JPH0331210A - Composition for oral cavity - Google Patents

Abstract

PURPOSE:To obtain a composition for oral cavity effective for preventing and remedying dental caries and periodontosis containing a polysaccharide having immunomodulating action. CONSTITUTION:A composition for oral cavity containing a polysaccharide (preferably lentinan, sizofiran or krestin) comprising at least one of glucose, galactose, mannose, arabinose, xylose, fructose, fucose, rhamnose, apiose, glucuronic acid and galacturonic acid as a constituent saccharide and having immunomodulating action. The polysaccharide as solution or cream is directly applied to the affected part or added to tooth paste, troche, chewing gum, etc., and used. The amount of the polysaccharide used is an amount to reach effective concentration of polysaccharide in the oral cavity (0.01-0.5wt.% in the case of lentinan and 0.05-2.5wt.% in the case of krestin).

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to an oral composition, and more particularly to an oral composition effective for preventing and treating caries and periodontal disease.

[Conventional technology and its problems]

Caries and periodontal disease are two diseases of the oral cavity, and their prevention,
Great efforts have been made since ancient times regarding treatment.

However, even in today's world of remarkable medical advances, these are among the diseases that humanity has not been able to completely overcome, and endless efforts have been made to overcome them. The law has been implemented. Particularly in recent years, new attempts have been made to investigate the prevention of caries via the immune system. For example, there are reports of attempts to prevent dental caries using antibodies that are antigenic to the cells or cell components of Streptococcus mutans, Batateroides gingivalis, or oral mixed bacteria that cause caries (Unexamined Japanese Patent Publication No. No. 60-38327, JP-A-60-
No. 38329, JP-A-60-142915, JP-A-6
1-112028, JP 61-112029, JP 61-112030, JP 61-277632
No., JP-A-61289024, JP-A-61-417). In addition, phenylalanine and the like are being investigated for the prevention of dental caries via the immune system (Japanese Patent Application Laid-Open No. 1471-1471).
No. 3).

On the other hand, prevention and treatment methods for periodontal disease via the immune system have been proposed, and coumarin (Japanese Patent Laid-open No. 6
No. 2-39516), glycyrrhetinic acid (Japanese Patent Application Laid-open No. 1983-
152622, JP-A No. 59-227812), and those using chitin and chitosan (JP-A-59-101416) have already been disclosed.

As mentioned above, there have been several attempts to prevent and treat caries and periodontal disease through immunity. However, still
The reality is that no truly effective method has been found.

[Means to solve the problem]

In view of the above circumstances, the present inventor conducted intensive research to obtain an oral composition effective in preventing and treating dental caries and periodontal disease. As a result, the present inventor found that polysaccharides with immunomodulatory effects are effective in preventing and treating these diseases. They found that it is effective for treatment and completed the present invention.

That is, the present invention provides an oral composition characterized by containing a polysaccharide having an immunomodulatory effect.

The polysaccharide that is the active ingredient of the present invention has at least one constituent sugar among glucose, galactose, mannose, arabinose, xylose, fructose, fucose, rhamnose, abiose, glucuronic acid, and galacturonic acid, and has an immunomodulatory effect. It is. Specifically, for example, lentinan, U-pahiman, hydroxyethylpahiman, schizophyllan, scleroglucan, linear lentinan, bahimaran, busturan, krestin,
Tokiarabinogalakukun, Souyou acidic heteropolysaccharide,
Examples include yokuinin acidic heteropolysaccharide and shikonin neutral polysaccharide. Among these, lentinan, schizophyllan, krestin, etc. are particularly preferred. Although these polysaccharides can be used alone, a plurality of polysaccharides may be used as a mixture. The effective concentration of these polysaccharides differs depending on the type of polysaccharide and cannot be generalized, but for example, for lentinan or schizophyllan, the effective concentration is 0.001 to 1% by weight (hereinafter simply referred to as "%"), preferably 0.001% to 1% by weight (hereinafter simply referred to as "%"). O1-0.5%, Krestin 0.05-5%, preferably 0.05-2.5%. Therefore, the oral composition of the present invention may contain these polysaccharides in an amount that will reach the concentration in the oral cavity.

In addition, as other components of the oral composition of the present invention, components used in conventional oral compositions can be used as appropriate depending on the purpose and mode of use. For example, in the case of toothpaste, abrasives such as calcium diphosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, amorphous silica, aluminosilicate, aluminum oxide, aluminum hydroxide, and resin; carboxymethyl cellulose, hydroxyethyl cellulose ,
Binder such as alginate, caragetin, gum arabic, polyvinyl alcohol; Foaming agent such as sodium nilauryl sulfate, sodium dodecylbenzenesulfonate, sodium N-lauroyl sarcosinate, N-acylglutamate, sucrose fatty acid ester; peppermint, spearmint essential oils such as; fragrances such as l-menthol, carvone, eugenol, anethole; sweeteners such as saccharin sodium, stevioside, neohesberidyl dihydrochalcone, glycyrrhizin, verlaritin, p-methoxycinnamic aldehyde; ingredients such as preservatives with water. mix,
Manufactured according to conventional methods. In addition, in mouthwashes and other oral cleansing agents, ingredients are appropriately blended according to the properties of the product.

In addition, the oral composition of the present invention contains sodium chloride, vitamin C1 vitamin E2 nicotinic acid ester, allantoin chlorhydroxyaluminum, azulene, and water-soluble primary or dilinic acid as active ingredients other than the polysaccharide having an immunomodulating effect. acid salts, quaternary ammonium compounds,
Fluorinated compounds, lysozyme chloride, hinokitiol, zeolite, protease, and crude drug extracts can also be further blended.

The polysaccharide used in the present invention can be applied directly to the affected area as a liquid or cream, or can be applied to toothpaste, mouthwash, etc.
It can be included in troches, chewing gum, etc., and can also be used as a preparation that sticks to the oral mucosa.

〔Effect of the invention〕

The oral composition of the present invention is extremely effective in preventing and treating caries and periodontal disease compared to existing oral compositions.

〔Example〕

Next, the present invention will be explained in more detail with reference to Examples and Test Examples, but the present invention is not limited thereto.

Example 1 Effect on periodontitis animal model: The effect of the polysaccharide used in the oral composition of the present invention on periodontitis was investigated using a periodontitis model animal.

As a model animal, naturally occurring periodontitis weighs approximately 14 kg.
g. Using three 4-year-old female pegle dogs, the molars were divided into four parts, top, bottom, left, and right, and one part was treated with a 2.0% carboxymethylcellulose aqueous solution containing 0.1% lentinan, and the other part was treated with a 2.0% carboxymethylcellulose aqueous solution containing 0.1% lentinan.
A 2.0% carboxymethylcellulose aqueous solution containing 0.1% schizophyllan was applied to one of the two, and 2.0% carboxymethylcellulose alone was applied to the other as a control for 10 minutes. The application was performed after anesthesia and after removing saliva from the molar gingiva.

Such drug treatment was performed once a day for 8 days, and the gingival index, the amount of intergingival sulcus exudate, and the number of bacteria were measured on the 0th, 3rd, and 8th days. The average of the data from the three animals was determined and used as the measured value.

Gingival index (Shi Oe et al.; J, ofP
eriodontolo, 38.610-616.1
967) was measured by visual observation by two people using the following evaluation to see the difference in detail.

Score 0.5 1.0 1.5 2.0 3.0 Progression level of gingivitis Clinically normal gingiva.

Her face is normal, but there is some redness on her gums.

Mild inflammation of the gums. There was no bleeding when I touched the pocket with the probe.

When I touch my pocket with a probe, it doesn't bleed, but it's still normal.

Moderate inflammation of the gums. When I touch my pocket with a probe, it bleeds.

Severe inflammation of the gums. spontaneous bleeding.

To measure the amount of intergingival sulcus exudate, first wipe the sampling site with a tissue paper and wipe it with period paper (Interstate Drug Excha) to prevent saliva from getting mixed in.
nge (trade name) was inserted into the gingival sulcus for 10 seconds, the first sample was discarded, and the second and third measurements were taken at the same site, and the average was taken as the measured value.

The measurement was performed using Periodron (manufactured by IARCO, trade name), and the readings of the meter were used as the measured values.

The number of bacteria was determined by collecting intergingival sulcus exudate using a paper point (manufactured by Johnson & Johnson), inoculating it into BHI medium containing 5% sheep blood, culturing it anaerobically for 5 days, and counting the number of colonies formed. It was measured.

Figure 1 shows the change in the gingival index over time, Figure 2 shows the change in the amount of intergingival sulcus exudate over time, and Figure 3 shows the change in the number of bacteria over time.

The results showed that the gingival index, amount of intergingival sulcus exudate, and bacterial count were clearly reduced in those treated with lentinan or schiffilan.

Example 2 Paste dentifrice: Lentinan 0.10 (%) Calcium phosphate for toothpaste 30.00 Silicic anhydride 2.00 Carboxymethyl cellulose Sodium lauryl sulfate Glycerin 70% Sorbitol liquid Flavor Purified water Total Example 3 Creamy gum liniment Niji Sifiran Monoethanolamine Liquid Paraffin Polyvinyl Alcohol Fatty Acid Monoglyceride Glycerin Flavor Powder Sucrose Total Example 4 Trotinylentinane Citric Acid 2.00 0.10 10,00 15.00 0.80 Appropriate Amount 100.00 0.50 (%> 0 .10 7.00 5.00 3.00 15,00 0, 1O Appropriate amount 100,00 0.50 (%) 0.10 Aspartame dextrin Gum arabic flavoring Lactose 7.00 5.00 3.00 Microfluorescence Appropriate amount total 100 .D.

Example 5 Mouthwash 5 Nijishifiran 0.50 (%)
Ethanol 10. DO monoethanolamine 0.10 Chlorhexidine gluconate 0.10 Pluronic type 1'
108 0.50 Fragrance 0
．． 20 Purified water appropriate amount total
100. D.

[Brief explanation of drawings]

FIG. 1 shows the change over time in the gingival index, FIG. 2 shows the change over time in the amount of intergingival sulcus exudate, and FIG. 3 shows the change over time in the number of bacteria. In addition, in FIGS. 1 to 3, Δ-Δ indicates a lentinan aqueous solution, Rolo indicates a Schifffilan aqueous solution, and ○-○ indicates a control.

Claims (1)

[Claims] 1. An oral composition characterized by containing a polysaccharide having an immunomodulatory effect.