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JPH04126066A - Device for stirring cell, cell fusion apparatus and flow cytometer apparatus containing the same - Google Patents

Device for stirring cell, cell fusion apparatus and flow cytometer apparatus containing the same

Info

Publication number
JPH04126066A
JPH04126066A JP28477590A JP28477590A JPH04126066A JP H04126066 A JPH04126066 A JP H04126066A JP 28477590 A JP28477590 A JP 28477590A JP 28477590 A JP28477590 A JP 28477590A JP H04126066 A JPH04126066 A JP H04126066A
Authority
JP
Grant status
Application
Patent type
Prior art keywords
cell
suspension
valve
tube
apparatus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP28477590A
Other versions
JPH0710226B2 (en )
Inventor
Kazuhiro Fujita
Takashi Koyano
Kazuchika Sakamoto
Original Assignee
P C C Technol:Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/22Means for packing or storing viable microorganisms

Abstract

PURPOSE: To enable dispersion and maintenance of cells at a homogeneous and nearly constant cell density in a solution by repeating suction and discharge of a cell suspension from the tip of a nozzle.
CONSTITUTION: A gas pressurized with a pressurizing pump 15 is regulated to 0.05-1.0kg/cm2 pressure with a regulator 16, then fed through a filter 17, a manifold 18 and a valve 15 into a container 3 containing a cell suspension 21 and pressurized. A valve 6 is then opened to suck the suspension 21 from the tip of a nozzle 1 and temporarily held in a tube 2 to close the valve 6. Valves 10, 7 and 6 are subsequently opened to pressurize the tube 2 and the suspension 21 in the tube 2 is then discharged into the container 3. The aforementioned operation is repeated 1 to several times to stir the suspension 21, which is further passed through a flow passage 31 and discharged to the outside.
COPYRIGHT: (C)1992,JPO&Japio
JP28477590A 1990-06-20 1990-10-23 Protoplasts stirrer and cell fusion device and a flow cytometer apparatus comprising the same Expired - Lifetime JPH0710226B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2-161965 1990-06-20
JP16196590 1990-06-20

Publications (2)

Publication Number Publication Date
JPH04126066A true true JPH04126066A (en) 1992-04-27
JPH0710226B2 JPH0710226B2 (en) 1995-02-08

Family

ID=15745441

Family Applications (1)

Application Number Title Priority Date Filing Date
JP28477590A Expired - Lifetime JPH0710226B2 (en) 1990-06-20 1990-10-23 Protoplasts stirrer and cell fusion device and a flow cytometer apparatus comprising the same

Country Status (1)

Country Link
JP (1) JPH0710226B2 (en)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602349A (en) * 1994-10-14 1997-02-11 The University Of Washington Sample introduction system for a flow cytometer
US5602039A (en) * 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5643796A (en) * 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US6133044A (en) * 1994-10-14 2000-10-17 University Of Washington High speed flow cytometer droplet formation system and method
US6248590B1 (en) 1998-02-27 2001-06-19 Cytomation, Inc. Method and apparatus for flow cytometry
US6819411B1 (en) 1997-01-31 2004-11-16 Xy, Inc. Optical apparatus
US6861265B1 (en) 1994-10-14 2005-03-01 University Of Washington Flow cytometer droplet formation system
JP2007208108A (en) * 2006-02-03 2007-08-16 Tamura Seisakusho Co Ltd Material feeder and material feeding method
US7723116B2 (en) 2003-05-15 2010-05-25 Xy, Inc. Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
US7758811B2 (en) 2003-03-28 2010-07-20 Inguran, Llc System for analyzing particles using multiple flow cytometry units
US7772005B1 (en) 1998-07-30 2010-08-10 Xy, Llc Method of establishing an equine artificial insemination sample
US7838210B2 (en) 2004-03-29 2010-11-23 Inguran, LLC. Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations
US7855078B2 (en) 2002-08-15 2010-12-21 Xy, Llc High resolution flow cytometer
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
US9134220B2 (en) 2004-07-27 2015-09-15 Beckman Coulter, Inc. Enhancing flow cytometry discrimination with geometric transformation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427626A (en) * 1987-07-21 1989-01-30 Sanuki Kogyo Kk Method for agitating reagent

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427626A (en) * 1987-07-21 1989-01-30 Sanuki Kogyo Kk Method for agitating reagent

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6861265B1 (en) 1994-10-14 2005-03-01 University Of Washington Flow cytometer droplet formation system
US5602039A (en) * 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5643796A (en) * 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US5726364A (en) * 1994-10-14 1998-03-10 The University Of Washington Sample introduction apparatus for a flow cytometer
US6133044A (en) * 1994-10-14 2000-10-17 University Of Washington High speed flow cytometer droplet formation system and method
US5602349A (en) * 1994-10-14 1997-02-11 The University Of Washington Sample introduction system for a flow cytometer
US7923252B2 (en) 1994-10-14 2011-04-12 University Of Washington Droplet formation systems for flow cytometers
US6819411B1 (en) 1997-01-31 2004-11-16 Xy, Inc. Optical apparatus
US7929137B2 (en) 1997-01-31 2011-04-19 Xy, Llc Optical apparatus
US6248590B1 (en) 1998-02-27 2001-06-19 Cytomation, Inc. Method and apparatus for flow cytometry
US6589792B1 (en) 1998-02-27 2003-07-08 Dakocytomation Denmark A/S Method and apparatus for flow cytometry
US7772005B1 (en) 1998-07-30 2010-08-10 Xy, Llc Method of establishing an equine artificial insemination sample
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
US7855078B2 (en) 2002-08-15 2010-12-21 Xy, Llc High resolution flow cytometer
US7758811B2 (en) 2003-03-28 2010-07-20 Inguran, Llc System for analyzing particles using multiple flow cytometry units
US7799569B2 (en) 2003-03-28 2010-09-21 Inguran, Llc Process for evaluating staining conditions of cells for sorting
US7723116B2 (en) 2003-05-15 2010-05-25 Xy, Inc. Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
US7838210B2 (en) 2004-03-29 2010-11-23 Inguran, LLC. Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations
US9134220B2 (en) 2004-07-27 2015-09-15 Beckman Coulter, Inc. Enhancing flow cytometry discrimination with geometric transformation
USRE46559E1 (en) 2004-07-27 2017-09-26 Beckman Coulter, Inc. Enhancing flow cytometry discrimination with geometric transformation
JP2007208108A (en) * 2006-02-03 2007-08-16 Tamura Seisakusho Co Ltd Material feeder and material feeding method

Also Published As

Publication number Publication date Type
JP1989048C (en) grant
JPH0710226B2 (en) 1995-02-08 grant

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