JPH03232441A - 受胎率を上げた凍結受精卵およびその調製法 - Google Patents

Info

Publication number

JPH03232441A

JPH03232441A JP3033290A JP3033290A JPH03232441A JP H03232441 A JPH03232441 A JP H03232441A JP 3033290 A JP3033290 A JP 3033290A JP 3033290 A JP3033290 A JP 3033290A JP H03232441 A JPH03232441 A JP H03232441A

Authority

JP

Japan

Prior art keywords

fertilized egg

frozen

fertilized

straw

cryoprotectant

Prior art date

1990-02-09

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 239000010902 straw Substances 0.000 claims abstract description 41
• 239000000126 substance Substances 0.000 claims abstract description 11
• 229920000742 Cotton Polymers 0.000 claims abstract description 10
• 235000013601 eggs Nutrition 0.000 claims description 88
• 238000000034 method Methods 0.000 claims description 42
• 239000002577 cryoprotective agent Substances 0.000 claims description 30
• 229930006000 Sucrose Natural products 0.000 claims description 11
• 239000005720 sucrose Substances 0.000 claims description 11
• 238000000338 in vitro Methods 0.000 claims description 8
• 238000012546 transfer Methods 0.000 claims description 7
• 244000144972 livestock Species 0.000 claims description 4
• 239000000203 mixture Substances 0.000 claims description 2
• 241001494479 Pecora Species 0.000 claims 1
• 238000003163 cell fusion method Methods 0.000 claims 1
• 238000007789 sealing Methods 0.000 claims 1
• 125000000185 sucrose group Chemical group 0.000 claims 1
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 32
• 239000000243 solution Substances 0.000 abstract description 31
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 18
• 235000011187 glycerol Nutrition 0.000 abstract description 16
• 239000007788 liquid Substances 0.000 abstract description 13
• 229910052757 nitrogen Inorganic materials 0.000 abstract description 9
• 238000002844 melting Methods 0.000 abstract description 8
• 230000008018 melting Effects 0.000 abstract description 8
• 238000010438 heat treatment Methods 0.000 abstract description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
• 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
• 239000000872 buffer Substances 0.000 abstract 1
• 239000003795 chemical substances by application Substances 0.000 abstract 1
• 238000010257 thawing Methods 0.000 description 23
• 238000007710 freezing Methods 0.000 description 21
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• 238000002054 transplantation Methods 0.000 description 18
• 241000283690 Bos taurus Species 0.000 description 11
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 10
• 230000035899 viability Effects 0.000 description 7
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• CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
• 241000283973 Oryctolagus cuniculus Species 0.000 description 2
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• 150000004043 trisaccharides Chemical class 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
• YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 1
• 101100440696 Caenorhabditis elegans cor-1 gene Proteins 0.000 description 1
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
• HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
• HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
• 238000009395 breeding Methods 0.000 description 1
• 230000001488 breeding effect Effects 0.000 description 1
• 239000007853 buffer solution Substances 0.000 description 1
• 229910002092 carbon dioxide Inorganic materials 0.000 description 1
• 239000001569 carbon dioxide Substances 0.000 description 1
• 210000004027 cell Anatomy 0.000 description 1
• 238000004140 cleaning Methods 0.000 description 1
• 230000001010 compromised effect Effects 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• 238000005138 cryopreservation Methods 0.000 description 1
• 208000017338 epidermoid cysts Diseases 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 239000003337 fertilizer Substances 0.000 description 1
• 230000004927 fusion Effects 0.000 description 1
• WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
• 238000007654 immersion Methods 0.000 description 1
• 238000002513 implantation Methods 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000003780 insertion Methods 0.000 description 1
• 230000037431 insertion Effects 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 230000035800 maturation Effects 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 230000027758 ovulation cycle Effects 0.000 description 1
• 230000035699 permeability Effects 0.000 description 1
• 230000008569 process Effects 0.000 description 1
• ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 238000012797 qualification Methods 0.000 description 1
• 210000000582 semen Anatomy 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 238000005406 washing Methods 0.000 description 1

Cited By (3)