibits the growth of filamentous fungus that breeds in livestock manure or the like possessed by microorganisms belonging to Bacillus licheniformis and produces a malodor,
Instead, it is thought to be based on the property that it proliferates in livestock manure and decomposes it.

Further, the action of the livestock fattening agent of the present invention inhibits the growth of harmful bacteria in the digestive tract of livestock having microorganisms belonging to Bacillus licheniformis, and in place of this, it grows in the livestock tract, It is considered to be based on the property of decomposing lignin contained in the lignin, which is considered to be difficult to decompose.

The above-mentioned action is completely unobtainable by other microorganisms including microorganisms of other species belonging to the genus Bacillus.

[0040]

INDUSTRIAL APPLICABILITY The microorganism of the present invention can be utilized extremely advantageously in the field of agriculture in which microbial activity is involved.

For example, when the fungal growth inhibitor of the present invention is used, the growth of fungi, in particular, pathogenic filamentous fungi can be suppressed. Therefore, when applied to soil or plants, the disease of agricultural crops can be suppressed. It becomes possible to prevent. Further, by applying this fungal growth inhibitor to compost or the like, it is possible to inhibit the growth of pathogenic filamentous fungi in the compost and prevent the generation of malodor due to the growth of filamentous fungi.

Further, when the fermentation accelerator using the microorganism of the present invention is sprayed on livestock manure or the like, the fermentation is greatly accelerated,
Fermentation, which took half a year to one year, is completed in 1-2 months,
Moreover, during that time, a bad odor hardly occurs. On the other hand, when the fermentation accelerator of the present invention is used in the aeration tank, the sewage treatment time is halved compared to the conventional one, the generation of malodor is reduced, and the BOD value and SS value after the treatment are also 1/30 of the conventional one. It decreases more than a degree. On the other hand, when the fermentation accelerator of the present invention is added to the sawdust used as a litter for livestock breeding (broilers, sawdust pigs, fattening calves), faecal treatment is simplified and no foul odor is generated and spread As a result of the continuous fermentation in the feed, the bedding is warm even in winter, and the occurrence of diseases such as diarrhea and pneumonia, which are conventionally caused by the cold stomach of livestock, is reduced.

Furthermore, when the livestock fattening agent using the microorganism of the present invention is administered to livestock, the state in the digestive tract of livestock can be kept normal and the absorption of nutrients from the digestive tract is promoted.
The weight of livestock can be steadily increased and, for example, the production of eggs can be increased, which is extremely advantageous in animal husbandry. As described above, the microorganism of the present invention is extremely advantageous in agricultural management.

[0044]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Test Example 1 A slant medium of PDA agar is prepared, and the following fungi are inoculated and cultured at 25 to 28 ° C. for 7 to 10 days (most fungi form spores during this period). The spores thus formed are suspended in a physiological saline solution or a phosphate buffer solution to which a surfactant is added so that the spores will be 1 × 10 ⁶ cells / ml, to give a spore suspension. This spore suspension was prepared separately P
Spread 0.1 ml per plate on a DA agar medium plate (90 mm diameter petri dish) and spread evenly with a Conra stick to allow the medium to fully absorb. 10 ⁶ /
1 spot of Bacillus suspension prepared to about 25 μm
1) Inoculated and cultured at 25 to 28 ° C. for 5 to 10 days, and formation of an inhibition circle was observed. As a result, Bacillus licheniformis DB-9011 inhibited the growth of all fungi.

[0046]

Example 1 A waste sawdust used for cultivation of fully matured artificial shimeji mushroom was prepared by adding 2 × 10 ⁵ pieces of Bacillus licheniformis DB-9011 (FERM BP-341) per 1 g of sawdust.
8) was added to prepare a fungal growth inhibitor (product 1 of the present invention).

Example 2 The product 1 of the present invention of Example 1 was stored for 10 days at about 20 ° C., 100 g of the product was suspended in 1000 ml of water, stirred sufficiently, and then filtered. This filtrate was used as a liquid fungal inhibitor (invention product 2). In 1 ml of the product 2 of the present invention,
1 × 10 ⁴ Bacillus licheniformis DB-9011 was contained.

Example 3 12 kg of the product of the present invention was sprinkled on a peach tree which had withered from a Monpa disease caused by a fungus, and after thoroughly mixing with soil,
Newly planted peach tree seedlings. Seven months after transplanting, the seedlings of this peach tree were well rooted and the growth of the tree was great. In addition, when a peach tree dies due to Monpa's disease, a large amount of pathogenic filamentous fungi exist in the soil, and the seedlings to be newly planted immediately die. It is said that it cannot be planted.

Example 4 In a cucumber field where wilt had occurred due to root-knot disease, 100 g of the product 1 of the present invention was sprayed per strain at several holes at the root. The dead seedlings also began to sprout one week after spraying, and the beard vines grew more and more and the tree vigor recovered.

Example 5 At an apple farm where rot (filamentous fungal disease) frequently occurs,
The effect of the fungal growth inhibitor of the present invention was examined. After cutting off the branches affected by rot, the present invention product 2 is used in a ratio of 10 liters per tree to all apple trees.
Was sprayed. As a result, no tree was infected with rot after spraying. In addition, the vigor of the trees already infected with the rot disease has recovered.

Example 6 The effect of the fungal growth inhibitor of the present invention was examined in a greenhouse where gray mold disease had occurred. Cucumber strains for which the generation of gray mold can be visually judged are pulled out, and then the product 2 of the present invention is 800 m per 1 m ^{2 in the} entire greenhouse.
It was sprinkled so that it would be l. As a result, there was no Botrytis cinerea in this greenhouse and the yield was not affected. If this disease occurs, the yield of cucumber will usually be greatly reduced.

Example 7 Regarding the antifungal agent of the present invention, Aspergillus flavus having aflatoxin-producing ability
Using NRRL 2999, its growth inhibitory effect and toxin production inhibitory effect were examined by the following method.

Preparation of antifungal agent: One platinum loop of Bacillus licheniformis DB 9011 was shake-cultured for 24 hours in 100 ml of Brain Heart Infusion,
A 10-fold diluted with water was used (antifungal agent 3).

Experimental Results: (1) Test in culture bottle Potato dextrose agar medium (PDA medium) was placed in a test culture bottle, and after solidifying, 1 g of corn flour and the test 10 ⁶ microorganisms were added.
Then, 1 drop (about 30 μl) of antifungal agent 3 was dropped into this, followed by culturing at about 30 ° C., and 4 days and 6 days after the start of culturing.
On the day, the growth of Aspergillus flavus and other fungi was examined. As a result, no growth of fungi including Aspergillus flavus was observed in the culture bottle to which the antifungal agent 3 had been dropped. On the other hand, antifungal agent 3
In the control in which was not added dropwise, Aspergillus flavus hyphal growth and conidia formation were observed, and further fungal growth was also observed.

(2) Test in Petri Dish On a PDA medium solidified in a petri dish, a test feed of 0.
After placing 5 g and inoculating 10 ⁶ test microorganisms, 1 drop (about 25 μl) of antifungal agent 3 was dropped by a pipette. Then, this was cultured at about 30 ° C., and the growth status of Aspergillus flavus and other fungi was observed on the 4th and 6th days after the start of the culture. The results are shown below.

[0057] * Formulated feed A Feed for adult chicken breeding ** Formulated feed B Feed for fattening pigs In addition, when the antifungal agent 3 is not added dropwise, mycelial growth and conidia formation of Aspergillus flavus were observed, and Fungal development was also noted.

(3) Inhibition of aflatoxin production After the culture of (1) was continued for 17 days from the start of the test, this culture was extracted twice with 50 ml of chloroform,
After concentrating to 2 ml, it was subjected to thin layer chromatography to examine the presence or absence of aflatoxin production. As a result, production of aflatoxins B ₁ and G ₁ was observed in the control, whereas production thereof was not observed in the case of using the antifungal agent 3.

Example 8 A fermentation accelerator was prepared by adding 2 × 10 ⁵ Bacillus licheniformis DB-9011 to 1 g of sawdust to scrap sawdust used for cultivation of fully matured artificial shimeji mushrooms. Invention 4).

Example 9 On the surface of 100 g of chicken feces, 4, 3 g of the product of the present invention obtained in Example 8 was evenly sprayed. As a result, 30 to 45 times of livestock manure is said to take 6 months to 1 year to ferment conventionally.
Fermented completely in the day. In addition, the generation of offensive odor was extremely small as compared with the case where the product of the present invention was not sprayed. Similar effects were observed when pig dung or cow dung was used.

Example 10 60 kg of the product 4 of the present invention was put into a 25-ton aeration tank for treating pig manure at a pig farm (scale of 1000 pigs). The water temperature in the aeration tank at the time of charging was 22 ° C., but the water temperature the next day had risen to 28 ° C., and steam that had not been generated before was being generated. In addition, the bad smell was considerably improved. Furthermore, the BOD value in the aeration tank before the product of the present invention is 800 mg / l, SS
The value was 25,000 mg / l, but one month after the injection, these became 16 mg / l and 20 mg / l, respectively.

Example 11 150 kg of the product 4 of the present invention was put into 5 tons of the aeration tank of a pig farm (6000 head scale). One day after the injection, the offensive odor in the test aeration tank was considerably reduced. In addition, it was confirmed that the fermentation was carried out by generating bubbles that were not observed in the bacteria used in the past.

Example 12 200 liters of the contents of 400 liters of a pump-out type external toilet (70% urine volume, 30% stool volume) were thoroughly stirred, and then 41 kg of the product of the present invention was added thereto and further stirred. It had a bad odor before it was put in, but after 4 days, it changed to the odor of a lump.
Further, after 7 days, the odor slightly felt in the toilet bowl, and after 10 days, the odor disappeared. After that, no odor was observed even one month later.

Example 13 [0064] After all the contents of 400 liters of the internal toilet with suction type were sucked out with a vacuum car, 1 kg of the product 4 of the present invention was evenly sprayed. Observation was carried out for one month immediately after spraying, but no odor was observed during that period.

Example 14 A week-old household garbage (very bad odor) is put in a shopping plastic bag, and 4 pieces of the product of the present invention 4 are put into the bag with one hand. The bad odor changed to a sweet and sour odor 3 days after the addition, and the raw vegetables were completely melted 1 week after the addition.

Example 15 100 g of the product 4 of the present invention was placed in a 15-liter household garbage container (containing 20%). 8 hours after the addition, the offensive odor disappeared, and there was no odor even when the amount was 60%.

Example 16 In 14 pig bunches of 16 pig farms (scale of 1500 heads), 1 ton of sawdust plus 3% of the product 4 of the present invention was used as a litter for 2 pig bunches. Laid to thickness. The next day, the odor disappeared in the pig bun, and the litter did not dry, so it kept a proper amount of water and was not dusted. In addition, the sawdust was fermented and kept at an appropriate temperature, so that diarrhea of the swine coming from the cold did not occur. From the 3rd week of the test, the color of the pig manure changed, and even the just excreted pig manure did not smell (probably because the pig in the test pig bun ate the product 4 of the present invention in the litter). On the other hand, in the control area where only sawdust was used as the bedding, a foul odor was generated and dust was present. It should be noted that the litter of the test pig bunches after shipment from the pigs did not have an offensive odor, was rapidly fermented by being accumulated, and could be used again as litter after the fermentation was completed.

Example 17 1 × 10 ⁵ Bacillus licheniformis DB-901 per 1 g was added to a mixture of defatted rice bran 80% and fossil shell 20%.
1 (FERMBP-3418) was added to prepare a livestock fattening agent (invention product 5).

Example 18 A test medium was prepared by adding 0.5 mM α-naphthol and 0.1% p-cresol to each potato agar medium. This test medium was inoculated with Bacillus licheniformis DB-9011 strain and cultured for 5 to 10 days. The test medium supplemented with α-naphthol was colored in purple and production of laccase was confirmed. Also, p
-The cresol-added test medium was colored brown and the secretion of tyrosinase was confirmed. From this result, it was confirmed that this strain has a lignin degrading ability.

Example 19 The product 5 of the present invention was added so as to be 3% to the feed, and 1
The cows were fed for months. As a result, when any of the animal fattening agents was added, the milk yield of dairy cows increased by about 5% as compared with the case where no animal fattening agent was added. Furthermore, problems such as reproductive disorders did not occur, and the effect was also recognized for their prevention.

Example 20 The effect of the livestock fattening agent of the present invention on chickens was investigated using 20 broiler chickens (Cobb breed) in 1 group. First, the present invention product 5
A test section in which 3% was added to the feed and a test section containing only the feed (control section) were set, and the animals were raised for 3 weeks, and the average body weight was measured. As a result, as for the body weight after breeding, the average weight of the chickens in the test group to which the livestock feed-improving agent of the present invention was administered was 150 g heavier than that of the control group. In addition, in the results of autopsy, no abnormalities in the intestinal tract were observed in all the chickens in the test group, whereas petechiae and bleeding in the duodenum and jejunum were observed in all 10 chickens in the control group. In addition, the length of the intestinal tract of chickens was about 1.5 times that of the control group compared to that of the control group, indicating that digestion and absorption became active. When the product 2 of the present invention was used, almost the same effect was obtained.

Example 21 The effect of improving the spawning rate by the administration of the livestock fattening agent of the present invention was investigated using 10 laying hens (Decalb TX chick; 143 day old) in 1 group. A test section (3%
Plots), test plots with 10% added (10% plots) and feed-only plots (control plots) were set up and raised for 2 months, and the egg production rate during that period was examined. The results are shown in Table 2. In addition, the diet was fed ad libitum and various vaccines were inoculated as usual.

[0073]

As is clear from these results, when the livestock fattening agent of the present invention was used, the egg laying rate of chickens was improved. The weight gain of the chickens during the test period and the autopsy results were the same in the 3 wards, but the feed intake was 25 g / day per bird in the 3% ward and about 2 g / day in the 10% ward compared to the control ward. There were many.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Internal reference number FI Technical display location C12N 1/20 E 7236-4B 1/38 7236-4B // (C12N 1/20 C12R 1:10 )

Claims (17)

[Claims] 1. A microorganism belonging to Bacillus licheniformis and capable of degrading aflatoxin. 2. A fungal growth inhibitor containing a microorganism belonging to Bacillus licheniformis and having aflatoxin degrading property as an active ingredient. 3. The fungal growth inhibitor according to claim 2, which suppresses the growth of pathogenic fungi derived from plants and animals. 4. The fungal growth inhibitor according to claim 2, which suppresses the growth of fungi having aflatoxin-producing ability. 5. The fungal growth inhibitor according to claim 4, which is applied to cereals or tree nuts. 6. The fungal growth inhibitor according to any one of claims 2 to 4, which further contains an aged vegetable organic substance as an active ingredient. 7. The fungal growth inhibitor according to any one of claims 2 to 4, which is in a liquid form. 8. A fermentation accelerator containing, as an active ingredient, a microorganism belonging to Bacillus licheniformis and having aflatoxin degrading properties. 9. A fermentation accelerator containing, as active ingredients, a microorganism belonging to Bacillus licheniformis and having aflatoxin degrading properties, and an aged plant organic substance. 10. The fermentation accelerator according to claim 8 or 9, which is applied to a malodor source for the purpose of deodorizing. 11. A livestock fattening agent which contains, as an active ingredient, a microorganism belonging to Bacillus licheniformis and having aflatoxin-degrading properties. 12. A method for controlling a crop disease caused by a pathogenic fungus, which comprises applying a liquid fungal growth inhibitor which belongs to Bacillus licheniformis and which contains an aflatoxin-degrading microorganism as an active ingredient to agricultural crops. 13. A method for preventing livestock diseases caused by a pathogenic fungus, which comprises adding a fungal growth inhibitor, which belongs to Bacillus licheniformis and which contains, as an active ingredient, an aflatoxin-degrading microorganism to livestock feed. 14. A method for preventing the production of aflatoxins in cereals or tree nuts, which comprises applying to the cereals or tree nuts a fungal growth inhibitor which belongs to Bacillus licheniformis and which contains, as an active ingredient, a microorganism having aflatoxin-degrading properties. 15. A method for promoting fermentation of an offensive odor source, which comprises applying a fermentation accelerator containing a microorganism belonging to Bacillus licheniformis and having an aflatoxin degrading property as an active ingredient to the offensive odor source. 16. The fermentation promoting method according to claim 15, wherein the malodor source is livestock dung, human dung, or garbage. 17. A method for fattening livestock, which comprises adding to the livestock feed a livestock fattening agent that contains, as an active ingredient, a microorganism belonging to Bacillus licheniformis and having aflatoxin-degrading properties, and feeding the livestock to livestock.