JPH0377602A - Extraction unit - Google Patents
Extraction unitInfo
- Publication number
- JPH0377602A JPH0377602A JP21318789A JP21318789A JPH0377602A JP H0377602 A JPH0377602 A JP H0377602A JP 21318789 A JP21318789 A JP 21318789A JP 21318789 A JP21318789 A JP 21318789A JP H0377602 A JPH0377602 A JP H0377602A
- Authority
- JP
- Japan
- Prior art keywords
- support
- nozzle
- cleaning
- sample support
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 19
- 238000004140 cleaning Methods 0.000 claims abstract description 20
- 150000001413 amino acids Chemical class 0.000 claims description 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims 1
- 238000011109 contamination Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 125000002252 acyl group Chemical group 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Extraction Or Liquid Replacement (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、タンパク質あるいはペプチドのアごノｆＩＩ
組成分析の第１段階である加水分解を行う装置の試料支
持体からのアミノ酸の抽出ユニットに関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to protein or peptide agonofII.
This invention relates to a unit for extracting amino acids from a sample support in an apparatus that performs hydrolysis, which is the first step in compositional analysis.
（従来の技術〕
従来、試料支持体からのアミノ酸の抽出は、手操作によ
って少量の抽出溶媒を試料支持体に吸収させ、流出する
抽出溶液を集めることにより行われてきた。(Prior Art) Conventionally, amino acids have been extracted from a sample support by manually absorbing a small amount of extraction solvent into the sample support and collecting the flowing extraction solution.
しかし、上記方法は手操作であるため、熟練を必要とし
、タンパク質およびペプチドの加水分解操作のうち抽出
工程は個人誤差および汚染の原因工程の一つとされてい
た。However, since the above-mentioned method is a manual operation, it requires skill, and among the hydrolysis operations for proteins and peptides, the extraction step is considered to be one of the steps that cause individual errors and contamination.
そこで本発明は前記！！題を解決するために、タンパク
質およびペプチドの加水分解工程中のアミノ酸抽出工程
を自動化したものである。Therefore, the present invention is described above! ! In order to solve this problem, the amino acid extraction process during the protein and peptide hydrolysis process was automated.
まず、タンパク質およびペプチドを試料支持体中でア壽
ノ酸へ加水分解後、試料支持体の端面と密着する面を有
するノズルが装置された一対の対向して配置されたエア
ーシリンダと、前記ノズル中央の流路に接続したチュー
ブとを、前記試料支持体と前記ノズルと前記チューブと
で流体の流路を形成するように配置した抽出機構と、前
記ノズルの洗浄機構と、それらへの前記試料支持体およ
び洗浄用支持体の移送機構とを備えた構成としたもので
ある。First, after hydrolyzing proteins and peptides into acyl acids in a sample support, a pair of air cylinders arranged opposite to each other are equipped with nozzles having surfaces that come into close contact with the end surfaces of the sample support; an extraction mechanism in which a tube connected to a central flow path is arranged so that a fluid flow path is formed by the sample support, the nozzle, and the tube; a cleaning mechanism for the nozzle; The cleaning device is configured to include a support and a cleaning support transfer mechanism.
このような構威の抽出ユニットによれば、アミノ酸の抽
出工程を人手をかけずに、機械的に行うことができる。According to the extraction unit with such a structure, the amino acid extraction process can be carried out mechanically without any manual effort.
それによって、従来法の欠点であった操作の熟練性の排
除および抽出誤差の解消、さらに試料構成アノミ酸以外
のアミノ酸の混入除去を行うことができる。また、アミ
ノ酸の抽出工程と機械的に連続して行うこともできる。This makes it possible to eliminate operational skill and extraction errors, which are disadvantages of conventional methods, and to eliminate contamination of amino acids other than those constituting the sample. Moreover, it can also be carried out mechanically and continuously with the amino acid extraction step.
以下、本発明の実施例について図面に基づいて説明する
。Embodiments of the present invention will be described below based on the drawings.
第１図は、本発明による抽出ユニットの前面図である。FIG. 1 is a front view of a brewing unit according to the invention.
対向するエアーシリンダ】、２とノズル８．９およびチ
ューブ１０．１）が装着されるノズル支持プレート６．
７はレールの上に直線的に配置され、ピストン１２．１
３によりノズルプレート６゜７を移動させることによっ
て、試料支持体１４および洗浄用支持体１５をノズル８
，９で密着および脱着できる。前記ノズル８．９の端面
と前記試料支持体中４および前記洗浄用支持体１５の端
面が密着した時、抽出および洗浄流路が構威される。特
に、それらの端面ば精度よく加工されており、抽出およ
び洗浄液の漏れは防御できる。また、前記試料支持体１
４および洗浄用支持体１５が密着するノズル８．９の端
面ば全く異なっており、前記試料支持体１４とノズル８
，９とから流路を構威し、抽出液を流した後、前記ノズ
ル８，９の端面を前記洗浄用支持体１５と前記ノズル８
，９とから流路を構威し、洗浄液を流すことによって洗
浄できるように加工されている。2 and a nozzle support plate 6. on which the nozzle 8.9 and tube 10.1) are mounted.
7 is arranged linearly on the rail and the piston 12.1
3 by moving the nozzle plate 6°7, the sample support 14 and the cleaning support 15 are moved to the nozzle 8.
, 9 allows for close attachment and detachment. When the end face of the nozzle 8.9 and the end faces of the sample support medium 4 and the washing support 15 come into close contact, an extraction and washing flow path is established. In particular, their end faces are machined with high precision to prevent leakage of extraction and cleaning fluids. In addition, the sample support 1
4 and the nozzle 8.9 in close contact with the cleaning support 15 are completely different, and the sample support 14 and the nozzle 8.
, 9, and after flowing the extract, the end surfaces of the nozzles 8, 9 are connected to the cleaning support 15 and the nozzle 8.
, 9 form a flow path, and is processed so that cleaning can be performed by flowing a cleaning liquid.
次に、前記洗浄用支持体１５のノズル８．９への移送機
構について説明する。Next, a mechanism for transferring the cleaning support 15 to the nozzle 8.9 will be explained.
第２図（ａｌは、前記洗浄用支持体１５の移送機構の側
面図、第２図ω）はその正面図である。FIG. 2 (al is a side view of the transfer mechanism for the cleaning support 15, and FIG. 2 ω) is a front view thereof.
前記洗浄用支持体１５は、アーム１６に装着されたグリ
ップ１９によって保持され、前記アーム１６が９０゜回
転することによって前記ノズルプレート６．７の間に移
送される。その時の駆動はエアシリンダー１８によって
行われる。その際に、前記ノズル８゜９に密着していた
前記試料用支持体１４は、ノズル支持プレート６．７の
脱着的動きによってトレー１７へ回収保管される。The cleaning support 15 is held by a grip 19 attached to an arm 16 and is transferred between the nozzle plates 6.7 by rotating the arm 16 through 90 degrees. The drive at this time is performed by the air cylinder 18. At this time, the sample support 14, which was in close contact with the nozzle 8.9, is collected and stored in the tray 17 by the detachable movement of the nozzle support plate 6.7.
以上の操作は、パネルによって制御することができる。The above operations can be controlled by the panel.
以上の説明からも理解されるように、試料支持体からア
ミノ酸の抽出工程が自動化され、それによって、
１、熟練性の排除
２、抽出誤差の解消
３、試料の汚染防止
の３点で著しい効果がみられる。As can be understood from the above explanation, the process of extracting amino acids from the sample support is automated, resulting in significant effects in three ways: 1. Eliminating the need for skill, 2. Eliminating extraction errors, and 3. Preventing sample contamination. can be seen.
第１図は本発明による抽出ユニットの前面図′、第２図
（ａｌおよび（ｂｌはそれぞれ洗浄用支持体の移送機構
の側面図および正面図である。
１、２゜
３ ・ ・ ・
４、５ ・
６、７ ・
８、９ ・
１０．１）・
１２．１３・
１４・ ・ ・
１８・・・エアシリンダー
・・レール
・・ガイド
・・ノズル支持プレート
・・ノズル
・・チューブ
・・ピストン
・・試料支持体
１５・
１６・
・洗浄用支持体
・アーム
１７・
トレー
１９・
・グリップ
以
上Fig. 1 is a front view of the extraction unit according to the invention, Fig. 2 (al and (bl are respectively a side view and a front view of the transfer mechanism of the washing support. 1, 2゜3...4, 5 ・ 6, 7 ・ 8, 9 ・ 10.1)・ 12.13・ 14・ ・ ・ 18...Air cylinder...Rail...Guide...Nozzle support plate...Nozzle...Tube...Piston...・Sample support 15・ 16・ ・Cleaning support ・Arm 17・ Tray 19・ ・Grip or above
Claims (3)
る面を有するノズルが装着された対向して配置された一
対のエアシリンダーと、前記ノズル中央の流路に接続し
たチューブとを前記試料支持体と前記ノズルと前記チュ
ーブとで流体の流路を形成するように配置したことを特
徴とする抽出ユニット。(1) A pair of air cylinders arranged facing each other are equipped with nozzles having surfaces that come into close contact with the end surfaces of the sample support when the sample support is fixed, and a tube connected to the flow path in the center of the nozzles. An extraction unit characterized in that the sample support, the nozzle, and the tube are arranged so as to form a fluid flow path.
構成要素であるアミノ酸を担持することを特徴とする請
求項１記載の抽出ユニット。(2) The extraction unit according to claim 1, wherein the sample support supports amino acids that are constituents of proteins or peptides.
部とする空間を前記ノズルと前記チューブと洗浄用支持
体とで構成される洗浄機構を有する請求項１記載の抽出
ユニット。（４）前記洗浄用支持体をその中心軸が対向
する前記ノズルの中心軸と同一となるように移送する機
構を有することを特徴とする請求項１記載の抽出ユニッ
ト。(3) The extraction unit according to claim 1, further comprising a cleaning mechanism that includes the nozzle, the tube, and a cleaning support, and a space in which the nozzle and the sample support become part of the flow path. . (4) The extraction unit according to claim 1, further comprising a mechanism for transporting the cleaning support so that its central axis is the same as the central axis of the opposing nozzle.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21318789A JPH0377602A (en) | 1989-08-18 | 1989-08-18 | Extraction unit |
| EP19900302842 EP0388224A3 (en) | 1989-03-17 | 1990-03-16 | Method and apparatus for effecting chemical treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21318789A JPH0377602A (en) | 1989-08-18 | 1989-08-18 | Extraction unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0377602A true JPH0377602A (en) | 1991-04-03 |
Family
ID=16634981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21318789A Pending JPH0377602A (en) | 1989-03-17 | 1989-08-18 | Extraction unit |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0377602A (en) |
-
1989
- 1989-08-18 JP JP21318789A patent/JPH0377602A/en active Pending
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