JPH04279861A - Biochemical automatic analyzer - Google Patents

Abstract

PURPOSE:To improve an analyzing capacity in a biochemical automatic analyzer. CONSTITUTION:A biochemical automatic analyzer dispenses a specimen stored in cassettes in a sampler into a reactor vessel by means of a pipet device so as to serve also as electrolyte analysis, and can carry out absorption, dispensing and cleaning simultaneously on reagents corresponding to measuring items from the first reagent table and the second reagent table by means of the pipet device consisting of three nozzles.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic biochemical analyzer, and particularly proposes an automatic analyzer that can greatly shorten processing time in biochemical analysis.

0002

[Prior Art] In an automatic analyzer that conducts analysis by transporting a plurality of reaction vessels, a sample, a first reagent,
As a method of dispensing the second reagent and sequentially performing optical measurements, for example, Japanese Patent Application No. 197930/1982
95358) was proposed.

0003

[Problems to be Solved by the Invention] Since the above-mentioned application as a prior art was mainly aimed at measuring biochemical immune reactions,
Since the processing speed is slow and the reagent containers can be used even if the number of reagents is small, the reagents were stored in one turret. However, in the present invention, the processing capacity is increased and the reagents are stored in the first reagent and the second reagent. It was used separately as a reagent.

0004

[Means for solving the problem] In the present invention, the first
The reagent and the second reagent are stored separately in separate reagent containers, and the first reagent pipette and the second reagent pipette each have three nozzles, and the suction, dispensing, and washing operations are alternately performed. We adopted a method to do so.

In a pipette device that simultaneously dispenses a test solution, a four-nozzle pipette is used, each of which uses a first pot to inhale the sample and a second pot to measure electrolytes, so a comparison electrode and a reference electrode are used. A method is incorporated in which the electrolyte concentration can be calculated from the potential difference between the measurement solution and the potential difference of the calibration solution stored in advance in the computer.The third pot dispenses the test solution into the reaction container, and the fourth pot You can wash pipettes alternately. In this way, by increasing the speed of the reaction turret and the speed of the pipette, high-speed processing of the automatic analyzer was made possible.

[0006]

EXAMPLE FIG. 1 shows an automatic biochemical analyzer according to the present invention. This automatic biochemical analyzer is equipped with a required number of sample cassettes 2 that hold a required number of containers 1 each containing a required amount of specimen, and the cassettes 2 are intermittently transported to a specimen collection position at a required timing. The sample in the container 1 can be read with a bar code, including its history and test items, and can be stored in a computer and can be accessed randomly.

[0007] A sample pipette device P for collecting a required amount of a specimen from a container 1 and a reaction container 3 into which a sample weighed with the same pipette is injected at a required position are arranged on a reaction table H at required intervals. .

As shown in FIG. 1, four pipette devices are held at predetermined intervals in a turret-shaped pipette holder, and are controlled to move intermittently in 90° increments by a motor (not shown) and a known cam device. . That is, each pipette is rotated at the P1 position to aspirate the required amount of the general sample, enters the electrolyte analysis pot that holds the reference electrode and the reference electrode at the P2 position, determines the potential difference of the measurement solution, and pre-injects the calibration solution. The potential table is stored in a computer (not shown), and the electrolyte concentration can be calculated from the difference. Further, at P3, a general or emergency sample is dispensed into the reaction tube 3, and at P4, washing is performed, and then the tube rotates again to the P1 position. Each pipette is equipped with a well-known suction pump and a discharge pump (not shown), and is set to operate when a suction cam and a discharge cam, whose rotations are controlled by a signal processing position, engage with each other.

The reaction tube 3 for dispensing the sample in this manner is disposed on the outer edge of the reaction table H, which is rotated intermittently by, for example, a Geneva mechanism drive device, and is rotated in the direction of the arrow. Dispensing one reagent, stirring (primary), dispensing a second reagent, stirring (secondary), photometry, and washing are performed in sequence, and the reaction tube 3 is rotated once and moved one pitch, and the operations are repeated in sequence.

[0010] The first reagent table 7 and the second reagent table contain the first reagent and the second reagent and are structured in a turret shape, and as shown in FIG. 2, the central part 14 has a fixed shaft 12. It shows a housing case 21 which is supported by a shaft and has a cylindrical inner side 21C and an outer plate 21a.
These have an annular shape, and each reagent can be selected using a bar code.The internal gear 22 and the external gear 24 mesh with each other, and can be rotated in forward and reverse directions by a drive device 38.
and a cooling/warming section 60.

In the room temperature storage section, a part of the storage case 21 is divided into a cooling storage section 60 by compartments 34a, 34b, and 34c, and in the room temperature storage section 55, a heating element 36 is fixed to the outer surface of the bottom plate 21b with a restraining material 37. , heat radiation is prevented by the heat insulating material 38, and electrical contacts 41a and 41b are provided, and electricity is supplied through the control circuit to maintain the room temperature.

The cooling storage section 60 has a gap 47 between the storage case 21 and the inner peripheral surface of the side wall 50a of the enclosure member 50, and the reagent bottle 3.
A cold air region 46 is formed from a cold air passage 70 sequentially formed by a gap 48 with the top wall 50 of 2 and the hollow part 14, and the cold air is communicated by the blowing means 43, and the thermoelectric element (Peltier element) 5
An electric cooling means 2a is provided as a cooling source, and an external heat sink 53a is attached to the base plate 53. This heat radiation is used to heat the washing water.

The pipette nozzle is provided with a heating means and a liquid level sensor for detecting the reagent liquid level. The main shaft 53 and the pipette nozzles 150, 151, 152 move up and down, and a bush 180 is provided at an intermediate position. Transmission takes place.

To move the main shaft 153 up and down, the vertical drive means consists of drive transmission elements including a pulse motor 156, which transmits power from rotary gears 158, 160, 162, 164 to the rack 168, and the rotation drive means is a pulse motor 170.
is transmitted to rotate member 76 by gear trains 172 and 176.

When the automatic analyzer starts operating the reagent pipetting devices 4 and 5, when the reaction tube 3 reaches the reagent discharge position, it rotates by reading the bar code and moves to the reagent suction position according to the measurement item. The pipette nozzle 50 is inserted into the reagent bottle at the reagent suction position using the vertical movement and rotation drive means, the downward movement is stopped by the action of the liquid level sensor, and the required amount is sucked by the reagent pump, and then the pipette nozzle 150
is raised, pulled out from the reagent bolt, sent to the reagent discharge position, inserted into the reaction tube 3 at that position, and extruded and dispensed by the reagent pump.Furthermore, in the next step, the pipette nozzle 150 cleans the cleaning trough W and discharges the cleaning liquid. Clean the inside and outside surfaces of the nozzle.

Now, when the pipette nozzle 150 is sucking the reagent, the nozzle 151 is dispensing the reagent into the reaction container 3, and the nozzle 152 is in the washing step W. This cycle is effective because it is repeated in three turns using the pipette nozzles 150, 151, and 152. The functions of the first reagent pipette 4 and the second reagent pipette 5 have similar time schedules.

The colorimetric detector 6 uses a multi-wavelength photometer and is composed of a light source lamp, a condensing lens, a reflecting mirror, a slit, a concave diffraction grating, a light receiving element, an AD exchanger, and a microcomputer.

When the reaction container 3 reaches the cleaning position, the cleaning section 9 uses a cleaning pump device to discharge and suction liquid into the reaction container 3 for cleaning, and discharges the waste liquid out of the system. The cleaning line utilizes heat radiated from the external radiator 53a of the thermoelectric element 53 and uses it as hot water. The cleaning method is carried out in eight stages, with alkaline cleaning in the second stage, acidic cleaning in the fourth stage, and pure water cleaning for the others.

[0019] Optical measurements are carried out with one rotation of the reaction table and one rotation of the reaction table.
The absorbance of each item of all the reaction tubes is measured every time the tube rotates in pitch increments, the time course of the reaction is obtained, and the highest measurement point of the measurement item is measured. After the measurement, the reaction vessel 3 is cleaned and reused.

The control device of this automatic biochemical analyzer includes a power supply section (not shown), a microcomputer, an operation panel,
The computer consists of a personal computer for data processing.The computer consists of a keyboard, CRT display, magnetic disk storage device, printer, etc.The storage device contains all the data necessary for clinical examinations, such as test items, patient information, and various statistical data. hold.

[0021]

[Effects of the Invention] As is clear from the above embodiments, the present invention is useful when performing automatic biochemical analysis using a large number of samples and various types of reagents in response to a large number of measurement items. The aim was to speed up notes and improve accuracy, and the results were recognized.

[Brief explanation of the drawing]

FIG. 1 is a plan view of an automatic biochemical analyzer based on an embodiment of the present invention.

FIG. 2 is a transparent diagram of an arrow in a reagent table.

FIG. 3 is a cross-sectional view of a reagent table.

FIG. 4 is a cross-sectional view of a reagent pipetting device.

[Explanation of symbols]

S Sampler H Reaction table 1 Sample container 2 Cassette 3 Reaction container 4 Reagent pipette 5 Reagent pipette 6 Colorimetric detector 7 Reagent table 8 Reagent table 9 Cleaning section P Pipette device W Washing trough

Claims (1)

[Claims] [Claim 1] A sample cassette containing a plurality of specimens, a pipette device for dispensing, measuring, and washing the specimens in the containers at predetermined positions, and reaction tubes held in a turret shape at predetermined intervals. A feeding device, a driving device that rotates the feeding device intermittently, a first reagent that alternately dispenses, aspirates, and washes the reaction tube dispensed at the sample dispensing position, a means for dispensing, and a second reagent. An automatic biochemical analyzer characterized by comprising a dispensing means and an optical measurement.