JPH0193508A - Cucumber extract containing superoxide dismutase in high concentration and production thereof - Google Patents

Abstract

PURPOSE:To readily obtain the titled substance useful as a raw material for cosmetics at a low cost, by heat-treating an extract of a cucumber fruit, removing the formed precipitates and adding sodium citrate. CONSTITUTION:A cucumber fruit is sufficiently crushed and pressed to remove tissual residues and provide a green suspendible extract, which is then heat- treated at 40-55 deg.C for 10-100min (preferably at 45-50 deg.C for 10-30min) to accelerate formation of insoluble pectin and aggregation of chloroplast and afford a clear light yellow extract by a method for filtration, etc. Sodium citrate is then added to the extract so as to provide 0.2-2.0% final concentration to provide a stable cucumber extract having a high concentration of superoxide dismutase (>=200units/ml) without any change with time. The superoxide dismutase is capable of inhibiting toxicity of active oxygen in living bodies and contriving improvement in skin tissues.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to superoxide dismutase (Super
Oxide Dismutase E C1,15,1
, 1, hereinafter referred to as SOD) in a high concentration, and a method for producing the same.

[Conventional technology]

Traditionally, cucumber fruit has been widely enjoyed as a food;
The extract is added to cosmetics as cucumber extract and is effective against burns, eczema, etc., so it is included in the General Purpose Cosmetic Ingredients (JCID) created by the Japan Cosmetic Industry Federation, and demand for it will continue to grow in the future. There is a tendency to

[Problem that the invention seeks to solve]

However, the active ingredients in cucumber extract have not been clarified, and the type of cucumber fruit,
Since the types and amounts of ingredients contained can vary significantly depending on the time of collection, extraction method, storage conditions, etc., it has traditionally been considered appropriate to evaluate the quality of cucumber extract using various biological activities. It was getting worse. However, measuring biological activity often requires complicated operations and is currently difficult to carry out in practice.

From the above-mentioned background, there has been a strong desire to discover the active ingredients of cucumber extract and to develop a highly effective cucumber extract.

On the other hand, SOD is an enzyme that catalyzes the disproportionation reaction of superoxide radicals generated by one-electron reduction of oxygen molecules.
By scavenging radicals, it has the ability to prevent damage caused by superoxide radicals themselves and other active oxygen species that result from them, so in recent years it has been used to treat rheumatoid arthritis, osteoarthritis, urinary tract infections, and side effects during radiation therapy. Attempts are being made to use it. SOD has been purified in large quantities from bovine red blood cells and its properties have been clarified, and its presence has been confirmed in plants such as spinach, mung bean, wheat germ, and rhinoceros. No reports have been made.

[Means to solve the problem]

In view of the current situation, the present inventors have conducted extensive research on the active ingredients in cucumber extract, using the effectiveness on the skin, such as stains, freckles, and rough skin, as indicators, and have confirmed the existence of multiple active ingredients. We discovered that SOD is one of the important components. It is thought that SOD cooperates with other active ingredients to improve skin tissue by scavenging superoxide radicals, which are the main cause of peroxide production, and strongly suppressing the toxicity of active oxygen. Furthermore, it has been revealed that SOD is extremely easy to measure its content and is useful as an indicator for quality evaluation of cucumber extract. Furthermore, since SOD is unstable to heat, chemical treatments, etc., this evaluation method is valuable as proof that it is extracted from cucumber fruit in its natural state. However, when we measured the SOD content in cucumber extracts currently on the market, we found that there were no cucumber extracts that contained SOD of 10 units/m or more. No consideration was given to this at all, and it was thought that the activity had been deactivated for some reason during the manufacturing process. As a result of further research, the present inventors succeeded in inventing a method for producing a cucumber extract that contains SOD at a high concentration and has high effectiveness.

That is, the present invention provides a cucumber extract containing a high concentration of SOD and a method for producing the same.The present invention will be explained in detail below.

As raw materials in the present invention, fresh Cucurbitaceae cucumbers (Cucurbitaceae) are used as raw materials.
-mis 5ativus) and its frozen products are used, but any variety can be used, and there are no particular limitations on the timing of collection.

In the present invention, the green suspension extract obtained by first sufficiently crushing the raw material and then squeezing to remove tissue residue is processed at 40 to 55°C for 10 to 10 minutes, preferably at 45 to 50°C. Heat treatment is performed for 10 to 30 minutes. This operation promotes the production of insoluble pectin and the aggregation of chloroplasts, and a clear pale yellow extract can be easily obtained by methods such as filtration. Moreover, at the same time, this operation can remove the polymeric substance as a precipitate, thereby reducing the antigenicity of the resulting product and improving its stability. During this process, no deactivation of other active ingredients including SOD was observed. Furthermore, by adding sodium citrate to this extract to a final concentration of 0.2 to 2.0%, it is possible to produce a cucumber extract that does not change over time, is stable, and contains SOD at a high concentration. The cucumber extract thus obtained is further added with preservatives such as parabens, filtered for sterilization, and then put into practical use.

S of cucumber extract produced by the method of the present invention
The OD concentration is more than 200 units/m1, which is a very high concentration compared to that of cucumber extract produced by conventionally known methods, and other active ingredients are also present in high concentrations without being denatured. Therefore, its usefulness is clear.

[Effect]

The effectiveness of the cucumber extract containing a high concentration of SOD of the present invention obtained in this manner is compared with known cucumber extracts in the following test examples.

In addition, the activity of Sop was determined according to the method of Matsucord and Fleetpitch (The Journal of Biological Chemistry, Vol. 244, p. 6049, 1969), and the amount that inhibits the reduction rate of cytochrome C by 50% is defined as one unit. did.

Test Example 1 Panel Test Each lotion containing the cucumber extract of the present invention and the known cucumber extract as shown below was used for 3 months on 100 women aged 18 to 44 (average age 24.5). Table 1 shows the sensory evaluation of the results. The evaluation items are A: gloss, B: moisture, C: transparency, D: rough skin, E: stains/freckles, F: pimples, and G: wrinkles.

Lotion formulation Cucumber extract 5.0g Glycerin 5.0g Ethanol 5.0g Methylparaben 0.1g Purified water Amount As is clear from Table 1, the cucumber extract of the present invention is highly effective compared to known cucumber extracts. It has become clear that it shows gender. .

〔Example〕

Examples of the present invention are shown below, but the present invention is not limited thereto.

Example 1 50 kg of fresh cucumber fruit was thoroughly crushed using a mixer, and tissue residue was removed using an automatic rotary compressor to obtain 43 kg of a green suspended extract. After heat-treating this at 50°C for 20 minutes and filtering out the resulting insoluble matter, 0.4 kg of sodium citrate was added to 41 kg of the resulting clear extract, followed by 0.1 kg of methylparaben and 2 kg of ethanol.
was added and filtered for sterilization to obtain 43 kg of cucumber extract.

The ice crystals were a clear liquid with a pale yellow color and a slight odor, and the SOD activity was 310 units/m1.Example 2 The suspended extract obtained in Example 1 was heat-treated at 45°C for 40 minutes. After removing the resulting insoluble matter by centrifugation,
40 kg of the resulting clear extract contains 0 sodium citrate.
．． Then, 0.05 kg of methylparaben and 0.005 kg of propylparaben were added and sterilized and filtered to obtain 40 kg of cucumber extract.

The ice crystals were a clear liquid with a pale yellow color and a slight odor, and the SOD activity was 260 units/ml. Example 3 <Lotion> Polyoxyethylene polyoxybrobylene alkyl ether 1. Og Cucumber extract of the present invention 2.0g Sorbitol 3.0g Sodium pyrrolidone carboxylate 3.0g Ethanol
10.0g methylparaben
0.1g purified water
Appropriate amount total amount 100. Og Example 4 <Emollient cream> Polyethylene glycol fatty acid ester 2.0g Glycerin fatty acid ester 5.0g MC stearic acid 5.0g Behenyl alcohol 1.0g Liquid paraffin
10.Og polyhydric alcohol fatty acid ester 10. OgCucumber extract of the present invention
1.0g1.3-butylene gellicol 5
．． 0g Methylparaben 0.1g
Butylparaben 0.1g Purified water Appropriate amount Total amount 100.0g [Effects of the invention] As detailed above, the cucumber extract containing a high concentration of SOD of the present invention is highly effective and useful as a cosmetic raw material. According to the present invention, it is possible to inexpensively and easily supply a stable cucumber extract containing SOD at a much higher concentration than conventionally known methods using cucumber fruit as a raw material, which is of great industrial significance.

Claims (1)

[Scope of Claims] 1) A cucumber extract characterized by containing a high concentration of superoxide dismutase. 2) A cucumber extract according to claim 1, which has a superoxide dismutase concentration of 200 units/ml or more. Extract 3) Extract obtained from cucumber fruit at 40-55℃
A method for producing a cucumber extract containing a high concentration of superoxide dismutase, which is characterized by adding sodium citrate to a final concentration of 0.2 to 2.0% after removing the resulting precipitate by heat treatment for 5 to 100 minutes.