JPH04148649A - Production of water-soluble shell membrane - Google Patents
Production of water-soluble shell membraneInfo
- Publication number
- JPH04148649A JPH04148649A JP2271560A JP27156090A JPH04148649A JP H04148649 A JPH04148649 A JP H04148649A JP 2271560 A JP2271560 A JP 2271560A JP 27156090 A JP27156090 A JP 27156090A JP H04148649 A JPH04148649 A JP H04148649A
- Authority
- JP
- Japan
- Prior art keywords
- water
- eggshell
- decomposition product
- soluble
- eggshell membranes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 16
- 210000000998 shell membrane Anatomy 0.000 title abstract 3
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 46
- 108091005804 Peptidases Proteins 0.000 claims abstract description 22
- 102000035195 Peptidases Human genes 0.000 claims abstract description 16
- 241000271566 Aves Species 0.000 claims abstract description 5
- 210000003278 egg shell Anatomy 0.000 claims description 44
- 102000002322 Egg Proteins Human genes 0.000 claims description 40
- 108010000912 Egg Proteins Proteins 0.000 claims description 40
- 239000012528 membrane Substances 0.000 claims description 38
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 6
- 239000004365 Protease Substances 0.000 abstract description 12
- 239000002537 cosmetic Substances 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 7
- 241000272525 Anas platyrhynchos Species 0.000 abstract description 3
- 108010019160 Pancreatin Proteins 0.000 abstract description 3
- 229940055695 pancreatin Drugs 0.000 abstract description 3
- 241000287828 Gallus gallus Species 0.000 abstract description 2
- 108090000526 Papain Proteins 0.000 abstract description 2
- 241000286209 Phasianidae Species 0.000 abstract description 2
- 229940055729 papain Drugs 0.000 abstract description 2
- 235000019834 papain Nutrition 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Diphosphoinositol tetrakisphosphate Chemical compound OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 abstract 2
- 235000019833 protease Nutrition 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 32
- 229940012466 egg shell membrane Drugs 0.000 description 20
- 239000000463 material Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 229910052739 hydrogen Inorganic materials 0.000 description 7
- 239000001257 hydrogen Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 6
- 235000013361 beverage Nutrition 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 150000002431 hydrogen Chemical class 0.000 description 6
- 235000019419 proteases Nutrition 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 5
- 230000003020 moisturizing effect Effects 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241000228212 Aspergillus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 206010013911 Dysgeusia Diseases 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- MJBPUQUGJNAPAZ-AWEZNQCLSA-N butin Chemical compound C1([C@@H]2CC(=O)C3=CC=C(C=C3O2)O)=CC=C(O)C(O)=C1 MJBPUQUGJNAPAZ-AWEZNQCLSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000003676 hair preparation Substances 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- -1 promelain Proteins 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- MJBPUQUGJNAPAZ-UHFFFAOYSA-N Butine Natural products O1C2=CC(O)=CC=C2C(=O)CC1C1=CC=C(O)C(O)=C1 MJBPUQUGJNAPAZ-UHFFFAOYSA-N 0.000 description 1
- 108090000746 Chymosin Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000187392 Streptomyces griseus Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は水溶性卵殻膜の製造法に関する。詳しくは鳥類
の卵殻膜を一種以上の蛋白分解酵素で処理し、生成する
水溶性分解生成物を採取することを特徴とする水溶性卵
殻膜の製造法に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for producing water-soluble eggshell membranes. More specifically, the present invention relates to a method for producing water-soluble eggshell membranes, which comprises treating avian eggshell membranes with one or more proteolytic enzymes and collecting the resulting water-soluble decomposition products.
本発明は、鳥類例えば家禽類の卵殻の内面を覆っている
皮膜状の卵殻膜を、蛋白分解酵素で処理し、可溶化され
た水溶性卵殻膜を採取し、食品、化粧品医薬品などに応
用する場合に有用なものである。The present invention involves treating the film-like eggshell membrane that covers the inner surface of the eggshell of birds, such as poultry, with proteolytic enzymes, collecting the solubilized water-soluble eggshell membrane, and applying it to foods, cosmetics, pharmaceuticals, etc. This is useful in some cases.
従来、卵殻膜は、火傷や擦り傷などの患部に塗布すると
、消炎および上皮形成促進作用などの効果が知られてお
り、昔からその有用性が注目されていたにもかかわらず
、現在、飼料以外はほとんど利用さ、れていない、その
ため、卵殻膜の水溶性分解生成物を中心にその利用方法
について皮膚化粧料（特許公報昭５６−１１６８２）、
養毛ないし生毛化粧料（公開特許公報昭４８−４０９４
４）、飲食物もしくは嗜好物（特許公報昭５５−４５１
７６）、鮮度保持剤（公開特許公報子１−２３１８８１
）など種々提案きれているが、実際に応用には至ってい
ない、また例えば卵殻膜を無機酸及びアルカリによって
加水分解して水溶性卵殻膜を得る方法（特許公報昭５４
−２３９７５）。Conventionally, eggshell membrane has been known to have anti-inflammatory and epithelial formation promoting effects when applied to affected areas such as burns and abrasions, and although its usefulness has long attracted attention, it is currently being used as a non-feed product. Therefore, methods for using water-soluble decomposition products of eggshell membranes in skin cosmetics (Japanese Patent Publication No. 11682/1982) have been published.
Hair nourishing or natural hair cosmetics (Publication of Patent Publication No. 48-4094)
4) Foods, drinks, or luxury foods (Patent Publication 1984-451)
76), Freshness Preservation Agent (Publication Patent Publication No. 1-231881
) have been proposed, but none have been put to practical use.
-23975).
卵殻膜をアルカリ性含本有機溶媒中で分解処理して水溶
性卵殻膜を得る方法（公開特許公報子１−２７５５１２
）が知られているが、これらの方法によって得られる水
溶性卵殻膜は赤黒褐色に着色し、我慢のならない硫化水
素様の異臭をともなっており実用的ではなく、更にその
製造工程中に硫化水素が発生するなどの問題があるため
、上記の卵殻膜有効利用の妨げとなっている。Method for obtaining water-soluble eggshell membranes by decomposing eggshell membranes in an alkaline-containing organic solvent (Publication Patent Publication No. 1-275512)
), but the water-soluble eggshell membranes obtained by these methods are colored reddish-black and have an intolerable hydrogen sulfide-like odor, making them impractical, and furthermore, hydrogen sulfide is released during the manufacturing process. However, there are problems such as the occurrence of water, which hinders the effective use of eggshell membranes as described above.
本発明は、卵殻膜の有効利用を目的とし、卵殻膜の水溶
性分解生成物の製造工程中に硫化水素の発生する問題が
なく、しかも着色が少なく、異臭の少ない水溶性卵殻膜
を得るための製造方法を提供するものである。The purpose of the present invention is to effectively utilize eggshell membranes, and to obtain water-soluble eggshell membranes that do not have the problem of generating hydrogen sulfide during the production process of water-soluble decomposition products of eggshell membranes, have little coloring, and have little off-odor. The present invention provides a method for manufacturing.
本発明者らは、鳥類の卵殻膜から製造中に硫化水素を発
生せず、淡黄色で異臭の少ない水溶性卵殻膜を得るため
の製造法を開発する目的で鋭意研究を行った結果、卵殻
膜を一種以上の蛋白分解酵素で処理し、水溶性にする方
法を発明した。すなわち本発明は卵殻膜を水溶液に分散
させ、一種以上の蛋白分解酵素を添加して処理した後、
酵素を失活させ、不溶物を除き水に透明に溶解する水溶
性卵殻膜の製造法である。The present inventors conducted extensive research with the aim of developing a manufacturing method for obtaining water-soluble eggshell membranes that do not generate hydrogen sulfide during production from avian eggshell membranes, are pale yellow in color, and have little off-odor. We have invented a method of treating membranes with one or more proteolytic enzymes to make them water-soluble. That is, in the present invention, eggshell membranes are dispersed in an aqueous solution, treated with one or more proteolytic enzymes, and then
This is a method for producing water-soluble eggshell membranes that inactivate enzymes, remove insoluble matter, and dissolve transparently in water.
本発明の卵殻膜は、鳥類の卵殻膜であり、特に多量に入
手しゃすい家禽類例えば鶏、うずら、家鴨、あひる、七
面鳥などの卵の卵白、卵黄を除いた卵殻つきの卵殻膜で
あり、更に望ましくは卵殻を除去した卵殻膜である。卵
殻膜のみを集めるためには、人手によることが可能であ
るが、工業的方法ではないため、割卵機によって卵白、
卵黄を除いた卵殻の着いた卵殻膜を原料にすることがで
きる。このようにして集められた卵殻着きの卵殻膜はこ
のままの状態で使用してもよく、更に乾燥させ粉砕して
使用してもよく、また乾燥粉砕した卵殻着き卵殻膜から
卵殻膜を分離して使用することもできる。The eggshell membrane of the present invention is an eggshell membrane of a bird, in particular an eggshell membrane with eggshell from eggs of poultry that is easily available in large quantities, such as chicken, quail, domestic duck, duck, turkey, etc., with the albumen and yolk removed; Desirably, it is an eggshell membrane from which the eggshell has been removed. It is possible to collect only eggshell membranes manually, but since this is not an industrial method, egg whites,
Eggshell membranes, which have eggshells with egg yolks removed, can be used as a raw material. The eggshell membranes with eggshells collected in this manner may be used as they are, or may be used after being dried and crushed, or the eggshell membranes may be separated from the dried and crushed eggshell membranes with eggshells attached. You can also use
本発明に用いられる蛋白分解酵素は、パパイン、ブイチ
ン、プロメラインなどの植物起源の蛋白分解酵素、パン
クレアチン、レンニン、トリプシン、キモトリプシン、
ペプシンなどの動物起源の蛋白分解物酵素、 Ａｓｐｅ
ｒｇｉｌｌｕｓ ｏｒｙｚａｅ 、 Ａｓｐｅｒｇｉｌ
ｌｕｓ ｎｉｇｅｒ 、 Ａｓｐｅｒｇｉｌｌｕｓ ｍ
ｅｌｌｅｕｓ 、 Ａｓｐｅｒｇｉｌｌｕｓａｏｊａｅ
、 Ａｓｐｅｒｇｉｌｌｕｓ ５ａｉｔｏｉ 、 Ｂ
ａｃｉｌｌｕｓ ５ｕｂｔｉｌｉｓ、 Ｂａｃｉｌｌｕ
ｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ 、 Ｂａｃｉｌｌｕ
ｓ ｔｈｅｒｍｏｐｒ。The proteases used in the present invention include plant-derived proteases such as papain, butin, promelain, pancreatin, rennin, trypsin, chymotrypsin,
Proteolytic enzymes of animal origin such as pepsin, Aspe
rgillus oryzae, Aspergil
lus niger, Aspergillus m.
elleus , Aspergillus aojae
, Aspergillus 5aitoi , B
acillus 5ubtilis, Bacillus
s licheniformis, Bacillus
s thermopr.
ｔｅｏｌｙｔｉ 、 Ｒｈ１ｚｏｐｕｓ ｎ１ｖｅｕｓ
、 Ｒｈ１ｚｏｐｕｓ ｇｅｎｕｓ 、 Ｓｔｒｅｐ
ｔｏｍｙｃｅｓ ｇｒｉｓｅｕｓ 、 Ｐｅｎｉｃｉｌ
ｌｉｕｍ ｄｕｐｏｎｔｉ由来プロテアーゼなどの微生
物起源の蛋白分解酵素などがあげられる。これらの蛋白
分解酵素は単独であるいは２つ以上組み合わせて用いて
も良い。theolyti, Rh1zopus n1veus
, Rh1zopus genus , Strep
tomyces griseus, Penicil
Examples include proteases derived from microorganisms such as protease derived from Lium duponti. These proteases may be used alone or in combination of two or more.
本発明に用いられる蛋白分解酵素の使用量はその力価（
単位）、処理温度、処理時間、ｐＨ等によって相異する
が通常卵殻膜に対して０．５〜２０重量％添加して用い
られる。蛋白分解酵素が０．５重量％以下では卵殻膜の
分解が起こりにくく、また得られる卵殻の水溶性分解生
成物の収量が低くなる。蛋白分解酵素が２０重量％以上
では、卵殻膜の分解は問題なく起こるが、得られる卵殻
膜の水溶性分解生成物の収量は、はとんど同じになるの
に対し、経済的には高価な問題がある。The amount of protease used in the present invention is determined by its titer (
Although it varies depending on the unit), treatment temperature, treatment time, pH, etc., it is usually added in an amount of 0.5 to 20% by weight based on the eggshell membrane. When the amount of protease is less than 0.5% by weight, decomposition of eggshell membranes is difficult to occur, and the yield of water-soluble decomposition products of eggshells obtained is low. When the proteolytic enzyme is 20% by weight or more, the decomposition of eggshell membranes occurs without problems, but the yield of water-soluble decomposition products of eggshell membranes obtained is almost the same, but it is economically expensive. There is a problem.
本発明の蛋白分解酵素による処理温度は、２５〜６５℃
の温度範囲が望ましく、処理温度２５°Ｃ以下では、酵
素の活性が低くなり卵殻膜の分解に長時間必要になる問
題がある。また処理温度６５°Ｃ以上では酵素の失活が
激しく、卵殻膜の分解反応を制御することが困難であり
、得られる水溶性分解生成物が着色する問題がある。The treatment temperature with the protease of the present invention is 25 to 65°C.
A temperature range of 25° C. or less is desirable, and if the treatment temperature is below 25° C., there is a problem that the activity of the enzyme decreases and a long time is required to decompose the eggshell membrane. Furthermore, if the treatment temperature is 65° C. or higher, the enzyme is severely deactivated, making it difficult to control the decomposition reaction of eggshell membranes, and there is a problem that the resulting water-soluble decomposition products are colored.
未発明の卵殻膜の蛋白分解酵素処理によって得られる水
溶性分解生成物は、用いた蛋白分解酵素を加熱等により
変性、凝固させた後、炉遇して除去する。必要ならば、
脱臭、脱色の目的で活性度活性白土などの吸着剤を用い
ても良い、得られた水溶性分解生成物は、そのまま水溶
液の形で、あるいはスプレードライや凍結乾燥などの方
法で粉末化し、粉末状の形で製品化することができる。The water-soluble decomposition products obtained by the uninvented protease treatment of eggshell membranes are removed by denaturing and coagulating the proteolytic enzyme used by heating or the like, and then heating it in an oven. If necessary,
For the purpose of deodorizing and decolorizing, an adsorbent such as activated clay may be used.The obtained water-soluble decomposition product may be directly in the form of an aqueous solution, or it may be pulverized by a method such as spray drying or freeze drying. It can be commercialized in the form of
本発明の蛋白分解酵素処理による卵殻膜水溶性分解生成
物は、化粧液、クリーム、パック、ローション、化粧石
鹸などに配合して優れた皮膚化粧料を得ることができ、
メーキ〜ツブ化粧料９毛髪化粧料などにも幅広く利用で
きる。The eggshell membrane water-soluble decomposition product obtained by the proteolytic enzyme treatment of the present invention can be incorporated into cosmetic liquids, creams, packs, lotions, cosmetic soaps, etc. to obtain excellent skin cosmetics.
Makeup ~ Tubu Cosmetics 9 It can also be widely used in hair cosmetics, etc.
また本発明の卵殻膜水溶性分解生成物は、水に透明に溶
解するため飲料に用いることができる。Furthermore, the eggshell membrane water-soluble decomposition product of the present invention can be used in beverages because it dissolves transparently in water.
更に酵素で分解し低分子化しているため、低アレルギー
性であり、アレルギー症の人々に、麺類、パン類、菓子
類、嗜好品などの食品の形態で提供することもできる。Furthermore, since it is decomposed by enzymes and reduced to a low molecular weight, it is hypoallergenic and can be provided to people with allergies in the form of foods such as noodles, breads, sweets, and luxury items.
また家畜、家禽、養殖魚などにそのまま、あるいは飼料
と配合して蛋白質の吸収を促進させて成長を早める目的
にも使用することができる。It can also be used for livestock, poultry, farmed fish, etc., either as is or in combination with feed to promote protein absorption and accelerate growth.
本発明の卵殻膜水溶性分解生成物の製造に用いられる蛋
白分解酵素は、卵殻膜を構成している不溶性蛋白質を加
水分解し低分子の水溶性蛋白質に変化諮せる工程におい
て、低温で穏やかに分解反応を起こさせるため反応中に
着色物や異臭物の生成する副反応が起こらず、得られる
生成物は、着色が少なくまた硫黄水素臭などの異臭も生
成しない特徴がある。The proteolytic enzyme used in the production of the eggshell membrane water-soluble decomposition product of the present invention is used in the process of hydrolyzing insoluble proteins constituting eggshell membranes and converting them into low-molecular water-soluble proteins at low temperatures. Because the decomposition reaction occurs, side reactions that produce colored substances and foreign odor substances do not occur during the reaction, and the resulting product is characterized by having little color and not producing any foreign odor such as sulfur-hydrogen odor.
更に、本発明の卵殻膜水溶性分解生成物は、卵殻膜が本
来持っている消炎効果、上皮形成促進作用ヲ残しており
、コジワ、シミ、ソバカスなどの防止に役立つことが考
えられる。Furthermore, the water-soluble decomposition product of eggshell membranes of the present invention retains the anti-inflammatory effect and epithelial formation promoting effect inherent to eggshell membranes, and is thought to be useful for preventing fine lines, spots, freckles, etc.
次に本発明を実施例によって説明するが、本発明は、こ
の実施例の範囲に限定されるものではない。Next, the present invention will be explained by examples, but the present invention is not limited to the scope of these examples.
実施例１゜
乾燥した卵殻膜１００ｇを水１，０００ｍ１にいれて分
散させた。この卵殻膜の分散水溶液にコロラーゼ（樋口
商会株式会社製）１５ｇを添加し、撹拌しながら５０℃
で２００時間反応せた０反応後、９５℃で３０分間加熱
処理を行った後、炉遇して不溶物を除去し黄色の透明な
卵殻膜の分解生成物溶液を得た。Example 1 100 g of dried eggshell membranes were dispersed in 1,000 ml of water. 15 g of corolase (manufactured by Higuchi Shokai Co., Ltd.) was added to this aqueous dispersion solution of eggshell membranes, and heated to 50°C while stirring.
After 200 hours of reaction, heat treatment was performed at 95° C. for 30 minutes, and insoluble matter was removed by heating to obtain a yellow transparent eggshell membrane decomposition product solution.
この分解生成物溶液に活性炭５ｇを添加し、５０°Ｃで
３０分間脱色処理して、淡黄色の透明な液体を得た。こ
の分解生成物溶液の固形物含有量は約９０ｇであった。5 g of activated carbon was added to this decomposition product solution and decolorized at 50°C for 30 minutes to obtain a pale yellow transparent liquid. The solids content of this decomposition product solution was approximately 90 g.
この製造工程中およびこの分解生成物溶液は、硫化水素
様の異臭はほとんどなかった。またこの分解生成物溶液
を皮膚に塗ってみると保湿効果が認められた。更にこの
分解生成物溶液を試飲してみたところ、苦みが少し感じ
られた程度であり、飲料素材として十分使用できるもの
であった。During this manufacturing process and this decomposition product solution, there was almost no hydrogen sulfide-like off-odor. Furthermore, when this decomposition product solution was applied to the skin, a moisturizing effect was observed. Furthermore, when I sampled this decomposition product solution, I could feel only a slight bitterness, and it was sufficient to be used as a beverage material.
実施例２゜
乾燥した卵殻膜１００ｇを水１．０００ｍｊ！にいれて
分散させた。この卵殻膜の分散水溶液にスミチーム（新
日本化学工業株式会社製）１０ｇとパンクレアチン（ミ
クニ化学株式会社製）０．５ｇを添加し撹拌しながら５
０°Ｃで１８時間反応させた０反応後、９５°Ｃで３０
時間加熱処理を行った後、濾過して不溶物を除去し、黄
色の透明な卵殻膜の分解生成物溶液を得た。この分解生
成物溶液に活性炭１０ｇを添加し、５０°Ｃで３０分間
脱色処理して、淡黄色の透明な液体を得た。この分解生
成物溶液の固形物含有量は約８５ｇであった。Example 2゜100g of dried eggshell membrane and 1.000mj of water! and dispersed it. Add 10 g of Sumiteem (manufactured by Shin Nippon Chemical Co., Ltd.) and 0.5 g of pancreatin (manufactured by Mikuni Chemical Co., Ltd.) to this aqueous dispersion solution of eggshell membranes, and add 5 g of it while stirring.
After 18 hours of reaction at 0°C, 30 min at 95°C.
After heat treatment for a period of time, insoluble materials were removed by filtration to obtain a yellow transparent eggshell membrane decomposition product solution. 10 g of activated carbon was added to this decomposition product solution and decolorized at 50°C for 30 minutes to obtain a pale yellow transparent liquid. The solids content of this decomposition product solution was approximately 85 g.
この製造工程中およびこの分解生成物溶液は、硫化水素
様の異臭はほとんどなかった。またこの分解生成物溶液
を塗ってみると保湿効果が認められた。更にこの分解生
成物溶液を試飲してみたところ、苦みが少し感じられた
が飲料素材として十分使用できるものであった。During this manufacturing process and this decomposition product solution, there was almost no hydrogen sulfide-like off-odor. Also, when this decomposition product solution was applied, a moisturizing effect was observed. Furthermore, when I sampled this decomposition product solution, I found it to be a little bitter, but it was sufficient to be used as a beverage material.
実施例３゜
実施例１と全く同様に処理して卵殻膜の水溶性分解生成
物を得た。この溶液をエバポレーターで６００ｍｊ！ま
で濃縮しスプレードライして卵殻膜の酵素分解生成物の
粉末を約５０ｇを得た。Example 3 A water-soluble decomposition product of eggshell membranes was obtained in exactly the same manner as in Example 1. This solution is heated to 600mj using an evaporator! The mixture was concentrated and spray-dried to obtain about 50 g of powder of the enzymatic decomposition product of eggshell membranes.
この得られた卵殻膜の酵素分解生成物の粉末１ｇを水１
０ｍ ｌに溶解させると透明に溶解し、淡黄色の液体が
得られ、異臭もほとんど感じられなかった。またこの溶
液を皮膚に塗ってみると保湿効果が認められた。更にこ
の溶液を試飲してみたところ、苦みが少し感じられたが
飲料素材として十分に使用できるものであった。1 g of powder of the obtained enzymatic decomposition product of eggshell membranes was added to 1 g of water.
When dissolved in 0ml, a transparent, pale yellow liquid was obtained with almost no off-odor. When this solution was applied to the skin, a moisturizing effect was observed. When I sampled this solution, I found it to be a little bitter, but it was sufficient to be used as a beverage material.
比較例１゜
乾燥した卵殻膜１００ｇを２０％硫酸溶液１．０００ｍ
ｊ！にいれて分散させ、撹拌しながら７０℃で５時間反
応きせたところ褐色の液体を得た０反応終了後、羨酸石
灰約３００ｇを加えて中和し、生成した硫酸カルシウム
を濾過して不溶物を除去し、褐色の透明な卵殻膜の分散
生成物溶液を得た。この分解生成物溶液に活性１２０ｇ
を添加し、５０°Ｃで３０分間脱色処理して、褐色の透
明な液体を得た。この分解生成物溶液の固形物含有量は
約６５ｇであった。Comparative Example 1゜100g of dried eggshell membrane was added to 1.000ml of 20% sulfuric acid solution.
j! After the reaction was completed, approximately 300 g of lime envy was added to neutralize the resulting calcium sulfate, which was then filtered to remove the insoluble calcium sulfate. The material was removed to obtain a brown transparent eggshell membrane dispersion product solution. 120g active in this decomposition product solution
was added and decolorized at 50°C for 30 minutes to obtain a brown transparent liquid. The solids content of this decomposition product solution was approximately 65 g.
この製造工程中およびこの分解生成物溶液は、硫化水素
様の異臭が発生した。During this manufacturing process and this decomposition product solution, a hydrogen sulfide-like odor was generated.
この分解生成物溶液を皮膚に塗ってみると保湿効果が認
められたが、硫化水素臭が強く不快であった。更にこの
分解生成物溶液を試飲してみたところ、異臭が強く、ま
た味も悪く飲料素材として不適当であった。When this decomposition product solution was applied to the skin, a moisturizing effect was observed, but the hydrogen sulfide odor was strong and unpleasant. Furthermore, when I sampled this decomposition product solution, it had a strong off-odor and bad taste, making it unsuitable as a beverage material.
比較例２゜
乾燥した卵殻膜１００ｇを５％苛性ソーダ溶液１゜００
０ｍｊ！にいれて、撹拌しながら７０℃で１時間反応許
せたところ褐色の液体を得た１反応終了後、５％塩酸溶
液でｐＨ＝６．５に中和し、濾過して不溶物を除去し、
褐色の透明な卵殻膜の分解生成物溶液を得た。この分解
生成物溶液に活性１２０ｇを添加し、５０℃で３０分間
脱色処理して、褐色の透明な液体を得た。この分解生成
物溶液の固形物含有量は約７５ｇであった。Comparative Example 2 100g of dried eggshell membrane was mixed with 5% caustic soda solution 1°00
0mj! When the reaction was allowed to proceed for 1 hour at 70°C with stirring, a brown liquid was obtained.After the first reaction, the mixture was neutralized to pH=6.5 with 5% hydrochloric acid solution and filtered to remove insoluble materials. ,
A transparent brown eggshell membrane decomposition product solution was obtained. 120 g of the active substance was added to this decomposition product solution and decolorized at 50° C. for 30 minutes to obtain a brown transparent liquid. The solids content of this decomposition product solution was approximately 75 g.
この製造工程中、特に５％塩酸中和工程において硫化水
素様の異臭が激しく発生した。またこの分解生成物溶液
は、硫化水素様の異臭を発生していた。During this manufacturing process, a strong hydrogen sulfide-like odor was generated, especially in the 5% hydrochloric acid neutralization process. Additionally, this decomposition product solution was emitting a hydrogen sulfide-like odor.
この分解生成物溶液を皮膚に塗ってみると保湿効果が認
められたが、硫化水素臭が強く不快であった。更にこの
分解生成物溶液を試飲してみたところ、異臭が強く、ま
た味も悪く飲料素材として不適当であった。When this decomposition product solution was applied to the skin, a moisturizing effect was observed, but the hydrogen sulfide odor was strong and unpleasant. Furthermore, when I sampled this decomposition product solution, it had a strong off-odor and bad taste, making it unsuitable as a beverage material.
本発明の蛋白分解酵素処理の卵殻膜水溶性分解生成物は
淡黄色で異臭のほとんど感じられないものであり、化粧
品素材や食品素材としても優れているものである。The eggshell membrane water-soluble decomposition product obtained by the protease treatment of the present invention is pale yellow in color and has almost no off-odor, making it excellent as a cosmetic material or food material.
本発明は、卵殻膜の利用を拡大し、人の生活を豊にする
と共に化粧品および食品産業の発展に貢献するものであ
る。The present invention expands the use of eggshell membranes, enriches people's lives, and contributes to the development of the cosmetics and food industries.
Claims (1)
成する水溶性分解生成物を採取することを特徴とする水
溶性卵殻膜の製造法。A method for producing water-soluble eggshell membranes, which comprises treating avian eggshell membranes with one or more proteolytic enzymes and collecting the resulting water-soluble decomposition products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2271560A JPH07110210B2 (en) | 1990-10-08 | 1990-10-08 | Method for producing water-soluble eggshell membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2271560A JPH07110210B2 (en) | 1990-10-08 | 1990-10-08 | Method for producing water-soluble eggshell membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04148649A true JPH04148649A (en) | 1992-05-21 |
| JPH07110210B2 JPH07110210B2 (en) | 1995-11-29 |
Family
ID=17501782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2271560A Expired - Lifetime JPH07110210B2 (en) | 1990-10-08 | 1990-10-08 | Method for producing water-soluble eggshell membrane |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07110210B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001245634A (en) * | 2000-03-03 | 2001-09-11 | Q P Corp | Egg product |
| JP2007167031A (en) * | 2005-12-26 | 2007-07-05 | Riken Vitamin Co Ltd | Seasoning composition containing eggshell film |
| JP2008007419A (en) * | 2006-06-27 | 2008-01-17 | Ifuji Sangyo Co Ltd | Hydrolyzed shell membrane produced from shell membrane of hen's egg with proteinase, method for producing the same and functional material added therewith |
| US8580315B2 (en) * | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
| US10526423B2 (en) | 2010-08-31 | 2020-01-07 | Amano Enzyme Inc. | Eggshell membrane solubilization method using enzymes |
| CN114085880A (en) * | 2021-09-30 | 2022-02-25 | 厚德食品股份有限公司 | Method for extracting eggshell membrane hydrolysate and suction filtration device for extracting eggshell membrane hydrolysate |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7237291B1 (en) * | 2021-10-06 | 2023-03-13 | バイオサイエンステクノロジー株式会社 | Method for producing fermented decomposed eggshell membrane |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4840944A (en) * | 1971-10-07 | 1973-06-15 | ||
| JPS5423975B2 (en) * | 1971-10-07 | 1979-08-17 | ||
| JPS5545176B2 (en) * | 1971-10-07 | 1980-11-17 | ||
| JPS5611682B2 (en) * | 1971-10-07 | 1981-03-16 | ||
| JPH01275512A (en) * | 1988-04-27 | 1989-11-06 | Q P Corp | Production of soluble shell membrane |
-
1990
- 1990-10-08 JP JP2271560A patent/JPH07110210B2/en not_active Expired - Lifetime
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4840944A (en) * | 1971-10-07 | 1973-06-15 | ||
| JPS5423975B2 (en) * | 1971-10-07 | 1979-08-17 | ||
| JPS5545176B2 (en) * | 1971-10-07 | 1980-11-17 | ||
| JPS5611682B2 (en) * | 1971-10-07 | 1981-03-16 | ||
| JPH01275512A (en) * | 1988-04-27 | 1989-11-06 | Q P Corp | Production of soluble shell membrane |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001245634A (en) * | 2000-03-03 | 2001-09-11 | Q P Corp | Egg product |
| US8580315B2 (en) * | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
| JP2007167031A (en) * | 2005-12-26 | 2007-07-05 | Riken Vitamin Co Ltd | Seasoning composition containing eggshell film |
| JP4553836B2 (en) * | 2005-12-26 | 2010-09-29 | 理研ビタミン株式会社 | Seasoning composition containing eggshell membrane |
| JP2008007419A (en) * | 2006-06-27 | 2008-01-17 | Ifuji Sangyo Co Ltd | Hydrolyzed shell membrane produced from shell membrane of hen's egg with proteinase, method for producing the same and functional material added therewith |
| US10526423B2 (en) | 2010-08-31 | 2020-01-07 | Amano Enzyme Inc. | Eggshell membrane solubilization method using enzymes |
| CN114085880A (en) * | 2021-09-30 | 2022-02-25 | 厚德食品股份有限公司 | Method for extracting eggshell membrane hydrolysate and suction filtration device for extracting eggshell membrane hydrolysate |
| CN114085880B (en) * | 2021-09-30 | 2023-11-03 | 厚德食品股份有限公司 | Eggshell membrane hydrolysate extraction method and extraction and filtration device for extraction |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07110210B2 (en) | 1995-11-29 |
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