JPH03258723A - Adhesion-protecting material - Google Patents

Abstract

PURPOSE:To provide the subject adhesion-protecting material produced from chitin, capable of prevention of adhesion in a living body, especially postoperative adhesion of a wound due to an surgical operation, receiving a low excretory effect of foreign matter from the living body and absorbable in the living body without remaining therein as a foreign matter. CONSTITUTION:Chitin is used as an adhesion-protecting material and the adhesion-protecting material can effectively protect adhesion of a wound or a section in a living body or a postoperative adhesion of a wound due to a surgical operation. As the above-mentioned adhesion-protecting material is free from occurrence of an abnormal reaction in the living body, absorbable in the living body and disappears after a certain period, it is useful for normal recovery of the diseased part. As chitin, a deacetylated chitin obtained by treating exoskeleton of a Crustacea, an Insecta, etc., with hydrochloric acid and caustic soda for removal of ash and proteins and subsequently treating the resultant poly(N-acetyl-D-glucosamine) or poly(N-acetyl-D-glucosamine) with an alkali is used.

Description

Detailed Description of the Invention (Industrial Application Field) The present invention relates to an anti-adhesion material made of chitin, and particularly in the medical field, adhesion prevention material between living tissues that occurs during the process of biological repair after surgery, etc. The present invention relates to an anti-adhesion material suitable for preventing adhesion.

(Prior Art) Generally, it is well known that when a defect occurs in living tissue, the defective tissue adheres to other defective tissue or normal tissue during the repair process of the living body. Especially 1
Adhesions are highly likely to occur after internal surgical procedures. This adhesion.

This often interferes with the normal movement of organs.

In the worst case, it may become life-threatening.

As a countermeasure, for example, 9 cases have been used during surgical operations.

When suturing the incisions, try not to expose the sutured surface, or apply vegetable or animal oil to the affected area.

Previously, methods were used to prevent adhesions by applying cloth or film to the affected area.

(Problems to be Solved by the Invention) However, there are limits to such measures. That is, 2
No matter how much the surgical technique was used during surgery, adhesions still often occurred, and even when vegetable oil or animal oil was applied to the affected area, these oils tend to be removed by biological fluids and may not be fully effective. There were many. When applying cloth or film to the affected area, it is desirable to use a material that is biocompatible and does not react with foreign substances, but such materials are limited and are still bioabsorbable and biocompatible. None had been put into practical use.

The present invention effectively prevents adhesions on wounds, incisions, or after surgical operations, does not cause abnormal reactions in the living body, and
The object of the present invention is to provide an anti-adhesion material that is bioabsorbable and disappears over time.

(Means for Solving the Problems) As a result of intensive research to achieve the above-mentioned objects, the present inventors discovered that chitin can achieve the above-mentioned objects, and thus arrived at the present invention.

That is, one aspect of the present invention is an anti-adhesion material made of chitin.

Hereinafter, nine aspects of the present invention will be described in detail.

Chitin is poly(N-acetyl-D-glucosamine) or this poly(N-acetyl-D-glucosamine) obtained by treating the exoskeleton of crustaceans, insects, etc. with hydrochloric acid and caustic soda to remove ash and protein. -glucosamine) obtained by treating with an alkali (there are products with a degree of deacetylation ranging from a few percent to 100%). The material that does this is called chitosan.

The chitin used in the present invention is such chitin or chitosan, and furthermore, the glucosamine residue 10H group of chitin or chitosan.

-CH, OH group, -NH2 group, or -NHCOCH
Derivatives in which three groups are substituted with other groups (9) such as esterification, etherification, carboxymethylation (1), hydroxyethylation or 0-ethylation are also included.

Deacetylation of chitin can be carried out by the well-known method of treating chitin with an alkali.

At this time, the degree of deacetylation can be easily adjusted by appropriately changing the alkali concentration, treatment temperature, treatment time, etc. used.

The degree of deacetylation of chitin used in the present invention is not particularly limited.

The anti-adhesion material of the present invention is made of the chitin described above, and its forms include powder, aqueous solution, cotton-like material, film, non-woven fabric, porous material, etc.

The powdered anti-adhesion material of the present invention can be obtained by using purified chitin and pulverizing it with a pulverizer, and the particle size of the powdered chitin is preferably fine.
A mesh or higher is preferable.

The solution-form anti-adhesion material of the present invention can be prepared by dissolving water-soluble chitin (for example, chitosan or carboxymethyl chitin) in water or an acetic acid aqueous solution, and the concentration of the water-soluble chitin is 0.5 to 5. It is preferably about % by weight.

Cotton material, film, and nonwoven fabric of the present invention.

The anti-adhesion material such as a porous body can be formed into a bottom shape by a well-known wet method. That is, in order to obtain a molded article, chitin may be dissolved in a solvent to form a solution, and the solution may be coagulated using a coagulating liquid that is a non-solvent. A suitable solvent can be selected depending on the chemical structure of chitin. For example, in the case of p-(N-acetyl-D-glucosamine) with a low degree of deacetylation, a mixture of ) lychloroacetic acid and a chlorinated hydrocarbon;
Dimethylacetamide or a mixture of N-methylpyrrolidone and lithium chloride is used, and in the case of chitosan with a high degree of deacetylation, an aqueous acid solution such as acetic acid is used.

Moreover, water, alcohol, ketones, etc. are used as the coagulating liquid. Films and porous bodies can be formed by wet molding in some cases, but cotton-like bodies and single-knit nonwoven fabrics can be produced by a conventional method after first producing chitin fibers. The manufacturing method for these molded products is “Application of chitin and chitosan” (Gihodo Publishing, p. 99, 1990)
It is described in etc.

These anti-adhesion materials of the present invention have various shapes.

It can be used as an anti-adhesion material usually associated with surgical operations, and is mainly used in vivo, such as in the esophagus and stomach.

Small intestine, large intestine, gallbladder, pancreas, liver, kidneys, heart.

It may be applied to the affected area during incision, suturing, repair surgery, etc. of internal organs such as the peritoneum, uterus, and bladder. In the case of a powder or aqueous solution, it can be applied to the affected area, and in the case of a film, nonwoven fabric, fiber cloth, etc., it can be applied to the affected area as a patch material and used to isolate it from other organs. To prevent the patch material from moving during the period when the anti-adhesion effect is needed, it is particularly effective to sew it to the affected area with sutures or the like.

The anti-adhesion material of the present invention has the effect of preventing the affected area from adhering to other organs after use, and also biodegrades and disappears after the treatment of the affected area is completed. This decomposition is carried out by enzymes in the body.

The main enzymes include lysozyme and β-N-acetyl-glucosamidase. The time required for biodegradation varies depending on the shape of the anti-adhesion material, but 1
Usually it is 3 to 5 months.

(Examples) The present invention will be explained in more detail below using examples.

Example 1 White chitin powder (degree of deacetylation 2.5%, 0.1 w in a solution of dimethylacetamide:lithium chloride = 9/1)
/w% solution viscosity 220 centipoise, manufactured by Shin Nippon Chemical Co., Ltd.) was added to a mixed solution of dimethylacetamide and lithium chloride (weight ratio 9:1) at room temperature for 9 w.
/w% to create a clear, viscous solution. After filtering this solution with 2000 mesh stainless steel net,
Wet spinning was performed by degassing under reduced pressure, transporting with a gear pump, and discharging from a nozzle into water at 80°C.

The discharged yarn was wound on a bobbin at a speed of 10 m/min to obtain long fibers of 150 denier and 50 filaments. The obtained long fibers had a strength of 3.5 g/d.

The elongation was 12.4%.

The obtained long fibers are knitted using a circular knitting machine (manufactured by Kobo Kikai Seisakusho).

CR-B type) and circular knit the cloth 10 times x 10c.
It was cut into pieces of m size and sterilized with ethylene oxide gas. Meanwhile, after the rabbit was anesthetized, the abdomen was opened.

The stomach was removed and two 2 cm wide incisions were made, each of which was sutured with polyester sutures, one of the incisions was covered with the chitin cloth of the present invention, and the four sides were easily fixed with polyester sutures. The other wounds were left in place and the abdomen was closed again with polyester sutures.

Ten days later, the abdomen was opened again and the incision in the stomach was observed.
There was no adhesion of other organs to the wound where chitin cloth was applied, whereas the small intestine and liver were adhered to the wound where chitin cloth was not applied through strong connective tissue.

Example 2 Chitosan powder (deacetylation degree 85%, dissolution viscosity after dissolving in 0.2% acetic acid aqueous solution is 580 centiboise, Shin Nihon Kagakubu) was dissolved at 1 w/w% in 0.4% acetic acid aqueous solution. A chitosan solution was made. on the other hand.

The rabbit was anesthetized and a 5 cm long incision was made in the abdomen.

In both cases, the peritoneum was completely incised. The above-mentioned chitosan solution was sufficiently applied to the peritoneum of one of the wounds while removing body fluid. After application, the wound was sutured with polyester sutures, and chitosan solution was applied again from above. The other wounds were sutured with polyester sutures.

One week later, when the rabbit was sacrificed and examined, no adhesion was observed from the inside of the stomach, small intestine, etc. in the wound where the chitosan solution was applied, whereas strong adhesions and adhesions were observed in the wound where the chitosan solution was not applied. Connective tissue adhesions were observed.

Example 3 Chitosan powder (deacetylation degree 88%, dissolution viscosity after dissolving in 0.2% acetic acid aqueous solution is 894 centiboise, Shin Nippon Kagakubu) was dissolved in 1% acetic acid aqueous solution at 2 w/w% to make a chitosan solution. Had made. Place this on a glass plate.
When the film was cast to a thickness of 52 μm and heat treated in a hot air dryer at 70° C. for 2 hours, a chitosan film with a thickness of 52 μm was obtained. Meanwhile, an adult mongrel dog was anesthetized, the abdomen was opened, and the small intestine was removed. Two sections of the small intestine were cut into rings, and the entire circumference of each section was sutured with polypropylene sutures for repair. After suturing, one side was wrapped with chitosan film all around.

It was lightly secured to the small intestine wall with sutures. The other side was left alone and closed.

The dogs were kept on the same food as before the surgery, and 15 days later, they were sacrificed in 9 layers and their small intestines were observed. No adhesion of other organs was observed in the area where the chitosan film was wrapped, whereas in the area where the chitosan film was wrapped, the dog's small intestine was observed. Strong adhesions of the stomach, liver, pancreas, and connective tissue were observed in the areas where no adhesions were attached.

(Effects of the Invention) The anti-adhesion material of the present invention can prevent wounds generated in vivo, especially during surgical operations, from adhering after surgery, has a small foreign body reaction with living organisms, and is bioabsorbable. It disappears after its role is completed and does not remain as a foreign substance in the body. Therefore, it is of course not necessary even after reoperation, and is useful for normal repair of the affected area.

Claims (1)

[Claims] (1) Anti-adhesion material made of chitin.