JP7808298B2 - ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物 - Google Patents

ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物

Info

Publication number
JP7808298B2
JP7808298B2 JP2022547004A JP2022547004A JP7808298B2 JP 7808298 B2 JP7808298 B2 JP 7808298B2 JP 2022547004 A JP2022547004 A JP 2022547004A JP 2022547004 A JP2022547004 A JP 2022547004A JP 7808298 B2 JP7808298 B2 JP 7808298B2
Authority
JP
Japan
Prior art keywords
gene
h1foo
cells
protein
ips
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022547004A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2022050419A1 (https=
Inventor
晃 國富
恵一 福田
慎介 湯浅
亜峰 朱
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heartseed Inc
Original Assignee
Heartseed Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heartseed Inc filed Critical Heartseed Inc
Publication of JPWO2022050419A1 publication Critical patent/JPWO2022050419A1/ja
Application granted granted Critical
Publication of JP7808298B2 publication Critical patent/JP7808298B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C12N2506/115—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells from monocytes, from macrophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18841—Use of virus, viral particle or viral elements as a vector
    • C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022547004A 2020-09-04 2021-09-06 ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物 Active JP7808298B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020149447 2020-09-04
JP2020149447 2020-09-04
PCT/JP2021/032734 WO2022050419A1 (ja) 2020-09-04 2021-09-06 ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物

Publications (2)

Publication Number Publication Date
JPWO2022050419A1 JPWO2022050419A1 (https=) 2022-03-10
JP7808298B2 true JP7808298B2 (ja) 2026-01-29

Family

ID=80491146

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022547004A Active JP7808298B2 (ja) 2020-09-04 2021-09-06 ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物

Country Status (5)

Country Link
US (1) US20230220025A1 (https=)
EP (1) EP4209593A4 (https=)
JP (1) JP7808298B2 (https=)
CN (1) CN116018402A (https=)
WO (1) WO2022050419A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015519898A (ja) 2012-05-21 2015-07-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 合成自己複製ＲＮＡによるヒトｉＰＳ細胞の作製
WO2017010080A1 (ja) 2015-07-10 2017-01-19 Ｈｅａｒｔｓｅｅｄ株式会社 高品質なｉＰＳ細胞の製造方法
JP2020511529A (ja) 2017-03-03 2020-04-16 オブシディアン セラピューティクス， インコーポレイテッド 免疫療法のためのｃｄ１９組成物及び方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0864645T3 (da) 1995-10-31 2005-10-24 Dnavec Research Inc Negativstrenget RNA-virusvektor med autonom replikeringsaktivitet
DK0863202T3 (da) 1995-11-01 2010-09-27 Dnavec Research Inc Rekombinant Sendai-virus
US6130313A (en) 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
ATE399206T1 (de) 1999-05-18 2008-07-15 Dnavec Research Inc Ribonukleoproteinkomplex aus paramyxovirus
KR100739938B1 (ko) 1999-05-18 2007-07-16 가부시키가이샤 디나벡크 겐큐쇼 엔벨로프 유전자 결손 파라믹소과 바이러스 벡터
EP1211318B1 (en) 1999-09-06 2007-08-08 DNAVEC Research, Inc. Paramyxoviruses comprising a modified transcription start sequence
JPWO2003025570A1 (ja) 2001-09-18 2004-12-24 株式会社ディナベック研究所 粒子形成能が低下した（−）鎖ｒｎａウィルスベクターの検査方法および製造方法
JP4999330B2 (ja) 2004-01-22 2012-08-15 株式会社ディナベック研究所 サイトメガロウイルスエンハンサーおよびニワトリβ−アクチンプロモーターを含むハイブリッドプロモーターを利用したマイナス鎖ＲＮＡウイルスベクターの製造方法
WO2006137517A1 (ja) 2005-06-24 2006-12-28 Dnavec Corporation 幼少個体への遺伝子導入用ベクター
JP4944781B2 (ja) 2005-09-15 2012-06-06 独立行政法人科学技術振興機構 テトラサイクリン系抗生物質によるタンパク質分解制御法
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
CA2636600A1 (en) 2006-01-17 2007-07-26 Dnavec Corporation Novel protein expression system
KR20090052857A (ko) 2006-07-13 2009-05-26 디나벡크 가부시키가이샤 비복제형 파라믹소바이러스과 바이러스 벡터
US8173792B2 (en) 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
US9127256B2 (en) 2008-07-16 2015-09-08 Dnavec Corporation Method for production of reprogrammed cell using chromosomally unintegrated virus vector
JP5605658B2 (ja) 2009-04-30 2014-10-15 国立大学法人大阪大学 哺乳類細胞におけるタンパク質分解誘導方法
JP5696282B2 (ja) 2009-06-10 2015-04-08 国立大学法人京都大学 人工多能性幹細胞樹立効率改善剤
JP2011004674A (ja) 2009-06-26 2011-01-13 Fujitsu Ltd 誘導多能性幹細胞（ｉＰＳ細胞）の製造方法
JP2014217345A (ja) 2013-05-10 2014-11-20 学校法人 埼玉医科大学 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法
JP2014217344A (ja) 2013-05-10 2014-11-20 学校法人 埼玉医科大学 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法
JP6769880B2 (ja) 2015-02-02 2020-10-14 株式会社Ｉｄファーマ 改良されたマイナス鎖ｒｎａウイルスベクター
WO2019093340A1 (ja) 2017-11-07 2019-05-16 国立大学法人京都大学 ナイーブ型多能性幹細胞からの原始内胚葉誘導方法
JP2020149447A (ja) 2019-03-14 2020-09-17 富士ゼロックス株式会社 表示情報生成装置及びプログラム
US12559730B2 (en) * 2020-01-16 2026-02-24 Riken Mutant KLF protein, and method for producing induced pluripotent stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015519898A (ja) 2012-05-21 2015-07-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 合成自己複製ＲＮＡによるヒトｉＰＳ細胞の作製
WO2017010080A1 (ja) 2015-07-10 2017-01-19 Ｈｅａｒｔｓｅｅｄ株式会社 高品質なｉＰＳ細胞の製造方法
JP2020511529A (ja) 2017-03-03 2020-04-16 オブシディアン セラピューティクス， インコーポレイテッド 免疫療法のためのｃｄ１９組成物及び方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"ｉＰＳ細胞作製キットCytoTuneR-iPS ", ナカライテスク株式会社, [online]，2019年02月，https://www.nacalai.co.jp/products/entry/d012011.html， [2021年11月2日検索］
"ProteoTunerTMシステムを用いた分泌タンパク質レベルの直接調整とノックアウト表現型のレスキューの調節",クロンテック株式会社，［online]，CLONTECHNIQUES 早春号 Technical Note，2009年，30ページ，https://catalog.takara-bio.co.jp/PDFS/200902_30.pdf，[2025年7月9日検索]

Also Published As

Publication number Publication date
JPWO2022050419A1 (https=) 2022-03-10
US20230220025A1 (en) 2023-07-13
EP4209593A4 (en) 2024-10-09
CN116018402A (zh) 2023-04-25
WO2022050419A1 (ja) 2022-03-10
EP4209593A1 (en) 2023-07-12

Similar Documents

Publication Publication Date Title
KR101685209B1 (ko) 유도된 다능성 줄기 세포의 효율적인 확립 방법
EP2526189B1 (en) Method for improving induced pluripotent stem cell generation efficiency
AU2011297075B2 (en) Composition for inducing pluripotent stem cell, and use thereof
US20110003365A1 (en) Method of preparing induced pluripotent stem cells deprived of reprogramming gene
CN105916980B (zh) 用于改进诱导多能干细胞的效率的方法
JP6927578B2 (ja) 高品質なｉＰＳ細胞の製造方法
JP5773393B2 (ja) 効率的な人工多能性幹細胞の樹立方法
JP7808298B2 (ja) ｉＰＳ細胞の品質改善剤、ｉＰＳ細胞の製造方法、ｉＰＳ細胞、及びｉＰＳ細胞製造用組成物
HK40096086A (en) Quality improving agent for ips cells, method of producing ips cells, ips cells, and composition for producing ips cells
JP2014217344A (ja) 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法
WO2011055851A1 (en) Method of efficiently establishing induced pluripotent stem cells
JP2014217345A (ja) 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法
WO2014181682A1 (ja) 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20230303

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240823

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250722

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250922

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20251007

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20251121

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20251209

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20260108

R150 Certificate of patent or registration of utility model

Ref document number: 7808298

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150