JP7472021B2 - ルシフェラーゼ変異体 - Google Patents

ルシフェラーゼ変異体 Download PDF

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Publication number
JP7472021B2
JP7472021B2 JP2020529058A JP2020529058A JP7472021B2 JP 7472021 B2 JP7472021 B2 JP 7472021B2 JP 2020529058 A JP2020529058 A JP 2020529058A JP 2020529058 A JP2020529058 A JP 2020529058A JP 7472021 B2 JP7472021 B2 JP 7472021B2
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Prior art keywords
amino acid
luciferase
seq
mutant
acid sequence
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Japanese (ja)
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JPWO2020009215A1 (ja
JPWO2020009215A5 (https=
Inventor
華奈子 林
敦 一柳
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Kikkoman Corp
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Kikkoman Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2020529058A 2018-07-06 2019-07-05 ルシフェラーゼ変異体 Active JP7472021B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018129384 2018-07-06
JP2018129384 2018-07-06
PCT/JP2019/026784 WO2020009215A1 (ja) 2018-07-06 2019-07-05 ルシフェラーゼ変異体

Publications (3)

Publication Number Publication Date
JPWO2020009215A1 JPWO2020009215A1 (ja) 2021-07-15
JPWO2020009215A5 JPWO2020009215A5 (https=) 2022-07-08
JP7472021B2 true JP7472021B2 (ja) 2024-04-22

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ID=69060439

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020529058A Active JP7472021B2 (ja) 2018-07-06 2019-07-05 ルシフェラーゼ変異体

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US (1) US20220064606A9 (https=)
JP (1) JP7472021B2 (https=)
WO (1) WO2020009215A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7385134B2 (ja) * 2021-03-12 2023-11-22 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法
CN120457210A (zh) 2023-01-27 2025-08-08 龟甲万株式会社 萤光素酶突变体
CN119776293B (zh) * 2024-12-06 2025-11-04 上海交通大学 一种ArsL突变体及其在合成Arsinothricin中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516585A (ja) 1997-09-19 2001-10-02 プロメガ・コーポレイシヨン 熱安定ルシフェラーゼと生産方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5229285A (en) * 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
EP2221386B1 (en) * 2007-11-20 2012-06-20 Kikkoman Corporation Composition for analysis of nucleic acid
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ
JP2011120559A (ja) * 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516585A (ja) 1997-09-19 2001-10-02 プロメガ・コーポレイシヨン 熱安定ルシフェラーゼと生産方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DEMENTIEVA, EI. et al.，Physicochemical Properties of Recombinant Luciola mingrelica Luciferase and Its Mutant Forms，Biochemistry(Moscow)，1996年，Vol.61, No.1，p.115-119，Abstract, TABLE1, Fig.1, p.118 left column line 21 - right column line 16
HATTORI N. et al.，Mutant Luciferase Enzymes from Fireflies with Increased Resistance to Benzalkonium Chloride，Bioscience, Biotechnology, and Biochemistry，2002年，Vol.66, No.12，p.2587-2593，Abstract, Materials and Methods, Fig.1-3, Table 1-2

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Publication number Publication date
US20220064606A9 (en) 2022-03-03
WO2020009215A1 (ja) 2020-01-09
JPWO2020009215A1 (ja) 2021-07-15
US20210171919A1 (en) 2021-06-10

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